• Journal of the Korean Chemical Society
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• v.61 no.1
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• pp.7-11
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• 2017

The extended $H{\ddot{u}}ckel$ method is employed to analyze the interaction of carbon monoxide with the TiC(001) surfaces, both perfect and containing carbon vacancies. CO exhibits a similar ${\sigma}$-donation interaction for both $Ti_{25}C_{25}$ and $Ti_{25}C_{23}$ clusters, as deduced from the fact that the populations of the CO $5{\sigma}$ orbital are identical upon adsorption, but it bonds more strongly with the $Ti_{25}C_{23}$ than with the $Ti_{25}C_{25}$ because the metal d electron density in $Ti_{25}C_{23}$ provides ${\pi}$ back-bonding interactions with CO that are absent in $Ti_{25}C_{25}$. This work suggests that a difference in reactivity toward CO of stoichiometric TiC and TiC with carbon defects is connected with the occupancy of $2{\pi}^*$ orbitals that leads to a significant weakening of the C-O bond.

• Molecules and Cells
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• v.28 no.1
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• pp.43-48
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• 2009

By screening C. elegans mutants for severe defects in germline proliferation, we isolated a new loss-of-function allele of cdc-25.1, bn115. bn115 and another previously identified loss-of-function allele nr2036 do not exhibit noticeable cell division defects in the somatic tissues but have reduced numbers of germ cells and are sterile, indicating that cdc-25.1 functions predominantly in the germ line during postembryonic development, and that cdc-25.1 activity is probably not required in somatic lineages during larval development. We analyzed cell division of germ cells and somatic tissues in bn115 homozygotes with germline-specific anti-PGL-1 immunofluorescence and GFP transgenes that express in intestinal cells, in distal tip cells, and in gonadal sheath cells, respectively. We also analyzed the expression pattern of cdc-25.1 with conventional and quantitative RT-PCR. In the presence of three other family members of cdc-25.1 in C. elegans, defects are observed only in the germ line but not in the somatic tissues in cdc-25.1 single mutants, and cdc-25.1 is expressed predominantly, if not exclusively, in the germ line during postembryonic stages. Our findings indicate that the function of cdc-25.1 is unique in the germ line but likely redundant with other members in the soma.

• Archives of Pharmacal Research
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• v.23 no.3
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• pp.206-210
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• 2000

A new series of phosphonate side chain analogues of 1$\alpha$,25-dihydroxyvitamin $D_3$ (1) have been synthesized. Antiproliferative activities of theses analogues (8a,b and 9a,b) using human keratinocyte cell shows that analogues which have natural A-ring show higher activity than unnatural A-ring series and almost equally active to 1 $\alpha$,25-Dihydroxyvitamin $D_3$(1) at 1 $\mu$M level.

• Journal of Animal Science and Technology
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• v.56 no.8
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• pp.33.1-33.8
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• 2014

Background: Sox 9 is a major marker of chondrocyte differentiation. When chondrocytes are cultured in vitro they progressively de-differentiate and this is associated with a decline in Sox 9 expression. The active form of vitamin D, 1, 25 $(OH)_2D_3$ has been shown to be protective of cartilage in both humans and animals. In this study equine articular chondrocytes were grown in culture and the effects of 1, 25 $(OH)_2D_3$ upon Sox 9 expression examined. The expression of the transient receptor potential vanilloid (TRPV) ion channels 5 and 6 in equine chondrocytes in vitro, we have previously shown, is inversely correlated with de-differentiation. The expression of these channels in response to 1, 25 $(OH)_2D_3$ administration was therefore also examined. Results: The active form of vitamin D (1, 25 $(OH)_2D_3$ when administered to cultured equine chondrocytes at two different concentrations significantly increased the expression of Sox 9 at both. In contrast 1, 25 $(OH)_2D_3$ had no significant effect upon the expression of either TRPV 5 or 6 at either the protein or the mRNA level. Conclusions: The increased expression of Sox 9, in equine articular chondrocytes in vitro, in response to the active form of vitamin D suggests that this compound could be utilized to inhibit the progressive de-differentiation that is normally observed in these cells. It is also supportive of previous studies indicating that $1{\alpha}$, 25-dihydroxyvitamin $D_3$ can have a protective effect upon cartilage in animals in vivo. The previously observed correlation between the degree of differentiation and the expression levels of TRPV 5/6 had suggested that these ion channels may have a direct involvement in, or be modulated by, the differentiation process in vitro. The data in the present study do not support this.

• Fire Science and Engineering
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• v.17 no.3
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• pp.1-6
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• 2003

In this study, it reviewed about evacuation performance of a specified Office Building. assessment tools is FAST 3.1.7 (Estimation of Flash Over, Estimation of Layer Height Down Flow Time), SIMULEX 32-bit (Estimation of Evacuation Time), JASMINE 3.25d. (Smoke Flow Assessment of a specified time) Result from Fire Scenario # 1, Flash Over is not generated in Compartment. Evacuation Time is estimated 25.2 sec by SIMULEX 32-bit. layer height until this time (25.2 sec) was estimated 2.4 m by FAST 3.1.7. After ignition until this time (25.2 sec), smoke was not release to the a corridor. In consequence, We concluded that people in building are completing the safe evacuation without the damage of smoke. Result from Fire Scenario # 1, Flash Over generated 6 min 33.2 sec in Compartment. Evacuation Time is estimated 1 min 25.5 sec by SIMULEX 32-bit. layer height down flow time is 1 min 40.8 sec by FAST 3.1.7 and 5 min 23 sec by theoretical calculation. Also, total building evacuation time was estimated 2 min 26.6 sec. After ignition until this time (2 min 26.6 sec), smoke released to the a corridor but it amount was few little. Therefore, generated smoke in compartment not effected to the people in buildings.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.5
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• pp.709-716
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• 2016

Recently, the operating conditions of the various mechanical structures have become more severe and the running time has become longer as the development of plant equipment increases with the introduction of high technology. Thus, the reliability of the system and its accessories is becoming a problem. Normally, synthetic natural gas (SNG) plants use 1.25Cr-0.5Mo or 2.25Cr-1Mo heat resistant steel according to the operating conditions. In this study, a lab-scale reactor was set up using 1.25Cr-0.5Mo steel, in order to carry out corrosion tests for producing synthetic natural gas. The corrosive characteristics were investigated under 1st-methanator operating conditions and fundamental data about the durability and reliability were obtained by using the experimental test. The analysis of results obtained on the durability of the reactor under emission and injection compositions showed that the hydrogen embrittlement caused by hydrogen and the oxidation corrosion caused by H2O had the most effect on the durability of 1.25Cr-0.5Mo steel in the SNG reactor. However, the hydrogen embrittlement and oxidation corrosion occurred simultaneously under emission conditions, so that the corrosion of the material increased suddenly after a long operating time. Besides, the corrosion of the 1.25Cr-0.5Mo steel under the injection composition was faster than that under the emission composition.

• Journal of Oral Medicine and Pain
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• v.34 no.3
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• pp.261-276
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• 2009

Lactacystin, a microbial natural product synthesized by Streptomyces, has been commonly used as a selective proteasome inhibitor in many studies. Proteasome inhibitors is known to be preventing the proliferation of cancer cells in vivo as well as in vitro. Furthermore, proteasome inhibitors, as single or combined with other anticancer agents, are suggested as a new class of potential anticancer agents. This study was undertaken to examine in vitro effects of cytotoxicity and growth inhibition, and the molecular mechanism underlying induction of apoptosis in SCC25 human tongue sqaumous cell carcinoma cell line treated with lactacystin. The viability of SCC25 cells, human normal keratinocytes (HaCaT cells) and human gingiva fibroblasts (HGF-1 cells), and the growth inhibition of SCC25 cells were assessed by MTT assay and clonogenic assay respectively. The hoechst staining, hemacolor staining and TUNEL staining were conducted to observe SCC25 cells undergoing apoptosis. SCC25 cells were treated with lactacystin, and Western blotting, immunocytochemistry, confocal microscopy, FAScan flow cytometry, MMP activity, and proteasome activity were performed. Lactacystin treatment of SCC25 cells resulted in a time- and does-dependent decrease of cell viability and a does-dependent inhibition of cell growth, and induced apoptotic cell death. Interestingly, lactacytin remarkably revealed cytotoxicity in SCC25 cells but not normal cells. And tested SCC25 cells showed several lines of apoptotic manifestation such as nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA contents, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, the up-regulation of Bax, and the activation of caspase-7, caspase-3, PARP, lamin A/C and DFF45 (ICAD). Flow cytometric analysis revealed that lactacystin resulted in G1 arrest in cell cycle progression which was associated with up-regulation in the protein expression of CDK inhibitors, $p21^{WAF1/CIP1}$ and $p27^{KIP1}$. We presented data indicating that lactacystin induces G1 cell cycle arrest and apoptois via proteasome, mitochondria and caspase pathway in SCC25 cells. Therefore our data provide the possibility that lactacystin could be as a novel therapeutic strategy for human tongue squamous cell carcinoma.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2006.04a
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• pp.95-105
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• 2006

Alzheimer's disease (AD) is an irreversible, progressive brain disorder that is characterized by dementia. Amounts of p25 and cdk5 kinase activity are specifically upregulated in AD patient's brain samples. Considerable evidence now points importance of p25/cdk5 in generation of A$\beta$ peptides and hyperphosphorylation of tau linking amyloid plaques and neurofibrillary tangles, two major pathological hallmarks of AD. We demonstrated that P25/CDK5 phosphorylates BACE1, the first step protease to produce A$\beta$. P25/CDK5 inhibitors to reduce BACE1 phosphorylation and the secretion of A$\beta$ are screened through in silica, in vitro, and cell-based assays. Out of 4.3 million chemicals we finally selected two compounds to have IC50 of 10 microM in cell-based assays. The inhibitors block Tau phosphrorylation as well as BACE1 phosphorylation. In conclusion P25 should be one of the best targets for AD therapeutics.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.1
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• pp.15-21
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• 2017

In the present work, the synergistic effect of seaweed addition on organic acid production from sludge was investigated. The batch experiment was conducted at various mixing ratios of sewage sludge and seaweed (100:0, 75:25, 50:50, 25:75, 0:100 on a COD basis) under the substrate concentration of 20 g COD/L. The fermentation temperature was conducted under mesophilic condition ($35^{\circ}C$) and a heat-treated ($90^{\circ}C$ for 20 min) anaerobic digester sludge was used as a seeding source to suppress the methanogenic activity, The results showed that the amount of organic acid production increased as the content of seaweed increased: organic acids were 1.45, 3.22, 4.28, 5.24 and 4.82 g COD/L for the mixing ratio of 100:0, 75:25, 50:50, 25:75 and 0:100 respectively. The synergistic effect was calculated based on the organic acid production of individual sludge and seaweed, and was found to be 0.92, 1.14, 1.26 g COD/L at the mixing ratio of 75:25, 50:50 and 25:75, which indicates that 40% of synergy was obtained when 25% of seaweed was added. The synergistic effect could be ascribed to the high C/N ratio and biodegradability of seaweed.

• The Korean Journal of Food And Nutrition
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• v.25 no.4
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• pp.855-862
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• 2012

Resveratrol (RVT) and epigallocatechin gallate (EGCG) individually inhibit adipogenesis in 3T3-L1 adipocytes. The objective was to examine the possibility of interaction between RVT and EGCG, resulting in enhanced inhibition of adipogenesis in 3T3-L1 adipocytes. Preadipocytes were treated with RVT and EGCG individually at 6.25 or $25{\mu}M$ (RVT6.25 or RVT25) and 12.5 or $50{\mu}M$ (EGCG12.5 or EGCG50) and in combination (RVT6.25 + EGCG12.5 and RVT25 + EGCG50). RVT25 as an individual compound decreased lipid accumulation in 3T3-L1 adipocytes by 24%, and RVT25 + EGCG50 further decreased lipid accumulation by 77%. In addition, exposure of 3T3-L1 adipocytes to RVT6.25 + EGCG12.5 and RVT25 + EGCG50 combinations resulted in an enhanced increase of adiponectin release and inhibition of leptin release. Quantitative analysis revealed that the combination of tested materials (RVT6.25 + EGCG12.5 and RVT25 + EGCG50) decreased the expression levels of C/EBP${\alpha}$, PPAR${\gamma}2$, and aP2. These results indicate that the combined treatments with RVT and EGCG produce synergistic effects on inhibiting adipogenesis in 3T3-L1 adipocytes. The overall results suggested that the combining RVT and EGCG might be more capable of exerting antiobesity effects than each individual compound by itself.