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Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death

  • Tan, Shaun S. (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) ;
  • Han, Xiaolian (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) ;
  • Sivakumaran, Priyadharshini (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) ;
  • Lim, Shiang Y. (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) ;
  • Morrison, Wayne A. (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research)
  • Received : 2015.12.24
  • Accepted : 2016.04.12
  • Published : 2016.05.18

Abstract

Background Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their subsequent viability. By functioning as an antioxidant against free radicals, melatonin may exert cytoprotective effects on ASCs. Methods We cultured human ASCs in the presence of varying dosages of hydrogen peroxide and/or melatonin for a period of 3 hours. Cell viability and apoptosis were determined with propidium iodide and Hoechst 33342 staining under fluorescence microscopy. Results Hydrogen peroxide (1-2.5 mM) treatment resulted in an incremental increase in cell death. 2 mM hydrogen peroxide was thereafter selected as the dose for co-treatment with melatonin. Melatonin alone had no adverse effects on ASCs. Co-treatment of ASCs with melatonin in the presence of hydrogen peroxide protected ASCs from cell death in a dose-dependent manner, and afforded maximal protection at $100{\mu}M$ (n=4, one-way analysis of variance P<0.001). Melatonin co-treated ASCs displayed significantly fewer apoptotic cells, as demonstrated by condensed and fragmented nuclei under fluorescence microscopy. Conclusions Melatonin possesses cytoprotective properties against oxidative stress in human ASCs and might be a useful adjunct in fat grafting and cell-assisted lipotransfer.

Keywords

References

  1. Fraser JK, Wulur I, Alfonso Z, et al. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 2006;24:150-4. https://doi.org/10.1016/j.tibtech.2006.01.010
  2. Li CY, Wu XY, Tong JB, et al. Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy. Stem Cell Res Ther 2015;6:55. https://doi.org/10.1186/s13287-015-0066-5
  3. Monsel A, Zhu YG, Gennai S, et al. Cell-based therapy for acute organ injury: preclinical evidence and ongoing clinical trials using mesenchymal stem cells. Anesthesiology 2014; 121:1099-121. https://doi.org/10.1097/ALN.0000000000000446
  4. Luchetti F, Canonico B, Betti M, et al. Melatonin signaling and cell protection function. FASEB J 2010;24:3603-24. https://doi.org/10.1096/fj.10-154450
  5. Sanchez-Barcelo EJ, Mediavilla MD, Tan DX, et al. Clinical uses of melatonin: evaluation of human trials. Curr Med Chem 2010;17:2070-95. https://doi.org/10.2174/092986710791233689
  6. Deavall DG, Martin EA, Horner JM, et al. Drug-induced oxidative stress and toxicity. J Toxicol 2012;2012:645460.
  7. Yeh CH, Ma KH, Liu PS, et al. Baicalein Decreases Hydrogen Peroxide-Induced Damage to NG108-15 Cells via Upregulation of Nrf2. J Cell Physiol 2015;230:1840-51. https://doi.org/10.1002/jcp.24900
  8. Whittemore ER, Loo DT, Watt JA, et al. A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture. Neuroscience 1995;67:921-32. https://doi.org/10.1016/0306-4522(95)00108-U
  9. Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Meth 2012;9:671-5. https://doi.org/10.1038/nmeth.2089
  10. Mashiko T, Yoshimura K. How does fat survive and remodel after grafting? Clin Plast Surg 2015;42:181-90. https://doi.org/10.1016/j.cps.2014.12.008
  11. Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg 2008;34:1178-85.
  12. Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg 2008;32:48-55. https://doi.org/10.1007/s00266-007-9019-4
  13. Hsiao ST, Dilley RJ, Dusting GJ, et al. Ischemic preconditioning for cell-based therapy and tissue engineering. Pharmacol Ther 2014;142:141-53. https://doi.org/10.1016/j.pharmthera.2013.12.002
  14. Lim HD, Kim YS, Ko SH, et al. Cytoprotective and anti-inflammatory effects of melatonin in hydrogen peroxide-stimulated CHON-001 human chondrocyte cell line and rabbit model of osteoarthritis via the SIRT1 pathway. J Pineal Res 2012;53:225-37. https://doi.org/10.1111/j.1600-079X.2012.00991.x
  15. Kim SH, Lee SM. Cytoprotective effects of melatonin against necrosis and apoptosis induced by ischemia/reperfusion injury in rat liver. J Pineal Res 2008;44:165-71. https://doi.org/10.1111/j.1600-079X.2007.00504.x
  16. Chen HH, Chang CL, Lin KC, et al. Melatonin augments apoptotic adipose-derived mesenchymal stem cell treatment against sepsis-induced acute lung injury. Am J Transl Res 2014;6:439-58.
  17. Zhu P, Liu J, Shi J, et al. Melatonin protects ADSCs from ROS and enhances their therapeutic potency in a rat model of myocardial infarction. J Cell Mol Med 2015;19:2232-43. https://doi.org/10.1111/jcmm.12610
  18. Amiri F, Jahanian-Najafabadi A, Roudkenar MH. In vitro augmentation of mesenchymal stem cells viability in stressful microenvironments: in vitro augmentation of mesenchymal stem cells viability. Cell Stress Chaperones 2015;20: 237-51. https://doi.org/10.1007/s12192-014-0560-1
  19. Reynolds AR. Potential relevance of bell-shaped and u-shaped dose-responses for the therapeutic targeting of angiogenesis in cancer. Dose Response 2009;8:253-84.
  20. Owen SC, Doak AK, Ganesh AN, et al. Colloidal drug formulations can explain "bell-shaped" concentration-response curves. ACS Chem Biol 2014;9:777-84. https://doi.org/10.1021/cb4007584

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