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Effect of Steroid Administration Ex Vivo on the IκB/NF-κB Pathway in Human Peripheral Blood Monocytes (스테로이드의 투여가 말초혈액 단핵구에서 IkB/NF-κB경로에 미치는 영향)

  • Yoon, Ho Il;Lee, Hee-Seok;Lee, Chang-Hoon;Lee, Choon-Taek;Kim, Young Whan;Han, Sung Koo;Shim, Young-Soo;Yoo, Chul-Gyu
    • Tuberculosis and Respiratory Diseases
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    • v.54 no.5
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    • pp.542-550
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    • 2003
  • Background : Synthetic glucocorticoids are widely used in many chronic inflammatory diseases because of their excellent anti-inflammatory activity. Enhancing the transcription of $I{\kappa}B$ and preventing activated NF-${\kappa}B$ from binding to ${\kappa}B$ sites are thought to be the underlying mechanisms. But these data are largely derived from in vitro studies using cell lines. In this study, after administrating a steroid to volunteers, we evaluated the effect on the NF-${\kappa}B$ system. Methods : Prednisolone(0.5mg/kg/d) was orally administered to 5 healthy volunteers for 7 days. Before and after the administration, we sampled their peripheral blood monocytes, and performed western blot analysis both with stimulation, using IL-$1{\beta}$, LPS, TNF, and without stimulation(baseline). We also performed EMSA after stimulation with LPS. Results : After ingestion of the steroid, baseline expressions of $I{\kappa}B{\alpha}$ were increased in two of the subjects, while suppressed degradations of $I{\kappa}B{\alpha}$ to stimulations were observed in all five. In addition, the binding capacity of NF-${\kappa}B$ after the administration was decreased. Conclusion : Steroid plays such roles as enhancing the transcription of $I{\kappa}B{\alpha}$, suppressing the DNA binding capacity of NF-${\kappa}B$, and suppressing the degradation of $I{\kappa}B{\alpha}$.

The Effects of Green Tea on the Serum Lipid and Liver Tissue of Cholesterol Fed Rats (녹차의 음용이 지방과 콜레스테롤을 투여한 흰쥐의 혈청지질 및 간의 조직에 미치는 영향)

  • Sin, Mee-Kung;Han, Sung-Hee;Han, Gun-Jo
    • Korean Journal of Food Science and Technology
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    • v.29 no.6
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    • pp.1255-1263
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    • 1997
  • This study was performed to investigate the effects of green tea on fat metabolism of rats and prevention to cardiovascular disease in drinking green tea. Male Spague-Dawley rats were divided into seven groups consisting of the control, lard and cholestrol, aqueous green tea at the level of 1% and 3%. After 4 weeks of feeding serum lipid levels were measured for experimental rats, and analyzed the total cholesterol (TC), HDL-cholesterol (HDL-C), triglyceride (TG), phospholipid (PL). And total lipid (TL) to Folch method, lipid deposition to oil red O staining on liver tissue. The results are as follows: Total cholesterol (TC) decreased by administration of 1% aqueous green tea group and increased addition to lard and cholesterol (LC) group as compared to each groups (p<0.05). HDL-cholestrol in serum increased by administration of la aqueous green tea group (1G) and decreased to the control group, 1% aqueous green tea (L-1G) added lard group (p<0.05). Triglyceride (TG) decreased by administration of 3% aqueous green tea groups (L-3G, LC-3G) and increased by lard and cholesterol group (LC) (p<0.01). Phospholipid(PL) decreased by administration of 3% aqueous green tea added lard and cholesterol group (LC-3G) and increased by control group, lard and cholestrol group (LC) (p<0.05). Total lipid of liver decreased by administration of aqueous green tea at the level of 1% group and increased by LC group (p<0.01). The fat deposition of liver was increased in fat diet groups and decrease in the drink green tea of some but did not showed significant differences from concentration of the green tea.

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Epidermal Changes of the Adhesive Disks During Wall Attachment in Parthenocissus tricuspidata (착생에 따른 담쟁이덩굴 흡착근 표피조직의 변화)

  • Kim, Jung-Ha;Kim, In-Sun
    • Applied Microscopy
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    • v.37 no.2
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    • pp.83-91
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    • 2007
  • The present study examined the epidermal changes of adhesive disks which occur during attachment in Parthenocissus tricuspidata using scanning and transmission electron microscopy. Several adhesive disks, each covered with a bract, develop from the shoot apical meristem during early development. In the initial stage, the adhesive disks are club-shaped and their upper and lower epidermis are indistinguishable. However, in the actively growing stage, they become spherical and both epidermis are clearly differentiated into the adventitious roots. Prior to wall attachment, the adhesive disks exhibit adaxial convex and abaxial concave shapes, and electron-dense substances are abundant in the vacuoles of epidermal cells. The peripheral area of the adhesive disk is adhered first to the wall surface, while the central area is drawn inward in a vacuum-like state during attachment. As the attachment progresses and the electron-dense substances continue to discharge, the upper and lower epidermis rapidly undergo deterioration and the disks shrink considerably. At this stage, structural changes of the lower epidermis occur much faster than in the upper one. The discharged substance is accumulated on the wall surface, and this aids the attachment of adhesive disks on the wall for long periods. In this manner, the shape and structure of the adhesive disk epidermis change drastically from initial growth to the mature stage. Further, the role of electron-dense substance and shrinkage of the disk during attachment has been discussed in Parthenocissus tricuspidata.

Expression of Growth Factors and Secretory Leukocyte Protease Inhibitor (SLPI) in RAW264.7 Cells after Lipopolysaccharide (LPS) Stimulation (대식세포주 (RAW264.7)에서 박테리아성 지질다당류 (LPS) 자극에 의한 분비백혈구단백분해효소억제제 (SLPI)와 성장인자들의 발현)

  • Son, Wook-Hee;Choi, Baik-Dong;Jeong, Soon-Jeong;Wang, Guan-Lin;Hwang, Ho-Keel;Jeong, Moon-Jin
    • Applied Microscopy
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    • v.37 no.2
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    • pp.93-102
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    • 2007
  • Secretory leukocyte protease inhibitor (SLPI) was known as one of bacterial lipopolysaccharide (LPS)-induced products of macrophage. Macrophages play an important role in the development of inflammatory responses by secreting an array of cytokines and chemokines in a tissue microenvironment. To identify the function and relationship between potent growth factors and SLPI after LPS stimulation, we conducted reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots for the detection of mRNA and protein expression of SLPI and growth factors such as VEGF, PDGF, bFGF after 100 ng LPS stimulation on the RAW264.7 cells. The result of RT-PCR was showed SLPI mRNA expression was increased from 60 min to 48h in RAW 264.7 cells after incubation with LPS. VEGF and PDGF mRNA was expressed highly at initial stage by LPS stimulation. The mRNA of bFGF and type I collagen was very weakly expressed after LPS stimulation. SLPI protein level was increased likely the mRNA levels in RAW 267.7 cells. Additionally, phase contrast and scanning electron microscopic observation demonstrated that the LPS induce the change of morphology of the RAW264.7 cells. From these results, it suggest that expression of SLPI by LPS treatment may associate with VEGF and PDGF expression in RAW264.7 cells.

A Study on Block Patterns for of Korean fashion Models (졸업작품 패션쇼 모델의 치수에 적합한 원형 연구)

  • Park, Sang-Hee;Kang, Kyoung-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.32 no.6
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    • pp.999-1011
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    • 2008
  • To most of the students studying fashion related major, the graduation fashion show is a big challenge. They have to put together all they learn and show what they can do to their future employers. They design, pattern work, and make up garments for the show all by themselves. Unfortunately. while they make up their garments, they usually don't Dow exactly body measurements of the models. So quite often they have to alter their art works up to the last minute of the fashion show opening. Sometimes such unadequate work process ruins their work. The purpose of this study is to suggest block patterns of Korean fashion models measurements for basic items, such as jacket and pants for male models and torso length block pattern, skirt and pants for female models. 20 male and 20 female professional models were measured. The block patterns were based on their measurements. After the first fitting test, patterns were corrected by their body characteristic. For both male and female models, it was found desirable to fix the shoulder width and make an adjustment to the patterns with a deviation of width and girth items. In case of the resultant patterns the satisfaction was made better. Model sizes proposed in this study are considered closer to the size of average models, since they were based on A-grade models who are currently working in Korea. The resultant patterns can be produced by simply making a slight adjustment to the width of the proposed pattern in this study.

Distribution of water Masses and Chemical Properties in the East Sea of korea in Spring 2005 (2005년 춘계 동해 중남부 해역의 수괴 분포 및 화학적 특성 연구)

  • Kim, Y.S.;Hwang, J.D.;Youn, S.H.;Yoon, S.C.;Hwang, U.G.;Shim, J.M.;Lee, Y.H.;Jin, H.G.
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.10 no.4
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    • pp.235-243
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    • 2007
  • In order to understand the water mass properties in the southern location of the East Sea in the Korean coasts, the vertical distributions (down to 2,000 m deep) of water temperature, salinity, and dissolved inorganic nutrients were determined in April of 2005. The water mass of the surveyed location showed distinct vertical layers; highly saline surface, surface mixing layer, and thermocline of low temperature and salinity. The water layer below 300 m was characterized by water temperature lower than $1^{\circ}C$ and salinity 34.06, showing a representative water mass of the East Sea. The inorganic nutrients rapidly increased from 200m in the northern and southern parts around Ulleung Basin. A marked environmental difference was found between two layers separated by thermocline. The upper layer of the thermocline was oligotrophic and the vertical distribution of nutrient was very stable. In the water layer between 100 and 200m the nutrients slightly increased but remained still stable. From southern coasts to northeastern Ulleung, the water mass properties were site specific; the thickness of the surface mixed layer and nutricline showed a trend diminishing toward the northern locations probably due to diminished influence of Tsushima water. Redfield ratio (N:P=16:1) based on the ratio of chemical composition in organism revealed that nitrogen value continuously decreased to less than 16 with the water depth down to loom from the thermocline. The value in the water layer deeper than 100 to 200 m, thereafter, showed an increasing trend (over 16). This result was further supported by the finding of lower chlrophyll a content in the layer.

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The Sr and Pb Isotopic and Geochemical Properties of the Atmospheric Bulk Deposition of Jeonju, Gunsan, and Namweon Areas (전주, 군산, 남원지역 강수의 Sr, Pb동위원소 지화학)

  • Jeon Seo-Ryeong;Chung Jae-il
    • Economic and Environmental Geology
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    • v.38 no.4 s.173
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    • pp.463-479
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    • 2005
  • The Sr and Pb isotopic ratios and chemical composition were measured for atmospheric bulk deposition samples collected in the Jeonju, Gunsan and Namweon areas over a period of one year. Acidity of deposition ranged pH $4\~7$ with little higher in dry season, and around pH 5.0 in rainy season. The EC and TDS of rainy season was low showing dilution effect, and increased during dry season. Sulfate $(SO_4)\;and\;NO_3$ are atmospheric aerosols largely of anthropogenic origin in winter. Sodium was concentrated in winter deposition, Ca was concentrated in spring to summer deposition. Namweon has lower EC and TDS than those of other, and Jeonju has higher. Namweon was concentrated in $HCO_3$ and Cunsan was concentrated in Cl. Aluminium, Cu, and Zn show good correlation index with TDS, indicating of their origin atmospheric. $^{87}Sr/^{86}Sr$ ratios of bulk deposition ranged from 0.7109 to 0.7128. The isotopic variations are correlated with mixing of isotopic compositions of local soils, road deposit and biogenic aerosol. In order to constrain further the origin of aerosols in rainwater, it will be necessary to collect additional Sr isotopic data for aerosols. Lead isotope ratios for all areas were similar and belonged to Pb isotope ratios of Seoul's aerosols, but little different with Beijing's aerosols. It showing that Pb in the Korea mainly derived from the gasoline combustion, not exclusively from the Beijing.

A Study for an Educational Direction in Fashion Design Subjected to Digital Environment I (디지털 환경에 따른 패션분야의 디자인 교육방향 연구 I)

  • 이미량
    • Archives of design research
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    • v.12 no.4
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    • pp.33-44
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    • 1999
  • Fashion is subjected to newness and change by itself. Fashion trend and tendency which could predict next season akeady indicated that digital and cybel11ation environment lliould be factors in fashion. Changes in future life styles and values caused by digital environment must be variables as fashion objects by themselves. Therefore digital environment of a fashion itself and that all encountered in fashion will not only be an existing situation but also an objective and a factor which must be pursued with fashionable sense in future fashion. Advanced technology and social change in relation to digital environment requires that educational method as well as its content will be changed on the scene of education for future. Thus we should seek an educational direction in fashion design for future society in which digital environment requires new paradigm. Subject to these backgrounds this study has an intension to study the change in fashion extensively influenced by expanded digital environment on a limited situational level of fashion design education and to suggest an educational direction for professional in future fashion design who will serve as a core of fashion area in the future. It is essential that education of professional in fashion for future should be achieved by digital-minded level that eagerly understands and accepts future society in which digital environment will be a main axis. It is necessary for this to make them expetience computers, digital media including CAD program. We have to note that education of digital media is more effective and applicable when accomplished in CAI level which can be related and expetienced with fashion education rather than mere functional training in media themselves. Intellectual creativity will be a vety source of producing values and national competence in 21st centtury. Education of design professional in fashion area must positively accept these environmental charactetistics in future society, as well as deeply reflect the education for the future.

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Reclassification of Winter Barley Cultivation Zones in Korea Based on Recent Evidences in Climate Change (최근의 기후변화를 고려한 가을보리 안전재배지대 구분)

  • Shim Kyo Moon;Lee Jeong Taek;Lee Yang Soo;Kim Gun Yeob
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.6 no.4
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    • pp.218-234
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    • 2004
  • Recent warm winters were evaluated for a possible shifting of the northern limit for winter barley cultivation in Korea. Therefore, safe zones for winter barley cultivation were reclassified based on the average and minimum January air temperature in recent years. The results are as follows: By analysis of mean values of January average air temperatures for 30 years (1971-2000), the northern limits for safe cultivation of hulled, naked, and malting barley were Ganghwa - Icheon - Chungju - Chunyang - Goseong, Cheonan - Geumsan - Mungyeong - Andong - Sokcho, and Gwangju - Jangheung - Sancheong - Pohang - Uljin lines, respectively. Meanwhile, based on the January average air temperature of 14 years (1987-2000) with warmer winters, the safe cultivation zone of winter barley shifted northward of the normal (1971-2000). So, the northern limits for hulled, naked, and malting barley were Pocheon - Chuncheon - Wonju - Yangpyeong - Chunyang, Ganghwa - Icheon - Chungju - Uiseong - Goseong, and Gunsan - Suncheon - Jinju - Miryang - Yeongdeok - Uljin lines, respectively. Winter barley cultivars with the strongest tolerance to low temperature can be grown up to the adjacent areas of Taebaek Mountains (that is, Inje, Hongcheon, Jecheon, and Taebaek areas). Based on January mean air temperatures of 10-year return period for 30 years (1971-2000), the northern limits for hulled and naked barley were Boryeong - Namwon - Geochang - Gumi - Goseong and Seocheon - Jeongeup - Hapcheon - Yeongdeok - Sokcho lines, respectively. It ~ppears that malting barley can be cultivated only at southern coastal areas (that is, Busan, Tongyeong, Yeosu, and Wando areas). On the other hand, based on the weather conditions of 14 years (1987-2000) with warmer winters, the northern limits for hulled, naked, and malting barley were Ganghwa - Icheon - Yeongju - Goseong, Seosan - Namwon - Mungyeong - Andong - Sokcho, and Gwangju - Jangheung - Sacheon - Ulsan - Uljin lines, respectively. The northern limit for winter barley cultivars including Olbori with the strongest tolerance to low temperature was the Ganghwa - Wonju - Chungju - Chunyang - Goseong line.

Report for Development of Korean Portable Cardiopulmonary Bypass Machine (한국형 이동식 심폐소생기 개발 보고 I. 실험견을 이용한 개흉식과 폐쇄식 심폐소생술 비교)

  • Kim, Hyoung-Mook;Lee, In-Sung;Baek, Man-Jong;Sun, Kyung;Kim, Kwang-Taik;Kim, Yeon-Soo;Kim, Maeng-Ho;Lee, Hye-Won;Lee, Kyu-Back;Kim, Hark-Jei
    • Journal of Chest Surgery
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    • v.31 no.9
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    • pp.827-836
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    • 1998
  • Background: Effective cardiopulmonary resuscitation (CPR) should provide acceptable hemodynamics for the vital organs during cardiac arrest and early restoration of spontaneous circulation that guarantees long-term, neurologically intact survival. CPR using heart-lung bypass has been suggested as an option for that use. This study was designed to determine the effectiveness of standard CPR techniques, closed-vs. open-chest CPR, which could be used in the future study verifying the role of heart-lung bypass CPR. Material and Method: By using adult mongrel dogs, closed-chest CPR (CCCPR, n=4) and open-chest CPR (OCCPR, n=5) were compared with respects to hemodynamics, restoration of spontaneous circulation(ROSC), and survival. Ventricular fibrillation-cardiac arrest (VF-CA) was induced by electrical shock in all animals. After 4 minutes of cardiac arrest, basic life support (BLS) was applied for 15 minutes and followed by advanced life support (ALS). ALS was maintained until achi ving ROSC but not longer than 30 minutes regardless of the recovery. Resuscitation procedures in either group were standardized by adopting the protocol of American Heart Association. Result: Prearrest baseline hemodynamic data was not different between two groups. During resuscitation, substantially higher systolic pressure was maintained in OCCPR group than in CCCPR group (45$\pm$15 vs. 33$\pm$11 mmHg during BLS, 83$\pm$36 vs. 44$\pm$15 mmHg during ALS; p=NS). Mean pulmonary arterial pressure went up to the level of mean systemic arterial pressures in CCCPR group and to half of that in OCCPR group, and had kept higher in CCCPR group throughout CPR (32$\pm$10 vs. 22$\pm$4 mmHg during BLS and 32$\pm$15 vs. 24$\pm$10 mmHg during ALS; p=NS). ROSC was obtained in 4 of 5 dogs receiving open-chest CPR and 2 of 4 closed-chest CPR. Prolonged survival was noted in all dogs in OCCPR group (6 to 1440 hours) but not in CCPR group (p<.05). Conclusion: These findings indicate that open-chest CPR can be more effective t maintain hemodynamics during cardiac arrest and to obtain restoration of spontaneous circulation and survival. Further experiment will be designed to compare heart-lung bypass CPR with open-chest CPR.

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