• 한국약용작물학회지
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• 제17권1호
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• pp.26-32
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• 2009

The region containing 18S rRNA gene, ITS 1 and part of the 5.8S rRNA gene of the Atractylodes japonica Koidz was amplified by PCR and the product cloned in a pBluescript SK II plasmid. DNA sequence of the cloned DNA was determined and submitted to the GenBank (accession number EU678363). Phylogenetic analysis of the ITS 1 DNA showed close similarity with the other plant species of the family Compositae. The extract of the plant materials of five different members of the family Compositae was analyzed by HPLC to detect atractylon. Extract of the A. japonica Koidz showed presence of significant amount of atractylon. However, noticeable amount of atractylon was not detected by the same analyses from the extracts of the other plants belonging to the family Compositae including Artemisia capillaris, Chrysantemum zawadskii, Eclipta prostrata or Taraxacum platycarpum.

• 미생물학회지
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• 제26권4호
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• pp.312-317
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• 1988

The genes for ribosomal RNA in Rhizobium meliloti and Bradyrhizobium japonicum were analyzed by southern hybridization of BamHI, EcoRI, HindIII digested chromosomal DNA with purified 5' $^{32}P$-labeled 16S and 23S rRNA. The big differences in the hybridization pattern of both rhizobia were found. The comparative results were discussed in relation to the copy number and conservativity of restriction sites in the rRNA genes of both rhizobia.

• 한국미생물·생명공학회지
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• 제40권3호
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• pp.268-273
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• 2012

인도네시아 지열지대의 미생물 다양성을 16S rRNA 염기서열 분석을 통해 조사하였다. 전체적으로 어떠한 phylum 계통군에도 포함되지 않는 unclassified bacteria가 20.0-26.5% 존재하였으며 sampling 지역에 상관없이 Proteobacteria가 우점 phylum 계통군으로 나타났다. Cisolok 주변의 지열 지역을 조사한 결과 ${\beta}$-Proteobacteria (27.1%)와 Cyanobacteria (11.0%)가 높은 비율을 차지한 반면 Kamojang의 화산 주변 지역의 경우에는 ${\gamma}$-Proteobacteria (51.5%) 그리고 Aquificales (12.9%)가 우점 계통군으로 나타났다. 또한 Likupang 열수구의 경우에는 ${\gamma}$-Proteobacteria (33.3%)와 Bacteroidetes (27.6%)가 높은 비율로 나타났다. 본 연구를 통해 인도네시아 각 지열지대에 분포하는 미생물 군집은 각 지역의 환경적인 특징 (극한 지열 및 해양 서식지)과 밀접한 연관성이 있음을 알 수 있었다.

• 생명과학회지
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• 제12권2호
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• pp.158-163
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• 2002

남조류 Microcystis aeruginosa를 무균적으로 분리하기 위해 낙동강 물금지역의 수화를 멸균 증류수로 vortex 전처리를 하였으며, 세균제거 및 무균상태를 계속 유지하기 위하여 항생물질(ampicillin 150 $\mu$g/$m\ell$, neomycin 25 $\mu$g/$m\ell$)을 배지에 첨가하고, 독립 집락으로 형성시켜 오염 기회를 줄이기 위하여 0.7% agarose로 고형화시킨 CB고체배지에서 3$0^{\circ}C$, 40 $\mu$mol m$^{-2}$ s$^{-1}$ 광 조건으로 배양하였다. 그 결과 분리되어진 26개의 Microcystis aeruginosa colony 중 3개의 무균 균주만이 확보되었다. 3개의 무균균주를 16S rRNA primer를 이용하여 PCR 증폭한 결과 M. aeruginosa AF 139292와 99.5에서 100%의 상동성을 가지는 것으로 나타났다.

• Genomics & Informatics
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• 제13권2호
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• pp.26-30
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• 2015

nc886 (=vtRNA2-1, pre-miR-886, or CBL3) is a newly identified non-coding RNA (ncRNA) that represses the activity of protein kinase R (PKR). nc886 is transcribed by RNA polymerase III (Pol III) and is intriguingly the first case of a Pol III gene whose expression is silenced by CpG DNA hypermethylation in several types of cancer. PKR is a sensor protein that recognizes evading viruses and induces apoptosis to eliminate infected cells. Like viral infection, nc886 silencing activates PKR and induces apoptosis. Thus, the significance of the nc886:PKR pathway in cancer is to sense and eliminate pre-malignant cells, which is analogous to PKR's role in cellular innate immunity. Beyond this tumor sensing role, nc886 plays a putative tumor suppressor role as supported by experimental evidence. Collectively, nc886 provides a novel example how epigenetic silencing of a ncRNA contributes to tumorigenesis by controlling the activity of its protein ligand.

• 한국어병학회지
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• 제26권2호
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• pp.77-88
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• 2013

본 연구에서는 2012년 8월, 우리나라 울산 소재의 강도다리 양식장에서 뚜렷한 증상 없이 강도다리 폐사가 발생하여 그 원인을 밝히고자 하였다. 기생충, 세균, 바이러스 검사를 수행하였으며, 내부 장기에서 균이 순수 분리되어 해당 분리균주의 표현형 및 유전적 특성을 분석하였다. 순수 배양된 균체를 확인하여 계대 배양하여 생화학적 성상과 16S rRNA 유전자와 capsular polysaccharide (CPS) 유전자의 염기서열 분석 결과 Photobacterium damselae subsp. piscicida으로 동정되었다. Photobacterium damselae subsp. piscicida와 Photobacterium damselae subsp. damselae는 TCBS agar 배지의 균주 배양 유무, 16S rRNA 및 CPS 유전자 분석을 통해 구분할 수 있었다. 실험 균주는 ofloxacin과 gentamycin에 대해서 감수성을 나타냈으며, 배양 온도에 따른 발육시험 시 $18^{\circ}C$ 및 $25^{\circ}C$에서 시험한 다른 균주에 비해 유의적으로 높은 생장율을 나타내었다.

• ALGAE
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• 제38권1호
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• pp.1-22
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• 2023

An enormous body of research is focused on finding ways to commercialize carotenoids produced by the unicellular green alga, Haematococcus, often without the benefit of a sound phylogenetic assessment. Evidence of cryptic diversity in the genus means that comparing results of pigment studies may be confounded by the absence of a phylogenetic framework. Moreover, previous work has identified unnamed strains that are likely candidates for species status. We reconstructed the phylogeny of an expanded sampling of Haematococcus isolates utilizing data from nuclear ribosomal markers (18S rRNA gene, 26S rRNA gene, internal transcribed spacer [ITS]-1, 5.8S rRNA gene, and ITS-2) and the rbcL gene. In addition, we gathered morphological, ultrastructural and pigment data from key isolates of Haematococcus. Our expanded data and taxon sampling support the concept of a new species, H. privus, found exclusively in North America. Despite overlap in numerous morphological traits, results indicate that ratios of protoplast length to width and akinete diameter may be useful for discriminating Haematococcus lineages. High growth rate and robust astaxanthin yield indicate that H. rubicundus (SAG 34-1c) is worthy of additional scrutiny as a pigment source. With the description of H. privus, the evidence supports the existence of at least five, species-level lineages in the genus. Our phylogenetic assessment provides the tools to frame future pigment investigations of Haematococcus in an updated evolutionary context. In addition, our investigation highlighted open questions regarding polyploidy and sexuality in Haematococcus which demonstrate that much remains to be discovered about this green flagellate.

• Journal of Species Research
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• 제8권3호
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• pp.249-258
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• 2019

Owing to a distinct environmental regime and anthropogenic effects, freshwater bacterial communities of urban streams are considered to be different from those of large freshwater lakes and rivers. To obtain unrecorded, freshwater bacterial species in Korea, water and sediment samples were collected from various urban streams of the Han River watershed in 2018. After plating the freshwater samples on R2A agar, approximately 1000 bacterial strains were isolated from the samples as single colonies and identified using 16S rRNA gene sequence analyses. A total of 26 strains, with >98.7% 16S rRNA gene sequence similarity with validly published bacterial species but not reported in Korea, were determined to be unrecorded bacterial species in Korea. The unrecorded bacterial strains were phylogenetically diverse and belonged to four phyla, six classes, 12 orders, 16 families, and 21 genera. At the generic level, the unreported species were assigned to Nocardioides, Streptomyces, Microbacterium, Kitasatospora, Herbiconiux, Corynebacterium, and Microbacterium of the class Actinobacteria; Paenibacillus and Bacillus of the class Bacilli; Caulobacter, Methylobacterium, Novosphingobium, and Porphyrobacter of the class Alphaproteobacteria; Aquabacterium, Comamonas, Hydrogenophaga, Laribacter, Rivicola, Polynucleobacter, and Vogesella of the class Betaproteobacteria; Arcobacter of the class Epsilonproteobacteria; and Flavobacterium of the class Flavobacteriia. The details of the 26 unreported species, including Gram reaction, colony and cell morphology, biochemical properties, and phylogenetic position are also provided in the strain descriptions.

• Journal of Species Research
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• 제13권1호
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• pp.100-104
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• 2024

The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Daegu and Cheongju city, Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Among 13 strains, 11 strains were previously reported, but two strains were unreported from the Republic of Korea. The two unrecorded yeast strains, GW1-3 and PG1-1-10C, belong to the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes). The two strains had oval-shaped and polar budding cells. This research showed the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

• 대한의생명과학회지
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• 제26권3호
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• pp.149-156
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• 2020

We developed a simple and rapid method for detecting vancomycin resistance genes, such as vanA and vanB, using loop-mediated isothermal amplification (LAMP). To identify not only vancomycin resistance genes, but also the genus Enterococcus, primers were designed for vanA, vanB, and 16S rRNA. Screening for vancomycin susceptibility in Enterococcus was performed using Etest (bioMérieux Inc). The results of the LAMP assay were compared to those of real-time RT-PCR. The optimal conditions for the LAMP assay were 65℃ for 60 min. The detection limits of the LAMP assay for vanA, and vanB were 2 × 10² copies/reaction. Compared to RT-PCR, the sensitivities and specificities of LAMP for 16S rRNA, vanA, and vanB were 100/100%, 100/100%, and 100/100%, respectively. The vanA genotype-vanB phenotype accounted for 57.5% (46/80) of the vancomycin-resistant Enterococci samples collected from 2016 to 2019. In conclusion, the LAMP assay developed in this study showed high sensitivity and specificity for vancomycin-resistant genes. Moreover, due to the simplicity and rapidity of the LAMP assay, its use can be very useful in clinical microbiology laboratories.