• Analytical Science and Technology
• /
• v.16 no.3
• /
• pp.198-205
• /
• 2003

The ammonium salt of nitrosophenylhydroxylamine, called cupferron, has been used not only as the ligand but also as an oxidizing agent for adsorptive catalytic stripping voltammetry (AdCtSV). Cyclic voltammetry was used for elucidating the electrochemical behavior of Os-cupferron complex in 1 mM phosphate buffer. The optimal conditions for osmium analysis were found to be 1 mM phosphate buffer solution (pH 6.0) containing 0.1 mM cupferron at scan rate of 100 mV/s. By using the plot of reduction peak currents of linear scan voltammograms vs. osmium concentration, the detection limit was $1.0{\times}10^{-7}M$.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.28 no.3
• /
• pp.330-336
• /
• 2014

This study aimed at examining the anti-inflammatory effects of Scutellariae Radix & Lonicerae Caulis water extract(SC). RAW 264.7 mouse macrophage cells were treated with $25{\sim}200{\mu}g/m{\ell}$ SC for 24 hours. Cell viability was then measured using MTT assays. The nitric oxide(NO) production and the creation of several cytokines in LPS-stimulated RAW 264.7 cells were investigated. SC inhibited significantly increasing the production of NO in LPS-induced RAW 264.7 cell at the density of 25, 50 and $200{\mu}g/m{\ell}$. SC inhibited significantly the TNF-${\alpha}$ of the RAW 264.7 cell induced by LPS at the density of $50{\mu}g/m{\ell}$. SC inhibited significantly the MIP-$1{\alpha}$ of the RAW 264.7 cell induced by LPS at the density of 25, 50 and $100{\mu}g/m{\ell}$. SC inhibited significantly the MIP-$1{\beta}$, MIP-2 at the density of 50, $100{\mu}g/m{\ell}$ in the RAW 264.7 cell increased by LPS, respectively. SC did not affect the production levels of VEGF in RAW 264.7 cell. As a result, SC significantly inhibited the inductions of MIP-$1{\alpha}$, MIP-$1{\beta}$, MIP-2 and NO in LPS-induced RAW 264.7 cell without causing the toxicity. These results signify that SC has anti-inflammatory effects on controlling the over inflammatory reaction on the RAW 264.7 cell.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.40 no.11
• /
• pp.34-41
• /
• 2003

In this paper, 60GHz band transmitter and receiver for wireless LAN are designed and implemented using the broband amplifier and mixer fabricated by standard 0.1${\mu}{\textrm}{m}$ MIMIC process of MINT. Output power and gain of the RF transmitter are 0 ㏈m and 1.7㏈, respectively. Noise figure and gain of the receiver are 4.2㏈ and l5.7dB, respectively. Considering the sensitivity and LOS test, this system can communicate with BER of below than 10$^{-6}$ at a distance more than 35m. DSSS, which is strong for concealment and disturbance, is adopted.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.7 no.1
• /
• pp.125-134
• /
• 2012

This study examines how the concentration of Fine Particles(PM10: Fine Particles of under Diameter $10{\mu}m$) included in the air influences on the satellite signal transmission frequency band. For the study of satellite signal attenuation rate by the concentration of Fine Particles, the concentration of Fine Particles classified 7 levels, below $50{\mu}g/m^3$, below $100{\mu}g/m^3$, below $200{\mu}g/m^3$, below $400{\mu}g/m^3$, below $800{\mu}g/m^3$, below $1,600{\mu}g/m^3$, below $3,200{\mu}g/m^3$. After then, it analyzed how much each concentration influenced on the satellite signal transmission frequency band.

• Food Science and Preservation
• /
• v.10 no.3
• /
• pp.293-296
• /
• 2003

This study was carried out to obtain physical characteristics of paper board and to measure bulge of 1,000 mL carton pak by different storage conditions. Water holding capacity of paper boards were mean of 5.6～7.0％, stiffness of machine direction(MD) was 243.3～266.7 g/cm and cross direction(CD) was 99.2～109.2 g/cm, respectively. The weight of 1,000 mL carton pak were in the range of 29.17～31.26 g. The bulge of carton paks were 6.33～6.93 mm after 7 days at 6$^{\circ}C$ storage, 7.26～8.56 mm after 7 days at 10$^{\circ}C$ and was 8.66 mm after 7 days at 10$^{\circ}C$ treated with 1 hour heat shock per day, respectively. According to this result, the bulge length of 1,000 mL carton pak was showed increased pattern upon increasing the storage temperature and the order was 6$^{\circ}C$ < l0$^{\circ}C$ < 10$^{\circ}C$＋ heat shock. To prevent bulge occurence, it was thought to control storage temperature and humidity of paper board or carton pak and to minimize their shock on cold chain system after filling process.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.5
• /
• pp.956-964
• /
• 2017

Compactin and pravastatin are competitive cholesterol biosynthesis inhibitors of 3-hydroxy-3-methylglutaryl-CoA reductase and belong to the statin drugs; however, the latter shows superior pharmacokinetic characteristics. Previously, we reported that the bacterial P450, CYP105D7, from Streptomyces avermitilis can catalyze the hydroxylation of 1-deoxypentalenic acid, diclofenac, and naringenin. Here, we demonstrate that CYP105D7 could also catalyze compactin hydroxylation in vitro. In the presence of both bacterial and cyanobacterial redox partner systems with an NADPH regeneration system, the reaction produced two hydroxylated products, including pravastatin (hydroxylated at the C6 position). The steady-state kinetic parameters were measured using the redox partners of putidaredoxin and its reductase. The $k_m$ and $k_{cat}$ values for compactin were $39.1{\pm}8.8{\mu}M$ and $1.12{\pm}0.09min^{-1}$, respectively. The $k_{cat}/K_m$ value for compactin ($0.029min^{-1}{\cdot}{\mu}M^{-1}$) was lower than that for diclofenac ($0.114min^{-1}{\cdot}{\mu}M^{-1}$). Spectroscopic analysis showed that CYP105D7 binds to compactin with a $K_d$ value of $17.5{\pm}3.6{\mu}M$. Molecular docking analysis was performed to build a possible binding model of compactin. Comparisons of different substrates with CYP105D7 were conclusively illustrated for the first time.

• Journal of IKEEE
• /
• v.11 no.1 s.20
• /
• pp.15-23
• /
• 2007

This paper describes a dual frequency synthesizer designed in a 0.2$\mu$m CMOS technology for wireless LAN applications. The design is focused mainly on low-power characteristics. Power dissipation is minimized especially in VCO and prescaler design. The designed synthesizer includes all building blocks for elimination of external components, other than the crystal. Its operating frequency can be programmed by external data. It operates in the frequency range of 2.3GHz to 2.7GHz (RF) and 250MHz to 800MHz (IF) and consumes 5.14mA at 2.5GHz and 1.08mA at 0.5GHz from a 2.5V supply. The measured phase noise is -85dBc/Hz in-band and -105dBc/Hz at 1MHz offset at IF band. The die area is 1.7mm$\times$1.7mm.

• The Journal of Korean Physical Therapy
• /
• v.14 no.2
• /
• pp.172-180
• /
• 2002

The purpose of this paper was to make an analysis of anaerobic power in professional female basketball players using the Wingate Test Method with bicycle ergometer. Twenty-three subjects(age $21.6\pm2.8years$, body height $178.0\pm7.4cm$, body weight $70.3\pm7.4kg$) were selected from professional female basketball team whose careers were over 10years and participated in this investigation. Each subject peformed a Wingate anaerobic power test to determine total work, peak power, mean power, fatigue index and blood lactate concentration. The following were obtained from result data analysis; 1. The Total Work of athletes was a $1128.7\pm120.6watt$ 2. The Peak Power of athletes was a $449.5\pm53.1watt$ 3. The Mean Power of athletes was a $369.1\pm39.4watt$ 4. The Fatigue Index of athletes was a $33.5\pm6.9\%$ 5. The blood lactate concentration was $1.85\pm0.85mM/L$ at the normal state and $3.16\pm1.53mM/L$ at the after Wingate test. The blood lactate concentration was $6.96\pm0.81mM/L$ after 3 minute and $6.95\pm1.05mM/L$ after 5 minutes.

• The Korean Journal of Pesticide Science
• /
• v.16 no.2
• /
• pp.171-177
• /
• 2012

Pepper seedlings of 6-leaf stage were inoculated with cell suspension of Xanthomonas euvesicatoria 1523 7 days after the application of Bion-M by soil-drenching. Disease severity in the treatment with 20 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, with which Bion-M was composed, was 19%, whereas that of the untreated control was 75%. The resulting control value of acibenzolar-S-methyl was calaulated as much as 74.7%. The control value of acibenzolar-S-methyl was dependent with the applied concentrations and ranged 29.3% to 49.3% at 4.0 and 0.8 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, respectively. Phytotoxicity was observed at 20.0 ${\mu}g\;mL^{-1}$, as lower leaves became to be yellowed and defoliated. The cell density of inoculum suspension of X. euvesicatoria 1523 affected the control value of acibenzolar-S-methyl. With optical density (O.D.) of 0.5 the control value of 4.0 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl was 86.0%. However, the control value improved as high as 97.8% at the O.D. value of 0.1. The control value was 75.0% in adult plant of pepper, when acibenzolar-S-methyl was treated by soil-drenching 7 days before inoculation with cell suspension of X. euvesicatoria 1523. The control effect of acibenzolar-S-methyl on pepper bacterial spot was obtained in pepper field, showing that the control value at 10.0 ${\mu}g\;mL^{-1}$ was 71.2%.

• The Korean Journal of Mycology
• /
• v.31 no.1
• /
• pp.40-43
• /
• 2003

Symptoms similar to anthracnose were observed on Amaranthus mangostanus in Sancheon-gun, Gyeongnam province, where the plants were autogenously formed community. The symptoms were appeared in stem and spread, eventually whole plants died. Mycelial colony of the isolate was whitish gray to dark gray on potato dextrose agar. Conidia were single celled, colorless, cylindrical and measured as $10.5{\sim}21.7{\times}3.8{\sim}6.0{\mu}m$. Appressoria were dark brown, ovate to obovate and sized as $5.6{\sim}13.7{\times}4.6{\sim}11.4{\mu}m$. Perithecia were brown to black in color and shaped as globose to obpyriform and sized as $79.7{\sim}286.7{\mu}m$. Asci had eight ascospores and sized as $47.7{\sim}89.7{\times}8.1{\sim}13.3{\mu}m$. Ascospores were slightly curved at the center cylindrical, fusiform and measured $9.3{\sim}20.3{\times}4.6{\sim}6.3{\mu}m$. Optimum temperature for growth was $30^{\circ}C$. On the basis of morphological characteristics and pathogenicity test to host plants, the fungus was identified as Glomerella cingulata. This is the first report on the Anthracnose of Amaranthus mangostanus caused by Glomerella cingulata in Korea.