• Journal of Life Science
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• v.30 no.10
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• pp.896-904
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• 2020

The purpose of this study was to investigate the probiotic characteristics and immune activities of selected lactic acid bacterial (LAB) strains as feed additives in livestock. 301 LAB strains isolated from traditional fermented foods were first assessed for their antibacterial activity potential. Of the 301 isolates, five showed antibacterial activity against five livestock pathogens (Esherichia coli KCCM11234, Listeria monocytogens KCTC3710, Salmonella Typhimurium KCTC1926, Staphylococcus aureus KCCM11593, and Shigella flexneri KCTC2517). The probiotic characteristics of the five selected strains were also investigated by antioxidative activity, hemolysis, bile salt hydrolase, acid resistance and bile tolerance. The SRCM102607 strain was found to have superior probiotic properties and was selected for further experimentation. 16S rRNA gene sequencing showed that SRCM102607 is Pediococcus acidilactici, which was labeled as P. acidilactici SRCM102607 (KCCM 12246P). The survival characteristics of P. acidilactici SRCM102607 in artificial gastrointestinal conditions were assessed under exposed acidic (pH 2.0) and bile (0.5% and 1.0%) conditions. P. acidilactici SRCM102607 was also confirmed to have resistance to various antibiotics, including amikacin, gentamicin, vancomycin, and etc. The TNF-α production by P. acidilactici SRCM102607 was 171.86±4.00 ng/ml. These results show that P. acidilactici RCM102607 has excellent potential for use as a probiotic livestock feed additive.

• Microbiology and Biotechnology Letters
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• v.16 no.2
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• pp.150-155
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• 1988

In order to produce L-lysine from cellulosic substrates by the intergeneric protoplast fusion between cellulolytic bacteria, Cellulomonas flavigena KFCC31221 and amino acid producing bacteria, Brevibacterium flavum ATCC14067, Corynebacteriurn glutamicum ATCC13032, conditions for protoplast formation and regeneration of these strains were investigated. After the strains were mutated with 500$\mu\textrm{g}$/$m\ell$ N-methyl-N'-nitro N-nitrosoguanidine for 30 min and the mutants were enriched by treating 300$\mu\textrm{g}$/$m\ell$ penicillin-G for 2 hrs, B. flavum Hse- Str$^{r}$ , C. glutamicum Met$^{-}$Thr$^{-}$ Rif$^{r}$ and Cellulomonas flavigena Thr$^{-}$Val$^{-}$Kan$^{r}$ were isolated. The rate of protoplast formation ranged from 95 to 98% when strains were treated at the concentration of 500$\mu\textrm{g}$/$m\ell$ of lysozyme, pH 6.5, 33$^{\circ}C$, for 6 hrs. in Tris- malate buffer supplemented with 0.4M sucrose as osmotic stabilizer. Approximately 30-33% protoplast was regenerated on the regeneration complete medium(RCM) containing 1.5% agar and 0.5M sodium succinate overlaid with the same medium except 0.7% agar.

• Journal of Korea Water Resources Association
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• v.40 no.2 s.175
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• pp.147-158
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• 2007

This study attempted to determine a suitable hydrologic model for assessing the impact of climate change on water resources, and to assess the accuracy of streamflow scenarios simulated by the selected hydrologic model using the meteorological scenarios of the Seoul National University Regional Climate Model(SNURCM). Comparison of four water balance models and two daily conceptual rainfall-runoff models for the simulation capability of the Daecheong Dam inflow indicated that the abcd model performs the best among the tested water balance models and performs as well as SSARR that is popular as a daily rainfall-runoff model in Korea. Parameters of the abcd model were then estimated for 12 ungauged subbasins of the Geum River by the regionalization method. The model parameters were first calibrated at nine multi-purpose dams and were then regionalized using catchment characteristics for another four multi-purpose dams, which were assumed to be ungauged sites. The model efficiency(ME) coefficients of the simulated inflows for these four dams were at least 87%. The MEs of the hindcasted meteorological rainfall scenarios of the 12 subbasins of the Geum River were more than 60%. Moreover, the ME of the Daecheong Dam inflow simulated by the abcd model using the SNURCM rainfall scenarios was more than 80%. Therefore, this research concluded that the abcd model coupled with the SNU-RCM meteorological scenarios can be used for impact assessment studies of climate change on water resources.

• Journal of the Korean earth science society
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• v.21 no.5
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• pp.541-552
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• 2000

The variations of physical properties due to inflow of seawater by sluice gates operation were observed in Shihwa Lake. The distributions of salinity and temperature were investigated at 11 stations during February, 1997 to July, 1998. The salinity of water mass in Shihwa Lake before gate operation was ranged below 15psu and strong stratification due to inflow of seawater was observed at the depth of 11 m. In July 1997, temperature difference of 10^{\circ}C$ was occurred between the surface and bottom water due to strong solar radiation. During October 1997 to February 1998, inversion of temperature distribution, which the temperature of bottom water was higher than that of surface water, was observed. In July 1997, temperature, salinity, current speed and current direction were investigated by RCM-7 at St.3 for 56 days. When sea water was intruded in Shihwa Lake, the symmetric distribution of temperature and salinity was observed and it seems to be resulted from inflow of seawater with low temperature and high salinity. After January 1998, salinity of Shihwa Lake was increased over 30psu due to continuous gate operation and the stratification was weakened.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.1215-1219
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• 2016

Background: Worldwide, breast cancer is the most common cancer among women and is a leading cause of cancer death. In the present study, we investigated the NQO1 C609T genotypic and allelic distribution in north Indian breast cancer patients. Materials and Methods: The genotypic distribution of the NQ01 C609T polymorphism was assessed in 100 invasive ductal carcinoma (IDC) breast cancer patients and 100 healthy controls using allele specific PCR (AS-PCR). Results: A lower frequency of the CC genotype was found in breast cancer patients (24%) than in the controls. On the other hand, TT genotype frequency was also found to be higher in female healthy controls (32%) than the female breast cancer patients (20%). The frequencies of all three genotypes CC, CT, TT in patients were 24%, 56% and 20% and in healthy controls 50%, 22% and 32% respectively. We did not find any significant correlation between the NQO1 C609T polymorphism and age group, grading, menopausal status and distant metastasis. A less significant association was found between the NQ01 C609T polymorphism and the stage of breast cancer (X2=5.931, P=0.05). Conclusions: The present study shows a strong association between NQO1 C609T polymorphism with the breast cancer risk in the north Indian breast cancer patients so that possible use as a risk factor should be further expel.

• Korean Journal of Ecology and Environment
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• v.44 no.2
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• pp.187-194
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• 2011

This study analyzed the A1B climate change scenario provided by National Institute of Meteorological Research (NIMR), Korea, to investigate potential climate changes in watersheds of 15 multi-purpose dams in South Korea. The A1B climate change scenario is produced by Regional Climate Model (RCM) with 27 km horizontal grid spacings using a one-way nesting technique with Global Climate Model (GCM). Relative to present climate conditions (1971~ 2000), the modeled 10-year averaged daily temperatures at the watersheds of the 15 multi-purpose dams continuously increased to year 2100, whereas precipitation changes were varied regionally (north, central, and south regions of South Korea). At two watersheds located in Gangwon-province (north region), the modeled temporal variations of precipitation rapidly increased in the 2090's after a slow decrease that had occurred since the 2050's. At seven watersheds in the central region, including Gyeongsangbuk-province to Jeollanam-province, the modeled temporal variations of precipitation increase showed 10-year periodic changes. At six watersheds in the south region, the modeled temporal variations of precipitation increased since the 2070's after a rapid decrease in the 2060's. Compared to the climate conditions of the late of 20th century (1971~2000), the number of rainy days and precipitation intensity increased (3% and 6~12%, respectively) in the late 21st century (2071~2100). The frequency of precipitation events tended to increase with precipitation intensity in all regions. The frequency of heavy precipitation events (>50 mm $d^{-1}$) increased with >100% in the north region, 60~100% in the central region, and 20~60% in the south region.

• Journal of the Korean Society for Railway
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• v.19 no.5
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• pp.672-684
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• 2016

UIC Codes 714R & 715R recommend the use of line classifications and their usage in maintenance work by employing notional traffic loads. However, the classification has not been applied to local lines and, therefore, a new line classification system based on UIC 714R has been proposed in this study. For this, various classification models of UIC, Germany, and UK have been studied first and equivalent traffic loads based on Korail's report, as well as on train timetables, have been derived. The results of the classifications have been compared with those of major European countries and it has been shown that the proposed classification is equivalent to the average value in the European cases. The line classification can be fully utilized during the decision making process of maintenance work and will also be used to model the Reliability Centered Maintenance (RCM) in the future.

• The Journal of Korean Society for Radiation Therapy
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• v.23 no.1
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• pp.13-19
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• 2011

Purpose: It's essential to minimize the tumor motion and identify the exact location of the lesions to achieve the improvement in radiation therapy efficiency during SBRT. In this study, we made the established compression belt to reduce respiratory motion and evaluated the usefulness of clinical application in SBRT. Materials and Methods: We analyzed the merits and demerits of the established compression belt to reduce the respiratory motion and improved the reproducibility and precision in use. To evaluate the usefulness of improved compression belt for respiratory motion reduction in SBRT, firstly, we reviewed the spiral CT images acquired in inspiration and expiration states of 8 lung cancer cases, respectively, and analyzed the three dimensional tumor motion related to respiration. To evaluate isodose distribution, secondly, we also made the special phantom using EBT2 film (Gafchronic, ISP, USA) and we prepared the robot (Cartesian Robot-2 Axis, FARARCM4H, Samsung Mechatronics, Korea) to reproduce three dimensional tumor motion. And analysis was made for isodose curves and two dimensional isodose profiles with reproducibility of respiratory motion on the basis of CT images. Results: A respiratory motion reduction compression belt (Velcro type) that has convenient use and good reproducibility was developed. The moving differences of three dimensional tumor motion of lung cancer cases analyzed by CT images were mean 3.2 mm, 4.3 mm and 13 mm each in LR, AP and CC directions. The result of characteristic change in dose distribution using the phantom and rectangular coordinates robot showed that the distortion of isodose has great differences, mean length was 4.2 mm; the differences were 8.0% and 16.8% each for cranio-caudal and 8.1% and 10.9% each for left-right directions in underdose below the prescribed dose. Conclusion: In this study, we could develop the convenient and efficient compression belt that can make the organs' motion minimize. With this compression belt, we confirmed that underdose due to respiration can be coped with when CTV-PTV margins of mean 6 mm would be used. And we conclude that the respiratory motion reduction compression belt we developed can be used for clinical effective aids along with the gating system.

• Korean Journal of Food Science and Technology
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• v.21 no.1
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• pp.154-163
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• 1989

This studies were designed to improve the productivity of L-lysine by protoplast fusion and immobilized system of fusants using strains of Brevibacterium flavum ATCC 21528, Brevibacterium lactofermentum ATCC 21086 and Corynebacterium glutamicum 820. Mutants were isolated with concentration method of $300{\mu}g/ml$ penicillin-G after treatment of $250{\mu}g/ml$ N-methyl-N-nitro-N-nitrosoguanidine. B. flavum $37-2(Hos^-,\;Kan^r,\;AEC^r)$, B. lactofermentum $6-2(Ile^-,\;Val^-,\;Str^r,\;AEC^r)$ and C. glutamicum 57-5$(Met^-,\;Thr^-,\;Rif^r,\;AEC^r)$ were isolated from mutants. Protoplasts were induced by being incubated with $500{\mu}g/ml$ lysozyme of lysis solution for 6 hr and the ratio of protoplast formation and regeneration were ranging from 97-99% and 33-37%, respectively. Fusion frequencies of fusants of BBFL 21, BCFG 37 and BCLG 59 were shown in the range from $1.25{\times}10^{-6}\;to\;5.83{\times}10^{-7}$ under the optimum conditions. The fusant BBFL 21 showed the highest productivity of $411.1\;ng/ml{\cdot}hr$ L-lysine in the lysine productivity broth at $30^{\circ}C$ for 72hr. In the immobilization systems, fusant BBFL 21 was employed in various polymer matrices such as sodium alginate, polyacrylamide, agar and ${\alpha}-carrageena$. The immobilization of sodium alginate showed the highest productivity of $413\;ng/ml{\cdot}hr$ L-lysine in the batch system. Continuous fermentation of immobilization system by using tube fermentor was produced the highest productivity $416.7\;ng/ml{\cdot}hr $ L-lysine under optimum condition.

• Journal of Aquaculture
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• v.19 no.4
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• pp.275-280
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• 2006

Effect of the various sources of dietary additives on growth, body composition and shell color of abalone Haliotis discus hannai was investigated for 16 weeks. Forty juvenile abalone averaging 13.5 g were randomly stocked into 21 of 50 L plastic rectangular containers each. Eight kinds of additives were prepared for this study: four commercially available microalgae [Haeatococcus (Hae), Isochrysis galbana (Iso), Shizochytrium (Sch) and Spirulina (Spi)], three crustacean meals [krill meal (KM), shrimp head meal (Shm) and red crab meal (Rcm)], and green tea by-product (Gre). In addition, dry sea tangle (Dst), Laminaria japonica, as a control, was prepared. Casein, dextrin and a mixture corn oil and fish oil was protein, carbohydrate and lipid sources, respectively, in the experimental diets. The 2% each additive was included into the experimental diets. The experimental diets were fed to abalone once a day at the ratio of $1.5{\sim}2.0%$ total biomass of abalone with a little leftover throughout the 16-week feeding trial. Survival of abalone was not significantly (P>0.05) affected by the experimental diets. However, weight gain of abalone fed the all experimental diets containing the various sources of additives was significantly (P<0.05) higher than that of abalone fed the Dst diet. Weight gain of abalone fed the Spi diet was highest and Shi, KM and Iso diets in order. Shell length and the ratio of soft body weight to body weight of abalone was not significantly (P>0.05) affected by the experimental diets. However, shell width of abalone fed the all experimental diets containing the various sources of additives was significantly (P<0.05) higher than that of abalone fed the Dst diet. The shell color of abalone fed the Spi diet was improved the most distinctively and similar to that of natural abalone. Therefore, it can be concluded that the experimental diets with the various sources of additives (microalgae and crustacean meals) was effective to improve growth of abalone and dietary inclusion of Spirulina was most effective to improve shell color of abalone.