• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.77-83
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• 2011

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures [10%, (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into $LN_2$. One-step dilution in straw was done in $25^{\circ}C$ water for 1 min, by placing vertically in the state of plugged-end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

• The Korean Journal of Financial Management
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• v.27 no.2
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• pp.1-33
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• 2010

This study explores if the higher initial returns and the poorer long-run performance observed in the IPOs markets are associated with the firms offered in the 'hot markets,' and then empirically examines the effect of optimistic investors' sentiment on this phenomenon, particularly in the aspects of both pricing mechanism and the opportunistic behavior of offering firms. We analyzed a total of 432 IPO firms for the years between 2001 and 2005. This analysis finds that the initial returns and long-run under-performances of 'IPOs in the hot market' are significantly higher than those of 'IPOs in the cold market.' This study also finds that the proxy variables for the optimistic investors' sentiment have a positive effect on the initial return and negative effect on the long-run performance. Finally, this research finds no difference of ownership structure, venture capital backed, and financial properties between hot market IPOs and cold market IPOs. R&D expenditure rate and financial qualities of IPOs are higher in the hot market than in the cold market. These results do not support the 'windows of opportunity' hypothesis that low quality firms take advantage of hot market condition for successful IPOs.

• The Korean Journal of Zoology
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• v.33 no.4
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• pp.461-467
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• 1990

To investigate the neurological effect of acrylamide, whole brain of Intoxicated mouse induced early hindlimbs ataxia was examined by using the methods of SDS-PAGE and two-dimensional electrophoresis. In the gel patterns by SDS-PAGE, when the patterns of each group were compared relatively, there were no remakable changes but in the patterns of 2D-PAGE, some protein alterations were observed. Especially, the spots containing 20 (14,500, 5.64) and 21 (19,900, 6.78) were disappeared, and the spots 9 (31,300, 5.82), 11 (31,300, 5.36) and 19 (16,400, 5.42) showed marked decrease relatively in the case of treatment group. Among these changed spots, the spot 20 (14,500, 5.64) showed higher quantity than that of control group but several spots containing the spots 11 (31,300, 5.36), and 19(16.400, 5.42) were identical or equal to those of control In quantity in the case of recovery group. It seems that acrylamide might already inhibit the brain protein synthesis mechanism at the time of onset of distal neuropathy by participation in the protein metabolism so as to impair the brain regulation ability followed by a malfunction of mouse central nervous system (CNS) and recovery is gradually progressed with the dose and duration dependence after the cessation of acrylamide administration.

• Ocean and Polar Research
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• v.30 no.4
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• pp.453-466
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• 2008

In order to examine the generation mechanism of long ocean waves along the west coast of Korea and to understand the amplification process of the long ocean waves, sea level, atmospheric pressure and wind data observed every minute from 2007 March 29 to 2007 April 1 were analyzed and onedimensional numerical ocean model experiments were performed. An atmospheric pressure jump propagated southeastward from Backryungdo to Yeonggwang along the west coast of Korea with speed of $13{\sim}27\;m/s$ between 2007 March 30 23:00 and 2007 April 1 1:30. Average magnitude of pressure jump was 4.2 hPa. As a moving atmospheric jump propagated from north to south along the coast, long ocean waves were generated and the sea level abnormally rose or fell at Anheung, Kunsan, Wido and Yeonggwang. Average amplitude of sea level rise (or fall) was about 113.6 cm. In a one-dimensional numerical ocean model, nonlinear shallow water equations were numerically integrated and a moving atmospheric pressure jump with traveling speed of 24 m/s was used as an external force. While the atmospheric pressure jump travels over 60 m depth ocean, a long ocean wave is generated. Because the propagation speed of the atmospheric jump is almost equal to that of the long ocean wave, Proudman resonance occurs and the long ocean wave amplifies. As the atmospheric pressure jump moves into the coastal area shallower than 60 m, the speed of the long ocean wave decreases and Proudman resonance effect decreases. However, the amplitude of the long ocean wave increases and wave length becomes shorter because of shoaling effect. When the long ocean wave hits the land boundary, amplitude of the long ocean wave drastically amplifies due to reflection. Data analysis and numerical experiments suggest that the southeastward propagation of an atmospheric pressure jump over the shallow ocean, which is a necessary condition for Proudaman resonance, generated the long ocean waves along the west coast of Korea on 2007 March 31 and the ocean waves amplified due to shoaling effect in the coastal area and reflection at the shore.

• BMB Reports
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• v.38 no.1
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• pp.77-81
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• 2005

The monocyte chemotactic protein-3 (MCP3), on chromosome 17q11.2-q12, is a secreted chemokine, which attracts macrophages during inflammation and metastasis. In an effort to discover additional polymorphism(s) in genes whose variant(s) have been implicated in asthma, we scrutinized the genetic polymorphisms in MCP3 to evaluate it as a potential candidate gene for asthma host genetic study. By direct DNA sequencing in twenty-four individuals, we identified four sequence variants within the 3 kb full genome including 1,000bp promoter region of MCP3; one in promoter region (-420T>C), three in intron (+136C>G, +563C>T, +984G>A) respectively. The frequencies of those four SNPs were 0.020 (-420T>C), 0.038 (+136C>G), 0.080 (+563C>T), 0.035 (+984G>A), respectively, in Korean population (n = 598). Haplotypes, their frequencies and linkage disequilibrium coefficients (|D'|) between SNP pairs were estimated. The associations with the risk of asthma, skin-test reactivity and total serum IgE levels were analyzed. Using statistical analyses for association of MCP3 polymorphisms with asthma development and asthma-related phenotypes, no significant signals were detected. In conclusion, we identified four genetic polymorphisms in the important MCP3 gene, but no significant associations of MCP3 variants with asthma phenotypes were detected. MCP3 variation/haplotype information identified in this study will provide valuable information for future association studies of other allergic diseases.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.237-241
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• 2010

Many policies have been implemented to mitigate the greenhouse gases in atmosphere overall of sectors. With considering the distinct characteristics of the food security, agricultural sector is no exception to this situation. To this regard, total amount of carbon which is emitted through all of the agricultural production process is calculated, and being based on this result, the demand for the introduction of agricultural production system with low carbon has been rising. Case studies on the application of life cycle assessment (LCA) technique to agricultural sector are found in many countries. For example, life cycle inventory (LCI) data bases of crop, farm infrastructure, fertilizer, farm machinery, and etc., have been constructed and provided by Ecoinvent (Swiss centre for life cycle inventories) of Swiss. In Japan, Top-down typed LCA methodology for agriculture is developed based on the inter-industry analysis, and is evaluated according to the productive method of crop. On the other hand, environmental impact assessment of agricultural system using LCA in Korea is just in the beginning stages. So it is required to assess environmental impact on agricultural fertilizer and pesticide, and to develop their flow modeling, and methodology of LCA of agricultural sector. Environmental impact assessment on agricultural materials, machinery, and infrastructure will also be carried out.

• Economic and Environmental Geology
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• v.45 no.4
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• pp.407-429
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• 2012

There has been much debates on the geologic age of the sediments of the Domi Basin, since age results varied after research methods and fossil groups. This study carried out palynological analysis and seismic interpretation to establish a stratigraphy and environmental reconstruction mainly based on fossil dinoflagellates and Seismic data from the Domi-1 and Sora-1 wells. The dinocyst assemblages found enabled zonation of the well sediment sequence resulting in 4 ecozones. Index fossils among dinocysts and palynomorphic substances indicate geologic age of the well ranges from Eocene to Pleistocene, and paleoenvironment varies from freshwater to inner-neritic marine. The fossil association also suggests strong relationship to Japanese Tertiary basins in Kyushu area in terms of stratigraphy and basin developmental history.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.7
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• pp.1067-1075
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• 2007

Twenty eight 3-4 month old castrated Black Bengal kids (Capra hircus) were used to determine the effects of source and level of dietary copper (Cu) concentration on their performance and nutrient utilization. Cu was supplemented (0, 10, 20 and 30 mg/kg diet DM) as copper sulfate ($CuSO_4$, $5H_2O$) or copper proteinate (Cu-P). Kids were fed a basal diet containing maize (19.5%), soybean (17.0%), deoiled rice bran (56.5%), molasses (4.0%), di-calcium phosphate and salt (1.0% each) and mineral and vitamin mixture (0.5% each) supplements at 3.5% of body weight to meet NRC (1981) requirements for protein, energy, macro minerals and micro minerals, excluding Cu. The basal diet contained 5.7 mg Cu/kg, 122.5 mg Fe/kg, 110 mg Zn/kg, 0.26 mg Mo/kg and 0.32% S. $CuSO_4$ or Cu-P was added to the basal diet at the rate of 10, 20 and 30 mg/kg. Kids were housed in a well ventilated shed with facilities for individual feeding in aluminum plated metabolic cages. Blood samples were collected from the jugular vein on d 0, 30, 60 and 90 to determine hemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), total leukocyte count (TLC) and serum enzymes (alkaline phosphatase, alanine transferase and aspertate transferase). A metabolism trial of 6 days duration was conducted after 90 days of experimental feeding. Statistical analysis revealed that source and level of Cu supplementation improved live weight gain (p<0.04) and average daily gain (p<0.01). No significant contribution of source and level of Cu to alter serum serum enzymes was evident. Goats fed Cu-P tended to have higher Hb, PCV and TEC than with $CuSO_4$ supplementation. Cu-P increased digestibility of ether extract (EE, p<0.02) and crude fiber (p<0.05) and showed an increasing trend (p<0.09) for digested crude protein (CP) and crude fiber (CF). Supplemental dose of Cu linearly improved (p<0.02) digestibilities of dry matter (DM), organic matter (OM), EE and nitrogen free extract (NFE). Though the absorption of nitrogen (N) was not affected (p>0.10) by both source and dose of Cu, N retention was affected (p<0.04) and there was a significant $Source{\times}Dose$ interaction (p<0.05). Final body weight (BW) was not influenced (p>0.10) by the source of Cu but increasing dose of Cu increased (p<0.04) the BW of kids. TDN intake (g/kg $W^{0.75}$) was higher (p<0.05) with the increased dose of Cu and there was a significant $Source{\times}Dose$ interaction. It was concluded that supplementation of Cu from different sources and varying dose level in a concentrate based diet may improve performance, nutrient utilization and plane of nutrition in castrated Black Bengal kids. The effects on performance and nutrient utilization are more pronounced with Cu-P than $CuSO_4$ supplementation. Higher dose of Cu showed better result than lower dose.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.10
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• pp.1395-1403
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• 2008

The objective of the present study was to estimate genetic parameters for economic traits in Markhoz goats. Data collected from 1993 to 2006 by the Markhoz goat Performance Testing Station in Sanandaj, Iran, were analyzed. The traits recorded as body weight performance at birth (BW), weaning (WW), six month (6MW), nine month (9MW), yearling (YW) and yearling fleece weight (YFW) were investigated. Least square analyses were used for estimation of environmental effects. Genetic parameters were estimated with single and multi trait analysis using restricted maximum likelihood (REML) procedures, under animal models. By ignoring or including maternal additive genetic effects and maternal permanent environmental effects, five different models were fitted for each trait. The effects of sex, type of birth, age of dam and year of birth on the all body weights were significant (p<0.01), but had no effects on YFW except year of birth. Age of kids had significant influences on WW and 6MW (p<0.01). A log likelihood ratio test was carried out for choosing the most suitable model for each trait. Total heritability estimates for YFW and growth traits varied from 0.16 for YFW and WW to 0.41 for YW. For all traits, maternal heritability was lower than direct heritability, ranging from 0.06 for BW to 0.01 for 6MW and 9MW. The magnitude of $c^2$ was more substantial for BW than the others, and relative importance was reduced from 0.12 for BW to 0.04 for 9MW. The direct additive genetic correlations estimates were positive and varied from 0.21 between BW-YW to 0.96 between WW-6MW. Direct additive genetic correlations between YFW and body weight traits were positive and ranged from 0.14 between BW-YFW to 0.67 between 6MW-YFW. For all traits, the corresponding estimates for phenotypic correlation were positive and lower than genetic correlations. The maternal additive genetic correlations between various traits were varied and ranged from -0.19 between 9MW-YFW to 0.96 between 6MW-9MW. The estimates of the maternal permanent environmental correlations between various traits were positive and ranged from 0.33 between WW-YFW to 0.93 between WW-6MW. Also, the environmental correlations between various traits ranged from 0.01 between BW-YFW and WW-YFW to 0.70 between 9MW-YW. Estimates of genetic parameters for various traits in this study confirm that selection should be applied on WW for genetic improvement in Markhoz goats.

• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.98-103
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• 2012

For screening of useful enzymes producing microorganisms from Meju, we isolated high lipase producing strains and their lipolytic enzyme activities were then tested. The lipolytic enzyme activities of isolated microorganisms were therefore tested on the Y124 strain. The gene sequence analysis of ITS from Y124 strain revealed Yarrowia lipolytica. Lipase production by the Y124 strain was studied in media containing various carbon sources. The Y124 strain drastically increased lipolytic enzyme activity in YPO media containing olive oil, as well as in YPDO media containing both olive oil and glucose. Maximal lipase production was achieved in YPD (yeast extract-peptone-D-glucose) media containing 0.7% olive oil when cultured at $30^{\circ}C$ for 8 hrs. The lipase produced from the Y124 strain showed the highest activity in p-NPO (p-nitrophenyl octanoate ($C_8$)), amongst the various p-nitrophenyl esters.