Purpose: This study aimed to identify the mental fitness, perspective taking, attitude toward the elderly, and quantitative encephalogram among caregivers. Methods: In this mixed method research, 14 female caregivers at an elderly care facility in Y city were participated from December 1st to December 31st, 2019. Structured questionnaires were applied to measure general characteristics, mental fitness, perspective taking, and attitude toward the elderly. Brain waves were measured by quantitative encephalogram in the prefrontal lobe. High frequency and high level of care tasks were analyzed using content analysis. Results: According to analysis of brain waves, caregivers experienced high level of physical strain & stress and psychological distraction & stress. Mental fitness (p<.05) and perspective taking (p<.001) were associated with attitude toward the elderly. Moreover, physical strain & stress was associated with psychological distraction & stress (p<.05, p<.001). High frequency and high level tasks were excretion and position change & movement care related tasks, respectively. Conclusion: These findings should be consider as an evidence for supporting care tasks based on concrete and objective understanding of caregivers using self-reported index as well as neurophysiological indicators including brain waves.
This study investigated the effects of cantilever length, location and load condition on stress distribution developed in the implants, prostheses and supporting tissues. The osseointegrated prostheses with two 10mm Branemark implants at 2nd premolar and 1st molar sites with cantilever extensions at 1st premolar, 2nd and 3rd molar sites were constructed. Under 100N, 200N of vertical and $45^{\circ}$ oblique loads at the cantilever pontics, stress distribution patterns and displacement were analyzed with three dimensional finite element method. The results were as follows : 1. The stress was concentrated at the joint of the cantilever pontic and implant superstructure, the neck of implant and the ridge crest near the cantilever But there was little load transfer to the lower supporting tissues of implants. 2. The implant near the cantilever was displaced inferiorly while the implant far from the cantilever was displaced superiorly. In horizontal direction the implants were displaced to the direction where the loads were applied, except the apexes of the implants. 3. In case of anterior cantilever, the stress and displacement were higher than the prosthesis connected with natural tooth. 4. The stress developed in the posterior cantilevered type was higher than in the anterior cantilevered type. The greastest stress was concentrated at the ridge crest near the posterior cantilever. 5. The longer the cantilever, the more the stress was developed and was concentrated at the joint of the cantilever pontic and implant superstructure. 6. Under oblique load, the stress was concentrated at the necks of implants and the ridge crests, but decreased at the joint of the cantilever pontic and implant superstructure than under vertical load.
Background: We evaluated the effect of particulate matter (PM) and cigarette smoke extract (CSE) on bronchial epithelial cell survival, as well as oxidative stress and autophagy levels. Moreover, we aimed to assess the effect of the antioxidant N-acetylcysteine (NAC) on the adverse effects of PM and CSE exposure. Methods: Normal human bronchial epithelial cells (BEAS-2B cells) were exposed to urban PM with or without CSE, after which cytotoxic effects, including oxidative stress and autophagy levels, were measured. After identifying the toxic effects of urban PM and CSE exposure, the effects of NAC treatment on cell damage were evaluated. Results: Urban PM significantly decreased cell viability in a concentration-dependent manner, which was further aggravated by simultaneous treatment with CSE. Notably, pretreatment with NAC at 10 mM for 1 hour reversed the cytotoxic effects of PM and CSE co-exposure. Treatment with 1, 5, and 10 mM NAC was shown to decrease reactive oxygen species levels induced by exposure to both PM and CSE. Additionally, the autophagy response assessed via LC3B expression was increased by PM and CSE exposure, and this also attenuated by NAC treatment. Conclusion: The toxic effects of PM and CSE co-exposure on human bronchial epithelial cells, including decreased cell viability and increased oxidative stress and autophagy levels, could be partly prevented by NAC treatment.
Jun Sik Woo;Gyeong Rim Ryu;Jeong Hoon Kim;Sun Sik Jang;Hong Gu Lee;Keun Kyu Park
Animal Bioscience
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제37권7호
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pp.1303-1315
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2024
Objective: This study aimed to determine the effects of increasing energy and protein levels in diets by including protected fat (PF), glycerol (GL), and soybean meal (SBM) on growth performance, physiological parameters, carcass characteristics, and behavioral measurements of late-fattening Hanwoo steers under heat stress conditions. Methods: Thirty-six steers (initial body weight, 724.9±58.3 kg; age, 25.5±0.4 month) were assigned into control (total digestible nutrient [TDN] 76%, crude protein [CP] 15%), PF (TDN 83.6%, CP 15%), PF+GL (TDN 83.6%, CP 15%) and PF+GL+SBM (TDN 83.6%, CP 16.5%) by randomized complete block design for a total of 16 weeks with division of 4-week periods. The average temperature-humidity index was 87.0 (1st period; severe), 82.8 (2nd; moderate), 71.4 (3rd; comfort), and 68.1 (4th; comfort). Results: The dry matter intake (DMI) showed no treatments differences during the whole experiment. However, DMI in 1st and 2nd period decreased by approximately 30% and 10% compared to 4th period, respectively. Higher average daily gain and feed conversion ratio were noted for treatments compared to control at both 1st and 2nd period (p<0.05). There were no treatment effects on rectal temperature (RT), cortisol, and behaviors during the entire experiment. However, both RT and cortisol in 0, 1st and 2nd period were higher than those of 3rd and 4th period (p<0.05). Carcass yield and grade remained unaffected by increasing TDN and CP levels. Behavioral changes in the hot season (1st period) included reduced lying (43%), increased standing (48%), decreased walking (62%), and decreased eating (38%) (p<0.05), with an increase in drinking by 54%. Rumination during standing was 53% higher, while rumination during lying was about 33% lower compared to the post-hot season (3rd period) (p<0.05). Conclusion: Dietary supplementation of protected fat in late-fattening Hanwoo steers under heat stress had a positive effect on preventing a reduction in performance.
Purpose: This paper presents the effects of soil drought stress during the growing season and pre-harvest period on tree growth and fruit quality of "Yumi" peach, an early season cultivar. Methods: Soil drought stresses were treated with four levels of -30, -50, -60, and -70 kPa during long term (LT) and short term (ST). For LT treatments, soil water was controlled for nine weeks from May 1 to July 5, which was assumed as the full growing season. For ST treatments, soil water was controlled for four weeks from June 10 to July 5, which was assumed as the pre-harvest season. Tree growth and leaf photosynthesis were measured, and fruit characteristics such as fruit weight and diameter, soluble solid and tannin contents, and harvest date were investigated. Results: Soil water deficit treatments caused a significant reduction in tree growth, leaf photosynthesis, and fruit enlargement. LT water stress over -60 kPa during the full growing season caused significant reduction in tree growth, including shoot length, trunk girth, leaf photosynthesis, and fruit enlargement. ST water stress over -60 kPa during the pre-harvest period also induced significant reduction in leaf photosynthesis and fruit enlargement, while tree growth was not reduced. In terms of fruit quality, water stress over -50 kPa significantly reduced fruit weight, increased soluble solid and tannin contents, and delayed harvest time in both LT and ST treatments. Conclusions: As a result, it is assumed that LT water stress over -60 kPa can reduce both tree growth and fruit enlargement, whereas ST water stress over -50 kPa can reduce fruit enlargement without reducing tree growth. From an agricultural perspective, moderate water deficit like -50 kPa treatments could have positive effects, such increased fruit soluble solid contents along with minimal reduction in fruit size.
Purpose: This study was aimed to investigate the effects of Lebed method exercise (LME) on nurses' depression, anxiety and stress. The LME is a therapeutic exercise and movement program developed by Sherry Lebed Davis and expected to lessen the stress level of nurses and enhance the nursing job's satisfaction and efficiency. This study was utilized a non equivalent control group pre-post test design. Method: The subjects were 36 nurses in total; 18 in experimental group and 18 in control group. The data were collected from March to August, 2008. For the experimental group, 8 hour-long lectures on stress management and LME were given for 12 weeks. For the control group, only lectures on stress management was given. Depression, anxiety, perceived stress, and heart rate variability were measured on the subjects in both groups as pre- and post tests. The data were analyzed by Kolmogrov-Smirov test and P-P plot, t-test and $x^2-test$ using the SPSS program. Results: As proposed in the hypothesis, the subjects in the experimental group experienced less depression (t=2.286, p=.029), less anxiety (t=3.319, p=.002) and less perceived stress(t=2.862, p=.007) than those in the control group. Conclusion: The LME is considered an effective exercise to improve depression, anxiety, and to lessen stress for the nurses. The LME program has potential to be one of the effective stress management interventions for nurses in the future.
Purpose: This study aimed to identify the influence of job stress, compassion satisfaction, and resilience on depression of nurses. Methods: One hundred seventy six nurses working at one tertiary hospital in one metropolitan city were included. The data collection was conducted from September 1st to October 1st, 2018, using a structured, self-reported questionnaire. The collected data were analyzed using descriptive statistics, t-test, one-way ANOVA, Pearson's correlation coefficients, and multiple regression. Results: The $M{\pm}SD$ age of 170 subjects was $29.62{\pm}5.59$. Depression had significant negative correlations with compassion satisfaction (r=-.50, p<.001) and resilience (r=-.56, p<.001), and a positive correlation with job stress (r=.42, p<.001). In the multiple regression analysis, marital status (${\beta}=-.15$, p=.015), job stress (${\beta}=-.00$, p<.001) and resilience (${\beta}=-5.47$, p<.001) had a significant effect on depression. The explanatory power of the subject's marital status, leisure life, job stress, compassion satisfaction, and resilience on depression was 44.1% (F=27.62, p<.001). Conclusion: The study results showed that single status, low job stress, high resilience may decrease depression of nurses. Reducing job stress and improving resilience of nurses will virtually contribute to reduce their depression that can influence on not only nurses' health status but also their performance and qualitative caring for patients.
Purpose: We investigated the effect of far-infrared radiating products on cold hypersensitivity of lower limbs using CST. Methods: 7 patients with cold hypersensitivity of lower limbs were investigated in this study. Exclusive criteria was skin diseases, spinal nervous disease and external wounds. They were asked to answer the VAS of cold hypersensitivity at baseline and after 3 weeks. We measured temperature of lower limbs with Spectrum 9000 MB (Dorex Inc, USA). We performed cold stress test (CST) by 3 thermographic observation using DITI : 1st was taken after 15 minutes resting at $25^{\circ}C$, the 2nd was immediately taken after 1 minute soak in $20^{\circ}C$ water, the 3rd was taken at 15 minutes after soak. We performed 3 times of CST : 1st CST was perfomed at baseline, 2nd CST was perfomred after 1 week and just observation, 3rd CST was performed after 1 week using far-infrared radiating products (lasner, UMT, Korea). Results: After using products, temperature of foot incresed more than thigh area, but there was no significance. There was no statistical difference of VAS, change of temperature and CST between before and after using far-infrared radiating products. Conclusion: There was no statistical effect of far-infrared radiating products on change of temperature of lower limbs.
Tan, Shaun S.;Han, Xiaolian;Sivakumaran, Priyadharshini;Lim, Shiang Y.;Morrison, Wayne A.
Archives of Plastic Surgery
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제43권3호
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pp.237-241
/
2016
Background Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their subsequent viability. By functioning as an antioxidant against free radicals, melatonin may exert cytoprotective effects on ASCs. Methods We cultured human ASCs in the presence of varying dosages of hydrogen peroxide and/or melatonin for a period of 3 hours. Cell viability and apoptosis were determined with propidium iodide and Hoechst 33342 staining under fluorescence microscopy. Results Hydrogen peroxide (1-2.5 mM) treatment resulted in an incremental increase in cell death. 2 mM hydrogen peroxide was thereafter selected as the dose for co-treatment with melatonin. Melatonin alone had no adverse effects on ASCs. Co-treatment of ASCs with melatonin in the presence of hydrogen peroxide protected ASCs from cell death in a dose-dependent manner, and afforded maximal protection at $100{\mu}M$ (n=4, one-way analysis of variance P<0.001). Melatonin co-treated ASCs displayed significantly fewer apoptotic cells, as demonstrated by condensed and fragmented nuclei under fluorescence microscopy. Conclusions Melatonin possesses cytoprotective properties against oxidative stress in human ASCs and might be a useful adjunct in fat grafting and cell-assisted lipotransfer.
We performed 1st day Tc-99m-sestamibi gated SPECT with dipyridamole/rest T1-201 SPECT and 2nd day 24 hour delay T1-201 SPECT/rest Tc-99m-sestamibi gated SPECT in 27 patients with coronary artery disease(24) or having chest pain(3). Stress and rest Tc-99m-sestamibi gated SPECT was acquired at 60min post-injection. A 4-point scoring system(0 to 3 for normal to absent tracer uptake) for 17 segments was used. Wall motion was scored on another 4 point scale(0 to 3 for normal to dyskinesia) in the 1st day post-stress gated and the 2nd day rest gated SPECT. Post-stress gated SPECT showed wall motion abnormality in 94 segments(20%). Fifty-five segments among these 94 showed the same wall motion between post-stress and rest gated SPECT: i.e. 1-1 23 segments, 2-2: 29 segments, 3-3: 3 segments. Remaining 39 segments(41.5%) showed different wall motion between post- stress and rest Tc-99m-sestamibi gated SPECT. Twenty one segments with wall motion abnormality had normal perfusion(rest : 15 segments, 24 hour delay: 6 segments) at either rest or 24 hour delay. Fifteen among these 21 segments showed persistent post-stress and the 2nd day rest wall motion abnormality(persistent stunning). However, in 6 segments with pro-longed (1 hour after stress) stunning, abnormal wall motion did improve in the 2nd day rest Tc-99m-sestamibi gated SPECT(transient prolonged stunning). These 6 segments had normal perfusion at rest(n=4) or at 24 hour delay(n=2). Post stress wall motions showed significantly higher scores in persistent stunning than in prolonged transient stunning(P value<0.05). It was concluded that we could find stunned myocardium with gated Tc-99m-sestamibi SPECT at either post-stress or rest and that some myocardial walls of post-stress 1 hour gated SPECT did not show truly rest wall motion. So, we should be cautious if we use post-stress Tc-99m-sestamibi wall motion to assess rest wall motion.
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