• International Journal of Stem Cells
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• v.17 no.3
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• pp.330-336
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• 2024

Mesenchymal stem cells in the dental tissue indicate a disposition for differentiation into diverse dental lineages and contain enormous potential as the important means for regenerative medicine in dentistry. Among various dental tissues, the dental pulp contains stem cells, progenitor cells and odontoblasts for maintaining dentin homeostasis. The conventional culture of stem cells holds a limit as the living tissue constitutes the three-dimensional (3D) structure. Recent development in the organoid cultures have successfully recapitulated 3D structure and advanced to the assembling of different types. In the current study, the protocol for 3D explant culture of the human dental pulp tissue has been established by adopting the organoid culture. After isolating dental pulp from human tooth, the intact tissue was placed between two layers for Matrigel with addition of the culture medium. The reticular outgrowth of pre-odontoblast layer continued for a month and the random accumulation of dentin was observed near the end. Electron microscopy showed the cellular organization and in situ development of dentin, and immunohistochemistry exhibited the expression of odontoblast and stem cell markers in the outgrowth area. Three-dimensional explant culture of human dental pulp will provide a novel platform for understanding stem cell biology inside the tooth and developing the regenerative medicine.

• The Research Journal of the Costume Culture
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• v.24 no.6
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• pp.725-742
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• 2016

In the Korean fashion industry, 3D printing systems are considered as new technology and a new opportunity. With 3D printers, consumers can be manufacturers and individuals can develop businesses with little upfront capital. In this study, a dress form for the typical Korean women's body shape was developed using 3D technology (3D scanning, 3D modeling, and 3D printing). Ten women with apparel sizes 85-91-160 were selected from 3D body-scan data collected by SizeKorea of 201 women aged 25 to 34 (2010). First, 15 horizontal cross-sections were collected from the 3D scan data of the 10 subjects. Then, inside lines of those cross-sections were drawn at 15-degree intervals, and the lengths were measured. The average of the inside lines was connected to the internal spline curve, and the curves were used as the average cross-sections. The average torso body and the dress form of Korean women were developed into a 3D solid model using a 3D CAD program (Solidworks 2012). An output mockup was printed by the FDM type's 3D printing system (Bonbot 1200, Bonbot 3-H4) using PLA material. The dimensions comparing the 3D solid modeling to the 3D printed mockup of the dress form were measured, and minor differences were between 0.00cm and 0.40cm. In the future, 3D printing systems are expected to be in use for various personalized dress forms.

• Archives of Pharmacal Research
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• v.27 no.9
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• pp.912-914
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• 2004

Seven compounds were isolated from the stem of Acanthopanax senticosus by repeated col-umn chromatography. Their structures were elucidated as isovanillin (1), (-)-sesamin (2), iso-fraxidin (3), (＋)-syringaresinol (4), 5-hydroxymethylfurfural (5), eleutheroside B (6), and eleuth-eroside E (7) by spectral analysis. Among them, isovanillin (1) was isolated for the first time from the family Araliaceae.

• International Journal of Advanced Culture Technology
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• v.1 no.1
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• pp.19-22
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• 2013

The basic principle of the product manufacturing technology using the 3D printing technique materializes the material including the high molecular substance or plastic and metallic dust, and etc. the product into the laminate additive manufacturing according to the design diagram gradually. It is applied to the various industrial field including the field of food division, field of home appliances, field of medicine, field of mechanical department and construction, etc.. The global development case of 3D printing technique is the next. This study described global technology and market trends. Afterward, 3D printing technique manages the important role when it exceeds the product manufacturing view just and is grafted with the various technology including the biotechnology, nanotechnology, and etc. and it improves the quality of the human life.

• Proceedings of the Costume Culture Conference
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• 2000.09a
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• pp.69-69
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• 2000

• Proceedings of the Costume Culture Conference
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• 2001.04a
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• pp.23-24
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• 2001

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.6
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• pp.349-353
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• 2003

The physiological characteristics of cultures of very high cell mass (e.g. 10g cell mass/L), termed“ultrahigh cell density cultures”is reviewed. A close relationship was found between the length of the optical path (OP) in flat-plate reactors and the optimal cell density of the culture as well as its areal (g m$\^$-2/ day$\^$-1/) productivity. Cell-growth inhibition (GI) unfolds as culture density surpasses a certain threshold. If it is constantly relieved, a 1.0cm OP reactor could produce ca. 50% more than reactors with longer OP, e.g. 5 or 10cm. This unique effect, discovered by Hu et al. [3], is explained in terms of the relationships between the frequency of the light-dark cycle (L-D cycle), cells undergo in their travel between the light and dark volumes in the reactor, and the turnover time of the photosynthetic center (PC). In long OP reactors (5cm and above) the L-D cycle time may be orders of magnitude longer than the PC turnover time, resulting in a light regime in which the cells are exposed along the L-D cycle, to long, wasteful dark periods. In contrast, in reactors with an OP of ca. 1.0 cm, the L-D cycle frequency approaches the PC turnover time resulting in a significant reduction of the wasteful dark exposure time, thereby inducing a surge in photosynthetic efficiency. Presently, the major difficulty in mass cultivation of ultrahigh-density culture (UHDC) concerns cell growth inhibition in the culture, the exact nature of which is awaiting detailed investigation.

• Proceedings of the Korean Society of Computer Information Conference
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• 2019.07a
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• pp.367-370
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• 2019

본 논문에서는 사료 검증을 바탕으로 반차도의 문화원형(文和元型, Culture Archetype) 가상복원을 통한 3D 증강현실 콘텐츠를 개발하였다. 반차도란 의궤에 담긴, 조선 시대 왕실의 행차를 그린 그림을 의미한다. 조선의 왕실문화를 활용한 궁궐콘텐츠는 한국 고유의 것이며, 스토리텔링이 풍부한 반차도를 활용한다면 국민의 전통문화 향유를 증대시킬 수 있다. 현대에서는 재현행사를 통해 왕실의 행차를 재현하고 있지만, 실내에서는 재현하기 어려워 왕실 행차에 대한 문화유산 접근성이 낮다는 한계가 있다. 또한, 문화콘텐츠닷컴과 국립중앙박물관에서 제공하는 반차도 관련 멀티미디어 콘텐츠는 반차도 구성을 설명하는 것이 아니므로 심도 있는 반차도 학습에 어려움이 따른다. 이를 극복하고자 본 논문에서는 사료 고증 메타데이터에 기반을 두어 문화원형 가상복원 3D 증강현실 콘텐츠를 제안하고자 한다. 이를 토대로 문화원형 가상복원을 위한 3D 증강현실 콘텐츠의 활용 가능 모습을 살펴보고, 기록유산 반차도의 문화원형 가상복원기반 증강현실 3D 콘텐츠를 제안하다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.10
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• pp.551-558
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• 2017

To study and validate tissue-specific promoters and vectors, it is important to develop cell culture systems that retain the tissue and species specificity. Such systems are attractive alternatives to transgenic animal models. This study established a line of porcine mammary gland epithelial cells (PMECs) from a primary culture based on the cellular morphology and mRNA levels of porcine beta-casein (CSN2). The selected PMECs were stained with the cytokeratin antibody, and were shown to express milk protein genes (CSN2, lactoferrin, and whey acidic protein). In addition, to confirm the acini structure of PMEC932-7 in 3D culture, live cells were stained with SYTO-13 dye, which binds to nucleic acid. The acini of these PMECs on matrigel were formed by the aggregation of peripheral cells and featured a hollow lumens. The system was demonstrated by testing the effects of the culture conditions to cell culture including cell density and matrigel methods of the PMECs. These results suggest that PMECs possess the genetic and structural features of mammary epithelial cells.

• The Research Journal of the Costume Culture
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• v.15 no.3 s.68
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• pp.446-460
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• 2007

This study shows that digital technology is adapted practical method in fashion design process and virtual simulation and cyber fashion gallery based on virtual reality are researched. This study is proposed the 3D fashion design simulation in the virtual space used on 3D studio max, poser, photoshop program according to fashion design process. The main design concept is "temporary bridge" from rainbow. This study is supposed six fashion design in accordance with three sub-theme under main concept by changing color and texture used on 3D simulation. The results of this study are as follows: 1. This study produced Cyber Fashion Gallery in virtual space to the form of CD Rom title and web title by Macromedia Director 8.5, Macromedia Flash, Sound Forge. And it is enlarged the field of expression in aspect of Fashion Exhibition, beyond restriction of time and space. 2. Clothes modelling tools is able to easily adapt to various textiles and patterns in 3D dynamic virtual mannequin before making clothes. Digital technology is able to express image changed color and texture, especially new material, multi-finishing material and brilliant material and so on. So this study is able to develop tools for study of fashion coordination. 3. Cyber Fashion Gallery consists of gallery, story, painting, symbolism, example, image, quit. This study is enlarged the range of clothing expression by digital technology and open to possibility customized-manufacture.