• Biomolecules & Therapeutics
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• v.31 no.6
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• pp.682-691
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• 2023

Cell transformation induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol-13-acetate (TPA) is a critical event in cancer initiation and progression, and understanding the underlying mechanisms is essential for the development of new therapeutic strategies. Licorice extract contains various bioactive compounds, which have been reported to have anticancer and anti-inflammatory effects. This study investigated the cancer preventive efficacy of licochalcone D (LicoD), a chalcone derivative in licorice extract, in EGF and TPA-induced transformed skin keratinocyte cells. LicoD effectively suppressed EGF-induced cell proliferation and anchorage-independent colony growth. EGF and TPA promoted the S phase of cell cycle, while LicoD treatment caused G1 phase arrest and down-regulated cyclin D1 and up-regulated p21 expression associated with the G1 phase. LicoD also induced apoptosis and increased apoptosis-related proteins such as cleaved-caspase-3, cleaved-caspase-7, and Bax (Bcl2-associated X protein). We further investigated the effect of LicoD on the AKT signaling pathway involved in various cellular processes and found decreased p-AKT, p-GSK3β, and p-NFκB expression. Treatment with MK-2206, an AKT pharmacological inhibitor, suppressed EGF-induced cell proliferation and transformed colony growth. In conclusion, this study demonstrated the potential of LicoD as a preventive agent for skin carcinogenesis.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.37-45
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• 2007

The genetic transformation of watermelon by Agrobacterium has been known very difficult and a few successful cases have been reported by obtaining the direct shoot formation. However, since this direct shoot formation is not guaranteed the stable transformation, the stable transformation with reproducibility is required by a different approach such as a callus induced manner. The best conditions for inducing the callus from cotyledon and root explants of watermelon were 2 mg/L zeatin + 0.1 mg/L IAA and 2 mg/L BA + 0.1 mg/L 2,4-D, respectively. The GFP expression in the callus was identified and monitored through fluorescent microscopy after transformation with pmGFP5-ER vector. Paromomycin rather than kanamycin was used for selecting the nptll gene expression because it was more effective to select the watermelon explants. Four different callus types were observed and the solid green callus showed stronger GFP expression. The highest frequency of GFP expression in the callus developed from cotyledon was 9.0% (WM8 inbred line), while the highest frequency from root was 8.3% (WM6 inbred line). The WMV-CP was transformed using the method of GFP transformation and the genetic transformation of WMV-CP was confirmed by PCR and Southern blot analysis. Here we present a system for callus induction of watermelon explant and the callus induced method would facilitate the establishment of stable watermelon transformation.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.9
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• pp.911-916
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• 2005

In this study, variations of water quality, sediment size and contaminant substances are analyzed at upstream and downstream in sewer systems in order to evaluate the characteristics of wastewater transformation by physico-chemical and biological reactions. The differences of DOC concentration between up and down stream showed the range of $-5.8{\sim}18.6$ from the result of continuous measurement at up and down stream. About 8.4% of DOC concentration was reduced and reduction rate was 2.3 mg/L/km. SS reduction rate was measured by 5.5 mg/L/km, 0.22 mg/L/min from upstream to downstream, which was twice than DOC reduction rate. When pollution load reduction was evaluated considering infiltration/inflow effect, DOC load was eliminated from 1,230 ka/d to 1,167 kg/d by physi-chemical and biological reaction in a sewer and 7.8% of the SS in upstream station was reduced under dry weather condition. The results showed that the characteristics of transformation process of wastewater in sewer should be considered for design and operation of wastewater treatment plant.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.05a
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• pp.602-607
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• 2002

In this paper, we proposed an approach to efficiently compress and transmit multiresoltion 3D lariat models for multimedia and very low bit rate applications. A personal facial model is obtained by a 3D laser digitizer, and further re-quantized at several resolutions according to different scope of applications, such as animation, video game, and video conference. By deforming 2D templates to match and re-quantize a 3D digitized facial model, we obtain its compressed model. In the present study, we create hierarchical 2D lariat wireframe templates are adapted according to facial feature points and the proposed piecewise chainlet affined transformation(PACT) method. The 3D digitized model after requantization are reduced significantly without perceptual loss. Moreover the proposed multiresoulation lariat models possessed of hierarchial data structure are apt to be progressively transmitted and displayed across internet.

• Journal of Plant Biotechnology
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• v.2 no.2
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• pp.89-96
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• 2000

For large DNA fragment transformation in dicots and monocots, BIBAC2 vector system was applied to Arabidopsis thaliana and Oryza sativa L. cv. Jinmi as a model plant, respectively. For Arabidopsis, the Th1 gene in T23L3 BAC clone whose size is about 90 kb was used as the target gene source for transformation. Because T23L3 BAC clone was originally constructed in pBelloBAC11, the target gene was reconstructed into BIBAC2. As the results of reconstruction, 476 colonies were survived in selection medium containing 40 mg/L kanamycin. In colony hybridization analysis, 24 out of 476 colonies exhibited positive signals. In the pulsed-field gel electrophoresis analysis, 11 out of 24 positive clones exhibited the band at the location of 90 kb. In Southern hybridization, positive signal band at the location of 90 kb was observed in all 11 transformants. Using these verified clones, Agrobacterium-mediated transformation was applied to Arabidopsis thaliana th1-201 mutant for genetic complementation test. Twelve thousands T$_1$ seeds were harvested, and antibiotic selection test is being analyzed to verify whether these seeds were transformed. for rice, COR356 that contains 150 kb human genomic DNA in a BIBAC2 vector was used as the target gene. As the results of transformation, 151 out of 210 co-cultivated calli were survived in selection medium containing 5 mg/L hygromycin, and 45 out of 151 survived calli were regenerated into plants. Transformation efficiency was 21.6%. Progeny test using 71 seeds is being analyzed now. These results provide the potential that large DNA fragments can be transferred into both dicots and monocot by Agrobacterium-mediate d transformation system.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.27 no.2
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• pp.281-288
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• 2009

In the paper, we try to develop the methodology for updating road networks of large-scale digital maps by using construction drawing data. For the purpose, it is pre-requite step to merge road networks detached in CAD drawing data. As such, tie points are identified in neighboring drawings and used for solving the parameters of 2D conformal transformation between drawings. Then, the merged road network in CAD data is transformed to the coordinate system of digital maps. In the process, IPs in the drawings are considered as control information and 2D affine transformation is selected for coordinate transformation. Through the experiments with real dataset, we can identify that the developed method is valid and generally applicable.

• Asian Journal of Innovation and Policy
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• v.1 no.2
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• pp.133-147
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• 2012

Asia is becoming a stronghold for innovation careers along with its resurgence in economic, political, and cultural arenas. This transformation happened on the backdrop of growing enrolments in higher education, increasing investments in scientific research & innovation, and innovation supportive policy measures by several Asian countries. Research and Development (R&D) and product customization centres of multinational corporations (MNCs) sited in emerging nations are also instrumental in this transformation.

• Bulletin of the Korean Chemical Society
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• v.30 no.3
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• pp.695-699
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• 2009

A short and efficient synthesis of (2S,3S)-safingol from commercially available D-ribo-(2S,3S,4R)-phytosphingosine is described. The highlights of the synthesis are a stereoselective one-pot transformation of a diol into an epoxide under phase transfer catalytic conditions and a regioselective epoxide-opening reduction with a hydride reagent.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.393-396
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• 2005

The reactive transport model on the biologically mediated sequential nitrate transformation and its subsequent transport was developed and tested. This model was coded as a reaction module within the RT3D framework (Clement, 1997). Transports of the reasonable six mobile solutes (dissolved organic carbon, $O_2,\;{NO_3}^-,\;{NO_2}^-,\;N_2O,\;N_2$) and two immobile microbes were simulated. The simulation results gave a reasonable match with supposed transport pattern. For the next step, the developed model will be validated against experimental data.

• Journal of electromagnetic engineering and science
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• v.14 no.2
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• pp.47-53
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• 2014

This paper presents a high gain and high harmonic rejection low-noise amplifier (LNA) for software-defined radio receiver. This LNA exploits the high quality factor (Q) series resonance technique. High Q series resonance can amplify the in-band signal voltage and attenuate the out-band signals. This is achieved by a source impedance transformation. This technique does not consume power and can easily support multiband operation. The chip is fabricated in a $0.13-{\mu}m$ CMOS. It supports four bands (640, 710, 830, and 1,070MHz). The measured forward gain ($S_{21}$) is between 12.1 and 17.4 dB and the noise figure is between 2.7 and 3.3 dB. The IIP3 measures between -5.7 and -10.8 dBm, and the third harmonic rejection ratios are more than 30 dB. The LNA consumes 9.6 mW from a 1.2-V supply.