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http://dx.doi.org/10.7585/kjps.2016.20.3.236

Determination of Pyribencarb and Its Metabolite KIE-9749 in Agricultural Commodities by High-Performance Liquid Chromatography  

Kwon, Hyeyoung (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration)
Hong, Su-Myeong (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Taek-Kyum (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Hyung-Jin (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration)
Moon, Byeong-Chul (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kyung, Kee-Sung (Department of Environmental and Biological Chemistry, College of Agricultural Life and Environmental Sciences, Chungbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.20, no.3, 2016 , pp. 236-246 More about this Journal
Abstract
The aim of this study was to develop an analytical method for the determination of pyribencarb and its metabolite KIE-9749 in agricultural commodities. The experiment was performed with a range of concentrations $0.05{\sim}2.5{\mu}g/g$ in apple, green pepper, potato, hulled rice, soybean, pear, peach, grape and cucumber. Each samples were extracted with acetone and cleaned by dichloromethane/saline water partition and purified with Florisil solid phase extraction (SPE) cartridge and aminopropyl SPE cartridge. Pyribencarb and KIE-9749 were separated and quantified by HPLC/UVD at 265nm using acetonitrile and water as mobile phase. The recoveries of pyribencarb and KIE-9749 were within 78.3~108.4% and 73.9~113.7% with RSD below 12.2% and 15.0%, respectively. The limits of quantification (LOQ) were both $0.05{\mu}g/g$. LC/ESI-MS/MS was optimized for confirmation of residue identity.
Keywords
Analytical method; KIE-9749; pyribencarb; residue;
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