• Title/Summary/Keyword: 28S

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[ $2{\beta}$ ], $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic Acid Induces the Apoptosis of Human Hepatoma HepG2 Cells ($2{\beta}$, $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid처리에 의한 인간 간암세포주 HepG2의 apoptosis 유도)

  • Yoo, Ki-Hyun;Lee, Jong-Min;HwangBo, Jeon;Song, Myoung-Chong;Yang, Hye-Joung;Baek, Nam-In;Kim, Soung-Hoon;Kim, Dae-Keun;Kwon, Byoung-Mok;Park, Mi-Hyun;Chung, In-Sik
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.270-275
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    • 2006
  • [ $2{\beta},\;3{\alpha}$ ], 23-trihydroxyrus-12-ene-28-oic acid was isolated from Trapa pseudoincisa S. et Z. It has a common structure of pentacyclic triterpenes and belongs to the amyrin ursolic acid group. The cytotoxic effect of this compound was investigated in human hepatoma cell line HepG2. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid showed dose-dependent cytotoxicity in HepG2 cells. Confocal microscopy data showed that green fluorescence was increased in $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid treated-HepG2 cells in a time-dependent manner. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid also increased the sub-G1 cell population of HepG2 cells as well as ladder-like DNA fragmentation. Taken together, our results indicate that $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid induced apoptosis in HepG2 cells.

Characteristics and Antimicrobial Effects of Novel Burkholderia cepacia No. 15-2 Isolated from Compost (퇴비로부터 분리된 Burkholderia cepacia No.15-2의 특성과 항균 효과)

  • Yun, Soon-Il
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.421-428
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    • 2003
  • To develop the functional-compost containing antifungal substance by using antagonistic microorganisms, Spinacia oleracea L and Rhizoctonia solani Kuhn O-28 were used as a model plant and phytopathogen, respectively. Total 80 strains were isolated from the compost of various waste foods mixture processed for a year. Among them, No.15-2 strain was selected due to its highest antifungal activity against R. solani Kuhn O-28 and was identified phyno- and phylogenotypically as Burkholderia cepacia genomovar V. which is rare probability in pathogen, by 16S rDNA sequencing and specific primer pair PCR method. B. cepacia No.15-2 preferentially dominated during the compost and its cell numbers were maintained almost $${\times}$10^{13}$ cuf/g for 15 days. The morbidity caused by R. solani Kuhn O-28 in S. oleracea L cultivation was reduced to 40% by addition of B. cepacia No.15-2. In conclusion, the antifungal compost using B. cepacia No.15-2 could be applied to biocontrol of various crops blights caused by fungal pathogen.

Strength and Non-fracture Test of Ready Mixed Concrete Using Polypropylene Fiber (폴리프로필렌 섬유를 혼입한 레미콘의 강도 및 비파괴 시험)

  • Nam, Ki-Sung;Youn, Dong-Chae;Yoon, Yeo-Hoon;Sung, Chan-Yong
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2003.10a
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    • pp.403-406
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    • 2003
  • This study is performed to evaluate the strength and nan-fracture test of the ready mixed concrete(RMC) using polypropylene. The slump is reached in $8{\pm}2cm$ of each RMC using polypropylene or without polypropylene, air content is reached in $4.5{\pm}1.5%$, the chloride content is below $0.3kg/m^3$. The compressive strength of RMC not using polypropylene is appeared over $210kgf/cm^2$ at the curing age 7 days and $239kgf/cm^2$ at the curing age 28 days. The compressive strength of RMC using polypropylene is appeared over $188kgf/cm^2$ at the curing age 7 days and $238kgf/cm^2$ at the curing age 28 days. The dynamic modulus of elasticity of RMC not using polypropylene is appeared over $298{\times}10^3kgf/cm^2$ at the curing age 7 days and $342{\times}10^3kgf/cm^2$ at the curing age 28 days. The RMC using polypropylene is appeared over $284{\times}10^3kgf/cm^2$ at the curing age 7 days and $238{\times}10^3kgf/cm^2$ at the curing age 28 days. The pulse velocity of RMC not using polypropylene is appeared over 4,198m/s at the curing age 7 days and 4,382m/s at the curing age 28 days. The RMC using polypropylene is appeared over 4,182m/s at the curing age 7 days and 4,342m/s at the curing age 28 days.

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