• International Journal of Oral Biology
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• 제37권2호
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• pp.43-50
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• 2012

The use of high throughput screening (HTS) in drug development is principally for the selection new drug candidates or screening of chemical toxicants. This system minimizes the experimental environment and allows for the screening of candidates at the same time. Umbilical cord-derived stem cells have some of the characteristics of fetal stem cell and have several advantages such as the ease with which they can be obtained and lack of ethical issues. To establish a HTS system, optimized conditions that mimic typical cell culture conditions in a minimal space such as 96 well plates are needed for stem cell growth. We have thus established a novel HTS system using human umbilical cord derived-mesenchymal stem cells (hUC-MSCs). To determine the optimal cell number, hUC-MSCs were serially diluted and seeded at 750, 500, 200 and 100 cells per well on 96 well plates. The maintenance efficiencies of these dilutions were compared for 3, 7, 9, and 14 days. The fetal bovine serum (FBS) concentration (20, 10, 5 and 1%) and the cell numbers (750, 500 and 200 cells/well) were compared for 3, 5 and 7 days. In addition, we evaluated the optimal conditions for cell cycle block. These four independent optimization experiments were conducted using an MTT assay. In the results, the optimal conditions for a HTS system using hUC-MSCs were determined to be 300 cell/well cultured for 8 days with 1 or 5% FBS. In addition, we demonstrated that the optimal conditions for a cell cycle block in this culture system are 48 hours in the absence of FBS. In addition, we selected four types of novel small molecule candidates using our HTS system which demonstrates the feasibility if using hUC-MSCs for this type of screen. Moreover, the four candidate compounds can be tested for stem cell research application.

• 대한골관절종양학회지
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• 제13권2호
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• pp.67-74
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• 2007

목적: 골의 거대세포종양의 재발과 면역조직화학적 표지자와의 연관성을 연구하였다. 대상 및 방법: 골에서 유발된 거대세포종양 10례를 대상으로 하였다. 6명은 남자, 4명은 여자였다. 모든 환자는 수술 전 생검을 통해 확진 후 수술을 시행하였다. 방사선학적 분류는 Enneking grading system에 의하여 이루어졌다. 면역조직화학적 연구를 위해 MCM3, Ki-67 그리고 HH3 표지자가 사용되었다. 면역조직화학적 검사는 Microarray block을 사용하여 시행하였다. 결과: 10례 중 3례(30%)에서 같은 위치에서 재발되었다. 재발된 3례 중 2례는 방사선학적 단계 상 단계 2였고, 1례는 단계 1이었다. 면역조직화학적 표지자의 발현율이 방사선학적 단계 1보다 2, 3에서 증가되었다. 하지만 결과의 일관성이 없어 세포 증식율과 방사선학적 단계의 연관성은 판별하기 어렵다. 평균 MCM3 표지자의 발현율은 재발하지 않은 종양에서 11.2%, 재발한 종양에서 7.2%였다. Ki-67은 12%, 8.9% 였고, HH3는 66.9%, 75.4%였다. MCM3 와 Ki-67 표지자는 재발한 종양에서 오히려 감소된 결과를 보여 재발율과는 연관이 없을 것으로 생각된다. HH3표지자는 재발한 종양에서 증가된 소견을 보여 거대세포종양의 재발과 연관이 있음을 보여주었다. 결론: 본 연구는 면역조직화학적 표지자 중 HH3표지자가 거대세포종양의 재발 가능성을 판정하는데 기준이 될 수 있을 것으로 생각된다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2002년도 춘계종합학술대회
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• pp.257-260
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• 2002

차세대 블록 암호 표준인 AES(Advanced Encryption Standard) Rijndael(라인달) 암호 프로세서를 설계하였다. 라운드 변환블록 내부에 서브 파이프라인 단계를 삽입하여 현재 라운드의 후반부 연산과 다음 라운드의 전반부 연산이 동시에 처리되도록 하였으며, 이를 통하여 암.복호 처리율이 향상되도록 하였다. 라운드 처리부의 주요 블록들이 암호화와 복호화 과정에서 하드웨어 자원을 공유할 수 있도록 설계함으로써, 면적과 전력소비가 최소화되도록 하였다. 128-b/192-b/256-b의 마스터 키 길이에 대해 라운드 변환의 전반부 4 클록 주기에 on-the-fly 방식으로 라운드 키를 생성할 수 있는 효율적인 키 스케줄링 회로를 고안하였다. Verilog HDL로 모델링된 암호 프로세서는 Xilinx FPGA로 구현하여 정상 동작함을 확인하였다. 0.35-$\mu\textrm{m}$ CMOS 셀 라이브러리로 합성한 결과, 약 25,000개의 게이트로 구현되었으며, 2.5-V 전원전압에서 220-MHz 클록으로 동작하여 약 520-Mbits/sec의 성능을 갖는 것으로 예측되었다.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2008년도 추계학술대회 논문집
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• pp.206-208
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• 2008

In this paper, the two-stage DC-DC converter is proposed to make the control simple and to boost the low input voltage in the fuel cell generating system. The low efficiency of the conventional power converter is caused by a characteristic of the low-voltage and high-current in the fuel cell generating system. High-frequency transformer is needed to block the noise and to guarantee the safety of cell and load as a magnetically insulation. The proposed two-stage DC-DC converter for a fuel cell generation is more efficient than the traditional one-stage converter and easy to control. The design of a high-frequency transformer is also simple. Finally, the utility of the proposed converter is proved by the simulations and experiments.

• Macromolecular Research
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• 제12권6호
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• pp.545-552
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• 2004

The long-term stability of sulfonic acid-containing polyimides has been investigated. The hydrolytic degradation of homopolyimide and the block copolyimide comprising $27\;mol\%$ of 2,2'-bis(trifluoromethyl)benzidine and $9\;mol\%$ of m-phenylenediamine (BTFMB27mPl0[7/(3+1)]), was quantified through viscosity measurements and FT-IR spectroscopic analyses. The viscosity decrease with respect to time and the degradation rate were similar. The degrees of degradation with respect to time under ambient conditions and at elevated temperature in water were monitored by FT-IR spectroscopy. A new absorption peak was observed at $1786\;cm^{-1},$ which we corresponds to the presence of anhydride end groups formed by hydrolytic scission of the imide rings.

• 대한세포병리학회지
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• 제1권2호
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• pp.179-184
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• 1990

Bone metastasis of hepatocellular carcinoma appears to be peculiar when clinical manifestation of liver disease is not apparent, and initial diagnosis of metastatic hepatocellular carcinoma by fine needle aspiration cytology is rarely obtained. We experienced a case of 45-year-old man with metastatic hepatocellular carcinoma in the sacrum, which was diagnosed by fine needle aspiration cytology. The intrahepatic mass, measuring 1.2 cm in diameter and kept unchanged in size for two years, was never proved to be hepatocellular carcinoma histopathologically. The aspirated neoplastic cells were mostly in sheets, showing abundant acidophilic cytoplasm and large, round, centrally located nuclei with single, prominent acidophilic mucleoli. In the cell block section, diagnosis of metastatic well-differentiated hepatocellular carcinoma was made without difficulty, and definite trabecular fashion with sinusoidal endothelial cell lining was found.

• Clinical and Experimental Reproductive Medicine
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• 제23권1호
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• pp.1-6
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• 1996

There are a number of problems during the process of culture in vitro on fertilization and embryo development compared to those on in vivo counterparts. And the platelet activating factor (PAF), which is found not only in mammalian spermatozoa but also preembryos, is implicated on reproductive process. To improve the environment of culture on in vitro fertilization and embryo development, coculture using salpingeal epithelial cells has been considered to accept the better result on pregnancy rate. This study was designed to determine if two different culture systems, coculture alone and PAF treated coculture, are positive or negative influence on process of in vitro fertilization and embryo culture in mouse. The cell cleavage rate reached to 2-4 cell stage at 24 hours of culture is 56.81% (50/88) and 48.21%(54/112) respectively, in PAF treated group which is added PAF on coculture and in coculture group. But the rate of cells cleavage was similar in both group after 48 hours of culture. The rate of unfertilization after insemination of oocytes was higher in coculture group(55..53%) than in PAF treated group(42.37%). And in assessment of undeveped embryos, the rate of equalized cell block was similar on both, coculture alone (35.3%)and PAF treated coculture(35.5%). while unequalized cell block was higher rate in PAF treated coculture(19.4%) than coculture alone (11.8%). But the rate of cytoplasmic degeneration of undeveloped embryos was significantly higher in PAF treated coculture than coculture alone. In conclusion, we have observed that PAF treated coculture is superior in the rates of in vitro fertilization and early embryo cell cleavage compared to those in coculture alone, but there is no difference on the rates of embryo develpments, cell degeneration, cell quality in both PAF treated coculture and coculture alone when the embryo cells were continuosly cultured for 48 hours or more.

• 대한임상검사과학회지
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• 제45권2호
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• pp.66-72
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• 2013

Fine Needle Aspiration Biopsy Cytology (FNABC), which is known as the most accurate and cost-effective method for diagnosis of the thyroid nodule, may still result in indeterminate cases that are cellular paucity and show minor nuclear atypia. However, most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs which is currently helping us to differentiate malignancies from benign lesions. Cases studied included 46 histological, confirmed PTC cases. FNABC 102 cell paucity and 74 atypia benign cases were previously diagnosed as suspicious of PTC using cytologic examination. These cases were analyzed for BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme. In this study, the sensitivity and specificity were calculated and, BRAF mutation was detected by means of a histological method in 23 of 46 cases of PTC and no mutation was found in 22 cases. However, one case was not detected. In using FNABC, BRAF mutation was detected in 6 of 102 cases in cell paucity and in 11 of 74 cases in the atypia. Two cases were not detected in the atypia. The sensitivity and specificity of PCR-RFLP in FNABC were 60% and 97.4% respectively. Assessment of Formalin Fixed Paraffin Embedding (FFPE) block demonstrated similarly a 51.1% positive and 48.9% negative in PTC. Evaluation of BRAF mutation revealed high specificity and low sensitivity in using FNABC method. This study suggests that BRAF mutation analysis should be useful for the clinical diagnosis of PTC in FNABC with cytological findings suspicious for PTC.

• 한국정보통신학회논문지
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• 제15권6호
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• pp.1355-1362
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• 2011

본 논문에서는 IEEE 802.11n 무선 랜 표준에 규정된 3가지 블록길이(648, 1296, 1944)와 4가지 부호율(1/2, 2/3, 3/4, 5/6)을 지원하는 LDPC 복호기를 최소합 알고리듬과 layered 복호방식을 적용하여 설계하였다. 검사노드 값과 패리티 검사 행렬 정보의 효율적인 저장방법을 통해 메모리 용량을 최소화하였으며, 또한 효율적인 검사노드 메모리 어드레싱 방법을 적용하여 stall 없이 메모리 읽기/쓰기가 가능하도록 하였다. 설계된 회로는 FPGA 구현을 통해 하드웨어 동작을 검증하였으며, $0.18-{\mu}m$ CMOS 셀 라이브러리로 합성한 결과 219,100 게이트와 45,036 비트의 메모리로 구현되었고, 50 MHz@2.5V로 동작하여 164～212 Mbps의 성능을 갖는 것으로 평가되었다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2011년도 춘계학술대회
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• pp.132-135
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• 2011

본 논문에서는 IEEE 802.11n 무선 랜 표준의 3가지 블록길이(648, 1296, 1944)와 4가지 부호율(1/2, 2/3, 3/4, 5/6)을 지원하는 다중모드 LDPC 복호기를 설계하였다. 하드웨어 복잡도를 고려하여 layered 복호방식의 블록-시리얼(부분병렬) 구조로 설계하였으며, 최소합 알고리듬의 특징을 이용한 검사노드 메모리 최소화 방법을 고안하여 적용함으로써 기존방법에 비해 검사노드 메모리 용량을 약 47% 감소시켰다. 설계된 회로는 FPGA 구현을 통해 하드웨어 동작을 검증하였으며, $0.18-{\mu}m$ CMOS 셀 라이브러리로 합성한 결과 219,100 게이트와 45,036 비트의 메모리로 구현되었고, 50 MHz@2.5V로 동작하여 164~212 Mbps의 성능을 갖는 것으로 평가되었다.