• Title/Summary/Keyword: 28S

Search Result 16,096, Processing Time 0.054 seconds

Effect of Defoliation Interval on Regrowth and Morphological Characters in White Clover (예취주기가 White Clover의 재생 및 형태적 특성에 미치는 영향)

  • 강진호
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.39 no.3
    • /
    • pp.245-255
    • /
    • 1994
  • Weak persistence of white clover (Trifolium repens L.) under continuous grazing management has been limited its availability in the mixture with grasses. The experiment was carried out to determine the effect of defoliation interval on the regrowth and morphological characters of the clover cultivars. Individual plants of Regal (large leaf), Grasslands Huia (medium-small leaf) and Aberystwyth S184 (small leaf) were grown in 15cm plastic pot containing a 1: 2: 1 soil:sand:Promix mixture for 55 days, and then clipped to remove all fully expanded leaves every 7, 14 or 28 days. For the analysis of the cultivar response, plants were sampled on the final harvest date(0), and 1, 3, 7, 14 and 28 days after the final harvest date. Harvested dry weight of all cultivars declined as defoliation interval was increased, and that of Regal was the highest compared to the other cultivars. During the regrowing period, increase of total plant dry weight was due to that of leaf and petiole dry weight, and that of Osceola was greater than the others. Although total leaf area and mean single leaf area were increased during the regrowing period, they were reduced with increased defoliation interval and those of Osceola were the greatest until 14-days regrowth. S184 possessed the most number of leaves and Osceola did the longest petiole since 14-days regrowth although the more defoliation, the less nunber of leaves per plant and the shorter petiole. Stolon length and growing tips of all cultivars increased steeply during regrowth, while they were decreased with increased defoliation interval and those of S184 were the highest. More frequent defoliation had detrimental effects on regrowth of white clover, although larger leaf type was productive but less persistent in a mixture with grasses than smaller leaf type.

  • PDF

Suppression of a Residue 173 Mutant Form on Aggregation of Tryptophan Synthase α-Subunits from Escherichia coli (대장균 트립토판 중합효소 α 소단위체의 응집 형성에 미치는 잔기 173 치환체의 억제 효과)

  • Jeong, Jae Kap;Park, Hoo Hwi;Lim, Woon Ki
    • Journal of Life Science
    • /
    • v.32 no.9
    • /
    • pp.729-733
    • /
    • 2022
  • Aggregation of normally soluble proteins can cause disease-related problems. Tryptophan synthase α-subunit (αTS) in E. coli adopts one of most popular structural scaffolds, the TIM barrel fold. Previous mutagenesis of the αTS gene resulted in many aggregation-prone mutant proteins. Here, Y173F (Tyr at residue 173 to Phe) substitution, which imparts increased stability, was tested for its ability to suppress aggregation of aggregation-prone mutant proteins (Y4C, S33L, P28L, P28S, G44S, D46N, P96L, and P96S). Aggregation was suppressed in all eight severe aggregate-forming mutants (all differing in their mutation positions), by the Y173F replacement. P28L αTS, which was available in pure form, was further analyzed and showed reduced secondary structure content, lower stability, and a looser structure with more exposed hydrophobic surface compared to the wild type protein. A double mutant P28L/Y173F protein showed almost no indication of these changes compared to the wild type protein. We hypothesized that Tyr at position 173 in αTS is positioned at the hydrophobic core and may serve to suppress the aggregation of this protein caused by other residues. Important residue (s) could be working widely in the prevention/suppression of protein aggregation.

Functional Analysis of Gene ID1103135 Encoding a 3-Phytase Precursor Homologue of Streptomyces coelicolor (Streptomyces coelicolor의 3-Phytase 상동성 유전자 ID1103135의 기능분석)

  • 김미순;강대경;이홍섭;연승우;김태영;홍순광
    • Korean Journal of Microbiology
    • /
    • v.40 no.2
    • /
    • pp.81-86
    • /
    • 2004
  • Among the annotated ORFs of Streptomyces coelicolor, SCO7697 was supposed to encode for phytase (myo-inositol hexakisphosphate phosphohydrolase). The DNA fragment containing SCO7697 was cloned by the PCR from the chromosomal DNA of S.coelicolor A3(2)M. The cloned fragment was introduced into E. coli expres-sion vector, pET28a(+), to yield two recombinant plasmids, pET28-SP and pET28-LP, which were designed to encode different length of proteins. When the pET28-SP and pET28-LP were introduced into E. coli BL21, the transformants successfully overexpressed recombinant proteins, but the molecular weights of the expressed pro-teins were appeared bigger than those of expected in SDS-polyacrylamide gel electrophoresis. The shift of cul-tural temperature from 37 to $30^{\circ}C$ made most of expressed protein be solubilized. The expressed protein, however, did not show any phytase activity. When the DNA fragment with its own promoter placed on the E. coli-Streptomyces vector, pWHM3, and introduced into S. lividans, the phytase activity was not detected either. These results suggest that even though the SCO7697 was annotated as a probable phytase with high probability (E value is $6e^{-89}$), the real product doest not have phytase activity.

Molecular Phylogeny of Veneroidea (Bivalvia: Heteroconchia) on the Basis of Partial Sequences of 28S rRNA Gene (일부 28S rRNA 염기서열을 이용한 백합 상과 패류의 계통분류)

  • Kim, Sei-Chang;Kim, Jae-Jin;Hong, Hyun-Chul
    • The Korean Journal of Malacology
    • /
    • v.21 no.2 s.34
    • /
    • pp.147-161
    • /
    • 2005
  • To elucidate the phylogenetic relation of the superfamily Veneroidea, we obtained partial 28S rRNA sequences of 14 heterodonts and three pteriomorphs which were collected from Korea and the sequence data of related taxa from GenBank, and analyzed maximum parsimony with PAUP program 750 of the nucleotide positions were variable, 560 of which were informative under conditions of parsimony. Total tree length was 2,765, and consistency index, homoplasy index (HI), and Retention index was 0.4843, 0.5157, and 0.6291, respectively. Intraspecific variation of 28 rRNA of Corbicula fluminea and Sinonovacula constricta was 3.1% and 1.3%, respectively. Pitarinae-Cyclininae-Meretrinae group had a clade and Samaranginae, Chioninae, and Dorsininae were clustered.

  • PDF

Electrical Characteristics of 28w class-Piezoelectric Transformer for Fluorescent Lamp Ballast as a function of Load Resistance (형광등 안정기용 고출력 (28W급)압전트랜스포머의 부하저항에 따른 전기적 특성)

  • Hwang, S.M.;Lee, J.S.;Yoo, J.H.;Park, C.Y.;Lee, S.H.;Lee, S.I.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2001.05c
    • /
    • pp.135-139
    • /
    • 2001
  • Contour vibration mode piezoelectric transformers were designed and fabricated to the square plate with size of $27.5{\times}27.5{\times}2.5(2.6,\;3.0)mm^3$ using PNW-PMN-PZT ceramics. Electrical characteristics of the piezoelectric transformer were investigated for fluorescent lamp ballast application. The electrical properties and characteristic temperature rise were measured using oscilloscope and infrared temperature sensor. A 28W fluorescent lamp was successfully driven by the fabricated transformers. After driving the lamp using Power Amplifier for 24 min, the output power, efficiency and characteristic temperature rise of PT2 piezoelectric transformer showed the appropriate values of 28.01 W, 99.43% and $11^{\circ}C$, respectively. The electronic ballast using PT2 piezoelectric transformer showed an excellent output power of 28.85 W and efficiency of 86.3%, respetively.

  • PDF

Study on Environmental Hazards of Alternatives for PFOS (PFOS 대체물질의 환경유해성에 관한 연구)

  • Choi, Bong-In;Chung, Seon-Yong;Na, Suk-Hyun;Shin, Dong-Soo;Ryu, Byung-Taek
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.38 no.6
    • /
    • pp.317-322
    • /
    • 2016
  • While PFOS sodium salt ($C_8F_{17}SO_3Na$) was not degraded by microorganisms for 28 days, the 4 alternatives were biodegraded at the rates of 21.6% for $C_{25}F_{17}H_{32}S_3O_{13}Na_3$, 20.5% for $C_{15}F_9H_{21}S_2O_8Na_2$, 15.8% for $C_{23}F_{18}H_{28}S_2O_8Na_2$ and 6.4% for $C_{17}F_9H_{25}S_2O_8Na_2$, respectively. The acute toxicity test using Daphnia magna was conducted for 48 hours, the half effective concentration ($EC_{50}$) of PFOS sodium salt ($C_8F_{17}SO_3Na$) was evaluated in 54.5 mg/L. While the 4 alternatives did not show any effect at 500.0 mg/L. The surface tension of the PFOS salt ($C_8F_{17}SO_3Na$) is 46.2 mN/m at a concentration of 500.0 mg/L. While the surface tension of the 4 alternatives was found to be superior to PFOS sodium salt ($C_8F_{17}SO_3Na$). The surface tension of $C_{23}F_{18}H_{28}S_2O_8Na_2$ (20.9 mN/m) has the lowest, followed by $C_{15}F_9H_{21}S_2O_8Na_2$ (23.4 mN/m), $C_{17}F_9H_{25}S_2O_8Na_2$ (27.3 mN/m), $C_{25}F_{17}H_{32}S_3O_{13}Na_3$ (28.2 mN/m). The four kinds of alternatives ($C_{15}F_9H_{21}S_2O_8Na_2$, $C_{17}F_9H_{25}S_2O_8Na_2$, $C_{23}F_{18}H_{28}S_2O_8Na_2$, $C_{25}F_{17}H_{32}S_3O_{13}Na_3$) were found to be superior to PFOS sodium salt ($C_8F_{17}SO_3Na$) in terms of biodegradation, Daphnia sp. acute toxicity and surface tension, and thus they were considered applicable as PFOS alternatives. Especially biodegradation rate of $C_{15}F_9H_{21}S_2O_8Na_2$, $C_{23}F_{18}H_{28}S_2O_8Na_2$ and $C_{25}F_{17}H_{32}S_3O_{13}Na_3$ was relatively high as 15.8~21.6%, and Daphnia sp. acute toxicity and surface tension were considerably superior (surface tension 39~55%) to PFOS sodium salt. Therefore, these alternatives are considered to be available as an alternative of PFOS.