• Title/Summary/Keyword: 1L-5

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Studies on Removal of Heavy Metals in Irrigation Water by Water Hyacinth (부레옥잠(수초(水草)) 을 이용(利用)한 개관수중(漑灌水中) 유해중금속(有害重金屬) 제거연구(除去硏究))

  • So, Kyu-Ho;Kim, Bok-Young
    • Korean Journal of Environmental Agriculture
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    • v.11 no.2
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    • pp.133-139
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    • 1992
  • Removal of heavy metals by water hyacinth, Eichhornia crassipes Solms-Laub, was examined with two heavy metals Cd, Cu under laboratory conditions. Cd in culture solution was reducd to 0.116, 0.873, 2.015, 3.755 and 4,747 mg/L from 1, 2, 3, 4 and 5 mg/L for 24 hrs, after cultivating of water hyacinth respectively And, Cu was reduced to 0.086, 0.600, 2.174, 3.473, and 4.365 mg/L from 1, 2, 3, 4, and 5 mg/L for 24 hrs, after the cultivation, respectively. Cu was removed faster than Cd nd airating cultivation was effected higher than fixing cultivation. Removal effect of heavy metals by water hyacinth was higher in low a heavy metal cocentration than in high concentration

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Effects of Dipping Chicken Breast Meat Inoculated with Listeria monocytogenes in Lyophilized Scallion, Garlic, and Kiwi Extracts on Its Physicochemical Quality

  • Kim, Hye-Jin;Sujiwo, Joko;Kim, Hee-Jin;Jang, Aera
    • Food Science of Animal Resources
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    • v.39 no.3
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    • pp.418-429
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    • 2019
  • This study was conducted to evaluate the antioxidant and antimicrobial activities of lyophilized extracts of scallions (Allium fistulosum L., SLE), garlic (Allium sativum, GLE), and gold kiwi (Actinidia chinensis, GKE) and their effects on the quality of chicken breast meat inoculated with L. monocytogenes during storage for 9 days at $4^{\circ}C$. The lowest minimum inhibitory concentration and minimum bactericidal concentration (25 and 100 mg/mL, respectively) against L. monocytogenes were observed for SLE and GLE, respectively. GKE had the lowest half-maximal inhibitory concentration ($IC_{50}$) for 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity (5.06 mg/mL). The pH values of meat inoculated with L. monocytogenes and dipped in 1% SLE (LSLE), 1% GLE (LGLE), or 1% GKE (LGKE) were lower than that of the control on day 3 of storage (p<0.05). The initial population of L. monocytogenes in meat was 4.95-5.01 Log CFU/g. However, the population in the LSLE (5.73 Log CFU/g) was lower than that in the control (6.23 Log CFU/g) on day 5 (p<0.05). The volatile basic nitrogen value of the LSLE (19.90 mg/100 g) was lower than that of the control (24.38 mg/100 g) on day 7 (p<0.05). Moreover, treatment with SLE resulted in the maintenance of meat quality and reduced the population of L. monocytogenes on the meat. Thus, SLE may be used as an alternative natural and environmentally friendly sanitizer for reducing L. monocytogenes contamination in the chicken meat industry.

Synthesis of L-1,3-Dioxolane and L-1,3-Oxathiolane Pyrimidine Nucleosides (L-1,3-Dioxolane 및 L-1,3-Oxathiolane 피리미딘 뉴크레오사이드의 합성)

  • Hong, Joon-Hee;Park, Jeong-Ha;Song, Ji-Yeon;Choi, Bo-Gil;Chung, Byung-Ho;Kim, Joong-Hyup;Lee, Chong-Kyo;Chung, Won-Keun;Chun, Moon-Woo
    • YAKHAK HOEJI
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    • v.38 no.6
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    • pp.775-781
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    • 1994
  • $({\pm})-Dioxolane-T$ and$({\pm})-BCH-189$ are know to possess anti-HIV activities, and less to xicities compared to other dideoxynucleoside AIDS drugs. We have synthesized 34 different enantiomerically pure nucleosides by glycosylation of L-1,3-dioxolanyl acetate and L-1,3-oxathiolanyl acetate sugar moieties with uracil or 6-azauracil with alkyl chains$(C_1-C_3)$ on 5 or 6 position.

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Effect of Glutamic Acid on Polysaccharide Production from Agaricus blazei in Liquid Culture

  • Hwang, Jeong-Min;Im, Yeong-Su;Gwon, Myeong-Sang;Choe, Jeong-U;Han, Jin-Su;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.231-234
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    • 2000
  • The polysaccharide has been known to have an antitumor activity, which were extracted from the fruiting bodies, mycelia, and culture broths of Agaricus blazei. For the cell growth and the polysaccharide production, the optimal medium contained 8% glucose, 2% yeast extract, 0.1% $MgSO_4{\cdot}7H_2O$, 0.2% $KH_2PO_4$, 0.2% $CaCl_2{\cdot}2H_2O$ and 0.2% $MnSO_4{\cdot}5H_2O$. When 0.2% of glutamic acid was added at 4day, the cell concentration was 13.5 g/L and the polysaccharide production was 9.9 g/L, respectively.

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Effect of Sodium Hypochlorite for Controlling Bacterial Blotch on Pleurotus ostreatus

  • Oh, Se-Jong;Kim, Han-Kyoung;Kim, Hee-Kyu;Fermor, T.R.
    • Mycobiology
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    • v.28 no.3
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    • pp.123-126
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    • 2000
  • Sodium hypochlorite alkaline was tested against Pseudomonas tolaasii causing bacterial blotch on cultivated oyster mushroom (Pleurotus ostreatus). The minimum inhibitory concentration of sodium hypochlorite against P. tolaasii contained active chlorine (AC) at 1.4 mg/l on plate assay. The highest cultivation yield was obtained from the treatment of AC 5.7 mg/l. Treatment of sodium hypochlorite at the rate of higher than AC 11.4 mg/l resulted in reduced yields at the harvest. However, the population of total bacteria on the bed surface treated with AC 5.7 mg/l of sodium hypochlorite was maintained to some extent. Inhibitory concentration against total bacteria on the bed surface was over AC 22.8 mg/l. Mushroom mycelium was damaged and its growth strongly inhibited at the concentration of AC 200 mg/l. Mushroom caps showed yellowish symptom by chemical injury by treatments of AC 74.1 mg/l or higher. Sporocarps infected by P. tolaasii were irrevocable at any concentration of sodium hypochlorite. Routine watering with AC 5.7 mg/l from mushroom initiation to the end of picking resulted in reduced bacterial blotch incidence of 40% and 86% at two mushroom farms. The treatment resulted in higher quality mushroom production compared to that conventionally watered with tap water alone.

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Immunomodulatory Effects of Curcuma longa L. Extract in LP-BM5 Murine Leukemia Viruses-induced Murine Acquired Immune Deficiency Syndrome (면역결핍 동물모델에서 울금 주정 추출물의 면역조절 효과)

  • Kim, Ok Kyung;Yoo, Seon A;Nam, Da-Eun;Kim, Yongjae;Kim, Eun;Jun, Woojin;Hwan, Kwontack;Lee, Jeongmin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.9
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    • pp.1317-1324
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    • 2014
  • The immune system protects the body against harmful substances and infectious agents. Normally, the body can maintain a state of immune homeostasis. However, failure of immune homeostasis results in severe diseases when the immune system is defective. We investigated the immunomodulatory effect of Curcuma longa L. extract in LP-BM5 MuLV (murine leukemia viruses)-induced murine AIDS (acquired immune deficiency syndrome). Mice were divided into six groups: normal control, infected control (LP-BM5 MuLV infection), positive control (LP-BM5 MuLV infection+dietary supplement of red ginseng 200 mg/kg), CL50 (LP-BM5 MuLV infection+dietary supplement of Curcuma longa L. 20% alcohol extract 50 mg/kg), CL200 (LP-BM5 MuLV infection+dietary supplement of Curcuma longa L. 20% alcohol extract 200 mg/kg), and CL500 (LP-BM5 MuLV infection+dietary supplement of Curcuma longa L. 20% alcohol extract 500 mg/kg). We found that dietary supplementation with Curcuma longa L. 20% alcohol extract inhibited elevation of spleen, lymph node, and liver weights as well as reduction of T- and B-cell proliferation and natural killer cell activity induced by LP-BM5 MuLV infection. Moreover, Curcuma longa L. 20% alcohol extract inhibited Th1 (IL-2, IFN-${\gamma}$)/Th2 (IL-4, IL-10) cytokine imbalance and pro-inflammatory cytokine production. In conclusion, these data suggest that Curcuma longa L. has immunomodulatory effects in LP-BM5 MuLV-induced murine AIDS.

Immunomodulatory activities of crude polysaccharide fraction separated from Perilla frutescens Britton var. acuta Kudo (자소엽(Perilla frutescens Britton var. acuta Kudo) 조다당류 추출물의 면역활성 효과)

  • Byun, Eui-Hong
    • Korean Journal of Food Science and Technology
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    • v.49 no.5
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    • pp.559-566
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    • 2017
  • This aim of this study was to examine the immunomodulatory activities of crude polysaccharides from Perilla frutescens Britton var. acuta Kudo (PCP) in mouse bone marrow-derived dendritic cells (BMDC) and splenocytes. The immunomodulatory activity was determined by cell viability, nitric oxide (NO) production, cell surface marker expression (CD 80/86 and MHC class I/II), and cytokine production in BMDC, and cell viability, and cytokine production in splenocytes. Cell proliferation and cytokine production (tumor necrosis factor; TNF-${\alpha}$, interleukin (IL)-6, IL-$1{\beta}$, and IL-12) tested in BMDC were significantly increased by PCP treatment. Additionally, the cell surface markers (CD 80/86, MHC class I/II) were highly increased by PCP treatment. For cytokine production in splenocytes, PCP treatment significantly increased the production of Th 1 cytokines [IL-2 and interferon (IFN)-${\gamma}$], but not Th 2 cytokines (IL-4). Therefore, PCP can induce immune cell activation and is a potential candidate for the development of nutraceuticals to boost the immune system.

Characteristics of Persimmon Juice fermented with Kimchi Lactic Acid Bacteria (김치 유산균을 이용한 감 발효음료 특성)

  • Seo, Sang Young;Ahn, Min Sil;Choi, So Ra;Song, Eun Ju;Choi, Min Kyung;Yoo, Seon Mi;Kim, Young Sun;Song, Young Ju
    • The Korean Journal of Food And Nutrition
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    • v.28 no.1
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    • pp.16-23
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    • 2015
  • This study was carried out to develop a fermented juice using persimmon (Diospyros kaki Thunb) and lactic acid bacteria isolated from kimchi, Lactobacillus buchneri BK-1, Pediococcus inopinatus BK-3 and Leuconostoc mesenteroides M-17. The total acidity value was 0.75% and viable cell number reached $1.9{\times}10^8CFU/mL$ when the persimmon and water solution was diluted by 1:3 (w/v) added with rice-syrup ($15^{\circ}Brix$) that was fermented by Lactobacillus buchneri BK-1 for 7 days. Additional levels of rice-syrup increased the total acidity of fermented juice, and the overall acceptability was the highest (4.1 point) for fermented persimmon juice added with rice-syrup $10^{\circ}Brix$. L. buchneri BK-1 and Pediococcus inopinatus BK-3 were selected to ferment the persimmon juice because there total acidity values were 0.83% and 0.80%, respectively, and the final cell concentrations, $5.1{\times}10^8$ and $2.7{\times}10^8CFU/mL$, were more than other treatment, respectively. The total acidity value of persimmon at day 3 of fermented broth were significantly higher than that of day 7 of fermented broth, and the number of viable cell declined from $8.2{\times}10^8$ to $4.3{\times}10^8CFU/mL$. In these results, the suitable period for fermentation was 4~5 days owing to the sourness being strong during fermentation.

Amplified synthesis and stability of Tn5 polypeptides in escherichia coli (대장균에서의 Tn5 단백질 증폭생합성 및 안정성)

  • 정재성;정재훈
    • Korean Journal of Microbiology
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    • v.27 no.4
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    • pp.323-333
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    • 1989
  • Plasmid DNA molecules containing strong promoter upstream from IS50L or IS50R, the two insertion sequences that flank Tn5, were constructed to amplify the synthesis of Tn5-encoded polypeptides. When proteins made by cells that contain these plasmids were analyzed on polyacrylamide gels, enhanced synthesis of IS50R polypeptides could be detected. Synthesis of this polypeptide apparently is initiated within the large open reading frame of this element. In addition, the stability of IS50L-and IS50R-encoded polypeptides was analyzed. It was found that IS50L polypeptides are relatively unstable in vivo. This instability could account for the observed inability of this element to promote transposition.

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Antioxidative and Physiological Activities of Traditional Korean Teas (한국 전통차의 생리활성 및 항산화작용)

  • Son, Jong-Youn;Kim, Tai-Ok
    • Korean journal of food and cookery science
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    • v.27 no.5
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    • pp.567-575
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    • 2011
  • This study was investigated the antioxidative and antimicrobial activities of a water extract (70$^{\circ}C$) of traditional Korean teas (rose, chrysanthemum, pine needles, mulberry, persimmon leaves, and green tea). Total phenol contents in rose, chrysanthemum, pine needles, mulberry, persimmon leaves, and green tea were 272.8, 74.6, 153.5, 73.5, 69.5, and 260.8 mg tannic acid/g extract, respectively, whereas total flavonoid contents were 75.1, 47.8, 26.8, 40.0, 27.9, and 99.5 mg quercetin/g extract, respectively. The order of electron donating abilities of Korean traditional tea water extracts (1 mg/mL) were : rose (96.8%) > green tea (95.3%) > pine needles (71.3%) > chrysanthemum (36.8%) > mulberry (28.9%) ${\qeq}$ persimmon (28.8%). The order of nitrite-scavenging abilities at pH 1.2 (2 mg/mL) was green tea > rose > pine needles > chrysanthemum ${\qeq}$ mulberry ${\qeq}$ persimmon. The order of hydroxyl radical scavenging activity (5 mg/g) was chrysanthemum > mulberry > rose > persimmon leaves > pine needles > green tea. Rose extracts showed antimicrobial activity against Micrococcus luteus, Bacillus cereus, Salmonella enteritidis, and E. coli, whereas green tea extracts showed strong antimicrobial activity aginst S. enteritidis.