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Changes in Abscisic Acid and Gibberellin Levels during Stratification in Panax ginseng Roots (인삼근의 휴면타파과정에 있어서 Abscisic acid 함량 및 Gibberellin 활성의 변화)

  • Choi, Sun-Young;Lee, Kang-Soo;Ryu, Jeom-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.1
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    • pp.7-13
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    • 1989
  • The present study was carried out to get the basic information for clarifying physiological mechanism of breaking dormancy and sprouting in Panax ginseng roots. Changes in Abscisic acid (ABA) content and Gibberellin (GA) activity were investigated in one-year-old root during stratification at 4$^{\circ}C$. 15$^{\circ}C$. and 15$^{\circ}C$ after 60day-treatment at 4$^{\circ}C$. Sprouting rate at 15$^{\circ}C$ was 35% in 30days storage at 4$^{\circ}C$ and 100% in longer than 60days, but there was no sprout in both the constant treatment at 4$^{\circ}C$ or 15$^{\circ}C$ regardless of the treatment period. The longer the period of low temperature treatment. number of days to the first and 50% sprouting was shortened, and number of days to 50% from first sprouting was also shortened. ABA content in the upper part of root(contained bud) was gradually increased at both 4$^{\circ}C$ and 15$^{\circ}C$ as the treatment period was extended. and the degree of increase was higher at 15$^{\circ}C$. In the lower part. it showed a slight increase at 15$^{\circ}C$. while showed little change at 4$^{\circ}C$ throughout the treatment period. In the 15$^{\circ}C$ treatment after 60days at 4$^{\circ}C$, it was greatly increased in the upper part. while rather slightly decreased in the lower part of root. GA activity in the upper part was gradually decreased at both 4$^{\circ}C$ and 15$^{\circ}C$, and the degree of decrease was higher at 15$^{\circ}C$. In the lower part. it was similar tendency to those in the upper part. In the 15$^{\circ}C$ treatment after 60days at 4$^{\circ}C$. it was remarkably increased in both the upper and lower part. The increase was great in the low Rf region, while the decrease appeared relatively in the high Rf region compared to those of 60day-treatment at 4$^{\circ}C$. The above results indicated that the breaking dormancy and sprouting of bud were closely associated with the degree of GA activities in response to temperature condition .during stratification rather than the direct effect associated with the changes in ABA content.

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Validation of the physical activity classification table for Korean youth and assessment of total energy expenditure, estimated energy requirement and physical activity in Korean children and adolescents (한국 소아청소년을 위한 신체활동분류표의 타당도 평가 및 이를 이용한 일일 총에너지소비량, 에너지필요추정량과 신체활동 평가)

  • Ji-Yeon Gwak;Myung-Hee Kim;Jonghoon Park;Kazuko Ishikawa-Takata;Eun-Kyung Kim
    • Journal of Nutrition and Health
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    • v.56 no.1
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    • pp.35-53
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    • 2023
  • Purpose: The purpose of the first part of this study was to evaluate the validity of the physical activity classification table for youth (Youth-PACT). The second part of this study was aimed at comparing the estimated energy requirement (EER) with the total energy expenditure (TEE) and evaluating the physical activity patterns of Korean children and adolescents. Methods: The subjects of the first part of the study were 17 children aged 10 to 12 years, and their total energy expenditure (TEEDLW) was measured using the double labeled water (DLW) method. A total of 166 children and adolescents aged 6-18 years participated in the second part of this study. Their resting energy expenditure (REE) was measured using indirect calorimetry and the TEEYouth-PACT and physical activity level were calculated by applying the Youth-PACT to the physical activity diary prepared by the subjects. Results: In the first part of this study, there were no significant differences between the TEEDLW and the TEEYouth-PACT. The TEEYouth-PACT accurately predicted TEEDLW in 37.5% of the subjects. In the second part of the study, the rates at which EER accurately predicted TEE YouthPACT and overestimated TEE Youth-PACT were 29.6% and 47.3%, respectively. The time spent based on intensity of physical activity and the physical activity categories which were obtained using Youth-PACT showed different patterns according to sex and age group. Age showed significant positive correlations with REE, TEE, and the time spent in sedentary behavior, but age was significantly negatively correlated with REE/body weight, TEE/body weight, and the time spent in low-intensity and high-intensity activities. Conclusion: The results of this study showed that the Youth-PACT can be used to evaluate the TEE and PAL of children and adolescents. However, further studies are needed to validate the TEEYouth-PACT and to set the EER for children and adolescents.

The effect of RGB LED lights on oyster mushroom (Pleurotus spp.) fruit-body characteristics (RGB LED 광원이 느타리류의 자실체 특성에 미치는 효과)

  • Jae-San Ryu;KyeongSook Na;Jeong-Han Kim;Jeong Woo Lee;Hee-Min Gwon
    • Journal of Mushroom
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    • v.21 no.3
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    • pp.132-139
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    • 2023
  • Light plays an important role in fruit-body development and morphology during Pleurotus spp. cultivation. To understand the effects of light color on fruit-body properties, we evaluated the fruit-body characteristics of Pleurotus spp. Varieties cultivated under blue, red, and purple LED light sources. The main results are as follows: The overall fruit-body shape showed differences depending on the color of the LED light. The fruit-bodies of mushroom cultivated under blue and purple light were generally similar to the mushroom shapes typically produced, while those of mushroom cultivated under green light were abnormally shaped, probably due to the absence of effective light source. The average cap lightness of mushrooms cultivated under blue, green, and purple LED lights was 57.0, 57.4, and 59.4, respectively. The average cap lightness of all varieties except Wonhyeong1ho and Hwang-geumsantari cultivated under the three LED light sources were statistically significantly different (P<0.05). The cap redness varied significantly depending on the LED lighting and variety. Only Gonji7hoM, the cap color mutant of Gonji7ho, showed negative cap redness values under all three LED light sources. Among the eight varieties excluding Gonji7ho, the highest cap redness was observed when cultivated under the blue LED. The average harvest weight of the varieties cultivated under purple, blue, and green LED light were 68.0, 58.3, and 50.1 g, respectively. The yield of Gonji7ho, the mushroom variety with the highest yield, cultivated under blue, green, and purple LED light were 92.8, 77.1, and 98.6 g, respectively. The earliness when grown under the purple, blue, and green LED lights were 5.3, 5.8, and 5.8 days, respectively. Among the varieties, six, three, and two cultivars showed the shortest earliness under the purple, green, and blue LED, respectively. The fruit-body lengths were 66.4, 51.8, and 46.8 mm when cultivated under green, purple, and blue lights, respectively. These results are expected to serve as a foundation for producing mushrooms with traits demanded in the market.

Development of cordycepin fortified meat production in Hanwoo steers I. Determination of the chemical composition and safety of Cordyceps militaris from grain or pupae and Paecilomyces tenuipes (Cordycepin 강화 한우고기 생산에 관한 연구 I. 곡립기주 동충하초 균사체의 화학적 성분 및 안전성 조사)

  • Kim, W.Y.;Lee, S.H.;Kim, D.H.;Lee, J.H.;Nho, W.G.;Hwang, J.H.;Yeo, J.M.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.11 no.1
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    • pp.35-51
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    • 2009
  • Three different Cordyceps militaris(Paecilomyces tenuipes and Cordyceps militaris from grain or pupae) were analysed for their chemical composition, fatty acids composition and the concentration of amino acids, cordycepin and heavy metal to obtain basic information on them as feed supplements for livestock. The crude protein content of C. militaris from grain and pupae was 76.16 and 75.45%, respectively, being higher than that of P. tenuipes(57.21%). The concentration of linoleic acid was much higher but that of linolenic acid was much lower for C. militaris from grain than for the others. The cordycepin content was significantly higher for C. militaris from grain than for that from pupae (1.64% vs 0.68% on a DM basis). But cordycepin was not detected in P. tenuipes. Heavy metal contents(Cr, Pb, Cd, Hg) for all C. militaris were below the allowance levels recommended by Ministry of Agriculture and Forestry in Republic of Korea.

Clinical Study of The Motricity Index and Symptoms and signs in Acute Ischemic Stroke Patients on East-West Collaborative Treatment : A Pilot Study (한·양방 병행치료를 시행한 급성기 뇌경색 환자의 운동 기능과 임상 증상 및 증후의 호전도에 관한 연구 : 예비연구)

  • Kim, Min-kyung;Shim, So-ra;Kim, Soo-kyung;Kim, Na-hee;Cho, Seung-yeon;Park, Joo-young;Park, Seong-uk;Jung, Woo-sang;Moon, Sang-kwan;Park, Jung-mi;Cho, Ki-ho;Kim, Young-suk;Bae, Hyung-sup;Ko, Chang-nam
    • The Journal of the Society of Stroke on Korean Medicine
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    • v.12 no.1
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    • pp.41-49
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    • 2011
  • Objective : Stroke is the most common disabling neurological disease of adult life. And some part of the clinical symptoms and signs are correlated to the post stroke complication. This study was to analyze the therapeutic effect of the East-West collaborative treatment, especially on the motor power and the clinical symptoms and signs improvement of ischemic stroke patients. Method : For 2 weeks as possible, we observed the patients with acute ischemic stroke receiving East-West collaborative treatment. We assessed the patients using Motricity Index (MI) and the changes of clinical symptoms and signs three times at seven-day intervals. At the end of the study, we compared the results. Result : Motricity Index of patients changed from 71.55 to 76.03 (upper limb) and 73.05 to 79.42 (lower limb), respectively. Some items of clinical symptoms and signs showed the statistically significant result. Especially, the urination and defecation symptoms improved obviously. Conclusion : The East-West collaborative treatment has an effect on motor power and clinical symptoms and signs improvement of acute ischemic stroke patients.

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Clinical Observation on East-West Integration Treatment in Stroke and Brain Disease (중풍 뇌 질환의 한 방향 협진에 관한 임상적 고찰)

  • Shin, Ae-sook;Lee, ln-whan;Kim, Na-hee;Kim, Hye-rni;Kim, Min-kyung;Sim, So-ra;Cho, Seung-yeon;Park, Seong-uk;Park, Jung-mi;Bae, Hyung-sup;Ko, Chang-nam
    • The Journal of the Society of Stroke on Korean Medicine
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    • v.11 no.1
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    • pp.9-17
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    • 2010
  • Objectives : This study was aimed to evaluate the current status of East-West integrated treatment in stroke and brain disease and to discuss further plans. Methods : The medical records of patients who visited the stroke and brain disease center at Kyung Hee University, East-West Neo Medical Center from May 2006 to August 2010 were evaluated. The general characteristics of patients who underwent integrated treatment, trend in the number of cross referrals were initially evaluated. Later major disorders, the reasons of referrals and the number of visits in outpatients were analyzed. Results : 1. 3496 patients were referred from the eastern medical hospital to the western medical hospital and 2440 patients from the western medical hospital to the eastern medical hospital. The number of patients reached a peak alter the opening of the hospital and has decreased from then on. Referrals of female patients were more than those of male patients and patients over 50 years old were the most. 2. Admitted patients with stroke of chronic stage were most commonly referred from the eastern medical hospital to the western medical hospital and cerebral infarction was most common from the western medical hospital to the eastern medical hospital. Among the outpatients cerebral infarction topped from east to west, and stroke of chronic stage from west to east. 3. 36.6% of the patients from east to west received integrated treatment more than 3 times and 28.6% from west to east. Headache was the second most common reason to be referred from west to east and 36.7% of patients didn't continue to have either of the treatment and 30.3% received eastern treatment only, Conclusions: According to this study, chronic stroke management was successfully performed in the outpatient clinic in the form of East-West integration treatment. Further research on other diseases such as headache is recommended.

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The factors to impact on the satisfaction of field practice among dental hygiene students (일부 치위생(학)과 학생들의 현장임상실습 만족도의 영향을 미치는 요인)

  • Sim, Seon-Ju;Baek, Ha-Oung;Um, Jung-Sun;Jung, Ha-Yan;Ji, Hey Mi;Hwang, Si-Nae;Han, Ji-Hey
    • Journal of Korean Academy of Dental Administration
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    • v.5 no.1
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    • pp.22-30
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    • 2017
  • Objective: The purpose is to study the factors to impact on the satisfaction of field practice among dental hygiene students and to provide the basic data for the quality improvement of the field practices for dental hygiene students. Methods: A self-reported questionnaires were filled out by 108 dental hygiene students in C city. Questionnaires were composed with the general characteristics, satisfaction to major, and satisfaction to field clinical practice. The data was analyzed by chi-square statistics using SPSS 20.0 program. Results: The higher satisfaction to communication with professor or seniors in department was associated with the higher satisfaction to field clinical practice (p<0.05). The higher satisfaction to field clinical practice hours per week was associated with the higher satisfaction to field clinical practice (p<0.05). The higher interest to major subjects and the satisfaction to the laboratory environment were associated with the higher satisfaction to field clinical practice (p<0.05). Conclusions: The higher satisfaction to major was associated with the higher satisfaction to field clinical practice. Therefore, the systematic curriculum development is needed to improve the satisfaction with major and clinical practice.

Effect of storage condition of resin cement on shear bond strength of the orthodontic bracket (레진시멘트의 보관 조건이 치열교정용 브라켓의 전단접착강도에 미치는 영향)

  • Seul-Gi, Yi;Jin-Woo, Kim;Se-Hee, Park;Yoon, Lee;Eung-Hyun, Kim;Kyung-Mo, Cho
    • Journal of Dental Rehabilitation and Applied Science
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    • v.38 no.4
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    • pp.189-195
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    • 2022
  • Purpose: For orthodontic bracket bonding, light curing resin cement is widely used because the process is convenient, and it can be polymerized at the desired time. This study compared the difference of bonding strength of orthodontic resin cement according to storage condition. Materials and Methods: After acid etching the bovine enamel surface with 37% phosphoric acid, 15 orthodontic brackets for mandible incisors were bonded with Ortho Connect and Orthomite LC according to following three conditions; 1) Immediate after 4℃ refrigeration for 3 months (IR), 2) One day room temperature after 4℃ refrigeration for 3 months (OR), 3) Room temperature for 3 months (RT). The shear bond strength was measured with a universal material tester and failure pattern of the specimen was observed. Two-way ANOVA and One-way ANOVA were used at the 95% significance level. Results: Ortho Connect that was applied immediately after refrigeration showed the maximum shear bond strength. Orthomite that was applied immediately after refrigeration showed the lowest shear bond strength, and the group stored at room temperature for three months showed the highest shear bond strength, and the difference between the two groups was significant. Conclusion: Ortho Connect can be used without worrying about bond strength even if it is used immediately after refrigeration, but Orthomite should be kept at room temperature sufficiently after refrigeration.

Investigation on Pesticide Residues in Agricultural Products in Domestic Markets Using LC-MS/MS and GC-MS/MS (LC-MS/MS 및 GC-MS/MS를 이용한 국내 유통 농산물 중 잔류농약 실태조사)

  • Ji-Yeon Bae;Da-Young Yun;Nam Suk Kang;Won Jo Choe;Yong-Hyeon Jeong;Gui Hyun Jang;Guiim Moon
    • Journal of Food Hygiene and Safety
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    • v.38 no.3
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    • pp.131-139
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    • 2023
  • In this study, we investigated pesticide residue levels in 535 domestically distributed agricultural products in South Korea using multi-residue analysis. Agricultural products from 13 regions, including Seoul, were pretreated using QuEChERS and d-SPE, and subsequently analyzed using LC-MS/MS and GC-MS/MS. Residual pesticides were detected in 288 (53.8%) out of the 535 samples, including 40 of apples, 40 of peppers, 33 of mandarins, 31 of peaches, and 144 other commodities. Furthermore, one sample of Korean cabbage exceeded the permitted maximum residue limit (MRL), diniconazole (0.18 mg/kg), detected at about twice the MRL. In total, 91 types of residual pesticides were detected, including fungicides (42), insecticides (48), and a nematicide. The most frequently detected pesticides were dinotefuran (91), carbendazim (75), tebuconazole (61), and pyraclostrobin (59). Our results showed that continuous monitoring of agricultural products is necessary.

Autonomic Nervous Response of Female College Students with Type D Personality during an Acute Stress Task: Heart Rate Variability (Type D 성격 여대생의 급성 스트레스에 따른 자율신경계 반응 : 심박률 변동성을 중심으로)

  • Ko, Seon-Young;Kim, Myung-Sun
    • Korean Journal of Health Psychology
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    • v.14 no.2
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    • pp.277-292
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    • 2009
  • This study investigated the responses of the autonomic nervous system of individuals with Type D personality during an acute stressful situation. Twenty-three female students of Type D personality and 23 female students with non-Type D personality. Stroop Color-Word Task was used to induce a stressful situation, heart rate variability (HRV) was used to measure the responses of the autonomic nervous system during the baseline, acute stress, recovery periods. To analyze the data, the repeated measures analysis of variance was used to compare the autonomic nervous system of the Type D group to that of the non-Type D group. Regression analysis is used to determine if the Type D scale and stress vulnerability predicted the activities of the autonomic nervous system during the baseline period. The results of this study demonstrated that the Type D group's normalized low frequency (LF norm) and ratio of low frequency to high frequency (LF/HF ratio) were higher than those for the non-Type D group, while its normalized high frequency (HF norm) was lower than that for the non-Type D group in all three periods. There were no statistically significant differences among the three periods in terms of LF norm, HF norm, and LF/HF ratio in the Type D group. The study demonstrated that the total scores of the Type DS-14 and scores of social inhibition and negative affect were independent predictors of LF norm and HF norm during the baseline. The Type D group showed increased activation of the sympathetic nervous system and/or decreased activation of the parasympathetic nervous system. These results support the hypothesis that the Type D personality is vulnerable to the stress. Also, the highly activated sympathetic and/or lowly activated parasympathetic nervous systems, which were observed in the Type D group during the baseline, indicated that the Type D individual is susceptible to psychosomatic disorders.