• Title/Summary/Keyword: 2-D color distribution

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Significance of Urease Distribution across Helicobacter pylori Membrane

  • Gang, Jin-Gu;Yun, Soon-Kyu;Choi, Kyung-Min;Lim, Wang-Jin;Park, Jeong-Kyu;Hwang, Se-Young
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.317-325
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    • 2001
  • For heuristic purposes, the relative ratio of urease contents inside and outside cells was surveyed using nine ureB+ strains of Helicobacter pylori. the ratio of the enzyme specific activity appeared to vary greatly between the various H. pylori strains, ranging from 0.5 to 2.5. Besides the above compartment, urease was also richly found in the membrane fraction, especially in either peripheral or integral form. The urease distribution across the H. pylori membrane was significantly influenced by the ambient pH; the specific activity of external urease was highest at pH 5.5 with a narrow plateau, whereas the internal specific activity was highest within a pH range of 4.5 to 6.5 with a broad plateau. These finding strongly suggest that H. pylori urease is secretory and responded to the external pH. However, at pH 4.0 or below, no urease activity was detected in either the internal or external compartment, although an increase in the color development with 2,4,6-trinitrobenzene sulfonate (TNBS) was observed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that these phenomena may be related to a specific proteolysis in certain proteins, including urease or ${\gamma}$-glutamyl transpeptidase. Interestingly, the effect of ammonium ions n alleviating the enzyme inactivation inside the H. pylori cells was remarkably similar to that of D-glucose. In addition, it would appear that the cation acted as a surrogate of not only $Na^+$ but also $K^+$ thereby increasing the H. pylori P-type ATPase activity. This is of great interest, as it implies that the urease action in H. pylori is indispensible at any locus.

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Quality characteristics and sensory evaluation of Fuji apple based on commodity price (상품 가격에 따른 사과의 품질 특성 및 관능 평가)

  • Ku, Kyung Hyung;Choi, Eun Jeong;Kim, Sang-Seop;Jeong, Moon Cheol
    • Food Science and Preservation
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    • v.23 no.7
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    • pp.1065-1073
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    • 2016
  • This study investigated the sensory attributes and quality characteristics of Fuji apples based on market commodity price to provide data for quality index of Fuji apples. Samples were purchased from the Garak market (Seoul Agro-Fisheries & Food Corporation) and divided into four groups depending on the price such as group A, B, C, D. There were no significant differences in their volume and weight among groups. In the soluble solid content and total free sugar, A and B group (high price) showed higher content than those of C and D (low price) group. And also, the A group and B, C, D group showed 386.29 mg% and 320.09~359.28 mg% in the total organic acid content, respectively. As an sensory evaluation results, A group and B group were evaluated higher score than those of C and D group in the uniformity of red color and glossiness of skin and unique apple sensory attributes using quantitative descriptive analysis. Consumer test showed similar to quantitative descriptive analysis results in the various sensory attributes. In the analysis results between quality characteristics and sensory attributes of Fuji apples, total acceptability was correlated positively with titratable acidity (r=0.58), soluble solid (r=0.89), soluble solid content/titratable acidity ratio (r=0.42), total free sugar (r=0.36) and total organic acid (r=0.38). Based on principal component analysis of apple's quality characteristics, apples were primary separated along the first principal component (pH, acidity, soluble solid content, total free sugar, organic acid), which accounted for 66.01% of total variance. In addition, principal component analysis of sensory evaluation revealed a total variance for the quantitative descriptive of 55. 65% and a total variance for the consumer test of 55.84%.

Spatial and Temporal Aspects of Phytoplankton Blooms in Complex Ecosystems Off the Korean Coast from Satellite Ocean Color Observations

  • Ahn, Yu-Hwan;Shanmugam, Palanisamy;Chang, Kyung-Il;Moon, Jeong-Eon;Ryu, Joo-Hyung
    • Ocean Science Journal
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    • v.40 no.2
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    • pp.67-78
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    • 2005
  • Complex physical, chemical and biological interactions off the Korean coast created several striking patterns in the phytoplankton blooms, which became conspicuous during the measurements of ocean color from space. This study concentrated on analyzing the spatial and temporal aspects of phytoplankton chlorophyll variability in these areas using an integrated dataset from a Sea-viewing Wide Field-of-view Sensor (SeaWiFS), Advanced Very High Resolution (AVHRR) sensor, and Conductivity Temperature Depth (CTD) sensor. The results showed that chlorophyll concentrations were elevated in coastal and open ocean regions, with strong summer and fall blooms, which appeared to spread out in most of the enclosed bays and neighboring waters due to certain oceanographic processes. The chlorophyll concentration was observed to range between 3 and $54\;mg\;m^{-3}$ inside Jin-hae Bay and adjacent coastal bays and 0.5 and $8\;mg\;m^{-3}$ in the southeast sea offshore waters, this gradual decrease towards oceanic waters suggested physical transports of phytoplankton blooms from the shallow shelves to slope waters through the influence of the Tsushima Warm Current (TWC) along the Tsushima Strait. Horizontal distribution of potential temperature $(\theta)$ and salinity (S) of water off the southeastern coast exhibited cold and low saline surface water $(\theta and warm and high saline subsurface water $({\theta}>12^{\circ}C; S>34.4)$ at 75dBar, corroborating TWC intrusion along the Tsushima Strait. An eastward branch of this current was called the East Korean Warm Current (EKWC), tracked with the help of CTD data and satellite-derived sea surface temperature, which often influenced the dynamics of mesoscale anticyclonic eddy fields off the Korean east coast during the summer season. The process of such mesoscale anticyclonic eddy features might have produced interior upwelling that could have shoaled and steepened the nutricline, enhancing phytoplankton population by advection or diffusion of nutrients in the vicinity of Ulleungdo in the East Sea.

An Analysis of Luminance and Chromaticity on Outdoor Lighting of Historic Buildings -focused on Namdaemun and Kwanghwamun- (휘도와 색도측면에서 본 역사적 건축물의 야간경관조명 분석 -남대문과 광화문을 중심으로-)

  • 안현태;김정태
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.15 no.1
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    • pp.36-43
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    • 2001
  • Recently, outdoor lighting of buildings has bemIre activated in national level of Korea. Therefore, this study aims to analyze the outdoor ljghting effect of historic buildings with particular reference to two traditional gates architecture - Namdaemtul and Kwanghwamun For the purpose, luminance and chromaticity of outdoor lighting are measured. The results of the study are as follows; $\circled1$ Outdoor lighting of the historic buildings were mainly illuminated by the floodlights. In addition, up-lighting and accent lighting were locally used. $\circled2$ Basements and rooflines were specially emphasized, and they represented the night image of historical buildings. $\circled3$ Chromaticity distribution of historical building was wlute during the day. However, it was yellow dL1Jing the night due to the color and color temperature of outdoor lighting.ghting.

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An Investigation on the Brown Rot of Eggplant Caused by Phytophthora capsici Leonian (Phytophthora capsici Leonian균에 의한 가지의 갈색썩음병에 관한 조사)

  • Kim Byung Soo;Lee Eung Kwon;Chung Bong Koo
    • Korean journal of applied entomology
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    • v.14 no.2 s.23
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    • pp.77-79
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    • 1975
  • Brown rot of eggplant caused by Phytophthora capsici has not been reported so far in Korea. The wide distribution of the disease was found at the central Korea including Suweon. The symptom of the disease was characterized by water sealing lesions at first, the color gradually turn brown and a little sunken area on the fruit appearred at the final stage. Dense hyphae and short sporangiophores grew out from the lesions, and they produce abundant zoosporangia on them. The disease was identified from buck-eye rot caused by Phytophthora parasitica in that the cottony growth of hyphae and rare zoosporangia formation. The pathogen was isolated and indentified on the basis of the pathogenecity and morphology comparing with P. parasitica.

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The Effect of Air and Spray Turbulence on the Progress in a D.I. Diesel Engine(II)-Combustion Chamber Design for the Use of Emulsified Diesel Oil with Water Particles- (직접분사식 디젤기관의 연소실 형상과 화염의 발달 (2)-유화액연료용 연소실의 형상-)

  • ;;Ohta, Motoo
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.11
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    • pp.3054-3062
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    • 1995
  • Recently, the improvement of fuel economy and the reduction of exhaust smoke and NOx have been successfully achieved by supplying diesel engines with emulsified diesel oil with water particles. In the present paper, the difference between the combustion of injected emulsified fuel and that of diesel oil spray is clarified by means of taking high-speed and color photographs of the flames in the engine cylinder. As the results, the two kinds of fuels show different combustion behavior each other in the growth of initial flame and in the termination of combustion process in the cylinder. Then, suitable combustion chamber design for the use of emulsified fuel is discussed on the basis of experimental data for various distribution of spray in different kinds of piston cavities. Some methods of clearing troubles caused by emulsified fuel injection are also discussed on the basis of performance tests with a remodeling test engine.

Improved Flowability and Wettability of Whey Protein-Fortified Skim Milk Powder via Fluidized Bed Agglomeration

  • Seo, Chan Won
    • Food Science of Animal Resources
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    • v.42 no.6
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    • pp.915-927
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    • 2022
  • Recently, protein-fortified milk powders are being widely consumed in Korea to prevent sarcopenia, and the demand for high-protein food powders is continuously increasing in the Korean market. However, spray-dried milk proteins have poor flowability and wettability owing to their fine particle sizes and high inter-particle cohesive forces. Fluidized bed agglomeration is widely used to improve the instant properties of food powders. This study investigated the effect of fluidized bed agglomeration on whey protein isolate (WPI)-fortified skim milk powder (SMP) at different SMP/WPI ratios. The fluidized bed process increased the particle size distribution, and agglomerated particles with grape-like structures were observed in the SEM images. As the size increased, the Carr index (CI) and Hausner ratio (HR) values of the agglomerated WPI-fortified SMP particles exhibited excellent flowability (CI: <15) and low cohesiveness (HR: <1.2). In addition, agglomerated WPI-fortified SMP particles exhibited the faster wetting time than the instant criterion (<20 s). As a result, the rheological and physical properties of the WPI-fortified SMP particles were effectively improved by fluidized bed agglomeration. However, the fluidized bed agglomeration process led to a slight change in the color properties. The CIE L* decreased, and the CIE b* increased because of the Maillard reaction. The apparent viscosity (ηa,10) and consistency index (K) values of the rehydrated solutions (60 g/180 mL water) increased with the increasing WPI ratio. These results may be useful for formulating protein-fortified milk powder with better instant properties.

Skin Pigmentation Detection Using Projection Transformed Block Coefficient (투영 변환 블록 계수를 이용한 피부 색소 침착 검출)

  • Liu, Yang;Lee, Suk-Hwan;Kwon, Seong-Geun;Kwon, Ki-Ryong
    • Journal of Korea Multimedia Society
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    • v.16 no.9
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    • pp.1044-1056
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    • 2013
  • This paper presents an approach for detecting and measuring human skin pigmentation. In the proposed scheme, we extract a skin area by a GMM-EM clustering based skin color model that is estimated from the statistical analysis of training images and remove tiny noises through the morphology processing. A skin area is decomposed into two components of hemoglobin and melanin by an independent component analysis (ICA) algorithm. Then, we calculate the intensities of hemoglobin and melanin by using the projection transformed block coefficient and determine the existence of skin pigmentation according to the global and local distribution of two intensities. Furthermore, we measure the area and density of the detected skin pigmentation. Experimental results verified that our scheme can both detect the skin pigmentation and measure the quantity of that and also our scheme takes less time because of the location histogram.

Evaluation of the stress distribution in the external hexagon implant system with different hexagon height by FEM-3D (임플란트 hexagon 높이에 따른 임플란트와 주위 조직의 응력분포 평가)

  • Park, Seong-Jae;Kim, Joo-Hyeun;Kim, So-Yeun;Yun, Mi-Jung;Ko, Sok-Min;Huh, Jung-Bo
    • The Journal of Korean Academy of Prosthodontics
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    • v.50 no.1
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    • pp.36-43
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    • 2012
  • Purpose: To analyze the stress distribution of the implant and its supporting structures through 3D finite elements analysis for implants with different hexagon heights and to make the assessment of the mechanical stability and the effect of the elements. Materials and methods: Infinite elements modeling with CAD data was designed. The modeling was done as follows; an external connection type ${\phi}4.0mm{\times}11.5mm$ Osstem$^{(R)}$ USII (Osstem Co., Pusan, Korea) implant system was used, the implant was planted in the mandibular first molar region with appropriate prosthetic restoration, the hexagon (implant fixture's external connection) height of 0.0, 0.7, 1.2, and 1.5 mm were applied. ABAQUS 6.4 (ABAQUS, Inc., Providence, USA) was used to calculate the stress value. The force distribution via color distribution on each experimental group's implant fixture and titanium screw was studied based on the equivalent stress (von Mises stress). The maximum stress level of each element (crown, implant screw, implant fixture, cortical bone and cancellous bone) was compared. Results: The hexagonal height of the implant with external connection had an influence on the stress distribution of the fixture, screw and upper prosthesis and the surrounding supporting bone. As the hexagon height increased, the stress was well distributed and there was a decrease in the maximum stress value. If the height of the hexagon reached over 1.2mm, there was no significant influence on the stress distribution. Conclusion: For implants with external connections, a hexagon is vital for stress distribution. As the height of the hexagon increased, the more effective stress distribution was observed.

Articulated Human Body Tracking Using Belief Propagation with Disparity Map (신뢰 전파와 디스패리티 맵을 사용한 다관절체 사람 추적)

  • Yoon, Kwang-Jin;Kim, Tae-Yong
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.49 no.3
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    • pp.51-59
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    • 2012
  • This paper suggests an efficient method which tracks articulated human body modeled with markov network using disparity map derived from stereo images. The conventional methods which only use color information to calculate likelihood for energy function tend to fail when background has same colors with objects or appearances of object are changed during the movement. In this paper, we present a method evaluating likelihood with both disparity information and color information to find human body parts. Since the human body part are cylinder projected to rectangles in 2D image plane, we use the properties of distribution of disparity of those rectangles that do not have discontinuous distribution. In addition to that we suggest a conditional-messages-update that is able to reduce unnecessary message update of belief propagation. Since the message update has comprised over 80% of the whole computation in belief propagation, the conditional-message-update yields 9~45% of improvements of computational time. Furthermore, we also propose an another speed up method called three dimensional dynamic models assumed the body motion is continuous. The experiment results show that the proposed method reduces the computational time as well as it increases tracking accuracy.