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Selective Monitoring of Rutin and Quercetin based on a Novel Multi-wall Carbon Nanotube-coated Glassy Carbon Electrode Modified with Microbial Carbohydrates α-Cyclosophorohexadecaose and Succinoglycan Monomer M3

  • Jin, Joon-Hyung;Cho, Eun-Ae;Kwon, Chan-Ho;Jung, Seun-Ho
    • Bulletin of the Korean Chemical Society
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    • v.31 no.7
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    • pp.1897-1901
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    • 2010
  • Multi-wall carbon nanotube (MWNT)-modified glassy carbon electrodes (GCE) were prepared for simultaneous determination of rutin and quercetin. Microbial carbohydrates, $\alpha$-cyclosophorohexadecaose ($\alpha$-C16) and succinoglycan monomer M3 (M3) were doped into MWNTs to prepare a $\alpha$-C16-doped MWNT-modified GCE (($\alpha$-C16 + MWNTs)/GCE) and a M3-doped MWNT-modified GCE ((M3 + MWNTs)/GCE), respectively. The sensitivities of the ($\alpha$-C16 + MWNTs)/GCE to rutin and quercetin were 34.7 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$ and 18.3 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$, respectively, in a linear range of $2\sim8{\mu}M$ at pH 7.2. The sensitivities of the (M3 + MWNTs)/GCE was 2.44 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$ for rutin and 7.19 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$ for quercetin without interference.

Effects of Whole Body Ultraviolet -Light Irradiation on Serum Protein in Snake Head, Ophicephalus argus(CANTOR) (가물치의 혈청단백질에 미치는 자외선전신조사의 영향)

  • 남상열;이재문;최미자;이향순
    • The Korean Journal of Zoology
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    • v.7 no.2
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    • pp.6-12
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    • 1964
  • The present paper deals with the changes in serum protein fraction, total serum protein, hematocrit, red blood cell, haemoglobin, and weight of liver or kidney : body weight ratio of irradiated and non-irradiated snake head, Ophicephalus argus(CANTOR) . Irrardiation doses are 2537$\AA$-7 minutes (7M) and 2537$\AA$-15 minutes (15M). Serum electrophoretic patterns showed a marked decrease in albumin fraction at 1, 3, 9, 12 and 15 days on 7M group and 15 M group. On both experimental groups percentage increases in $\alpha$1 and $\beta$ fractions occurred at different time periods in general but are interpreted as only apparent changes accompanying the greater albumin fall. ${\gamma}$-Globulin decreased at 1, 6 and 15 days on 7M group. and at 1, 3, 6 and 9 days on 15M group. Also, A/G ratio was significantly low in groups subjected to above conditions as compared to the controls. A/G ratio decreased at 3 and 15 days remarkably on both groups. On the average, the reductions in the A/G ratio were not proportional to themagnitude of ultraviolet-light. Total protein of serum changed according to suggestive changes in electrophoretic patterns of serum . Total protein of serum declined at 1, 3, 6, 9 and 12 day periods on 7M groups and at 1, 3, 9, 12 and 15 day-periods on 15 M group, and increased approaching control values at 15 and 18 day-periods on both groups. Hematocrit increased remarkably at 1, 3, 6 and 12 days and decreased at 9 and 15 days on 7M group, and increased throughout the sampling period on 15M . Red blood cell decreased throughout the sampling period and increased slightly at 6 days on 7 M group and decreased at 1, 6, 9, 15 and 18 days and increased remarkably at 3 and 12 days on 15M group. Hemoglobin decreased remarkably at 1 day-period and increased at other days on both groups. The liver weight was not remarkably changed after whole body irradiation on both groups. and kidney was increased from 1st day on 7M group and 3 rd day on 15M group respectively. It appears that changes in electropphoretic patterns of serum, A/G ratio, total protein of serum, hematocrit, red blood cell, hemoglobin, and liver or kidney weight act to the detriment of the animal following non-ionizing irradiation.

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Analysis of Temperature and Humidity Distributions according to Arrangements of Air Circulation Fans in Single-span Tomato Greenhouse (토마토 단동온실에서 공기순환팬 설치 방법에 의한 온실 내 온습도 분포 분석)

  • Lee, Tae Seok;Kang, Geum Choon;Paek, Yee;Moon, Jong Pil;Oh, Sung Sik;Kwon, Jin Kyung
    • Journal of Bio-Environment Control
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    • v.25 no.4
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    • pp.277-282
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    • 2016
  • This study was aimed to investigate the effect of air-circulation fans on air temperature and relative humidity in a single-span tomato greenhouse (W: 7m, L: 25m, H: 3.2m). According to standard of fan layout by ASAE (1997), a total of 10 fans were bilaterally arranged in 2 rows in the experimental greenhouse. The distributions of air temperature and relative humidity were measured from 6 pm to 8 am under different conditions, with and without fans. The measurement heights were 0.7m, 1.7m and 2.7m. Under the condition of "fans off", the spatial differences of air temperature and relative humidity between upper and lower sides were $1.7^{\circ}C$ and 10.8%, respectively. The operation of 10 fans showed their differences to $0.1^{\circ}C$ and 3.2%. The number of fans and installation direction were evaluated their performance on reducing the spatial variation of air temperature and relative humidity. The experimental layouts were 5 and fans in 2 rows (bilaterally) and 10 fans in the one (same) direction. Under the condition of "6 fans on" and "5 fans on", the spatial differences of air temperature and relative humidity between upper and lower side were $0.3^{\circ}C$, 3.4% and $0.3^{\circ}C$ and 4.0%. The operation of 10 fans in the one direction reduced their differences to $0.5^{\circ}C$ and 4.9%. The overall findings of this study showed that there was no significant differences under each condition. Therefore, this study suggested that it is more economic and effective to install five fans in 2 rows (bilaterally) in the greenhouse (W: 7m, L: 25m, H: 3.2m).

Studies on the Mechanism of Contraction by Substance P in Rabbit Ileum (Substance P에 의한 가토 회장평활근의 수축기전에 대한 연구)

  • Jo, Se-Hun;Jung, Jin-Sup;Lee, Sang-Ho
    • The Korean Journal of Physiology
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    • v.18 no.2
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    • pp.151-162
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    • 1984
  • The mechanism of the contractile response of longitudial muscle of rabbit ileum to substance P (SP) has been investigated. The contractions in rabbit ileum under various conditions were recorded isometrically The following results were obtained. 1) The contractions by SP increased according to concentrations. SP·induced contraction was not sustained but faded rapidly at $10^{-7}M$. The response to the commutative addition of SP was decreased in comparison to the response to separate administration of each concentration . 2) The response to $10^{-8}M$ SP after 5 min application cf $10^{-7}M$ SP was increased with increasing the time interval between the administration of $10^{-7}$ and $10^{-8}M$ SP. 3) The treatment of rabbit ileum by $10^{-7}M$ SP for 5 min didn't decrease the response to $10^{-6}M$ acetylcholine. 4) $10^{-6}M$ atropine had no effect of the contractile response to $10^{-7}M$ SP. The response to $10^{-7}M$ SP was normal or subnormal in the presence of 3 mM tetraethylammonium(TEA). 5) 100k solution, $10^{-4}M$ ouabain, and Na-free solution inhibited the response to $10^{-8}M$ SP and 3 mM TEA completely, and to $10^{-7}M$ SP incompletely. 3 mM TEA induced a considerable contraction in K-free solution, but $10^{-8}M$ SP didn't induce the contraction. $10^{-6}M$ norepinephrine decreased the contractile responses to SP and TEA. 6) The contractile response to $10^{-7}M$ SP was dependent on the extracellular $Ca^{2+}$ concentrations to 1.8 mM. 7) The contractile response to $10^{-7}M$ SP remained 15% of the maximal response after bathing the ileum in a Ca-free solution for 2.5 min. 8) The responsiveness to SP was completely lost within 10 min of bathing in Ca-free solution, but was restored by the exposure to $Ca^{2+}$. The restorative effect of $Ca^{2+}$ depended on the concentration of $Ca^{2+}$, and on time for which the tissue exposed to this $Ca^{2+}$ concentration. These results suggest that there are two mechanisms of the action by which the low concentrations of substance P causes the contraction of intestinal smooth muscle: the reduction of K conductance and a mechanism dependent on the extracellular $Ca^{2+}$, and that high concentration of SP may elicit a contraction by releasing $Ca^{2+}$ from an intracellular store, which is not as sensitive to removal of extracellular $Ca^{2+}$ or as easily accessible to EGTA as the extracellular space of the muscle. The location of this store is not known; it may be associated with the internal side of the cell membrane.

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In vitro Response of the MPO System of the Clam, Coelomactra antiquata with Exposure to Cytochrome P450 Inducers (Cytochrome P450 유도제에 노출시킨 명주조개 (Coelomactra antiquata) 약물대사효소계의 in vitro 반응)

  • Jeon Joong Kyun;Lee Mee Hee;Shim Won Joon;Lee Soo Hyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.2
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    • pp.179-184
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    • 2002
  • Induction of cytochrome P45O (CYP) and 7-etholqresorufin-O-deethylase (EROD) in the microsome exposed to 3-methylcholan-throne (MC), $\beta$-naphthoflavone (BNF) and phenobarbital-Na (PB) was investigated, Microsome was isolated from digestive gland of clam (Coelomactra antiquata) and then exposed to each chemical in concentration range of 0.1 to 1.0 mM for 7 hours. The CYP content and EROD activity in the microsome exposed to each chemical significantly increased compared to the control group. The overall CYP and EROD induction potency was in order of MC>BNF>PB. The induction response of EROD was two times higher than that of CYP level in the microsome exposed to MC, but the induction response of EROD was slightly higher than that of CYP level in BNF and PB exposure groups.

Effect of Benzo(k)fluoroanthene and Genistein on CYP1A1 Gene Expression in Human Breast Cancer MCF-7 Cells. (사람 유방암 세포 MCF-7에서 Benzo(k)fluoroanthene과 genistein이 CYP1A1 유전자 발현에 미치는 영향)

  • Yang, So-Yeon;Min, Kyung-Nan;Shin, Yhun-Yhong
    • Environmental Mutagens and Carcinogens
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    • v.24 no.3
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    • pp.128-136
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    • 2004
  • CYP1A1 is known to be inducible by xenobiotic compouds such as polyciclic aromatic hydrocarbons(PAHs) and 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). These chemicals have been identified worldwide and can have a significant impact on the human health and well being of human and wildlife. Given these issues, the detection and quantification of these chemicals in biological, environmental and food samples is important. First, we investigated the effect of on CYP1A1 promoter activity, 7-ethoxyresorufin-O-deethylase(EROD) activity and CYP1A1 mRNA expression induced by benzo(k)fluoranthene(B(k)F) in MCF-7 cells. We found that B(k)F significantly up-regulates the level of CYP1A1 prompter activity, EROD and CYP1A1 mRNA. When cells were treated with genistein, it was not changed that EROD and CYP1A1 mRNA, compared to that of control. However, genistein inhibited the B(k)F-induced CYP1A1 promoter activity and mRNA level at high concentration. Furthermore, in this study, effects of HDAC(histone deacetvlase) inhibitors on human prostate cancer cells proliferation were examined. HC-toxin, SAHA and TSA inhibited cell proliferation in PC3 cells. A novel HDAC inhibitor, IN2001 also suppressed the growth of PC3 cells. And IN2001 and SAHA increased S phase and G2/M phase at 12 hrs treatment but cells were arrested G0/G1 phase at 45 hrs treatment. The HC-toxin treatment for 24 hrs and 48 hrs increased G0/G1 at low concentration ($0.1\mu\textrm{m}$) but increased G2/M at more than concentration of $1\mu\textrm{m}$. TSA increased G2/M phase. These findings height the possbility of developing HDAC inhibitors as potential anticancer therapeutic agents for the treatment of prostate cancer.

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Composition of Benthic Diatom Species and Biomass in the Tidal Flat of Southwestern Coast in Korea (한국 남서해안 갯벌의 저서돌말류 종조성 및 생물량)

  • Kong, Se Hoon;Shin, Yoon Keun
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.610-619
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    • 2018
  • This study was carried out in August and September 2016 to investigate the species composition and biomass of benthic diatoms in Yubu Island, Gomso Bay, Imja Island, and Yeoja Bay tidal flat of Southwestern coast. There were 181 species of 48 genera in Yubu Island tidal flats, 194 species of 51 genera in Gomso Bay tidal flats, 224 species of 64 genera in Imja Island tidal flats and 188 species of 56 genera in Yeoja Bay tidal flats. A total of 274 species of 70 genera appeared. Pennales appeared more widely than Centrales and were dominated by Paralia sulcata and Navicula spp. Biomass of Yubu Island tidal flat ranged from $18.8-136.1mg\;m^{-2}$, $31.9-215.7mg\;m^{-2}$ in Gomso Bay, $2.9-120.2mg\;m^{-2}$ in Imja Island and $10.1-147.7mg\;m^{-2}$ in Yeoja Bay. The range of total biomass from 4 areas was $2.9-215.7mg\;m^{-2}$. The concentration of phaeopigment and degradation product of chlorophyll-a was $1.7-470.8mg\;m^{-2}$ in Yubu Island tidal flat, $52.3-277.2mg\;m^{-2}$ in Gomso Bay, $0.6-78.9mg\;m^{-2}$ in Imja Island and $39.1-346.3mg\;m^{-2}$ in Yeoja Bay. Compared with the results reported in this study area and the domestic tidal flats, it cannot be directly compared and evaluated due to various factors such as the timing of the survey, the frequency of the survey, the analysis method and geography. The southwestern coast of Korea has a variety of benthic diatoms and the high concentration of chlorophyll-a is the main determinant of primary productivity.

The Fabrication of High Strength 7XXX Aluminum Alloy Powders by Centrifugal Disc Atomization (원심분무법에 의한 고강도 7XXX 알루미늄 합금 분말의 제조)

  • Lee, Tae-Hang;Im, Seong-Moo;Cho, Sung-Suk
    • Journal of Korea Foundry Society
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    • v.10 no.6
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    • pp.528-537
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    • 1990
  • 7XXX aluminum alloy powders produced by the self-manufactured rotating disc atomizer were investigated to determine the influence of the atomization parameters on the particle size distributions in air atmosphere. The particle size distributions are almost always bimodal with the dominant mode on the large particle size. Average powder size of 7XXX aluminum alloy is $74/{\mu}m~125/{\mu}m$ when melt is poured with the rate of 9g /sec at 730$^{\circ}C$ on a rotating disc of 30㎜ diameter at 6300rad/sec. The mass of finer particle increased when disc diameter, angular velocity, pouring temperature increased and pouring rate decreased. The powder shapes of bimodal change from acicular to tear-drop and from tear-drop to ligament with increasing powder size. Powder shape was determined by the atomization mechanism and oxidation in liquid state. Microstructure of powders appeared to be cell and cellular dendrite. The SDAS of Al-7.9wt%Zn-2.4wt%Mg-1.5wt%Cu-0.9wt%Ni Powders is $0.8{\mu}m~1.0{\mu}m$ for the powders of $size+44{\mu}m~53{\mu}m$ and $1.6{\mu}m∼1.8{\mu}m$ for the powders of $size+105{\mu}m~125{\mu}m$, repectively.

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