• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4337-4340
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• 2011

The vitamin D receptor binding peptide, VDRBP, was overexpressed as a fused form with the ubiquitin molecule in Rosetta(DE3)pLysS, a protein production strain of Escherichia coli harboring an induction controller plasmid. The fusion protein was bound to the immobilized metal ions, and the denaturation and renaturation of the fusion protein were performed as a part of the purification procedure. After the elution of the fusion protein, the peptide hormone was released from its fusion partner by using yeast ubiquitin hydrolase (YUH), and subsequently purified by reverse phase chromatography. The purity of the resulting peptide fragment was checked by MALDI-TOF mass and NMR spectroscopy. The final yields of the target peptide were around 5 and 2 mg per liter of LB and minimal media, respectively. The recombinant expression and purification of this peptide will enable structural and functional studies using multidimensional NMR spectroscopy and X-ray crystallography.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 1999년도 추계학술대회
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• pp.565-574
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• 1999

In reverse engineering area, it is rapidly developing reconstruction of surfaces from scanning or digitizing data, but geometric models of existing objects unavailable many industries. This paper describes new methodology of reverse engineering area, good strategies and important algorithms in reverse engineering area. Furthermore, proposing reconstruction of surface technique is presented. A method find base geometry and blending surface between them. Each based geometry is divided by triangular patch which are compared their normal vector for face grouping. Each group is categorized analytical surface such as a part of the cylinder, the sphere, the cone, and the plane that mean each based geometry surface. And then, each based geometry surface is implemented infinitive surface. Infinitive average surface's intersections are trimmed boundary representation model reconstruction. This method has several benefits such as the time efficiency and automatic functional modeling system in reverse engineering. Especially, it can be applied 3D scanner and 3D copier.

• Journal of Mechanical Science and Technology
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• 제20권10호
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• pp.1680-1690
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• 2006

As a promising and novel manufacturing technology, laser aided direct metal deposition (DMD) process produces near-net-shape functional metal parts directly from 3-D CAD models by repeating laser cladding layer by layer. The key of the build-up mechanism is the effective control of powder delivery and laser power to be irradiated into the melt-pool. A feedback control system using two sets of optical height sensors is designed for monitoring the melt-pool and real-time control of deposition dimension. With the feedback height control system, the dimensions of part can be controlled within designed tolerance maintaining real time control of each layer thickness. Clad nugget shapes reveal that the feedback control can affect the nugget size and morphology of microstructure. The pore/void level can be controlled by utilizing pulsed-mode laser and proper design of deposition tool-path. With the present configuration of the control system, it is believed that more innovation of the DMD process is possible to the deposition of layers in 3-D slice.

• 산업공학
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• 제12권2호
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• pp.205-209
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• 1999

In reverse engineering area, the reconstruction of surfaces from scanned or digitized data is being developed, but geometric model of existing objects is not available in industries. This paper presents the new approach to the reconstruction of surface technique. A proposed methodology finds base geometry and blends surface between them. Each based geometry is divided by tri-angular patches which are compared with their normal vector for face grouping. Each group is categorized analytical surface such as a part of cylinder, sphere and cone, and plane shapes to represent the based geometry surface. And then, each based geometry surface is implemented to the infinitive surface. Infinitive surface's intersections are trimmed by boundary representation model reconstruction. This method has several benefits such as time efficiency and automatic functional modeling system in reverse engineering. Especially, it can be directly applied 3D fax and 3D copier.

• Coupled systems mechanics
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• 제10권6호
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• pp.521-544
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• 2021

In this work, we present the development of a 3D lattice-type model at microscale based upon the Voronoi-cell representation of material microstructure. This model can capture the coupling between mechanic and electric fields with non-linear constitutive behavior for both. More precisely, for electric part we consider the ferroelectric constitutive behavior with the possibility of domain switching polarization, which can be handled in the same fashion as deformation theory of plasticity. For mechanics part, we introduce the constitutive model of plasticity with the Armstrong-Frederick kinematic hardening. This model is used to simulate a complete coupling of the chosen electric and mechanics behavior with a multiscale approach implemented within the same computational architecture.

• 정보처리학회논문지D
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• 제16D권3호
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• pp.327-338
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• 2009

마이크로어레이 데이터는 유전자의 집합이 어떠한 조건 혹은 샘플의 집합 하에서 얼마나 발현되는지를 수치화한 2차원 행렬 데이터이다. 바이클러스터는 마이크로어레이의 샘플의 부분 집합과 이 샘플 부분 집합 하에서 일정한 증감 패턴을 보이는 유전자의 부분 집합을 말한다. 이렇게 같은 패턴을 보이는 유전자의 부분 집합은 일정한 정도의 유의 수준으로 비슷한 기능을 한다고 말할 수 있다. 따라서 바이클러스터링 알고리즘은 같은 기능에 연관된 유전자의 집합과, 이 기능이 발현되고 있는 조건의 집합을 밝혀내는데 있어서 매우 유용하다. 본 논문에서는 다항식 시간 복잡도를 유지하면서, 높은 기능적 상관관계를 가지는 바이클러스터를 밝혀 낼 수 있는 알고리즘을 제안한다. 이 알고리즘은 1) 마이크로어레이 데이터에 심한 노이즈가 있을 경우 패턴으로 인식하지 못하는 기존 알고리즘과 달리, 노이즈 레벨이 심하더라도 거시적으로 비슷한 모양을 보이는 패턴을 찾아내는 방식을 이용하여 숨어있는 패턴들을 찾아낼 수 있고, 2) 바이클러스터 상호간에 오버랩을 허용하며, 또한 다양성이 보장되는 복수의 바이클러스터를 찾아내며, 3) 찾아진 유전자 부분 집합의 기능적 상관관계가 매우 높은 특성을 지니고, 4) 유전자 및 샘플의 순서와 상관없이 결정적인(deterministic) 결과를 도출한다. 또한 본 논문에서는 알고리즘이 찾아낸 바이클러스터의 기능적 상관관계의 정도와, 비교 알고리즘이 찾아낸 바이클러스터의 기능적 상관관계의 정도를 유전자 온톨로지(Gene Ontology)를 통해서 측정함으로써 비교하고 있다.

• Macromolecular Research
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• 제15권3호
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• pp.234-237
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• 2007

3,4-Bis-(3,4-dicarboxyphenylcarboxyethoxy)-4'-nitrostilbene dianhydride was prepared and reacted with 4,4'-(hexafluoroisopropylidene)dianiline to yield a novel Y-type polyimide containing the 3,4-dioxynitrostilbenyl group as an NLO-chromophore, which constituted part of the polymer backbone. The resulting polyimide was soluble in polar solvents such as acetone and N,N-dimethylformamide. The polymer exhibited good thermal stability up to $370^{\circ}C$ in the thermogravimetric analysis. The glass-transition temperature ($T_g$) obtained from the differential scanning calorimetry thermogram was near to $153^{\circ}C$. The second harmonic generation (SHG) coefficient ($d_{33}$) of the poled polymer film at the fundamental wavelength of $1064\;cm^{-1}$ was around $2.15\;{\times}\;10^{-8}\;esu$ (9.01 pm/V). The dipole alignment exhibited exceptionally high thermal stability even at a temperature $30^{\circ}C$ above the $T_g$, and there was no SHG decay below $180^{\circ}C$ because of the partial main chain character of the polymer structure.

• 한국기계가공학회지
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• 제19권5호
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• pp.45-52
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• 2020

Recently, design for additive manufacturing (DfAM) technology has become a prominent design methodology for exploiting 3D printing, which leads the Fourth Industrial Revolution. When manufactured by the 3D printing method, it is possible to produce several shapes compared to the conventional casting or cutting process. DfAM-as a newly-proposed design methodology-can be used to specially design products with various shapes to apply functional requirements. Topology optimization verifies load paths to determine the draft design, and a shape-optimized design with objective functions for weight reduction enables efficient lightweight product design. In this study, by using these two DfAM technologies, a lightweight and optimal design is constructed for a node part of a vehicle body with a space frame designed for a lightweight vehicle. DfAM methodologies for concept design and detailed design, and the associated results, are presented. Finally, the product was additively manufactured, a fatigue performance test was performed, and the design reliability was verified.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2011년도 추계학술대회 논문집
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• pp.463-468
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• 2011

According to a quantum leap of the performances of automobile, environmental factors are important as functional factors, especially noise. BLDC motor, one of the part of automobile, is also no exception. In this paper, investigation of the sound sources of outer rotor type BLDC motor is performed. In order to reduce noise, it must be necessary knowing sound source. To this end, this paper is analyzed two viewpoints, structural and electromagnetic causes. For structural analysis, modal experiment and 3D mode analysis are performed. On behalf of electromagnetic analysis, 2D finite element method is carried out. Finally, coupling analysis is performed in order to know about influence between two factors.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 하계학술대회 논문집 B
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• pp.1331-1333
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• 2004

There are many types of BOM, such as EBOM, MBOM, As-Built BOM, As-Designed BOM, Generic BOM, Customized BOM, etc. We should define an adequate BOM to achieve some purposes. In this paper, we proposed BOM structure for EMU Maintenance Information System. First of all, we define Master BOM including all part's information, such as 2D/3D drawings, images, specification, etc. And then we define Object BOM that is functional structure for someone to achieve maintenance works. Object BOM will be applied to configure sales orders, explode aggregate forecasts, and calculate standard cost.