• Title/Summary/Keyword: 3-D Mechanism

Search Result 1,731, Processing Time 0.029 seconds

Quercetin-3-O-β-D-Glucuronide Suppresses Lipopolysaccharide-Induced JNK and ERK Phosphorylation in LPS-Challenged RAW264.7 Cells

  • Park, Jin-Young;Lim, Man-Sup;Kim, Song-In;Lee, Hee Jae;Kim, Sung-Soo;Kwon, Yong-Soo;Chun, Wanjoo
    • Biomolecules & Therapeutics
    • /
    • v.24 no.6
    • /
    • pp.610-615
    • /
    • 2016
  • Quercetin, a flavonol, has been reported to exhibit a wide range of biological properties including anti-oxidant and anti-inflammatory activities. However, pharmacological properties of quercetin-3-O-${\beta}$-D-glucuronide (QG), a glycoside derivative of quercetin, have not been extensively examined. The objective of this study is to elucidate the anti-inflammatory property and underlying mechanism of QG in lipopolysaccharide (LPS)-challenged RAW264.7 macrophage cells in comparison with quercetin. QG significantly suppressed LPS-induced extracellular secretion of pro-inflammatory mediators such as nitric oxide (NO) and $PGE_2$, and pro-inflammatory protein expressions of iNOS and COX-2. To elucidate the underlying mechanism of the anti-inflammatory property of QG, involvement of MAPK signaling pathways was examined. QG significantly attenuated LPS-induced activation of JNK and ERK in concentration-dependent manners with a negligible effect on p38. In conclusion, the present study demonstrates QG exerts anti-inflammatory activity through the suppression of JNK and ERK signaling pathways in LPS-challenged RAW264.7 macrophage cells.

Inactivation Mechanism of Bacillus subtilis Spores by Ethanol Extract of Torilis japonica Fruit

  • Cho, Won-Il;Cheigh, Chan-Ick;Choi, You-Jung;Jeong, Jeong-Yoon;Choi, Jun-Bong;Lee, Kang-Pyo;Cho, Seok-Cheol;Pyun, Yu-Ryang;Chung, Myong-Soo
    • Food Science and Biotechnology
    • /
    • v.18 no.2
    • /
    • pp.336-342
    • /
    • 2009
  • To confirm the antimicobial mechanism of Torilis japonica, antimicrobial profile was observed on various spore conditions by combining 0.1% (3 mM) torilin with antimicrobial activity and 0.27% water fraction with germinants. A 75% ethanol extract of T. japonica fruit reduced Bacillus subtilis ATCC 6633 spore counts by 3 log cycles and reduced the vegetative cells to undetectable level (by about 6 log cycles) (both in terms of CFU/mL). Further fractionating the ethanol extract into n-hexane and water fractions revealed that the former reduced the spore count by 1 log cycle whereas the latter had no effect. The antimicrobial active compound was isolated and purified from the hexane layer, and identified as torilin ($C_{22}H_{32}O_5$). The water fraction of the ethanol layer did not show antimicrobial activity, whereas the antimicrobial effect of 0.1% (3 mM) torilin was significantly enhanced in the presence of the water fraction (0.27%). This result can be explained by synergistic effects of the water fraction containing considerable amounts of germinants such as L-alanine and K+ ions that triggered germination.

Simulation of fracture mechanism of pre-holed concrete model under Brazilian test using PFC3D

  • Sarfarazi, Vahab;Haeri, Hadi;Shemirani, Alireza Bagher
    • Smart Structures and Systems
    • /
    • v.22 no.6
    • /
    • pp.675-687
    • /
    • 2018
  • In the previous studies on the porous rock strength the effect of pore number and its diameter is not explicitly defined. In this paper crack initiation, propagation and coalescence in Brazilian model disc containing a single cylindrical hole and or multiple holes have been studied numerically using PFC3D. In model with internal hole, the ratio of hole diameter to model diameter was varied between 0.03, 0.17, 0.25, 0.33, and 0.42. In model with multiple hole number of holes was different in various model, i.e., one hole, two holes, three holes, four holes, five holes, six holes, seven holes, eight holes and nine holes. Diameter of these holes was 5 mm, 10 mm and 12 mm. The pre-holed Brazilian discs are numerically tested under Brazilian test. The breakage load in the ring type disc specimens containing an internal hole with varying diameters is measured. The mechanism of cracks propagation in the wall of the ring type specimens is also studied. In the case of multi-hole Brazilian disc, the cracks propagation and b cracks coalescence are also investigated. The results shows that breaking of the pre-holed disc specimens is due to the propagation of radially induced tensile cracks initiated from the surface of the central hole and propagating toward the direction of diametrical loading. In the case of disc specimens with multiple holes, the cracks propagation and cracks coalescence may occur simultaneously in the breaking process of model under diametrical compressive loading. Finally the results shows that the failure stress and crack initiation stress decreases by increasing the hole diameter. Also, the failure stress decreases by increasing the number of hole which mobilized in failure. The results of these simulations were comprised with other experimental and numerical test results. It has been shown that the numerical and experimental results are in good agreement with each other.

Fuzzy Control and Implementation of a 3-Dimensional Inverted Pendulum System (3차원 도립진자 시스템의 구현 및 퍼지 제어)

  • Shin, Ho-Sun;Chu, Jun-Uk;Lee, Seung-Ha;Lee, Yun-Jung
    • Journal of the Korean Institute of Intelligent Systems
    • /
    • v.13 no.2
    • /
    • pp.137-147
    • /
    • 2003
  • The fuzzy control and implementation of a new three-dimensional(3-D) inverted pendulum system are addressed. In comparison with conventional 1-D and 2-D systems, the 3-D inverted pendulum system is a proper benchmark system to simulate human's control action which includes the up and down motion to stabilize an inverted pendulum. To investigate the characteristics of the 3-D inverted pendulum system and to design of a fuzzy controller, we derive dynamic equations of the mechanism including a 3-axis cartesian robot and an inverted pendulum. We propose a design method of a fuzzy controller of the yaw and pitch angles of an inverted pendulum. In the design, the redundant degree-of-freedom(DOF) of the robot and the constrained workspace are taken into account. The performance of the proposed system is proved by experimental results using a developed PC-based Multi-Motion Control(MMC) board.

A Study on the Electrical Conduction and D.C. Breakdown Properties of $(Sr{\cdot}Pb)TiO_3$ Series Ceramic ($(Sr{\cdot}Pb)TiO_3$계 세라믹의 전기전도 및 절연파괴 특성에 관한 연구)

  • Jung, I.H.;Choi, W.S.;Kim, C.H.;Lee, J.U.
    • Proceedings of the KIEE Conference
    • /
    • 1991.11a
    • /
    • pp.321-324
    • /
    • 1991
  • In this study, $(Sr{\cdot}Pb)TiO_3$ series ceramics which is used in high voltage were fabricated by the mixed oxide method, and the electrical conduction mechanism and D.C. breakdown voltage characteristics of the specimens in accordance with the contents of $Bi_2O_3{\cdot}3TiO_2$ were investigated. As a result, the leakage current was increased with the contents of $Bi_2O_3{\cdot}3TiO_2$ and the measuring temperature. At room temperature, the leakeage current was showed a tendency of saturating when D.C. electrical field of $l5{\sim}30$[kV/cm] was applied to the specimen. As a result of breakdown voltage characteristics. breakdown strength was decreased when the contents of $Bi_2O_3{\cdot}3TiO_2$ were increased. On the other hand, in the temperature region below $60[^{\circ}C]$, the electronic breakdown was occured, and in the temperature region from 60 to $200[^{\circ}C]$, the thermal breakdown was occured by the Joule heat and the dissipation factor.

  • PDF

Nucleophilic Substitutions at a Carbonyl Carbon Atom (ⅩⅡ). Solvolysis of Methylchloroformate and Its Thioanalogues in $CH_3CN-H_2O$ and $CH_3COCH_3-H_2$ Mixtures (카르보닐탄소원자의 친핵성 치환반응 (제 12 보).아세토니트릴-물 및 아세톤-물 혼합용 매속에서 메틸클로로훠메이트와 그 티오유도체들의 가용매분해반응에 관한 연구)

  • Sangmoo La;Kyeong Shin Koh;Ikchoon Lee
    • Journal of the Korean Chemical Society
    • /
    • v.24 no.1
    • /
    • pp.8-14
    • /
    • 1980
  • Solvolysis rate constants for methylchloroformate, $CH_3O$(CO)Cl, methylthiono-chloroformate, $CH_3O$(CS)Cl, and methylthiolchloroformate, $CH_3S$(CO)Cl, have been determined conductometrically in acetone-water and acetonitrile-water mixtures, and activation parameters, ${\Delta}H^{\neq}$ and ${\Delta}S^{\neq}$, have been derived. Results show that in water-rich regions the order of rate increases as $$CH_3O(CO)Cl while in dipolar aprotic solvent-rich region this order reverses. The plots of log k vs. solvent parameters, Y, $\frac{D-1}{2D+1}$ and log($H_2$) show that the order of rate increase in water-rich region is the results of increase in $S_N1$ character. It is concluded that $CH_3S$(CO)Cl solvolyzes via $S_N1$ mechanism whereas $CH_3O$(CO)Cl reacts via $S_N2$ and $CH_3O$(CS)Cl via intermediate mechanism in water-rich region.

  • PDF

Serum 25-hydroxyvitamin D3 is associated with homocysteine more than with apolipoprotein B

  • Nam-Kyu, Kim;Min-Ah, Jung;Beom-hee, Choi;Nam-Seok, Joo
    • Nutrition Research and Practice
    • /
    • v.16 no.6
    • /
    • pp.745-754
    • /
    • 2022
  • BACKGROUND/OBJECTIVES: The incidence of cardiovascular diseases (CVDs) has increased worldwide. Although a low serum vitamin D level is known to be associated with the risk of CVD, the mechanism is not well understood yet. The aim of this study was to determine the relationship of serum 25-hydroxyvitamin D3 (25[OH]D) with homocysteine and apolipoprotein B (ApoB). SUBJECTS/METHODS: Of 777 subjects recruited from one health promotion center for routine heath exam from January 2010 to December 2016, 518 subjects were included in this study. Serum 25(OH)D, serum homocysteine, and other metabolic parameters including ApoB were analyzed. Simple and partial correlations were carried out after adjustments. Simple linear regression analysis was used for precise correlation of parameters. Multivariate regression analysis was done to know which factor (serum homocysteine or ApoB) was more related to serum 25(OH)D after adjustments. Finally, logarithms of homocysteine concentrations according to tertiles of serum 25(OH)D were compared. RESULTS: After sex and age adjustments, serum 25(OH)D showed negative correlations with serum homocysteine (r' = -0.114) and ApoB (r' = -0.098). In simple linear regression analysis, serum 25(OH)D showed a significant negative correlation with ApoB (P = 0.035). However, in multivariate regression analysis, serum 25(OH)D was significantly associated with serum homocysteine after adjustments (P = 0.022). In addition, serum homocysteine concentration was significantly high in the lowest 25(OH)D group (P = 0.046). CONCLUSION: Serum 25(OH)D concentration showed a stronger negative association with serum homocysteine than with ApoB.

Kinetics and Mechanism of the Anilinolysis of (2R,4R,5S)-(+)-2-Chloro-3,4-dimethyl -5-phenyl-1,3,2-oxazaphospholidine 2-Sulfide in Acetonitrile

  • Barai, Hasi Rani;Lee, Hai-Whang
    • Bulletin of the Korean Chemical Society
    • /
    • v.33 no.3
    • /
    • pp.1037-1041
    • /
    • 2012
  • The nucleophilic substitution reactions of (2R,4R,5S)-(+)-2-chloro-3,4-dimethyl-5-phenyl-1,3,2-oxazaphospholidine 2-sulfide (3) with substituted anilines ($XC_6H_4NH_2$) and deuterated anilines ($XC_6H_4ND_2$) are investigated kinetically in acetonitrile at $5.0^{\circ}C$. The anilinolysis rate of 3 involving a cyclic five-membered ring is considerably fast because of small negative value of the entropy of activation (${\Delta}S^\neq=-2cal\;mol^{-1}\;K^{-1}$) over considerably unfavorable enthalpy of activation (${\Delta}H^\neq=18.0\;kcal\;mol^{-1}$). Great enthalpy and small negative entropy of activation are ascribed to sterically congested transition state (TS) and bulk solvent structure breaking in the TS. A concerted $S_N2$ mechanism with a backside nucleophilic attack is proposed on the basis of the secondary inverse deuterium kinetic isotope effects, $k_H/k_D$ < 1.

A Simplified 3D-Location Scheme for Wireless Sensor Networks (무선 센서 네트워크를 위한 저복잡도 3차원 무선측위 기법)

  • Hoang, Quan T.;Kim, Kwang-Yul;Shin, Yo-An
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.36 no.9C
    • /
    • pp.573-580
    • /
    • 2011
  • WSNs (Wireless Sensor Networks) are becoming more widely used in various fields, and improving localization performance is a crucial and essential issue for sensor network applications. In this paper, we propose a low-complexity localization mechanism for WSNs that operates in 3D (Three-Dimensional) space. The basic idea is to use aerial vehicles or flying objects that are deliberately equipped with the anchor nodes. These anchor nodes periodically broadcast beacon signals containing their current locations, and the unknown nodes receive these signals as soon as they enter the communication range of the anchors. We estimate the locations of the unknown nodes based on the proposed scheme that transforms the 3D problem into 2D computations to reduce the complexity of 3D localization. Simulated results show that our approach is an effective scheme for 3D self-positioning in WSNs.

Inhibitory mechanism of ginsenoside Rh3 on granulocyte-macrophage colony-stimulating factor expression in UV-B-irradiated murine SP-1 keratinocytes

  • Park, Young Sun;Lee, Ji Eun;Park, Jong Il;Myung, Cheol hwan;Lim, Young-Ho;Park, Chae Kyu;Hwang, Jae Sung
    • Journal of Ginseng Research
    • /
    • v.44 no.2
    • /
    • pp.274-281
    • /
    • 2020
  • Background: Ultraviolet (UV) goes through the epidermis and promotes release of inflammatory cytokines in keratinocytes. Granulocyte-macrophage colony-stimulating factor (GM-CSF), one of the keratinocyte-derived cytokines, regulates proliferation and differentiation of melanocytes. Extracellular signal-regulated kinase (ERK1/2) and protein kinase C (PKC) signaling pathways regulate expression of GM-CSF. Based on these results, we found that ginsenoside Rh3 prevented GM-CSF production and release in UV-B-exposed SP-1 keratinocytes and that this inhibitory effect resulted from the reduction of PKCδ and ERK phosphorylation. Methods: We investigated the mechanism by which ginsenoside Rh3 from Panax ginseng inhibited GM-CSF release from UV-B-irradiated keratinocytes. Results: Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) or UV-B induced release of GM-CSF in the SP-1 keratinocytes. To elucidate whether the change in GM-CSF expression could be related to PKC signaling, the cells were pretreated with H7, an inhibitor of PKC, and irradiated with UV-B. GM-CSF was decreased by H7 in a dose-dependent manner. When we analyzed which ginsenosides repressed GM-CSF expression among 15 ginsenosides, ginsenoside Rh3 showed the largest decline to 40% of GM-CSF expression in enzyme-linked immunosorbent assay. Western blot analysis showed that TPA enhanced the phosphorylation of PKCδ and ERK in the keratinocytes. When we examined the effect of ginsenoside Rh3, we identified that ginsenoside Rh3 inhibited the TPA-induced phosphorylation levels of PKCδ and ERK. Conclusion: In summary, we found that ginsenoside Rh3 impeded UV-B-induced GM-CSF production through repression of PKCδ and ERK phosphorylation in SP-1 keratinocytes.