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http://dx.doi.org/10.17480/psk.2016.60.1.1

Molecular Characterization of Quinolone Antibiotic Resistance in Escherichia coli Isolated from Retail Meat in Seoul  

Park, Ji Min (Department of Animal Biotechnology, Sahmyook University)
Choi, Sung Sook (College of Pharmacy, Sahmyook University)
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YAKHAK HOEJI / v.60, no.1, 2016 , pp. 1-7 More about this Journal
Abstract
The aim of this study was to investigate the prevalence of quinolone resistant E. coli from retail meat and to characterize the resistant determinants. Determination of minimum inhibitory concentration, the sequence analysis of gyrA, gyrB, parC, and parE quinolone resistance determining regions (QRDR), the presences of plasmid mediated quinolone resistance (PMQR) and the expression of efflux pump genes were investigated. Of the total 277 retail meat samples, 67 coli form bacteria were isolated. 15 of 67 isolates showed nalidixic acid resistance and 7 of 15 nalidixic acid resistant isolates were also resistant to ciprofloxacin, moxifloxacin and levofloxacin. 11 of 15 nalidixic acid resistant strains were isolated from chicken, 2 of 15 were isolated from beef and 2 of 15 were isolated from pork samples. 11 of 15 nalidixic acid resistant strains have single mutation at codon 87 (D87N or D87G) in gyrA, 2 of 11 gyrA mutants have double mutations at codon 86 and 87 (L86A and L87I) in parC with mutations at codon 434+445+465 or 429 in gyrB. 2 of 15 resistant isolates harbored qnrS, a PMQR determinant. Over expression of the acrB gene, efflux pump gene (3.93~16.53 fold), was observed in 10 of 15 resistant isolates.
Keywords
quinolone resistant; E. coli; retail meat; QRDR; PMQR; acrB;
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