• Experimental and Molecular Medicine
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• 제50권11호
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• pp.11.1-11.12
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• 2018

USP15 has been shown to stabilize transcription factors, to be amplified in many cancers and to mediate cancer cell survival. However, the underlying mechanism by which USP15 regulates multiple myeloma (MM) cell proliferation and apoptosis has not been established. Here, our results showed that USP15 mRNA expression was upregulated in MM patients. USP15 silencing induced MM cell proliferation inhibition, apoptosis, and the expression of nuclear and cytoplasmic NF-${\kappa}Bp65$, while USP15 overexpression exhibited an inverse effect. Moreover, in vivo experiments indicated that USP15 silencing inhibited MM tumor growth and NF-${\kappa}Bp65$ expression. PDTC treatment significantly inhibited USP15 overexpression-induced cell proliferation, apoptosis inhibition, and NF-${\kappa}Bp65$ expression. USP15 overexpression promoted NF-${\kappa}Bp65$ expression through inhibition of its ubiquitination, whereas NF-${\kappa}Bp65$ promoted USP15 expression as a positive regulator. Taken together, the USP15-NF-${\kappa}Bp65$ loop is involved in MM tumorigenesis and may be a potential therapeutic target for MM.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.535-547
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• 2023

Sea cucumbers (Holothuria scabra), also known as beche-de-mer, are highly valued as a luxurious food item and have been utilized as a traditional tonic food in various Asian countries for centuries. The body walls of sea cucumbers are the main edible part, which are primarily composed of glycosaminoglycan (GAG). The rehydration of dried sea cucumber is a crucial step prior to further processing. The aim of this study was to assess the impact of ultrasound-assisted rehydration (UAR) on the quality of dried sea cucumbers. The experiment used four different rehydration methods, including conventional methods at 27℃ (KV27℃) and 15℃ (KV15℃), as well as a combination of ultrasound at 27℃ with conventional at 15℃ (UAR27 + KV15℃) and ultrasound at 15℃ with conventional at 15℃ (UAR15 + KV15℃). Results indicated that the rehydration rate (RR) was significantly affected by both the rehydration method and the temperature used (p < 0.05). UAR27 + KV15℃ was identified as the most effective method in terms of rehydration behavior and quality characteristics of dried sea cucumber, with a RR of 0.58 ± 0.53 gH₂O/hour and reduced rehydration time of up to 28 hours. Moreover, the UAR27 + KV15℃ method demonstrated superior rehydration potential, nutritional value (proximate composition and sulfate content), color, lower energy, and microstructure properties compared to the other methods. The sulfate content and yield of sulfated GAGs were determined to be 89.4 mg/g and 52.8 ㎍/g, respectively. Confirmation of the absorption band of the sulfate group showed the presence of 3-N-acetyl galactosamine at a wavelength of 1,269 cm^-1 and C-O-S at 860 cm^-1. The sea cucumbers treated with UAR exhibited a GAG content approximately 2.9 times higher than those rehydrated with the conventional method. Eventually, the combination of UAR at 27℃ with conventional at 15℃ methods can significantly accelerate the rehydration of sea cucumber without negatively affecting its physical quality properties.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 추계학술대회
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• pp.67-68
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• 2013

본 논문에서는 1-15GHz 주파수 대역에서 동작하는 초광대역 인쇄형 다이폴 안테나에 대한 설계방법을 연구하였다. 초광대역 특성을 얻기 위하여 반원 모양의 다이폴 소자를 이용하여 두 팔 사이의 간격과 반원의 반지름 변화에 따른 입력 반사계수와 이득 특성을 분석하여 최적의 설계 변수를 도출하였다. 최적화된 인쇄형 반원모양 다이폴 안테나를 FR4 기판 상에 $100mm{\times}100mm$ 크기로 제작하였다. 실험 결과, 전압 정재파비(VSWR; voltage standing wave) < 2인 대역이 1-15 GHz (대역폭 비 15:1, 175%)으로 초광대역 특성을 갖는 것을 확인하였다.

• 한국항행학회논문지
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• 제15권5호
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• pp.764-773
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• 2011

IEEE 802.15.4는 TDMA(time division multiple access) 방식으로 동작하는 LWPAN(low-rate wireless personal area networks)의 표준이다. 그러나 IEEE 802.15.4는 QoS(quality of service), 확장성, 신뢰성 등에 제약이 있다. 기존 IEEE 802.15.4에서는 QoS 지원을 위하여 GTS(guaranteed time slot)을 사용한다. 그러나 GTS는 멀티 홉 환경에서 다수의 장치들의 QoS를 만족시키기에 효과적인 방법이 아니며, 멀티 주파수 채널에 대한 고려도 없다. 이러한 제약 사항을 보완하기 위하여 IEEE 802.15.4의 MAC(medium access control) 부계층을 강화한 표준으로써 IEEE 802.15.4e가 개발 중이다. 본 논문에서는 IEEE 802.15.4e에서 멀티 채널, 멀티 홉 환경의 종단 장치간 전송 지연을 만족시키기 위한 스케줄링 방식을 제안하고, 시뮬레이터를 이용하여 성능 평가를 수행하였다.

• 조명전기설비학회논문지
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• 제17권6호
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• pp.169-172
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• 2003

입력 AC 120[V]-15[A]로 제한된 미국의 전원콘센트에서 출력 DC 15[V]-100[A]를 얻을 수 있도록 SCR 위상제어방식을 이용한 자동차 오디오 장착용 정전압회로를 설계하였다. 제시된 설계에 따라 자동차용 전원공급장치를 실험한 결과 리플전압이 110[mV]인 출력 DC 15[V]-100[A]를 얻었다.

• Journal of Microbiology
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• 제42권3호
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• pp.188-193
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• 2004

Xanthobacter flavus strain UE15 was isolated in wastewater obtained from the Ulsan industrial complex, Korea. This strain functions as a 1,2-dichloroethane (1,2-DCA) degrader, via a mechanism of hydrolytic dechlorination, under aerobic conditions. The UE15 strain was also capable of dechlorinating other chloroaliphatics such as 2-chloroacetic acid and 2-chloropropionic acid. The dhlA gene encoding 1,2-DCA dechlorinase was cloned from the genomic DNA of the UE15 strain, and its nucleotide sequence was determined to consist of 933 base pairs. The deduced amino acid sequence of the DhlA dechlorinase exhibited 100％ homology with the corresponding enzyme from X. autotrophicus GJ10, but only 27 to 29％ homology with the corresponding enzymes from Rhodococcus rhodochrous, Pseudomonas pavonaceae, and Mycobacterium sp. strain GP1, which all dechlorinate haloalkane compounds. The UE15 strain has an ORF1 (1,356 bp) downstream from the dhlA gene. The OFR1 shows 99％ amino acid sequence homology with the transposase reported from X. autotrophicus GJ10. The transposase gene was not found in the vicinity of the dhlA in the GJ10 strain, but rather beside the dhlB gene coding for haloacid dechlorinase. The dhlA and dhlB genes were confirmed to be located at separate chromosomal loci in the Xanthobacter flavus UE15 strain as well as in X. autotrophicus GJ10. The dhlA and transposase the UE15 strain were found to be parenthesized by a pair of insertion sequences, 181247, which were also found on both sides of the transposase gene in the GJ10 strain. This unique structure of the dhlA gene organization in X. flavus strain UE15 suggested that the dechlorinase gene, dhlA, is transferred with the help of the transposase gene.

• 한국초지조사료학회지
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• 제35권1호
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• pp.50-56
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• 2015

이탈리안 라이그라스(Lolium multiflorum Lam., IRG)는 주로 답리작으로 재배되고 있으며 파종기는 벼 수확시기와 겹치고 파종기에 강우에 의하여 파종 적기를 놓칠 수 있다. 따라서 본 연구는 이탈리안 라이그라스의 적기 파종을 놓쳤을 때 봄 파종시 수량성 및 사료가치에 미치는 영향을 검토하고자 2012월 10월부터 2013년 6월까지 전라남도 강진에서 수행하였다. 파종시기를 주구로 2월 15일, 2월 25일, 3월 5일, 3월 15일로 4처리 하였으며, 품종을 세 구로 극조생종 그린팜(Greenfarm), 조생종 코윈어리(Kowinearly) 그리고 중생종 코윈마스터(Kiwinmaster)를 공시하였다. 이탈리안 라이그라스의 파종시기별 출수기는 2월 15일 파종에서는 Kowinmaster, Kowinearly 및 Greenfarm은 각각 5월 14일, 5월 12일, 5월 9일로 극조생종과 중생종간의 차이는 5일의 숙기 차이가 있었으나, 3월 15일 파종에서는 각각 5월 23일, 5월 22일, 5월 20일로 극조생종과 조생종간의 생육차이는 3일의 숙기 차이가 나타났다. 그리고 초장은 Kowinmaster를 2월 15일 파종하였을 때 105.7 cm로 3월 15일에 파종하였을 93.0 cm 보다 길었으며, Kowinearly와 Greenfarm 품종도 2월 15일과 2월 25일 파종구에서 초장은 비슷하였으나, 3월 5일과 3월 15일에서는 짧았다. 그리고 도복은 빨리 파종하였을 때 심했으며, 품종간의 차이는 적었다. 그리고 건물수량은 Kowinearly 조생종을 2월 15일에 파종하여 5월 30일에 수확할 때 7,333 kg/ha으로 가장 많았고, Green farm 극조생종에서 6,609 kg/ha으로 적었으며, 유의적 차이는 없었다. 그러나 파종시기별 건물수량은 2월 15일에 파종했을 때 7,052 kg/ha으로 3월 15일 파종구의 4,698 kg/ha 보다 유의적으로 많았다(P<0.05). 조단백질의 경우 2월 15일 파종에서 평균 8.8%인데 반해 3월 15일 파종에서 평균 11.1%로 약 2.3% 높게 나타났으며, ADF는 2.1% 낮게 나타났고 TDN도 1.5% 정도 높았다. 그리고 RFV에서는 5.9% 정도 높아 파종시기가 늦어짐에 따라 사료가치는 좋았으며 품종간의 차이는 적었다. 이상의 결과를 종합적으로 고려할 때 남부지역 논에서 이탈리안 라이그라스의 봄 파종 적기는 2월 25일경이며, 최소한 2월말까지는 파종해야 수량성과 사료가치가 높은 조사료(forage)를 생산할 수 있을 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제32권11호
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• pp.3861-3864
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• 2011

Rhodamine B dye (RB) has been introduced into the mesoporous silica (SBA15) and Ag anchored mesoporous silica by applying solution impregnation method. Surface treatment of SBA15 with 3-aminopropyltrimethoxysilane (APTMS) facilitates selective anchoring of the RB molecules on SBA15. The photoluminescence spectra of RB confined within SBA15 indicates higher emission intensity, than that of the RB solid, particularly in the presence of Ag nanoparticles. The significant enhancement in photoluminescence intensity is attributed to the local enhancement of the optical fields near the molecules by interactions with silver plasmons.

• 대한임베디드공학회논문지
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• 제4권1호
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• pp.16-22
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• 2009

This paper aims at designing an impulse-radio ultra-wideband (IR-UWB) transceiver, especially targeting the IEEE 802.15.4a indoor ranging and communication systems. We first investigate the IEEE 802.15.4a IR-UWB signals and suggest the full-digital transceiver architecture accordingly. Since the wireless systems equipped with the impulse signal have the property of low-duty cycle, i.e., discontinuity in time, while the conventional systems takes the continuous signals, it is required to reconfigure the system design, including link budget. Following brief introduction to our IEEE 802.15.4a IR-UWB system hardware, we finally examine the ranging performance in indoor environments to verify our system design.

• 약학회지
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• 제60권1호
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• pp.1-7
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• 2016

The aim of this study was to investigate the prevalence of quinolone resistant E. coli from retail meat and to characterize the resistant determinants. Determination of minimum inhibitory concentration, the sequence analysis of gyrA, gyrB, parC, and parE quinolone resistance determining regions (QRDR), the presences of plasmid mediated quinolone resistance (PMQR) and the expression of efflux pump genes were investigated. Of the total 277 retail meat samples, 67 coli form bacteria were isolated. 15 of 67 isolates showed nalidixic acid resistance and 7 of 15 nalidixic acid resistant isolates were also resistant to ciprofloxacin, moxifloxacin and levofloxacin. 11 of 15 nalidixic acid resistant strains were isolated from chicken, 2 of 15 were isolated from beef and 2 of 15 were isolated from pork samples. 11 of 15 nalidixic acid resistant strains have single mutation at codon 87 (D87N or D87G) in gyrA, 2 of 11 gyrA mutants have double mutations at codon 86 and 87 (L86A and L87I) in parC with mutations at codon 434+445+465 or 429 in gyrB. 2 of 15 resistant isolates harbored qnrS, a PMQR determinant. Over expression of the acrB gene, efflux pump gene (3.93~16.53 fold), was observed in 10 of 15 resistant isolates.