• Korean Journal of Food Science and Technology
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• 제39권6호
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• pp.681-687
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• 2007

This study was designed to examine the suitable characteristics of potential probiotic bacteria. Possible probiotic bacteria, including Lactobacillus acidophilus DDS-1, Lb. acidophilus B-3208, Bifidobacterium bifidum KCTC 3357, Lb. plantarum, Leuconostoc mesenteroides ssp. mesenteroides ATCC 8293, and Lactococcus lactis ssp. lactis ATCC 7962 were selected. We then measured their acid and bile tolerances, adhesion properties in the gastrointestinal tract, antimicrobial activity against pathogenic bacteria, and immunomodulation activity. The acid tolerances of Lb. acidophilus DDS-1, Lb. acidophilus B-3208, Lb. plantarum, and Leu. mesenteroides ssp. mesenteroides ATCC 8293, in PBS (pH 2.5) for 2 hr, were high enough that 50% of the inocula survived. The bile tolerances of all bacteria, except Lc. lactis ssp. lactis ATCC 7962, were also observed at a 3% oxgall concentration in MRS broth. The results of the adhesion property assay showed that the total binding affinities of Lb. acidophilus DDS-1, Lb. acidophilus B-3208, and B. bifidum were about three times higher than those of the other bacteria. In testing their antimicrobial activities against pathogens, Lb. acidophilus B-3208, B. bifidum KCTC 3357, and Lb. plantarum inhibited the growth of pathogenic bacteria. For their immunomodulation activity, the cell wall fractions from Lb. acidophilus DDS-1 and Lb. acidophilus B-3208 showed the highest bone marrow cell proliferation activities. However, the cell wall fractions of Lb. acidophilus DDS-1 and B. bifidum, and the cytosol fraction of Lc. lactis ssp. lactis ATCC 7962 showed higher macrophage stimulation activities than those of the other bacteria. Since Lb. acidophilus DDS-1 and Lb. acidophilus B-3208 satisfy the requirements for probiotics, they can be considered suitable probiotic bacteria.

• Tuberculosis and Respiratory Diseases
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• 제43권6호
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• pp.842-851
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• 1996

Background : Rifampicin(RFP) is a key component of the antituberculous shon-course chemotherapy and the RFP-resistance is a marker of multi-drug resistant(MDR) M. tuberculosis. rpoB gene encodes the ${\beta}$-subunit of RNA polymerase of M. tuberculosis which is the target of RFP. Recent reports show that rpoB gene mutations are the cause of RFP resistance of M. tuberculosis and the main mechanism of rpoB gene mutation is point mutation. And PCR-SSCP is a rapid and easy method for detecting point mutations. So we performed PCR-SSCP of rpoB gene of M. tuberculosis and compared the result with traditional RFP sensitivity test. Method : The 27 RFP sensitive M. tuberculosis culture isolates and 25 RFP resistant isolates were evaluated. The RFP sensitivity test was done at the Korean Tuberculosis istitute. The DNA was extracted by bead beater method and was amplified with primers TR-8 and TR-9 in a 20ul PCR reaction containing 0.1ul(luCi) [${\alpha}-^{32}P$] - dCTP. After amplification, SSCP was done using non-denaturaring polyacrylamide gel electrophoresis. Then direct sequencing was done in cases of different eletrophoretic mobility compared with that of H37Rv. In 19 cases, we compared PCR-SSCP results with patient's clinical course and the results of traditional RFP sensitivity test. Results : 1) All 27 RFP sensitive M. tuberculosis isolates showed the same electrophoretic mobility compared with that of H37Rv. And all 25 RFP resistant M. tuberculosis isolates showed different electrophoretic mobility. 2) The mechanism of rpoB gene mutation of M. tuberculosis is mainly point mutation. 3) The PCR-SSCP results correlate well with traditional RFP sensitivity and patient's clinical response to antituberculous treatment. Conclusion: The PCR-SSCP of rpoB gene is a very sensitive and rapid mehod in detecting RFP- resistant M. tuberculosis.

• Korean Journal of Soil Science and Fertilizer
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• 제43권1호
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• pp.83-89
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• 2010

We investigated the growth of cucumber plants, the uptake and use of mineral nutrients, such as $NO_3$-N, $NH_4$-N, $K^+$, $Ca^{+}^{+}$, $Mg^{+}^{+}$ and $Na^+$, absorbed from media solution, and the synthesis and distribution of soluble sugars under nutrient-deficient condition. Difference in plant growth revealed after 20 days of treatment. Nitrate uptake in nutrient-deficient condition was significantly reduced compared with nutrient-normal treatment, and its distribution was primarily in petioles, stem, roots and less in leaves. In contrast, ammonium content was markedly predominated in fast growing organs, and it was significantly different in growing leaves, expanded leaves, and roots under similar growth conditions. $K^+$, lack by deficient nutrient condition, was found in growing leaves. The $Ca^{+}^{+}$ content did not show significant difference between treatments and a substantial portion of $Ca^{+}^{+}$ remained in petioles. The $Mg^{+}^{+}$ content was significantly higher in the leaves of nutrient-normal condition compared with nutrient-deficient condition while significantly lower in stem and roots. The behavior of $Na^+$ in plant was similar to $K^+$ although its content was relatively little. The highest $CO_2$ assimilation was observed in fully expanded leaves of nutrient-normal condition, which was 1.7 times higher compared with nutrient-deficient condition. The instantaneous water use efficiency (A/E) and the A/gsratio, which is an index of leaf intrinsic water use efficiency for individual leaves, was 1.2 and 1.1 times higher, respectively. The total soluble sugar (TSS) contents were highest in leaves followed by petioles, stems and roots, and in younger leaves. The growing leaves contained about 7,200 mg $kg^{-1}$ of TSS in nutrient-normal condition whereas the TSS contents in nutrient-deficient condition were not significantly different between leaves. The $Mg^{+}^{+}$ and $NH_4$- N were positively correlated with the TSS whereas $NO_3$ - N was negatively correlated.

• The Journal of Korean Academy of Prosthodontics
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• 제48권1호
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• pp.16-27
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• 2010

Purpose: The aim of this experimental study is to observe the effect of platelet-rich plasma (PRP) on early bone regeneration of rats both in normal condition and in osteoporosis induced by ovariectomy. Material and methods: Total 40 Sprague-Dawley female rats were divided into 4 groups. A 8-mm-diameter calvarial critical-sized defect (CSD) was made by drilling with trephine at the center of calvaria in cranium of every rat. Every CSD was augmented with an osteoconductive synthetic alloplastic substitute ($MBCP^{TM}$) and PRP as follows. Group A; 10 non-ovariectomized rats grafted with only $MBCP^{TM}$. Group B; 10 non-ovariectomized rats grafted with $MBCP^{TM}$ and PRP. Group C; 10 ovariectomized rats grafted with only $MBCP^{TM}$. Group D; 10 ovariectomized rats grafted with $MBCP^{TM}$ and PRP. At 4 weeks after graft, every rat was sacrificed. And histomorphometric analysis was performed by measuring calcified area of new bone formation within the CSD. Results: Comparing four groups, results were obtained as follows. 1. In non-ovariectomized groups, PRP showed a positive effect somewhat but not significant (P > .05). 2. In ovariectomized groups, PRP showed a positive effect significantly (P < .05). 3. In PRP untreated groups, ovariectomy diminished bone regeneration significantly (P < .05). 4. In PRP treated groups, ovariectomy diminished bone regeneration somewhat but not significant (P > .05). Conclusion: Within the limitation of this study, it can be concluded that PRP in combination with an osteoconductive synthetic alloplastic substitute has an effect on bone regeneration more significantly in ovariectomized osteoporotic rats than in normal rats.

• KSCE Journal of Civil and Environmental Engineering Research
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• 제34권2호
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• pp.515-527
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• 2014

This study conducted experiment for understanding engineering characteristics of domestic sands by examining standard sand and sand from Yokji Island and Nakdong River in terms of confining pressure, $K_0$, over consolidation and relative density factors through triaxial compression test. The test showed that deviator stress by strain positively changed as confining pressure and relative density grow while $K_0$ and over consolidation factors do not directly correlated with it. Angle of internal friction decreases as confining pressure increases which strengthens contact force between particles, and declines as relative density drops, whereas $K_0$ and over consolidation factors hardly affect the results. When it comes to volumetric strain, volume expansion decreases as confining pressure increase due to crushability and rearrangement of particles while $K_0$ and over consolidation shows same movement unconditionally, and relative density appears compressed as it grows at the beginning however it expands as axial strain increases. Modulus of elasticity ($E_{sec}$) by strain has tendency into convergence resulting in initial secant modulus of elasticity ($E_{ini}$) > secant modulus of elasticity($E_{sec}$) > tangent modulus of elasticity ($E_{tan}$). On the other hand, it grows as confining pressure and relative density increase while indicating similar modulus of elasticity ($E_{sec}$) regarding on $K_0$ and over consolidation. Slope of critical line (M) tended to decrease as confining pressure increases, follow same line according to $K_0$, confining pressure and relative density, and increase as relative density grows.

• Journal of Yeungnam Medical Science
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• 제16권2호
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• pp.208-218
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• 1999

Background: Lung cancer-associated hypercalcemia is one of the most disabling and life-threatening paraneoplastic disorders. Humoral hypercalcemia is responsible for most lung cancer-associated hypercalcemia. Patients with hypercalcemia are usually in the advanced stage with obvious bulky tumor and carry a poor prognosis. Materials and Methods: Total 29 patients satisfied the following criteria: histologically proven primary lung cancer, corrected calcium level ${\geq}$ 10.5 mg/dL, and symptoms which could possibly be attributed to hypercalcemia. In this retrospective study, we evaluated the various clinical aspects of hypercalcemia, in relation to cancer stage, histologic cell type, mass size, bone metastasis, performance status, and other possible characteristics. Results: Total 29 lung cancer patients with hypercalcemia were studied, and most of them had squamous cell carcinoma in their histologic finding. The incidence of hypercalcemia was significantly higher between 50 and 69 years of age, and in the advancement of cancer stage. Although serum calcium level showed positive correlation with mass size, performance status, and bone metastasis, it was not significant statistically. Altered consciousness was significantly more frequent in the patients with higher serum calcium level. There were no differences in effectiveness among therapeutic regimens. Hypercalcemia was more frequently in the later stage of disease than during the initial diagnosis of lung cancer. Most of the patients died within 1 month after development of hypercalcemia. Conclusion: We concluded that hypercalcemia in lung cancer is related to extremely poor prognosis, and may be one of the causes of death and should be treated aggressively to prevent sudden deterioration or death.

• Progress in Medical Physics
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• 제17권2호
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• pp.83-88
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• 2006

Purpose: We Investigated to achieve high resolution magnetic resonance (MR) Imaging and spectra of human skin in vitro with using a 14.1 T MRI/MRS system, and to evaluate the hydration effect of a moisturizer by measuring the skin's water concentration. Materials and Methods: We used the Brukrer 14.1 T MRI/MRS system with a vertical standard bore that was equipped with a DMX spectrometer gradient system (200 G/cm at a maximum 40 A), RF resonators (2, 5 and 10 mm) and Para Vision software. Spin echo and fast spin echo pulse sequences were employed for obtaining the high resolution MR images. The 3D-localized point resolved spectroscopy (PRESS) method was used to acquire the MR spectra. Results: The high resolution MR images and spectra of human skin in vitro were successfully obtained on a 14.IT system. The water concentration of human skin after applying a moisturizer was higher than that before applying a moisturizer. Conclusions: The present study demonstrated that the high-resolution MR images and spectra of human skin from a high field MRS instrument could be applicable to evaluating the hydration state of the stratum corneum.

• Management & Information Systems Review
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• 제36권3호
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• pp.167-184
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• 2017

In this study, we investigated the effect of offline banking trust on smart banking trust. As influencing factors of smart banking trust, this study compared offline banking trust, smart banking's system quality, and information quality. For the empirical study, 186 questionnaire data were collected from smart banking users and the data were analyzed using Smart-PLS 2.0. As results, it was verified that there is trust transfer in FinTech service, by the significant effect of offline banking trust on smart banking trust. And it was proved that the effect of offline banking trust on smart banking trust is lower than that of smart banking itself. The contribution of this study can be seen in both academic and industrial aspects. First, it is the contribution of the academic aspect. Previous studies on banking were focused on either offline banking or smart banking. But this study, focus on the relationship between offline banking and online banking, proved that offline banking trust affects smart banking trust. Next, it is the industrial contribution. This study showed that offline banking characteristics of traditional commercial banks affect the trust of emerging smart banking service. This means that the emerging FinTech companies are not advantageous in the competition of trust building compared to traditional commercial banks. Unlike traditional commercial banks, the emerging FinTech is innovating the convenience of customers by arming them with new technologies such as mobile Internet, social network, cloud technology, and big data. However, these FinTech strengths alone can not guarantee sufficient trust needed for financial transactions, because banking customers do not change a habit or an inertia that they already have during using traditional banks. Therefore, emerging FinTech companies should strive to create destructive value that reflects the connection with various Internet services and the strength of online interaction such as social services, which have an advantage over customer contacts. And emerging FinTech companies should strive to build service trust, focused on young people with low resistance to new services.

• Sleep Medicine and Psychophysiology
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• 제9권2호
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• pp.106-114
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• 2002

Objectives: Daytime sleepiness is a common symptom and is associated with sleep behavior, sleep deprivation, and night shift, etc. It is also one of the most important symptoms of sleep disorders like obstructive sleep apnea (OSA). According to our survey on Korean literature, a few studies have dealt with daytime sleepiness, and we have not been able to locate any study comparing normal subjects with polysomnography-proven sleep disorder patients regarding daytime sleepiness. We aimed at comparing daytime sleepiness among normal healthy daytime workers, medical students being expected to have daytime sleepiness due to chronic sleep deprivation, and patients having sleep disorders diagnosed with polysomnography. We also studied the association between subjective daytime sleepiness and objective polysomnographic findings in patients with sleep disorders. Methods: One hundred three hospital workers, 137 medical students, and patients with sleep disorders were studied. Sleep disorders included OSA, periodic limb movements in sleep (PLMS), insomnia, and narcolepsy. The degree of subjective sleepiness in each group was measured by the Korean version of Epworth sleepiness scale and compared. The relationship between polysomnographic findings reflecting severity of sleep disorder in each patient group and subjective sleepiness was analyzed. As for patients with narcolepsy, the relationship between the mean sleep latency and subjective sleepiness was studied. Results: There was a significant difference of ESS score (F=68.190, dF=5.752, p<0.001) among daytime workers, medical students, and sleep disorder patients. In OSA patient group, the degree of the sleepiness had no significant correlation either with mean O2 satuaration (p=0.062) or with RDI (p=0.807). In PLMS patient group, there was no correlation between periodic limb movement index (PLMI) and subjective sleepiness (p=0.761). In narcolepsy patient group, the subjective sleepiness had no correlation with mean sleep latency measured with MSLT (p=0.055). Conclusion: We found a significant difference of subjective sleepiness among daytime workers, medical students, and patients with sleep disorders. However, no consistent correlation was found between severity of subjective sleepiness and objective polysomnographic findings reflecting severity of each sleep disorder. This research confirms that the evaluation of subjective sleepiness is important clinically, but it cannot substitute the objective measures such as nocturnal polysomnography and MSLT.

• Journal of Animal Science and Technology
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• 제46권5호
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• pp.743-752
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• 2004

This experiment was conducted to investigate the effect of filtered water with silicate minerals on growth performance and pork quality. A total of 64 pigs were allotted at 37.95 kg body weight to 4 treatments in a randomized complete block(RCB) design. Each treatment had 4 replicates and treatments were I) Con(basaI diet). 2) Si-I(consumed silicate mineral filtered water and silicate mineral additive during 4 weeks before market weight), 3) Si-Il( consumed silicate mineral filtered water and silicate mineral additive during 8 weeks before market weight) and 4) Si-I1I(consumed silicate mineral filtered water and silicate mineral additive during 12 weeks before market weight). Silicate complex was added at 0.8% to basal diet. All pigs were allowed to feed and water ad libitum for 12 weeks. During last 4 weeks, average daily gain(ADG) in Si-Il and Si-III group was higher than that in Si-I group(P < 0.05). However overall experimental period, there were no significant differences in growth performance among all treatments. Acceptability of cooked pork at 7th day after slaughter in Si-lII group was higher than that in Con group(P < 0.05). The value of pH of fresh pork at 6 hours after slaughter was lower in Si-I1I group than that in Con group(P < 0.05). TBA values of fresh pork at 14th day after slaughter were lower in Si-II and Si-III groups than Con group(P < 0.05). These results demonstrated that pork quality could be improved when pigs consumed both silicate mineral and silicate mineral filtered water. However, growth performance was not improved by the consumption of silicate mineral filtered water and silicate mineral.