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Analysis of Quantitative Trait Loci (QTLs) for Seed Size and Fatty Acid Composition Using Recombinant Inbred Lines in Soybean (콩 재조합자식계통을 이용한 콩 종자의 크기와 지방산 조성의 양적 형질 유전자좌 분석)

  • Kim, Hyeun-Kyeung;Kim, Yong-Chul;Kim, Sun-Tae;Son, Beung-Gu;Choi, Yong-Whan;Kang, Jum-Soon;Park, Young-Hoon;Cho, Young-Son;Choi, In-Soo
    • Journal of Life Science
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    • v.20 no.8
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    • pp.1186-1192
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    • 2010
  • Soybean [Glycine max(L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvements in economic traits, such as quality and oil constituents, arethe most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control seed size and fatty acid contents in soybean. 115 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Iksan10' were used. Narrow-sense heritability estimates based on a plot mean on 100 seed weight, saturated fatty acid (palmitic acid + stearic acid), and oleic, linoleic, and linolenic acid content were 0.72, 0.60, 0.83, 0.77 and 0.81, respectively. The 100 seeds weight was related to seven QTLs located on chromosomes 1, 3, 8, 9, 16 and 17. Two independent QTLs for saturated fatty acid content were identified on chromosomes 17 and 19. Five independent QTLs for oleic acid content wereidentified on chromosomes7, 11, 14, 16 and 19. Five QTLs for linoleic acid content were located on chromosomes 2, 11, 14, 16 and 19. Three QTLs for linolenic acid content were located on chromosomes 8, 10 and 19. Oleic, linoleic, and linolenic acid had one major common QTL on chromosome 19. Thus, linoleic and linolenic acid content were identified as common QTLs.

Life Table Analysis of the Cabbage Aphide, Brevicoryne brassicae (Linnaeus) (Homoptera: Aphididae), on Tah Tsai Chinese Cabbages (다채를 기주로 양배추가루진딧물[Brevicoryne brassicae (Linnaeus)]의 생명표 분석)

  • Kim, So Hyung;Kim, Kwang-Ho;Hwang, Chang-Yeon;Lim, Ju-Rak;Kim, Kang-Hyeok;Jeon, Sung-Wook
    • Korean journal of applied entomology
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    • v.53 no.4
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    • pp.449-456
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    • 2014
  • Life table analysis and temperature-dependent development experiments were conducted to understand the biological characteristics of the cabbage aphid, Brevicoryne brassicae (Linnaeus) on detached Tah Tsai Chinese cabbage (Brassica campestris var. narinosa) leaves at seven constant temperatures (15, 18, 21, 24, 27, 30 and $33{\pm}1^{\circ}C$; $65{\pm}5%$ RH; 16L:8D). Mortality was lowest at $24^{\circ}C$ with 18% and 0% at $1^{st}{\sim}2^{nd}$ and $3^{rd}{\sim}4^{th}$ nymphal stages, respectively. The developmental period of $1^{st}{\sim}2^{nd}$ nymphal stage was 8.4 days at $18^{\circ}C$, and it decreased with increasing temperature. The developmental period of the $3^{rd}{\sim}4^{th}$ nymphal stage was 6.7 days at $18^{\circ}C$. The lower threshold temperature calculated using a linear model was $7.8^{\circ}C$, and the effective accumulative temperature was 120.1DD. Adult longevity was 14.9 days at $21^{\circ}C$, and total fecundity was observed 58.5 at $24^{\circ}C$. According to the life table, the net reproduction rate was 47.5 at $24^{\circ}C$, and the intrinsic rate of increase and the finite rate of increase were 0.36 and 1.43, respectively, at $27^{\circ}C$. The doubling time was 1.95d at $27^{\circ}C$, and mean generation time was 7.43d at $30^{\circ}C$.

The effects of blocking the oxygen in the air during the polymerization of sealant (광중합 시 공기 중 산소의 차단이 치면열구전색제의 중합에 미치는 영향)

  • Oh, You-Hyang;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.3
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    • pp.365-376
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    • 2006
  • The purpose of this study was to evaluate the efficacy of blocking the oxygen in the air during the polymerization of sealant. All curing were performed with various light curing units under the application of oxygen gel barrier, stream of nitrogen and carbon dioxide gas for inhibition of oxygen diffusion into sealant surface. The results of present study can be summarized as follows : 1. The amount of eluted TEGDMA form the specimens cured with all the three different light units in the stream of $N_2$ and $CO_2$ gas and application of Oxygen gel barrier($DeOx^{(R)}$) were significantly lower than in the room-air atmosphere (Control) (p<0.05). 2. In the $DeOx^{(R)}$ application, the amount of eluted TEGDMA the specimen cured with PAC light for 10seconds was less than that cured in the stream of $N_2$ and $CO_2$ atmospheric conditions (p<0.05) 3. In the LED using 10 or 20sec irradiation times under the stream of $N_2$ and $CO_2$, the eluted TEGDMA showed to be no statistically significant difference (p>0.05). 4. The microhardness from the specimens cured with all the three different light units under each treated conditions were significantly higher than in the room-air atmosphere (p<0.05). 5. The surface treatment by $DeOx^{(R)}$, $N_2$ and $CO_2$ reduces the thickness of oxygen inhibited layer by sp proximately 49% of the untreated control value.

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Characteristics of Maize Line Treated with Mutagen of γ-Ray at M1 Generation (돌연변이원(突然變異原) γ-선(線)을 처리(處理)한 M1 세대(世代) 옥수수 계통(系統)의 특성(特性))

  • Lee, Hee Bong
    • Korean Journal of Agricultural Science
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    • v.22 no.2
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    • pp.156-162
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    • 1995
  • This study was carried out to select the useful mutants among 20 lines treated with mutagen, gamma ray. These materials were treated with 15, 20, 25 and 30Krads, respectively and sowed at experimental field of College of Agriculture on 27th May, 1995. Items surveyed were germination rate and loss of chlorophyll at seedling stages, abnormal reproductive organs, discordance of flowering times and stem and ear height at ripening stages. The germination rate of lines treated with 15 Krad was different. The germination rate of the IK1, IK2 and Sinki lines were high, while the IK3, A632 and FR140 lines were very low. At same dose level, the germination rate of both IK1/H26 and Puyo synthetic variety were different; the germination rate of the IK1/H26 was high as above 70% at all treatments, while that of the Puyo synthetic variety was very poor at above 20 Krad. Generally stem adn ear height of the IK1/H26 were gradually decreased as the dose of mutagen increased, but there was no difference in stem and ear height of the IK1/H26 when this line was treated with 20Krad. The stem and ear height of lines treated with 15 Krad were shown to be decreased than those of check with non-treatment. The number of tiller of tillering lines were not greatly affected by ${\gamma}$-ray treatment.

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The Effect of the Anthracnose Resistant Chili Pepper (PepEST gene) on the Non-target Insects, Green Peach Aphids (Myzus persicae Sulzer, Homoptera) (유전자 변형 탄저병 저항성 고추(PepEST gene)가 비표적 곤충인 복숭아혹진딧물(Myzus persicae Sulzer)에 미치는 영향)

  • Park, Ji-Eun;Yi, Hoon-Bok;Kim, Chang-Gi;Jeong, Soon-Chun;Yoon, Won-Kee;Park, Kee-Woong;Lee, Bum-Kyu;Kim, Hwan-Mook
    • Korean journal of applied entomology
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    • v.46 no.3
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    • pp.343-348
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    • 2007
  • To assess the environmental risks of transgenic chili pepper with PepEST gene on non-target organisms before it exposes to the agro-ecosystem environments, we conducted the three sets of green peach aphids (Myzus persicas S.) life table experiment under laboratory conditions (Temp. $25^{\circ}C$, R.H. 50-70%, Photoperiod L16 : D8) in series during 2005-2006. We measured the net reproductive rate ($R_0)^*$, the intrinsic rate of increase ($r_m$), the mean generation time ($T_c$), $fecundity^*$, life span, and reproduction period between non-transgenic chili peppers and transgenic chili peppers, respectively. The life span of green peach aphids from three sets was 31, 27, 25 days, and the period of life span was similar to the general average length of green peach aphids, 25-29 days. Although the first reproduction of transgenic pepper was similar to the non transgenic pepper (P>0.05), the fecundity and the net reproductive rate ($R_o$) by using Jackknife method of transgenic pepper were lower than those of non transgenic pepper (P<0.05). Conclusively, we observed the adverse effect from our results but we should execute further experiments to confirm the results at the fields with the similar way.

ABRASION AND CHEMICAL DEGRADATION OF LIGHT-CURED COMPOSITE RESIN FOR UPDATED RESIN DEVELOPMENT (차세대 레진개발을 위한 광중합형 복합레진의 마모와 화학적 분해)

  • Yang, Kyu-Ho;Choi, Nam-Ki;Yook, Geun-Young
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.4
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    • pp.685-695
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    • 2004
  • The aim of this study was to evaluate the resistance to degradation and to compare the wear resistance characteristics of four composite resins in an alkaline solution. The resistance to degradation was evaluated on the basis of mass loss(%), degradation depth(${\mu}m$), Si loss(ppm) and wear depth. The brands studied were Heliomolar flow, Filtek supreme, Point4, Tetric flow. The results were as follows: 1. The sequence of the mass loss was in descending order by Heliomolar flow, Filtek supreme, Point4, Tetric flow. There was significant differences among the materials except Heliomolar flow and Filtek supreme. 2. The sequence of the degree of degradation layer depth was in descending order by Filtek supreme, Heliomolar flow, Tetric flow, Point4. There were significant differences among the materials except Heliomolar flow and Tetric flow. 3. The sequence of Si loss was in descending order by Filtek supreme, Heliomolar flow, Point4, Tetric flow. There were significant differences among the materials except Point 4 and Tetric flow. 4. The sequence of maximum wear depth was in descending order by Heliomolar flow, Point4, Fillet supreme, Tetric flow and there was increasing wear depth on soaking in 0.1N NaOH solution. 5. When observed with SEM, destruction of bonding between matrix and filler was observed and when observed with CLSM, the depth of degradation layer of specimen surface was observed. There results indicate that wear and hydrolytic degradation could be considered to be evaluation factors of composite resins.

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WEAR AND CHEMICAL DEGRADATION OF ESTHETIC RESTORATIVE MATERIALS (차세대 레진 개발을 위한 광중합형 복합레진의 화학적 분해와 마모에 관한 연구)

  • Yang, Kyu-Ho;Choi, Eun-Young;Choi, Nam-Ki;Kim, Seon-Mi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.3
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    • pp.557-568
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    • 2005
  • The aim of this study was to evaluate the resistance to degradation and to compare the wear resistance characteristics of four esthetic restorative materials in an alkaline solution(0.1N NaOH). The brands studied were MetafilCX(Sun medical, Japan) Solitaire 2(Heraeus Kulzer, USA), Composan LCM(Promedica, Germany), DenFil(Vericom, Korea). The results were as follows: 1. The mass loss of each brand was 0.74~7.94% and highest value in Metafil($7.94{\pm}0.39%$). 2. The sequence of the degree of degradation layer depth was in descending order by Metafil, Solitaire 2, DenFil, Composan LCM. There were significant differences between Metafil and the others(p<0.05). 3. The sequence of the Si loss was in descending order by Metafil, Solitaire 2, Composan LCM, DenFil. There were significant differences among the materials(p<0.05). 4. On SEM, destruction of bonding between matrix and filler and on CLSM, the depth of degradation layer of specimen surface was observed. 5. The sequence of maximum wear depth was in descending order by Metafil, Solitaire 2, Composan LCM and DenFil. There were significant differences among the materials(p<0.05). 6. The correlation coefficient between Si loss and degradation layer depth (r=0.491, p<0.05) and Vicker's hardness number and maximum wear depth (r=-0.942, p<0.05) were relatively high. These results indicate that hydrolytic degradation and wear may consider as a evaluation factors of composite resins.

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Susceptibility tests of oral antibiotics including cefixime against Escherichia coli, isolated from pediatric patients with community acquired urinary tract infections (소아 원외 요로감염 환아에서 분리된 E. coli에 대한 cefixime을 포함한 경구 항생제의 감수성 연구)

  • Lee, Soo Young;Lee, Jung Hyun;Kim, Jong Hyun;Hur, Jae Kyun;Kim, Sun Mi;Ma, Sang Hyuk;Kang, Jin Han
    • Clinical and Experimental Pediatrics
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    • v.49 no.7
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    • pp.777-783
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    • 2006
  • Purpose : Urinary tract infection(UTI) is one of the most frequent infections in children. E. coli is the most frequent etiological micropathogen in pediatric community UTI, and E. coli has developed resistance to many antibiotics, highlighting the need for regular surveys of this organism resistant patterns in the community. The aim of this study was to determine the oral antibiotic susceptibility patterns of E. coli, isolated from pediatric patients with uncomplicated community acquired UTI. Methods : E. coli isolates, obtained from pediatric patients with uncomplicated community acquired UTI between October in 2004 to September in 2005. And minimal inhibitory concentrations(MICs) of oral aminopenicillins and beta-lactamase inhibnitors(ampicillin, amoxacillin, ampicillin-sulbactam), oral cephalosporins(cefaclor, cefixime) and sulfa drug(trimethoprime-sulfamethoxazole) were performed according to the National Committee for Clinical Laboratory Standards(NCCLS) guide line. Results : Total 211 organisms were isolated from pediatric out-patients with community UTI. E. coli was the most common organism(89 percent), followed by E. fecalis, Proteus species, S. aureus, M. morganii, and P. aeruginosa. The resistant rates of aminopenicillins and beta-lactamase inhibitors, cefaclor and sulfa drug to E. coli were very high. But, the resistant rate of cefixime was markedly low, and ESBL strains were isolated with small rates. Conclusion : Our study results suggest that aminopenicillins, cefaclor and sulfa drug may not be useful as first line empirical antibiotics to treat pediatric patients with community UTI in Korea. But, 3rd generation cephalosporin such as cefixime can be used as effective second line antibiotics after primary treatment failure, also may be useful as an empirical first line antibiotic. Finally, we conclude that a continuous surveillance study to monitor susceptibility patterns of E. coli in community UTI will be needed for the standard guide lines of empirical oral antibiotic treatment.

A Study on the Insurance Contribution and Health Care Utilization of the Regional Medical Insurance Scheme (1개 군지역 의료보험제도에서의 보험료 부담수준별 병.의원 의료이용에 관한 연구)

  • Lee, Sang-Il;Choi, Hyun-Rim;Ahn, Hyeong-Sik;Kim, Yong-Ik;Shin, Young-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.22 no.4 s.28
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    • pp.578-590
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    • 1989
  • This study was conducted to assess the equity in the regional insurance scheme through analysis of the computerized data from one regional insurance society and National Federation of Medical Insurance. We analysed the insurance contribution and benefit by the classes based on total and income-related contribution per household. The major findings of this study are as follows : 1. The average proportion of income-related contribution among the total was 39.2% and the upper classes show higher proportion of the income-related contribution. 2. The upper classes show higher health care utilization rate than the lower classes. It suggests that the lower classes have relatively large unmet medical needs. 3. The analysis through the Lorenz curve reveals that there exists transference of contributions from the upper to lower classes. But the cumulative percentage of insurance benefit is smaller than that of the number of the insured. It implies that regional medical insurance scheme in Korea has still some inequity in the context of social security principles.

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Comparison of gut microbial diversity of breast-fed and formula-fed infants (모유수유와 분유수유에 따른 영아 장내 미생물 군집의 특징)

  • Kim, Kyeong Soon;Shin, Jung;Sim, JiSoo;Yeon, SuJi;Lee, Pyeong An;Chung, Moon Gyu
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.268-273
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    • 2019
  • The intestinal microbiomes vary according to the factors such environment, age and diet. The purpose of this study was to compare the gut microbial diversity between Korean infants receiving breast-fed milk and formula-fed milk. We analyzed microbial communities in stool samples collected from 80 Korean infants using next generation sequencing. Phylum level analysis revealed that microbial communities in both breast-fed infants group (BIG) was dominated by Actinobacteria ($74.22{\pm}3.48%$). Interestingly, the phylum Actinobacteria was dominant in formula-fed infants group A (FIG-A) at $73.46{\pm}4.12%$, but the proportions of phylum Actinobacteria were lower in formulafed infants group B and C (FIG-B and FIG-C) at $66.52{\pm}5.80%$ and $68.88{\pm}4.33%$. The most abundant genus in the BIG, FIG-A, FIG-B, and FIG-C was Bifidobacterium, comprising $73.09{\pm}2.31%$, $72.25{\pm}4.93%$, $63.81{\pm}6.05%$, and $67.42{\pm}5.36%$ of the total bacteria. Furthermore, the dominant bifidobacterial species detected in BIG and FIG-A was Bifidobacterium longum at $68.77{\pm}6.07%$ and $66.85{\pm}4.99%$ of the total bacteria. In contrast, the proportions of B. longum of FIG-B and FIG-C were $58.94{\pm}6.20%$ and $61.86{\pm}5.31%$ of the total bacteria. FIG-A showed a community similar to BIG, which may be due to the inclusion of galactooligosaccharide, galactosyllactose, synergy-oligosaccharide, bifidooligo and improvement material of gut microbiota contained in formula-milk. We conclude that 5-Bifidus factor contained in milk powder promotes the growth of Bifidobacterium genus in the intestines.