• Title/Summary/Keyword: 20M10

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Regeneration and Maturation Induction of Free-Living Gametophytes of Undariopsis peterseniana for their Mass Production (넓미역(Undariopsis peterseniana)의 대량생산을 위한 유리배우체의 재생 및 성숙 유도)

  • Hwang, Eun-Kyoung;Shim, Cheol-Hong;Ha, Dong-Soo;Gong, Yong-Gun;Park, Chan-Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.2
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    • pp.155-161
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    • 2011
  • Induction of regeneration and maturation of the free-living gametophytes of Undariopsis peterseniana (Kjellman) Miyabe et Okamura was studied at four temperatures (5, 10, 15, and $20^{\circ}C$), four levels of irradiance (5, 10, 20, and 40 ${\mu}mol$ $m^{-2}\;s^{-1}$) and three photoperiods (14:10, 12:12, and 10:14 h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under conditions of $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}\;s^{-1}$, 10:14 h (L:D), whereas the conditions for male gametophytes were slightly different at $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}\;s^{-1}$, 14:10 h (L:D). The sexual maturation of female and male gametophytes took place under $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}\;s^{-1}$, 14:10 h (L:D) and $10^{\circ}C$, 10 ${\mu}mol\;m^{-2}\;s^{-1}$, 10:14 h (L:D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for artificial seed production of U. peterseniana.

Effects of Temperature, Photon Irradiance, and Photoperiod on the Growth of Embryos of Sargassum horneri in Laboratory Culture (괭생이모자반(Sargassum horneri) 유배의 생장에 미치는 온도, 광량, 광주기의 영향)

  • Kim, Nam-Gil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.1
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    • pp.76-81
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    • 2015
  • The effects of temperature, photon irradiance, and photoperiod on the growth of Sargassum horneri embryos were examined for female plants collected at Chokpo in Tongyeoung, Gyeongnam Prefecture, Korea on 27 December 2011. Mature plant receptacles were detached, and fertilized eggs were cultured in the laboratory at temperatures of $5-25^{\circ}C$ with photon irradiances of $10-80{\mu}mol\;m^{-2}s^{-1}$ under 14L:10D and 10L:14D photoperiods. Germination and embryo growth were rapid at $20-25^{\circ}C$ and $40{\mu}mol\;m^{-2}s^{-1}$ under 14L:10D. The number of rhizoids in the germinated young thalli was high at high photon irradiances ($40-80{\mu}mol\;m^{-2}s^{-1}$) and $15-20^{\circ}C$. At $20^{\circ}C$ and 14L:10D, vigorous growth of main and lateral branches was observed; however, at $5^{\circ}C$ under both photoperiods, plant growth decreased markedly. In the present study, S. horneri grew well at high temperatures ($20-25^{\circ}C$) and high photon irradiances ($40-80{\mu}mol\;m^{-2}s^{-1}$) with a 14L:10D photoperiod.

Low IF Resistive FET Mixer for the 4-Ch DBF Receiver with LNA (LNA를 포함하는 4채널 DBF 수신기용 Low IF Resistive FET 믹서)

  • 민경식;고지원;박진생
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
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    • 2002.11a
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    • pp.16-20
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    • 2002
  • This paper describes the resistive FET mixer with low IF for the 4-Ch DBF(Digital Beam Forming) receiver with LNA(Low Noise Amplifier). This DBF receiver based on the direct conversion method is generally suitable for high-speed wireless mobile communications. A radio frequency(RF), a local oscillator(LO) and an intermediate frequency(IF) considered in this research are 2.09 ㎓, 2.08 ㎓ and 10㎒, respectively. The RF input power, LO input power and Vgs are used -10㏈m, 6㏈m and -0.4 V, respectively. In the 4-Ch resistive FET mixer with LNA, the measured IF and harmonic components of 10㎒, 20㎒, 2.09㎓ and 4.17㎓ are about -12.5 ㏈m, -57㏈m, -40㏈m and -54㏈m, respectively. The IF output power observed at each channel of 10㎒ is about -12.5㏈m and it is higher 27.5 ㏈m than the maximum harmonic component of 2.09㎓. Each IF output spectrum of the 4-Ch is observed almost same value and it shows a good agreement with the prediction.

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Effect of Boric Acid on In Vitro Pollen Germination in Transgenic Plants Expressing Monoclonal Antibodies (단일항체를 발현하는 형질전환 식물체의 In Vitro 화분발아에 대한 Boric Acid의 영향)

  • Ahn, Mi-Hyun;Lee, Kyung-Jin;Ko, Ki-Sung
    • Journal of Plant Biotechnology
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    • v.34 no.4
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    • pp.339-346
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    • 2007
  • Pollen germination viability is an essential factor to produce seeds from pollination and fertilization, which are required to maintain plant generation. In this study, we tried to identify the effect of boric acid on pollen germination and tube grouch in non-transgenic and transgenic plants expressing monoclonal antibodies (anti-colorectal cancer mAb CO17-1A, anti-breast cancer mAb BR55, and anti-rabies virus mAb57). The pollen of non-transgenic plant was treated with different concentration of boric acid (0, 5, 10, 15, 20, $40{\mu}g/mL$) in germination buffer to investigate its effect on in vitro pollen germination. At $20{\mu}g/mL$ of boric acid, tile pollen germination rate was the highest (49.5%) compared to other concentrations. In general, the germination rate significantly increased 3-10 folds in boric acid ($20{\mu}g/mL$) treated group in non-transgenic and transgenic plants. Also, the pollen tube length increased in boric acid ($20{\mu}g/mL$) treated groups. In the treated group, the pollen tube length increased until 3 h boric acid treatment and decreased after the 3 h, indicating that the 3 h is the most appropriate incubation time period. Western blot analysis showed that the mAb transgene expression was more stable in leaf than pollen in transgenic plants. This study suggested that $20{\mu}g/mL$ of boric acid is ideal concentration to induce in vitro pollen germination of transgenic plants expressing therapeutic monoclonal antibodies, indicating stable pollination and fertilization in transgenic plants.

Distribution, flight altitude, and habitat use of birds at the Demonstration Offshore Wind Farm in the Southern Part of the West Sea (서남해 해상풍력 실증단지에서 조류의 분포, 비행고도 그리고 서식지 이용)

  • Jae-Gyun Lim;Jung-Soo Kim;Seung-Jong Jin
    • Journal of Wind Energy
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    • v.14 no.4
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    • pp.75-86
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    • 2023
  • We observed five species and 163 individuals of birds in the Demonstration Offshore Wind Farm in the southern part of the West Sea in Korea, and 33 species and 4,023 individuals from Gusipo to Dongho Beach. The most dominant bird in both areas was the black-tailed gull, Larus crassirostris. The flight height of birds for collision risk (wind turbine and bird) was divided into two groups. First, the safe height was from sea level to 20 m (< 20 m), with no effect from the action of revolving blades. Second, the danger height was more than 20 m from sea level (> 20 m), with a collision risk from the action of revolving blades. Birds flying a safe height (< 20 m) were 83.9% (271 individuals) and danger height (> 20 m) were 16.1% (52 individuals). Also, 11.7% of birds (35 individuals) used the inside of the Demonstration Offshore Wind Farm as a habitat for foraging and resting, and 88.3% birds used the outside. We suggest that the risk of collision with the wind farm was low due to the relatively low flight height of birds and distance between wind turbines (> 800 m).

Preferred Orientation and Microstructure of Zinc Electrodeposit in acid Chloride Solution (우선배향과 두절경조직)

  • 예길촌;박계생;손경옥
    • Journal of the Korean institute of surface engineering
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    • v.16 no.4
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    • pp.173-187
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    • 1983
  • Zinc was electrodeposited at temperature from 20$^{\circ}C$ to 60$^{\circ}C$ over the ranges of the current density from 2 to 20 A/dm2 in acid chloride bath. The cathode overpotentials increased with increasing current density and decreasing tem-perature. The (10$.$3)-(10$.$2) preferred orientation developed at cathode overpotentials below about 450mV, the (10$.$3)(10$.$2)-(10$.$1) texture developed at overpotentials between 500mV and 950mV, and the (00$.$1) (10$.$3) texture developed at cathode overpotentials about 1000mV. The (00$.$1) (10$.$3) preferred orientation was also formed at the lower potentials between 400mV and 850mV at temperatures above 40$^{\circ}C$. The preferred orientations of the zinc deposits was discussed was discussed with both cathode overpo-tential and surface energy of deposit lattice planes. The pyramid type of structure with macrostep developed at low cathode overpotentials and the truncated pyramidal type developed at higher overpotenial.

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Analysis of the Effect of Differences in Spatial Resolution of Land-use/cover Data on the Simulation of CALPUFF (토지피복 자료의 해상도 차이가 CALPUFF 농도 모의에 미치는 영향 분석)

  • Hwang, Suyeon;Ham, Jungsoo;Lee, Youngjin;Choi, Jinmu
    • Korean Journal of Remote Sensing
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    • v.37 no.5_3
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    • pp.1461-1473
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    • 2021
  • The purpose of this study is to ascertain how the level of resolution of land cover data affects on the local distribution and diffusion of fine dust. the CALPUFF model, which considers the spatio-temporal terrain conditions and changes in weather conditions, was used to estimate PM10 concentration in the Pyeongchon, Anyang-si, Gyeonggi-do. Three different resolutions of land cover data including 20 m, 50 m, and 100 m were compared as the input of the modeling. Using higher resolution land cover data (20 m), the wind speed of the simulated region was the largest and the PM10 concentration was the lowest. Through this study, we confirm that the resolution level of land-use/cover data can affect the local distribution and diffusion of fine dust, which can be detected by CALPUFF. Therefore, when using CALPUFF to simulate fine dust in the future, it can be suggested that checking the impact on spatial resolution according to the form of land cover in advance and proceeding with the simulation can achieve mote accurate results.

Estimation Of Vertical Structure Of Dispersion Coefficients Off Wolsong

  • Hagn, Sangbok
    • 한국해양학회지
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    • v.15 no.1
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    • pp.58-61
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    • 1980
  • Water movements at nine different layers were observed from July 31 to August 2, 1976 at station H, which has water depth 42 meters and 3km far from the coast of Wolsong, and dispersion coefficients were computed from these current data, then relative magnitudes of dispersion coefficients were calculated at each layers. Relative magnitudes of dispersion coefficients based on the surface value are 62% at 5m layer, 24% at 10m layer, 20% at 15m and 20m layers, 17% at 25m layer, 24% at 30m layer, 28% at 35m layer and 15% at bottom layer, respectively.

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Purification and Characteristic Properties of DNA Polymerase $\alpha$ from Sea-Urchin, Hemicentrotus pulcherrismus (말똥 성게의 DNA Polymerase $\alpha$의 정제와 특성)

  • HA Mi-Suck;RYU Beung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.2
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    • pp.136-145
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    • 1987
  • From the sea-urchin, Hemicentrotus pulcherrismus, we have purified by four column chromatographic steps for DNA polymerase $\alpha$ activity. The molecular weight of DNA polymerase u was determined to be around 137,000-138,000 by Sephadex G-200 gel filtration and SDS-polyacrylamide gel electrophoresis. The purified enzyme had the optimal activity at pH 7.4. This enzyme showed to be a function of the metal ion $K^+,\;Na^+$\;and\;Mg^{2+}$ employed as activators, the optimum $K^+$\;or\;Na^+ concentration were 20 mM or 25mM and the optimum $Mg^{2+}$ concentration was 10 mM. The enzyme activity was inhibited by N-ethyl-maleimide, aphidicolin, cytosine $\beta-D-arabinofuranoside$ 5'-triphoshate (ara CTP) and phosphonoacetic acid.

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A Case Study on Occurrence of Landslide by Heavy Rainfall in Hongcheon Area in 2006 (2006년 집중호우에 의한 홍천지역의 산사태 발생 사례 연구)

  • Kim,, Ho-Jin;Im, Oh-Bin;Yoo, Nam-Jae
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.03a
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    • pp.877-882
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    • 2010
  • This paper is a result of investigating causes and main characteristics of landslides, occurred at Hongcheon area in Gangwondo during July in 2006, by collecting relevant data and visiting site. The main cause of landslides in this area has been found to be saturation of the ground wetted by a series of precipitations during 10~13 July and the heavy rainfall during 15 July. The pattern of the landslides could be classified as translational failure, occurred at the boundary between the relatively thin weathered residual soil and the mother rock. By analyzing a number of failed slopes based on site visit and reviewing collected data, typical widths of failed slopes are in the range of 10~20m (minimum: 5m, maximum: 70m). Lengths of landslide area are in the wide range of 10~450m. Most of area are less than 20m in width and 100m in length so that their shapes are long and narrow, frequently observed in Korea, and their areas are relatively small size of around $1000m^2$. The inclinations of the failed slopes are in the range of $10{\sim}60^{\circ}$ while the most probable slope angle is about $20{\sim}25^{\circ}$.

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