• Title/Summary/Keyword: 2중 파장법

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Determination of Pyribencarb and Its Metabolite KIE-9749 in Agricultural Commodities by High-Performance Liquid Chromatography (액체크로마토그래피를 이용한 농산물중 pyribencarb와 대사물 KIE-9749의 잔류 분석법)

  • Kwon, Hyeyoung;Hong, Su-Myeong;Kim, Taek-Kyum;Kim, Hyung-Jin;Moon, Byeong-Chul;Kyung, Kee-Sung
    • The Korean Journal of Pesticide Science
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    • v.20 no.3
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    • pp.236-246
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    • 2016
  • The aim of this study was to develop an analytical method for the determination of pyribencarb and its metabolite KIE-9749 in agricultural commodities. The experiment was performed with a range of concentrations $0.05{\sim}2.5{\mu}g/g$ in apple, green pepper, potato, hulled rice, soybean, pear, peach, grape and cucumber. Each samples were extracted with acetone and cleaned by dichloromethane/saline water partition and purified with Florisil solid phase extraction (SPE) cartridge and aminopropyl SPE cartridge. Pyribencarb and KIE-9749 were separated and quantified by HPLC/UVD at 265nm using acetonitrile and water as mobile phase. The recoveries of pyribencarb and KIE-9749 were within 78.3~108.4% and 73.9~113.7% with RSD below 12.2% and 15.0%, respectively. The limits of quantification (LOQ) were both $0.05{\mu}g/g$. LC/ESI-MS/MS was optimized for confirmation of residue identity.

Selective Separation and Determination of Iron with Ion-Exchange Resins (이온교환수지에 의한 철의 선택적 분리 및 정량)

  • Yong Soon Chung;Dong Won Kim;Seung Ho Kim;Dai Woon Lee
    • Journal of the Korean Chemical Society
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    • v.31 no.1
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    • pp.45-54
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    • 1987
  • Dowex 1-X8 resin ion exchanged with calcon carboxylic acid (CCA-Dowex 1-X8) and 2-methyl-8-hydroxyquinoline(MHQ) impregnated-Amberlite XAD-4 resin (MHQ-XAD-4) were examined for the separation and preconcentration of ferric ion from the various matrices. The stabilities of these resins were investigated, and their capacities on ferric ion were also measured. The effect of pH on the adsorption of ferric ion and matrix ions, such as Al(Ⅲ) and Ca(Ⅱ), was investigated to determine the optimun pH ranges. Separation and preconcentration of iron in aluminium foil and mineral water samples were studied by elution method with these resin columns. The recovered ions by 10ml of 2F nitric acid was determined by flame atomic spectrophotometry. SP-Sephadex C-25 column was used to separate ferrous and ferric ion in mineral water by stepwise elution with ferrozine and 1 % ascorbic acid-ferrozine solution. The concentrated and separated each ion could be determined spectrophotometrically at the analytical wavelenth of Fe(Ⅱ)-ferrozine complex (562nm).

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Fabrication and Properties of Au fine Particles Doped ZrO2 Thin Films by the Sol-gel Method (졸-겔법에 의한 Au 미립자 분산 ZrO2 박막의 제조와 특성)

  • 이승민;문종수
    • Journal of the Korean Ceramic Society
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    • v.40 no.5
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    • pp.475-480
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    • 2003
  • Nanocomposite of Au doped ZrO$_2$ films was prepared, which could be used as non-linear optic materials, selective absorption and transmission films. After heat treatment of prepared thin film by dip-coating method, the characteristics were investigated by X-ray diffraction, UV-VIS Spectrometer, Atomic Force Microscopy (AFM) and Scanning Electron Microscope (SEM). Film thickness was about 150 nm, the Au particle size was 15~35 nm. The thin film had a smooth surface roughness about 1.06 nm. Nonlinearity optics was found that films showed absorption peak at 600~650 nm visible region by plasma resonance of Au metal particles.

Determination of Metal Elements in Mongolian Chromite (몽골산 크롬철광 중의 금속성분 분석)

  • Choi, Kwang Soon;Lee, Chang Heon;Pyo, Hyung Yeol;Park, Soon Dal;Joe, Kih Soo
    • Analytical Science and Technology
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    • v.13 no.6
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    • pp.766-774
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    • 2000
  • The major and trace constituents of Mongolian chromite were analyzed by ICP-AES. The dissolution procedures, mixed acid ($HClO_4+H_3PO_4$) digestion and fusion with $Na_2O_2$ flux, have been studied to dissolve the chromite. The optimum dissolution method was found to be a fusion with $Na_2O_2$ flux. The effect of large amount of Na on major and trace constituents was examined when these elements were determined by ICP-AES. There was no effect on major elements at a concentration of Na 250 mg/L solution. The emission intensity of trace constituents containing Na 1,250 mg/L decreased to 1.0-5.2% according to elements and wavelengths. The result of this method was compared with that of neutron activation analysis (NAA) to confirm the accuracy of this procedure. The results between two methods were in a good agreement within less than 5% for $Al_2O_3$, $Cr_2O_3$, MgO and -20 to 8% for Co, Mn, V, Zn, respectively.

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Study on the Characterization of Light-induced Mitochondrial ATP Synthase in Pleurotus ostreatus (느타리버섯(Pleurotus ostreatus)중의 Light-induced mitochondrial ATP Synthase의 효소적 특성에 관한 연구)

  • Min, Tae-Jin;Lee, Kang-Hyeob
    • The Korean Journal of Mycology
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    • v.19 no.1
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    • pp.32-40
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    • 1991
  • Mitochondria in Pleurotus ostreatus were isolated and purified by the stepped sucrose density gradient centrifugation, to investigate the effects of the light on the enzymatic activity of the mitochondrial ATP synthase. This enzyme, which was illuminated by the light ranging from 400 nm to 700 nm, showed that the specific activity was stimulated at 490 nm for 15 sec. Effects of organic compounds on the mitochondrial ATP synthase were also investigated at the optimum conditions; The activities of this enzyme were increased to 168 percent by the addition of 2,6-dichlo­rophenol indophenol(DCPIP), 224 percent by phenazine methosulfate(PMS), but inhibited 91 per­cent by oligomycin, 14 percent by 2-heptyl-4-hydroxyquinoline-N-oxide(HQNO) and 75 percent by 2,4-dinitrophenol (DNP), respectively. Effects of metal ions of the mitochondrial ATP synthase were investigated at the optimum conditions. The activities of the enzyme were inhibited 35 percent by $Ca^{2+}$, 14 percent by $Co^{2+}$ and 73 percent by $Mn^{2+}$. For effects of anions, the activities of this enzyme were inhibited 80 percent by $CN^{-}$, 52 percent by $SO_{4}\;^{2-}$, 28 percent by each of $CO_{3}\;^{2-}$­and $NO_{3}\;^{-}$, respectively.

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유기 금속 화학 증착법에 의한 Si 기판 위에 GaP 층 성장시 에피의 초기 단계의 성장 매개 변수에 영향

  • Gang, Dae-Seon;Seo, Yeong-Seong;Kim, Seong-Min;Sin, Jae-Cheol;Han, Myeong-Su;Kim, Hyo-Jin
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.209.1-209.1
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    • 2013
  • GaP는 가시광선 발광다이오드을 얻을 수 있는 적절한 재료중의 하나로 해당영역의 파장에 대하여 높은 양자효율을 얻을 수 있고, 깊은 준위 재결합이 없기 때문에 GaP 녹색 및 As 첨가한 GaAsP 적색 LED 에 적용할 수 있습니다. 또한, 상온에서 2.2 eV 에 해당하는 넓은 에너지 밴드갭을 가지고 있으므로, 소음이 없는 자외선 검출기에도 적합합니다. 이 물질에 대한 소자들은 기존에 GaP 기판을 사용하였습니다. 최근, GaP 와 격자상수가 비슷한 Si 기판을 활용하여 그 위에 성장하는 방법에 대한 관심이 많아졌습니다. Si는 물리적 및 화학적으로 안정하고 딱딱한 소재이며 대면적 기판을 쉽게 얻을 수 있어 전자 기기 및 대규모 집적 회로의 좋은 소재입니다. Si 와 대조적으로 GaP은 깨지기 쉬운 재료이며 GaP 기판은 Si와 같은 대면적 기판을 얻을 수 없습니다. 이러한 문제의 한 가지 해결책은 Si 기판위에 GaP 층의 성장입니다. GaP 과 Si의 조합은 현재의 광전소자 들에 더하여 더 많은 응용프로그램들을 가능하게 할 것입니다. 그러나, Si 기판위에 GaP 성장 시 삼차원적 성장 및 역위상 경계면과 같은 문제점들이 발생하므로 질이 높고 균일한 결정의 GaP 를 얻기가 어렵습니다. 따라서, Si 에 GaP 의 성장시 초기 단계를 제어하는 성장 기술이 필요합니다. 본 연구에서는, 유기금속화학증착법을 이용하여 Si 기판위에 양질의 GaP를 얻을 수 있는 최적의 성장조건을 얻고자 합니다. 실험 조건은 Si에 GaP의 에피택셜 성장의 초기 단계에 영향을 주는 V/III 비율, 성장압력, 기판방향 등을 가변하는 조건으로 진행하였습니다. V/III 비율은 100~6400, 성장 압력은 76~380 Torr로 진행하였고, Si 기판은 just(001)과 2~6도 기울어진 (001) 기판을 사용하였습니다.

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Studies on the Development of Photoreceptor in the Nonchromatophore Organisms (III) -Light- Induced Mitochondrial ATP Synthase in the Lentinus edodes (Berk.) Sing.- (무흡광 색소 생물의 감광수용체 개발 연구(III) -표고버섯 중의 광감응성 Mitochondrial ATP Synthase-)

  • Min, Tae-Jin;Lee, Wan-Gie;Park, Sang-Shin
    • The Korean Journal of Mycology
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    • v.17 no.2
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    • pp.91-98
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    • 1989
  • Mitochondria in the L. edodes was separated and purified by stepped sucrose density gradient centrifugation. The activity of mitochondrial ATP synthase has been investigated during various illumination times at each wavelength within the range of 400 nm to 700 nm. The stimulation of above activity increased by two times compared with nonilluminated control group when the illumination was given for 15 seconds at 470 nm wavelength. The optimal pH and temperature of this light-induced mitochondrial ATP synthase were 7.5 and $54^{\circ}C$, respectively. The activity of this enzyme increased by 26%, 25% and 14%, respectively, when there were 1 mmole $Fe^{3+}$, 0.5 mmole $Fe^{2+}$, and 5 mmole ${SO_4}^{2-}$ ion, and was inhibited by 5 mmole $Co^{2+}$, 5 mmole $Mn^{2+}$, 1 mmole $Ca^{2+}$, 0.1 mmole $Na^+$, 5 mmole $CN^-$, and 0.1 mmole ${CO_3}^{2-}$ ion. But $Na^+$ and $K^+$ ion did not affect the activity of enzyme.

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Influence of Process Conditions on Properties of Cu2O Thin Films Grown by Electrodeposition (전착법을 이용한 Cu2O 박막 형성 및 공정 조건에 따른 특성 변화)

  • Cho, Jae Yu;Ha, Jun Seok;Ryu, Sang-Wan;Heo, Jaeyeong
    • Journal of the Microelectronics and Packaging Society
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    • v.24 no.2
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    • pp.37-41
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    • 2017
  • Cuprous oxide ($Cu_2O$) is one of the potential candidates as an absorber layer in ultra-low-cost solar cells. $Cu_2O$ is highly desirable semiconducting oxide material for use in solar energy conversion due to its direct band gap ($E_g={\sim}2.1eV$) and high absorption coefficient that absorbs visible light of wavelength up to 650 nm. In addition, $Cu_2O$ has other several advantages such as non-toxicity, low cost and also can be prepared with simple and cheap methods on large scale. In this work, we deposited the $Cu_2O$ thin films by electrodeposition on gold coated $SiO_2/Si$ wafers. We changed the process conditions such as pH of the solution, applied potential on working electrode, and solution temperature. Finally, we confirmed the structural properties of the thin films by XRD and SEM.

Study for Membrane Fouling Monitering Using Image Extraction by Visible Light Irradiation (가시광선 조사에 의한 이미지 추출법을 이용한 막 오염 모니터링 연구)

  • Park, Ah-Rum-I;Seo, Mi-Rae;Nam, Seung-Eun;Kim, Beom-Sik;Park, Ho-Bum;Kim, In-Chul;Park, You-In
    • Membrane Journal
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    • v.21 no.2
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    • pp.171-176
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    • 2011
  • Membrane fouling is formed due to pore blocking and cake formation by suspended material or contaminant in the membrane boundary layer. Membrane fouling is main obstacle for the wider application of industrial water treatment. The objective of this paper is to study the direct monitoring technique for the measuring the membrane fouling in real time. We investigated the extracted image of R, G, and B by visible light irradiation of 360 nm wavelength to measure the membrane fouling in real time by transparent foulant. The intensity of B of 400~499 nm wavelength range was stronger than that of R and G. The fluorescence image extraction analysis appeared to be a very attractive technique for monitoring the membrane fouling in real time.

Rapid Determination of Ginsenosides Rb1, Rf, and Rg1 in Korean Ginseng Using HPLC (HPLC를 이용한 고려인삼 중 진세노사이드 Rb1, Rf 및 Rg1의 신속분석 방법 개발)

  • Hong, Hee-Do;Choi, Sang-Yoon;Kim, Young-Chan;Lee, Young-Chul;Cho, Chang-Won
    • Journal of Ginseng Research
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    • v.33 no.1
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    • pp.8-12
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    • 2009
  • A simple gradient HPLC method for rapid determination of major ginsenosides ($Rg_1$ and $Rb_1$) and unique ginsenoside (Rf) of Korean ginseng (Panax ginseng C.A. Meyer) was developed. Within 50min, three ginsenosides have been separated and identified on $\mu$-Bondapak $C_{18}$ column ($3.9{\times}300\;mm$, $10{\mu}m$) with gradient elution using water and acetonitrile as a mobile phase. The method was validated in terms of linearity, accuracy, and precision. The correlation coefficients ($r^2$) for calibration curves of ginsenosides were over 0.9997. The developed HPLC method was successfully applied to the analysis of ginseng samples and the recoveries of ginsenosides were in the range of $101.1{\sim}115%$ with RSD<3.2%. The developed method could be used for rapid evaluation of the ginsenosides $Rg_1$, $Rb_1$, and Rf.