• Environmental Analysis Health and Toxicology
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• 제18권3호
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• pp.199-208
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• 2003

A new technique was proposed for the determination of alkylphenols, chlorophenols and bisphenol A in tap water samples. The sample preparation consists of a solid phase extraction (SPE) of alkylphenols, chlorophenols and bisphenol A from a water sample with XAD-4 and subsequent conversion to isobutyloxycarbonyl (isoBOC) derivatives or tert-butyldimethylsilyl (TBDMS) derivatives for sensitive analysis with the CC/MS SIM mode. The recoveries were 86.6 ∼ 105.2％ (isoBOC derivatization) and 97.6∼484.5％ (TBDMS derivatization), respectively. The limit of quantitation of alkylphenols, chlorophenols and bisphenol h for SIM were 0.001∼0.050 $\mu\textrm{g}$/1 (isoBOC derivatization) and 0.003∼0.050 $\mu\textrm{g}$/1 (TBDMS derivatization). The SIM responses were linear with the correlation coefficient varying 0.9755∼0.9981 (isoBOC derivatization), and 0.9908∼0.9996 (TBDMS derivatization). When these methods were applied to tap water samples, the range of concentrations were 22.8∼31.3 ng/1 in 2,4-dichlorophenol, 28.6∼70.3 ng/1 in pentachlorophenol, 15.2∼17.4 ng/1 in t-butylphenol, 10.8∼13.2 ng/1 in t-octylphenol and 17.6∼36.3 ng/l in bisphenol A, respectively.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.145-145
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• 2003

A method for the determination of alkylphenols, chlorophenols and bisphenol A in paper materials using GC/MS-SIM has been developed. Eleven endocrine disrupting chemicals (EDCs) of phenols in paper samples were extracted with acetonitrile. Also, solid-phase extraction (SPE) with XAD-4 and subsequent conversion to isobutoxycarbonyl derivatives or tert.-butyldimethylsilyl derivatives for sensitive analysis with the selected ion-monitoring (SIM) mode. The recoveries were 82.4∼108.8 % by area ratio of pheranthrene-d$\sub$10/ vs bisphenol A d$\sub$l6/. (isoBOC derivatization and TBDMS derivatization) The SIM responses were linear with the correlation coefficient varying 0.9717∼0.9995 (isoBOC derivatization), and 0.9842∼0.9980 (TBDMS derivatization). The range of concentrations was respectively, 0.95∼l.44 ng/g in 2,4-dichlorophenol, 1.01∼1.17 ng/g in t-butylphenol, 2.17∼5.84 ng/g in pentachlorophenol, 12.68∼14.88 ng/g in nonylphenol and 30.84∼153.72 ng/g in bisphenol A.

• 대한화학회지
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• 제50권6호
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• pp.454-463
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• 2006

폐수처리방법으로는 분해가 어려운 유해화합물을 효과적으로 정화처리 할 수 있는 방법 개발을 위해, 최근 주목받고 있는 새로운 AOP(advanced oxidation process, 고급산화법) 공정인 초음파분해와 전기분해를 함께 이용하여 연구하였다. 대상 물질은 TCE(trichloroethylene)와 2,4-DCP(2,4-dichlorophenol)이며, 각 공정의 분해효율과 특성을 구하기 위해 각 시료물질들에 대해 초기농도, 초기 pH, 초음파 power 그리고 전류밀도 등을 변화시키며 최적 분해 조건을 조사하였다. 또한 시료물질에 대한 라디칼 포착제로 알려진 NaHCO3의 영향을 조사하여 각 시료물질의 분해반응 경로를 간접적으로 확인한 결과는 다음과 같다. TCE는 열분해반응이 지배적이며, 2,4-DCP 경우는 열분해반응 못지않게 라디칼 분해반응도 경쟁적으로 일어난다는 것을 알 수 있었다. 초음파와 전기분해를 동시에 적용시켰을 때, 두 시료 모두에서 120분 이내, 65% 이상 제거율을 보였으며, 제거율은 초기농도가 높을 때 그리고 초음파 power와 전류밀도가 증가할수록 향상되었다. 그리고 초기수용액의 pH 영향 실험에서는 TCE는 산성수용액에서 제거율이 높지만 2,4-DCP의 경우에는 알칼리성 수용액에서 제거율이 높은 것으로 나타났다.

• 대한환경공학회지
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• 제32권9호
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• pp.900-904
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• 2010

본 연구에서는 다양한 재질의 가정용 고무호스들(PVC 재질 4종, PE 재질 1종 및 SC 재질 1종)에서의 유해화학물질 용출 특성을 조사하였다. 고무호스 재질별 페놀류 용출 실험결과, PVC 재질의 고무호스들 4종에서 체류시간 96시간 동안 0.36~1.97 mg/L의 페놀류가 용출되는 것으로 나타나 PVC 재질의 고스 호스들에서 가장 많이 용출되는 것으로 나타났다. PE 재질의 경우 PVC 재질에 비해 매우 낮은 용출농도를 나타내었으며 실리콘 재질에서는 용출이 일어나지 않았다. 또한, 체류시간별 총 페놀의 용출 농도변화는 체류시간에 비례하여 직선적으로 증가하는 경향을 나타내었다. 초기 잔류염소 농도에 따른 총 페놀의 용출 특성 비교에서는 초기 잔류염소가 0.5 mg/L로 존재할 경우가 잔류염소가 존재하지 않는 경우에 비해 3~3.2배 정도 높게 용출되었으며, 수온에 따른 총 페놀의 용출 특성 비교에서는 수온 $4^{\circ}C$에 비해 $25^{\circ}C$일 경우가 100~104배 정도 높은 용출 농도를 나타내었다. 페놀류 각각에 대한 용출농도를 조사한 결과에서 BPA의 용출농도가 가장 높은 것으로 나타났고, 다음으로 DEHP와 2-chlorophenol, 2,4,6-trichlorophenol, 2,4-dichlorophenol 순으로 나타났다.

• Journal of the Korean Wood Science and Technology
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• 제50권5호
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• pp.315-324
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• 2022

The termicidal activity and chemical components of wood vinegar from two sources of biomass, nipah fruit shells (NFS) and a mixture of shells and fiber (MSF), were evaluated against Coptotermes curvignathus. A no-choice test was carried out to evaluate their termicidal activity using filter paper samples treated with 2.5%, 5.0%, 7.5%, 10.0%, and 12.5% NFS or MSF vinegar. Both wood vinegars exhibited antitermitic activity against C. curvignathus. The results show that increased concentrations of NFS and MSF vinegar significantly increased termite mortality. In particular, the NFS vinegar caused complete mortality and the lowest filter paper mass losses at 2.18% when treated with 12.5% wood vinegar. The most abundant chemical compounds of NFS vinegar were cyclopropanecarbonyl chloride, 2,5-dichlorophenol, 2-propanone, acetic acid, propanoic acid, benzenesulfonic acid, 3,7-dimethyl-6-octenal, and trans-geraniol. Meanwhile, the main compounds in the MSF vinegar were 1,2-ethanediol, formic acid, acetic acid, ethanoic acid, 2-furancarboxaldehyde, phenol, 2-methoxy phenol, and 4-methyl phenol.

• Journal of Plant Biology
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• 제36권3호
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• pp.259-266
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• 1993

In Tris-iso-butanol (TIB; Tris buffer pH 8.8 and 1% iso-butanol)-treated chloroplasts, oxygen evolving activity was more inhibited than Tris-treated chloroplasts, but restored highly by 2,6-dichlorophenol-indophenol (DCPIP) and photoreactivation. To understand the mechanism of this results of TIB in photosynthetic electron transport, system, oxygen consumption and evolution of PS I and PS II were measured and protein of the chloroplasts was analysed. In Tris- and TIB-treated chloroplasts, oxygen evolving activity was increased according to the light intensity. Under 48 W·m-2 light intensity, the oxygen evolving activity in both chloroplasts were similar but as the light intensity was increased, TIB-treated chloroplasts showed higher activity. Under 240 W·m-2 light intensity, TIB-treated chloroplasts showed about 25% higher oxygen evolving activity than Tris-treated chloroplasts. Oxygen evolving activity was increased after photoreactivation in both Tris-treated and TIB-treated chloroplasts. Addition of NH4Cl increased the activity in both chloroplasts but in TIB-treated chloroplasts the increase was 30% higher than that in Tris-treated chloroplasts. In PS I, oxygen evolving activity was not inhibited by both treatments whereas in PS II, significant difference was observed between two treatments. Addition of Mn2+ and Ca2+ enhanced oxygen evolution in both Tris- and TIB-treated chloroplasts. Though enhancement was higher in TIB-treated chloroplasts. No difference was observed n protein analysis of the two thylakoid membrane.

• 대한수의학회지
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• 제11권1호
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• pp.49-53
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• 1971

The anthelmentic effects of Fuadin (sodium antimony-III-bis-pyrocatechin-disulphonate), Bithionol 2-2-thiobis 4,6-dichlorophenol)), hexachlorethane and carbon tetrachloride were studied on Eurytrema pancreaticum infected in sheep and goats. The evaluation on the effects was based upon the egg reduction in the feces of the experimental animals administered the drugs. The number of eggs per 5gm. of the feces was respectively calculated before the treatments, and the follw-up for the egg reductions was carried out over a period of three or four weeks(calculated twice a week). Oral administration of Bithionol (once a day for 2 succeed days, at the rate of 75 mg per kg of bady weight) and of hexachlorethane (once a day, at the rate of 300ml per kg of body weight) did not reveal any egg reduction in sheep host. Intramuscular injection of carbon tetrachloride with olive oil (once a day, at the rate of 0.05ml per kg of body weight) did not show the egg reduction in goats. Intramuscular injections of Fuadin for 5 days (the dosage schedule was 0.5ml at 1st day, 1.0ml at 2nd and 3rd day, 1.5ml at 4th and 5th day, to amount of 5.5ml) were completely devoid the fluke eggs in the feces of goats on 4 weeks after each treatment. Throughout the studies, Fuadin was only proved its anthelmintic effect to the goats infected with E. pancreaticum. On the other hand, Bithional, hexachlorethane and carbon tetrachloride did not reveal any effect as Fuadin showed.

• 대한임상검사과학회지
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• 제38권2호
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• pp.87-93
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• 2006

This study was done to know a kind and change (transition) of enzymes produceed by Streptomyces halstedii ssp. scabies SA1-27 and Streptomyces violaceusinger C1-6 which showed good lignolytic activity and a good decolorization ratio of remazol brilliant blue R(RBBR) dye. These strains were isolated from soil and identified by the author. The basal medium containg 0.2% glucose was used to measure enzyme activity, Lignin peroxidase 1 (Lip 1) was measured by the methods of Choi, and Bourbonnais and Paice. Lignin peroxidase 2 (Lip 2) was measured by the methods of Ishida et al and Ramachandra et al using 2.4-dichlorophenol(2.4 DCP), manganese peroxidase(Mnp), veratryl alcohol oxidase (VAO), and laccase. They were measured by each of the methods of Choi and Paszczynski et al, and Bourbonnais and Paice, and De Jong et al. In the results, the kind of enzymes produced by Streptomyces halstedii ssp. scabies SA1-27 were Lip 1, Lip 2, VAO, and laccase, and their activities indicated the highest value as each 4.95 nmol/mg protein, $8.45({\times}100^{-3})unit$, 10.25 nmol/mg protein, 9.20 nmol/mg protein on the sixth day of the culture and decreased gradually over time. The kind of enzymes produced by Streptomyces violaceusinger C1-6 were Lip 1, Lip 2, Mnp, VAO, and laccase, and their activities indicated the highest value as each 4.90 nmol/mg protein, $13.85({\times}100^{-3})unit$, 3.10 nmol/mg protein, 11.30 nmol/mg protein, 4.45 nmol/mg protein on the sixth day of the culture and decreased gradually over time. Consequently, the author knew the fact that there were few differences in the kind and quantity of enzymes produced by the two Streptomyces strains, but all enzyme activities indicated the highest value on the sixth day of the culture and decreased gradually over time.

• Environmental Analysis Health and Toxicology
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• 제18권1호
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• pp.45-55
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• 2003

The alkylphenols, chlorophenols and bisphenol A were determined by gas chromatography/mass spectrom-etry-selected ion monitoring (GC/MS-SIM) mode followed by two work-up methods for comparison; isoBOC derivatization method and TBDMS derivatization method. Eleven phenols in paper samples were extracted with acetonitrile. Also, solid -phase extraction (SPE) with XAD -4 and subsequent conversion to isobutoxycarbonyl derivatives or tert.-butyldimethylsilyl derivatives for sensitive analysis with the selected ion-monitoring (SIM) mode. The SIM responses were linear with the correlation coefficient varying 0.9717 ∼ 0.9995 (isoBOC derivatization), and 0.9842 ∼ 0.9980 (TBDMS derivatization). The recoveries were 82.4 ∼ 108.8％) by area ratio of phenanthrene -d$\_$10/ vs bisphenol A-d$\^$l6/. (isoBOC derivatization and TBDMS derivatization) The range of concentrations was respectively, 0.95 ∼ 1.44 ng/g in 2,4-dichlorophenol, 1.01 ∼ 1.17 ng/g in t-butylphenol,2.17 ∼ 5.84 ng/g in pentachlorophenol, 12.68 ∼ 14.88 ng/g in nonylphenol and 30.84 ∼ 153.72 ng/g in bisphenol A.

• 대한가정학회지
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• 제4권1호
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• pp.589-594
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• 1963

Ascorbic acid has a strong power to reduce other materials, so by using this reducing power we can analise many materials quantitatively. Dihydro-ascorbic acid is quantitatively changed into ascorbic acid under a certain condition, if reduced by hydrogen-sulfide. Dihydro-ascorbic acid also has physiolosical effect, but less effect than ascorbic acid. The effect of dihydro-ascorbic acid is regarded as a half of that of ascorbic acid. Among the analytical methods applying this theory the method using 2.6 dichlorophenol indophenol what called the method of Indophenol is used most widely; so does Hydrazine-method. But comparing these two methods the former shows a little higher rate in analytical value. Vegetable are vital sources for vitamin C. According to the report of the commitee of FAO Korean branch the amount of daily per head average intake of vitamin C is about 70mg in raw materials. Since vitamin C is easily affected by heating or oxidation in Cooking the loss is not a little. Consequntly it is regarded that the actual amount of intake will be much less than basic amount. It is therefore very important to find out that how much percent of the loss there will be in case of cooking, in order to dicide the actual amount of in take as proper nutrition for a person. Therefore this paper intended to give some help in setting a standard amount of V.C intake, by measuring the change of the V.C amount using the general cooking method and by measuring amount of V.C contained in the part of vegetable, used in Korea abundantly.