• Restorative Dentistry and Endodontics
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• v.47 no.4
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• pp.42.1-42.11
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• 2022

Objective: This study investigated the effects of various concentrations of sodium hypochlorite (NaOCl) on human whole-blood clotting kinetics, the structure of the blood clots formed, and transforming growth factor (TGF)-β1 release. Materials and Methods: Human whole blood was collected from 5 healthy volunteers and divided into 4 groups: CG (control, 0.5 mL of blood), BN_0.5 (0.5 mL of blood with 0.5 mL of 0.5% NaOCl), BN₃ (0.5 mL of blood with 0.5 mL of 3% NaOCl), and BN_5.25 (0.5 mL of blood with 0.5 mL of 5.25% NaOCl). The effects of NaOCl on clotting kinetics, structure of fibrin and cells, and release of TGF-β1 were assessed using thromboelastography (TEG), scanning electron microscopy (SEM), and enzyme-linked immunosobent assay, respectively. Statistical analysis was conducted using the Kruskal Wallis and Mann-Whitney U tests, followed by the post hoc Dunn test. A p value < 0.05 indicated statistical significance. Results: The blood samples in BN_0.5 and BN₃ did not clot, whereas the TEG of BN_5.25 showed altered clot formation. Samples from the CG and BN₃ groups could only be processed with SEM, which showed that the latter lacked fibrin formation and branching of fibers, as well as clumping of red blood cells with surface roughening and distortion. TGF-β1 release was significantly highest in BN₃ when all groups were compared to CG (p < 0.05). Conclusions: Each concentration of NaOCl affected the release of TGF-β1 from blood clots and altered the clotting mechanism of blood by affecting clotting kinetics and cell structure.

• Culinary science and hospitality research
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• v.23 no.1
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• pp.28-36
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• 2017

This study made macaroon with the addition of Helianthus tuberosus L. powder so as to increase the application of Helianthus tuberosus L. powder (HTP) to food products and look into the quality characteristics depending on an addition ratio. Helianthus tuberosus L. powder 0%, 0.5%, 1.0%, 1.5%, and 2.0% were added, respectively, to make macaroon. As Helianthus tuberosus L. powder was added more, the moisture content tended to decrease more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, lightness and yellowness tended to reduce more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, the content of total phenol compounds and DPPH radical scavenging activity were significantly increased (p<0.001). In terms of texture analysis, hardness, gumminess, cohesiveness and chewiness were significantly increased, as the Helianthus tuberosus L. powder was increased (p<0.05). In terms of sensory test, regarding taste, 0.5% HTP group had the highest points. And as color, flavor, and texture were improved, 1.5% HTP showed the most desirable result in terms of overall acceptability. Results, if Helianthus tuberosus L. powder is added for cooking macaroon, it seems to increase anti-oxidation function and develop functional macaroon with excellent sensory factors.

• Journal of the Korean Geotechnical Society
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• v.19 no.5
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• pp.311-317
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• 2003

The swelling index of clayey soil was examined by constant rate of strain(CRS) consolidation test. Four kinds of strain rate were applied during unloading. The strain rates are l/l, l/5, 1/10, l/l 5 of loading. The strain rates during loading are 0.05%/min and 0.03%/min. From the test results using standard consolidometer, the swelling indexes were much similar values in case of 1/5 or 1/10 of the strain rate during loading stage. In the relation between effective stress and excess pore water pressure ratio, it was found that the existence of cross point and the stress level can be separated into two zones according to the swelling index.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.993-1000
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• 2023

Recently, enviromentally friendly natural substances derived from plants have been attracting attention as cosmetic materials, and research on various physiological activities of natural substances is being actively conducted. This study investigated the antioxidant, anti-inflammatory, moisturizing, and antibacterial effects of three types of extracts of mixtures containing different mixing ratios, Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens, known to have various physiological activities. The mixing ratio is 7 conditions (M1, 1:1:1; M2, 0.5:1.5:1; M3, 1.5:0.5:1; M4, 0.1:0.95:0.95; M5, 0.5:0.5:2; M6, 0.95 :1.95:0.1; M7, 1.45:0.1:1.45), and the optimal mixing ratio was confirmed for use as a cosmetic material. DPPH and ABTS radical scavenging activities showed scavenging abilities of 75.37% and 99.19%, respectively, at 1,000 ㎍/mL of M6. At a concentration of 200 ㎍/mL of M6, it showed 50% of nitric oxide production inhibition compared to the lipopolysaccharide-treated that induced an inflammatory response. It was confirmed that M3 and M6 produced hyaluronic acid 1.47 and 1.49 times higher than the control at a concentration of 50 ㎍/mL, respectively. Through the disc diffiusion test, the clear zone was 9.75 mm at 8 ㎍/mL of M6, confirming the inhibition of growth of staplylococcus aureus strain. Based on the above results, it is believed that the mixed extract of Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens can be used as a functional natural material for cosmetics.

• Korean Journal of Ecology and Environment
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• v.39 no.3 s.117
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• pp.340-351
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• 2006

To reveal physicochemical factors contributing to variation of phytoplankton communities, the study was carried out biweekly at 6 stations from Feb. 2004 to Feb. 2005 in the lower part of the Han River, Korea. As results, water temperature was changed from $0.3^{\circ}C$ to $26.6^{\circ}C$, pH: 6.6${\sim}$9.1, DO: 1.89${\sim}$22.23 mg $L^{-1}$, BOD: 0.38${\sim}$9.20 mg $L^{-1}$, COD: 1.4${\sim}$15.2 mg $L^{-1}$, Conductivity: $62.5{\sim}500.0\;{\mu}s\;cm^{-1}$, SS: 3.00${\sim}$159.3 mg $L^{-1}$, and Chl a $1.7{\sim}71.3\;{\mu}g\;L^{-1}$. Phytoplankton standing crops ranged from min. $3.6{\times}10^2\;cells\;mL^{-1}$ (July 2004, St. 3) to max. $2.3{\times}10^4\;cells\;mL^{-1}$ (Feb. 2005, St. 6), and mean of those varied from $5.9{\times}10^3\;cells\;mL^{-1}$in spring, $2.1{\times}10^3\;cells\;mL^{-1}$ in summer, $4.1{\times}10^3\;cells\;mL^{-1}$ in autumn and $8.5{\times}10^3\;cells\;mL^{-1}$ in winter, respectively. In order to investigate factors influencing the total phytoplankton standing crops a multiple regression analysis was adopted for the correlation between standing crops and environmental factors. The coefficient of determination ($R^2$) value of the regression was 0.465, it showed that environmental factors which predominantly influenced phytoplankton standing crops were water temperature, COD, $NO_2-N$, $PO_4-N$, Discharge and pH. six stations could be divided into 3 groups based on similarity index in terms of environmental factors. In ANOVA analysis for physicochemical and biological factors, water temperature, chlorophyll a, silicate, phytoplankton standing crops were the same group differed little from stations. However, Station 1and 2 were grouped followed in dissolved oxygen, conductivity, COD, nitrite, nitrate, ammonia and phosphate, and Station 3, 4 and 5 were followed in dissolved oxygen, conductivity, pH and phosphate.

• Journal of Bio-Environment Control
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• v.19 no.2
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• pp.97-108
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• 2010

The present study was conducted to investigate photosynthetic responses (Pn), chlorophyll fluorescence (Pe), chlorophyll contents of Allium victorialis var. platyphyllum, Ligularia fischeri, and Ligularia stenocephala growing at four different fertilizing schemes (non-fertilizing and 2.5 $g{\cdot}l^{-1}$, 5 $g{\cdot}l^{-1}$, 10 $g{\cdot}l^{-1}$ fertilizing). Three wild vegetables showed outstanding Pn and Pe at 5 $g{\cdot}l^{-1}$ fertilizing treatment. 10 $g{\cdot}l^{-1}$ of fertilizer, however, proved to be too much eventually leading to declined growth. A. victorialis var. platyphyllum showed good Pn and Pe under shade treatment whereas Pn and Pe of L. fischeri, and L. stenocephala showed the opposite tendency. The chlorophyll contents of the three wild vegetables showed the highest measurement ranging between 11.70~24.36 $mg{\cdot}g^{-1}$ when treated with 5 $g{\cdot}l^{-1}$ of fertilizer. Also it was showed that there were more chlorophyll contents under shade treatment as opposed to full sun. These results showed that 5 $g{\cdot}l^{-1}$ fertilizing treatment is optimal fertilizing of three wild vegetables and fertilizing and light intensity controlling is very important for productivity of vegetables.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.264-269
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• 2002

The production of exopolysaccharide from Zoogloea ramigera 115SLR in batch culture was affected by carbon sources, rifampicin concentration, inoculum size and cell density of starter culture. The increase of organic nitrogen concentration and cell density in defined medium by adding starter culture resulted in higher production of exopolysaccharide (EPS) within 2 days. Glucose and lactose as carbon sources showed EPS production of 10.7 gk and 10.5 ga, respectively. Higher EPS production was obtained with 2.5% (w/v) glucose. White sugar, brown sugar and galactose produced less than 10 gt of EPS. The optical density of starter culture affected on EPS yield, producing over 10 gt of EPS in the range of 1.0-1.7. In the presence of rifsmpicin, Z. ramigera 115SLR produced EPS of 12.9 gt. Molecular weight of EPS produced with/without rifampicin was determined with 1.367$\times$106 and 1.711$\times$106 g/mol using HPSEC-MALLS-RI, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1115-1119
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• 2001

We prepared a functional red soybean curd (RS) from Ang-Khak according to various concentration (RS1 : red rice powder 0.1 g/10 mL, RS2: 0.2 g/10 mL, RS3: 0.3 g/10 mL, RS4: 0.4 g/10 mL, RS5: 0.5 g/10 mL, CS: control soybean curd). During the storage period of red soybean curd, pH, acidity, and microbial counts showed a minimum change in RS5. And in the case of color, all groups have slightly increased in yellowness except RS1. In the case of texture, hardness, gumminess, and springiness appeared to be increased for a few days and then fell down. As water drained out of soybean curd during storage at 1$0^{\circ}C$, it became so stiff and then rotten that the hardness of soybean curd increased in the early stage and then decreased after all. After a week, we could find a better preservative effect of RS than CS. Resultly, we need more efforts to prolong the shelf-life of soybean curd with applying the functionality of Ang-Khak.

• Analytical Science and Technology
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• v.15 no.2
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• pp.172-179
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• 2002

Determination were performed for the presence of three common environmental endocrine disrupting compounds (EDC), namely nonylphenol (NP), octylphenol (OP), di(2-ethylhexyl)phthalate (DEHP) in rainwater, distilled water, millipore water, groundwater, tap water, and bottle water in market, using OASIS cartrige extraction and GC/MS-SIM. Octylphenol was not detected in any water samples. The order of nonylphenol concentrations were increased in rainwater < millipore water < distilled water < groundwater < tap water < bottle water. The concentration of nonylphenol in bolltle water was $0.44{\mu}g/L$(ppb) at highest. Di(2-ethylhexyl)phthalates were detected in every water samples, and the concentrations were to be $1.70{\sim}2.92{\mu}g/L$ in rainwater, $8.7{\sim}31.7{\mu}g/L$ in distilled water, $0.6{\sim}5.6{\mu}g/L$(mean: $2.5{\pm}0.3{\mu}g/L$) in millipore water, and that were very stable values than other waters, $1.1{\sim}6.0{\mu}g/L$ in groundwater at near Jeonju, $3.1{\sim}5.8{\mu}g/L$ by means in tap water, $0.5{\sim}67.6{\mu}g/L$ in bottle water from market. The concentration of DEHP in bottle water samples from market were in higher than USEPA regulation at over 40%.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.11
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• pp.5206-5210
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• 2012

We studied the effect of food concentration on grazing, growth and fecundity of cyclopoid copepod Paracyclopina nana. Marine phytoplankton Tetraselmis suecica was used as a livefood for the copepod. Six stage compositions were used and food concentrations for the experiment were 0.5, 1, 2, 5, 10, 20, 40, 60 and $80{\times}10^4$ cells/mL. Range of food concentrations with 0.2, 0.5, 1, 2, 3 and $4{\times}10^4$ cells/mL were used for nauplii production experiment. Grazing rates of P. nana in all developmental stages on the different concentrations were increased with increasing diet concentration. While the growth of nauplius was not affected by increase of food concentration, food concentration outside of $1{\times}10^4$ to $5{\times}10^4$ cells/mL range negatively affected that of copepodite. Daily nauplii production was increased with increasing food concentration but $3{\times}10^4$ and $4{\times}10^4$ cells/mL treatments were not significantly different with $2{\times}10^4$ cells/mL treatment. As a result, optimum concentration of T. suecica for mass culture of P. nana was considered to be 5,000 cells/mL for nauplius stage, 10,000 cells/mL for copepodite stage and adult male, 20,000 cells/mL for adult female, respectively.