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Genetic Polymorphism of Epoxide Hydrolase and GSTM1 in Chronic Obstructive Pulmonary Disease (만성폐쇄성폐질환 발생에 Epoxide hydrolase와 GSTM1유전자 다형성의 의의)

  • Park, Sang Sun;Kim, Eun Joung;Son, Chang Young;Wi, Jeong Ook;Park, Kyung Hwa;Cho, Gye Jung;Ju, Jin Young;Kim, Kyu Sik;Kim, Yu II;Lim, Sung Chul;Kim, Young Chul;Park, Kyung Ok;Na, Kook Joo
    • Tuberculosis and Respiratory Diseases
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    • v.55 no.1
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    • pp.88-97
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    • 2003
  • Background : Although smoking is a major cause of chronic obstructive pulmonary disease (COPD), only 10-20% of cigarette smokers develop symptomatic COPD, which suggests the presence of genetic susceptibility. This genetic susceptibility to COPD might depend on variations in the activities of the enzyme that detoxify hazardous chemical products, such as microsomal epoxide hydrolase (mEPHX) and glutathione-S transferase M1 subunit (GSTM1) genes. Methods : The genotypes of 58 patients with COPD, and 79 age matched control subjects, were determined by a polymerase chain reaction, followed by restriction fragment length polymorphism (PCR-RFLP) for the mEPHX, and multiplex PCR for the GSTM1. Results : GSTM1 was deleted in 53.3% of the subjects. There was no difference in GSTM1 deletion rates between the COPD patients (32/58, 55.2%) and the control subjects (41/79, 51.9%). The combination patterns of two polymorphisms of mEPHX showed slow enzyme activity in 29(21.2%), normal in 73(53.3%) and fast in 32(23.4%). The COPD group (7/57, 12.3%) showed a significantly lower incidence of slow enzyme activity compared to the control subjects (22/77, 28.6%, p<0.05). However, when the COPD and control groups were compared with smokers only, there were no significant differences in the genotypes of GSTM1 and mEPHX. Conclusion : The genotypes of GSTM1 and mEPHX were not significant risk factors of COPD in this cohort of study.

Locates the Sunken Ship 'Dmitri Donskoi' using Marine Geophysical Survey Techniques in Deep Water (지구물리 탐사기법을 이용한 심해 Dmitri Donskoi호 확인)

  • Yoo, Hai-Soo;Kim, Su-Jeong;Park, Dong-Won
    • 한국지구물리탐사학회:학술대회논문집
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    • 2004.08a
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    • pp.104-117
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    • 2004
  • Dmitri Donskoi, which went down during the Russo-Japanese War occurred 100 years ago, was found by using geophysical exploration techniques at the 400 m water depth of submarine valley off Jeodong of Ulleung Island. In the submarine area with the rugged seabed topography and volcanic seamounts, in particular, the reliable seabed images were acquired by using the mid-to-shallow Multibeam exploration technique The strength of corrosion (causticity) of the sunken Donskoi, measured by the electrochemical method, decreased to 2/5 compared with the original strength.

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Outcomes of the Modified Maze Procedure for Chronic Atrial Fibrillation Combined with Rheumatic Mitral Valve Disease (류마티스성 승모판막질환과 동반된 만성 심방세동 치료에 대한 변형 Maze 술식의 결과)

  • Baek Man-Jong;Kim Jae-Hyun;Seo Hong-Joo;Lee Chang-Ha;Oh Sam-Se;Na Chan-Young
    • Journal of Chest Surgery
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    • v.39 no.9 s.266
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    • pp.681-691
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    • 2006
  • Background: The aim of this study was to investigate the mid-term outcomes of our modifications to the maze procedure using cryoablation for treating atrial fibrillation associated with rheumatic mitral valve disease. Material and Method: Between March 2000 and February 2004, 177 consecutive patients underwent the modified maze procedure with the use of cryoablation concomitant with mitral valve surgery for atrial fibrillation associated with rheumatic mitral valve disease, and were divided into three groups: (1) modified Cox-maze III (CM group, n=88): (2) modified Kosakai-maze (KM group, n=63): and (3) left atrial maze procedure (LA group, n=26). The postoperative and follow-up results were analyzed and compared between the groups. Result: There were three hospital deaths (1.7%) and no significant differences in the incidence of postoperative complications between the three groups. The operative time, such as the cardiopulmonary bypass and aortic crossclamp time, were significantly longer in the CM group than in the KM and LA groups, respectively (p<0.0001). The mean follow-up was $22.4{\pm}15.1$ months ($1\sim52.6$ months) for all patients. One late death developed in the CM group (0.0%). At last follow-up, 139 patients exhibited sinus rhythm (79.9%), which was also regained in 67 patients (77.9%) in the CM group, 50 (80.7%) in the KM group and 22 (84.6%) in the LA group (p=0.743). The actuarial freedom from stroke at 4 years was $84.5{\pm}9.4%$ in the CM group, $95.0{\pm}4.9%$ in the KM group, and $92.9{\pm}6.9%$ in the LA group (p=0.916). Conclusion: The modified maze procedure using cryoablation is safe and effective in treating chronic atrial fibrillation associated with rheumatic mitral valve disease.

Relationship Between Foodservice Satisfaction and Customer Loyalty in Industry Contracted Foodservice (위탁급식 전문업체 산업체급식소의 고객 만족과 충성도와의 관계)

  • Kim, Hyeon-Ju;Lyu, Eun-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.8
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    • pp.1318-1324
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    • 2013
  • The purpose of this study was to improve the satisfaction of industry contracted foodservice customers by analyzing the correlation between satisfaction with foodservice and customer loyalty. To accomplish this, a questionnaire was distributed to 300 customers at five industry contracted foodservice establishments. The average customer satisfaction score was 3.51/5.00, and customer satisfaction was highest with sanitation, followed by service, food, and environment. Blue collar workers showed significantly higher foodservice satisfaction than white collar workers with respect to menu, service, sanitation, and environment (P<0.01). The average customer loyalty score was 3.46/5.00 and revisit intentions, word-of-mouth intentions, and intentions not to switch were 3.60, 3.52, and 3.46, respectively. Blue collar workers had significantly higher customer loyalty than white collar workers for word-of mouth intentions and intentions not to switch (P<0.01). Revisit intentions correlated significantly (P<0.01) with food (r=0.649), service (r=0.636), sanitation (r=0.648), and environment (r=0.579). Word-of mouth intentions and intentions not to switch showed significant (P<0.01) positive correlation with service. The explanation power ($R^2$) of foodservice satisfaction and revisit intention was 0.483, word-of-mouth intention was 0.526, and intention not to switch was 0.3641. The sanitation satisfaction had the highest positive influence on revisit intentions (P<0.01). Service and food satisfaction had a significant (P<0.01) positive influence on word-of-mouth intentions and intentions not to switch.

Changes of Blood Gases, Plasma Catecholamine Concentrations and Hemodynamic Data in Anesthetized Dogs during Graded Hypoxia Induced by Nitrous Oxide (아산화질소에 의한 점진적 저산소가스 흡입이 혈중 가스치와 Catecholamine치 및 혈역학에 미치는 영향)

  • Kim, Sae-Yeon;Song, Sun-Ok;Bae, Jung-In;Cheun, Jae-Kyu;Bae, Jae-Hoon
    • Journal of Yeungnam Medical Science
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    • v.15 no.1
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    • pp.97-113
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    • 1998
  • The sympathoadrenal system plays an important role in homeostasis in widely varing external environments. Conflicting findings, however, have been reported on its response to hypoxia. We investigated the effect of hypoxia on the sympathoadrenal system in dogs under halothane anesthesia by measuring levels of circulating catecholamines in response to graded hypoxia. Ten healthy mongreal dogs were mechanically ventilated with different hypoxic gas mixtures. Graded hypoxia and reoxygenation were induced by progressively decreasing the oxygen fraction in the inhalation gas mixture from 21%(control) to 15%, 10% and 5% at every 5 minutes, and then reoxygenated with 60% oxygen. Mean arterial pressure, central venous pressure and mean pulmonary arterial pressure were measured directly using pressure transducers. Cardiac output was measured by the thermodilutional method. For analysis of blood gas, saturation and content, arterial and mixed venous blood were sampled via the femoral and pulmonary artery at the end of each hypoxic condition. The concentration of plasma catecholamines was determined by radioenzymatic assay. According to the exposure of graded hypoxia, not only did arterial and mixed venous oxygen tension decreased markedly at 10% and 5% oxygen, but also arterial and mixed venous oxygen saturation decreased significantly. An increased trend of the oxygen extraction ratio was seen during graded hypoxia. Cardiac output, mean arterial pressure and systemic vascular resistance were unchanged or increased slightly. Pulmonary arterial pressure(PAP) and pulmonary vascular resistance(PVR) were increased by 55%, 76% in 10% oxygen and by 82%, 95% in 5% oxygen, respectively(p<0.01). The concentrations of plasma norepinephrine, epinephrine and dopamine increased by 75%, 29%, 24% in 15% oxygen and by 382%, 350%, 49% in 5% oxygen. These data suggest that the sympathetic nervous system was activated to maintain homeostasis by modifying blood flow distribution to improve oxygen delivery to tissues by hypoxia, but hemodynamic changes might be blunted by high concentration of nitrous oxide except PAP and PVR. It would be suggested that hemodynamic changes might not be sensitive index during hypoxia induced by high concentration of nitrous oxide exposure.

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On the Study of Forest Sampling Methods in Natural Deciduous Forest (활엽수림(闊葉樹林)에 대(對)한 자원조사법(資源調査法)의 연구(硏究))

  • Kim, Kap Duk
    • Journal of Korean Society of Forest Science
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    • v.17 no.1
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    • pp.35-42
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    • 1973
  • Deciduous trees grown naturally in the forest of Korea usually have an irregular Lee-form and their utility has been decreased. In Korea, most of the deciduous stands are distributed in the hinterland. A shortage of the total growing stock made utilization of them necessary in this country even though some difficulties to be cutted and transported are accompanied. Therefore, this study was conducted to select the suitable sampling method for surveying them. The results investigated are as follows. 1. Three locations being 10 hectares in each location for the plots were chosen and surveyed with six $20m{\times}50m$ rectangular sample plots by four types of sampling method below. And the result is shown in Table 1. A. Random sampling by grids B. Random sampling by co-ordinates C. Systematic line plot. D. Sub-sampling 2. One hundred and fifty hectares from all plots were sectioned through the application of aerial photography scaled of 1 : 15,000. The author divided forest types according to diameter class and crown density with mirror stereoscope. The forest types were divided into three classes. Seven sample plots from the area of 150 hectars are systematically arranged and the results investigated on the circular sample point of 0.1 hectare are shown in Table 4. 3. There were no significant differences between results by sampling method and by diameter measurement method (population mean) as shown in Table 3. 4. Random sampling by grid and systematic line plot are better than others. 5. There are more over-estimated values when the circular sample point is used than where the rectangular sample point. 6. As forest stands are irregular, smaller number of sample points will make many errors.

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A Study on the Children's Eating Habits and Food Preference according to Their Parents' Economic Status (I) - Seoul & Gyeonggi (Incheon) Area - (부모의 경제수준에 따른 자녀의 식습관과 식품기호도에 관한 연구 (I) -서울.경기 (인천)지역을 중심으로-)

  • Chung, Hea-Jung;Eum, Yun-Ho;Kim, Jung-Yoon
    • Journal of Nutrition and Health
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    • v.41 no.1
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    • pp.77-88
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    • 2008
  • This research was carried out a to investigate the food habit and preference of parents' social and economic level with 681 children (145 of Kindergarten, 300 of Children, 236 of Adolescences) in Seoul and Gyeonggi area using question naires. The parents' income level divided into 2 groups, less than 4 million won and equal or greater than 4 million won. Most of the middle years were over-weighing but kindergarten and adolescences had standard weights. The majority of parents had Bachelor degrees, most of father were office workers, majority of mothers of kindergarten with parents' income level less than 4 million won were professional women and mothers of the rest of the groups were mostly house wives. The middle years with parents income level less than 4 million son were skipping meals most frequently, but other groups didn't show much differences in their eating habits. All age groups showed that they like meats the most and dislike vegetables the worst. All age groups also showed that they mostly eat out 1-2 times a week and the next was 3-4 times a week. All age groups preferred ice creams, fruits, juices and snacks for their desserts. The middle years with parents' income equal or greater than 4 million won also showed high preference on strawberry and chocolate flavored milk, burgers and pizzas. The food s that preferred to eat when dining out were Chinese foods (Ja-jang-myeon and sweet and sour pork) for kindergarten group, Korean foods (kalbi and bulgogi) for the middle years with parents' income level less than 4 million won, family restaurant food (steak and rib) for the middle years with parents' income level equal or greater than 4 million won and Korean foods (kalbi and bulgogi) for the adolescences. The preferred cooking methods were roast (fish and sea weeds) for the kindergarten, roasted meat for the middle years, Kimchi and bean paste pot stew for the adolescences with parents' income level less than 4 million won and roasted meats for the adolescences with parents' income level equal or greater than 4 million won. The results showed that the adolescences with higher parental income lever preferred meats.

Serological Analysis and Epidemiologic Characteristics of Group A Streptococci in Seoul(1998-2000) (3년간(1998-2000년) A군 연구균 감염의 혈청학적 형분류와 역학적 특징)

  • Choi, Sun-Hee;Kim, Yun-Ho;Cha, Sung-Ho;Kim, Ki-Sang;Lee, Young-Hee
    • Clinical and Experimental Pediatrics
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    • v.45 no.11
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    • pp.1368-1372
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    • 2002
  • Purpose : Group A streptococci have a cell wall which consists of M protein and T protein. T protein is known to be helpful in the understanding of the epidemiology of group A streptococci. To study the epidemiologic characteristics, we serotyped T protein of group A streptococci obtained from patients admitted to hospitals, or who visited OPD in five districts of Seoul the during last three years. Methods : Group A streptococci were obtained in five districts in north, northeast, central, northwest and south Seoul from 1998 through 2000. All isolated group A streptococci were serotyped with T protein antisera(Institute of Sera and Vaccine, Prague, Czech Republic). Results : In 1998, analysis of obtained total number of 92 strains revealed that T12, T4, and NT acounted for 72.2%. Among seven cases of scarlet fever, T12 was isolated in four cases and T4 was found in three cases. Two cases of tonsilar abscess produced T8 and NT. One case of cervical lymphadenitis showed T12. In 1999, 41 cases were studied showing that T12, T4, and T1 contributed 68%. Among five cases of scarlet fever, T12 and T4 make up three case. There were two cases of pneumonia(T4 and T1) and one case of cervical lymphadenitis(T8/25). In 2000, the study was performed in four districts except the central area. Among 83 isolates, T12, T4 and T1 accounted for 63.9%. There were three cases of scarlet fever(T12, T4, T5), one case of tonsillar abscess(T12), one case of pneumonia(NT) and one case of sepsis(T1). Conclusion : Serological analysis of T protein of group A streptococci shows no endemic specificity. The yearly pattern reveals that T12 had been decreasing but T1 had shown the opposite trend.

Effects of Gamma Irradiation on Color Changes and Biological Activities of Ethanol Extract of a Mechanically Pressed Juice of Bokbunja (Rubus coreanus Miq.) (감마선 조사에 의한 복분자 착즙액 에탄올 추출물의 색상 및 생리활성 변화)

  • Kim, Hee-Jung;Jo, Cheor-Un;Kim, Hyun-Joo;Shin, Dong-Hwa;Son, Jun-Ho;Byun, Myung-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.3
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    • pp.271-277
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    • 2006
  • A mechanically pressed juice of Bokbunja (Rubus coreanus Miq.) extract was prepared using 70% ethanol solution. The extract was subjected to gamma-irradiation treatment (20 kGy) and investigated for its change of color and biological activities. Hunter $L^*$ values of the irradiated Bokbunja extract were increased in comparison with the non-irradiated extracts, and the $a^*\;and\;b^*$values decreased by the irradiation treatment. The content of the total phenolic compounds in the non-irradiated and irradiated extracts were 58.4 and 56.5 mg/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activities of non-irradiated and irradiated extracts at a 250 ppm level were 80% and 79%, respectively. Lipid oxidation was retarded by addition of Bokbunja powder. Nitrite scavenging activity was the highest in the Bokbunja powder at pH 1.2 and the effect was not changed by irradiation. The Bokbunja powder showed antimicrobial activity against Salmonella Typhimurium and Bacillus cereus. However, irradiation of Bokbunja did not affect any physiological functions (p>0.05). A Salmonella mutagenicity assay indicated that the irradiated Bokbunja extract did not show any mutagenicity. Therefore, Bokbunja extract could be used in various applications as a functional material, such as ingredients of food and cosmetic, compositions with functions.

Research on Methods of Developing Quadrangular Culm of Bamboos (사각죽조성(四角竹造成)에 관(關)한 연구(硏究))

  • Chong, Hyon-Pae
    • Journal of Korean Society of Forest Science
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    • v.10 no.1
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    • pp.7-16
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    • 1970
  • There are 19 species of bamboo in Korea, among which few are of commercial value. The Phyllostachys has wide range of utilization, for sea weed production, industrial production of various bamboo wares, etc. The total export from Chenla-Namdo to some more than ten countries was recorded 204,000,000 won worth in 1969. As civilization develops so does the techniques of bamboo works, and they in turn accelerate the demand for bamboo wood of better quality. In the light of this trend, the author attempted to make bamboo plants to develope a quadragular culm with elaborate design as disired instead of the original round one, with the expectation that it might be widely welcomed by hand workers, interior decorators. Here are some facts found out in this experiment: 1). The greater the diameter of the shoot at the eye level was, the better result was brought, and as shown in Fig. 5 the rate of success was 72% with the shoots of 7.5 cm thick. 2). The shoots of 30 cm in length showed 100% of success, developing into a desired quadrangular culms without fail(See Fig. 6). 3). The intensity of wood fiber increased as time lapsed, without receiving any influence by the weather (See Fig. 7, 8). 4). During the growing, the culm proved to be flexible enough to bend up to 90 degree (See Fig. 9). 5). In an attempt to promote the value of bamboo by decoration, the author tried to impress rectangular indents on the culm. The indention using a qudangular board 1cm smaller in width than the shoot diameter gave 100% success (See Fig. 10). 6). Design experiment was also successful both in coloration and impressing and the resulting designs delicately depended on the kinds of chemicals used and their combination (Table 2) (See Fig. 12, 13, 14). With the above mentioned findings, the author concludes that the mass production of quadrangular bamboo culm is quite a promissing as an new industry to develope bamboo works to a more valuable one. This research was carried out with the research fund provided by the Ministry of Education in 1969.

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