• Asian Pacific Journal of Cancer Prevention
• /
• 제14권10호
• /
• pp.5935-5939
• /
• 2013

Our study is to determine the presence of endometrial intraepithelial neoplasia (EIN) in endometrial biopsy specimens classified by the 1994 World Health Organization (WHO) criteria for endometrial hyperplasia. Endometrial biopsy specimens that were stained with hematoxylin and eosin (HE) were examined and categorized by the WHO 1994 criteria and for the presence of EIN as defined by the International Endometrial Collaborative Group. ${\beta}$-catenin expression was examined by immunohistochemistry. A total of 474 cases of HE stained endometrial biopsy tissues were reviewed. There were 379 cases of simple endometrial hyperplasia, 16 with simple atypical endometrial hyperplasia, 48 with complex endometrial hyperplasia, and 31 with complex atypical endometrial hyperplasia. Among the 474 endometrial hyperplasia cases, there were 46 (9.7%) that were classified as EIN. Of these 46 cases, 11(2.9%) were classified as simple endometrial hyperplasia, 1 (6.3%) as simple atypical endometrial hyperplasia, 6 (12.5%) as complex endometrial hyperplasia, and 28 (90.3%) as complex atypical endometrial hyperplasia. EIN was associated with a higher rate of ${\beta}$-catenin positivity than endometrium classified as benign hyperplasia (72% vs. 22.5%, respectively, P<0.001), but a lower rate than endometrial adenocarcinoma (72% vs. 96.2%, respectively, P<0.001). In benign endometrial hyperplasia, high ${\beta}$-catenin expression was noted in the cell membranes, whereas in EIN and endometrial adenocarcinoma high expression was noted in the cytoplasm. In conclusion, EIN is more accurate than the WHO classification for the diagnosis of precancerous lesions of the endometrium.

• 한국미생물·생명공학회지
• /
• 제18권3호
• /
• pp.273-279
• /
• 1990

PHB 생산을 위하여 메탄올을 기질로 한 선별배지에서 토양, 하천수, 퇴비 등으로부터 분홍색 색소를 가지는 PHB 축적 facultative methylotroph를 분리하여, 균주의 특성을 검토하였다. 분리균주의 최적 생육조건과 PHB 축적을 위한 배양조건을 조사한 결과 균체의 생육은 메탄올 농도 1.0(v/v), 질소원인$ NH_4C$ 농도 1.0g/l, 즉 C/N ratio 13.2 일때 그리고 pH 7.0과'$30^{\circ}C$에서 가장 좋았으며, PHB는 C/N ratio가 50.8, 즉 메탄올 농도 1.0(v/v )$NH_4CL$ 0.26g/l 일때, 그리고 pH 6.0일 때 건조중량의 약 40까지 축적되었다. 고농도 메탄올에 의한 생육저해를 극복하기 위하여 기질을 간헐적으로 계속 공급해주는 fed-batch 배양을 시도한 결과 균체량은 14g/l, PHB 축척량은 5.5g/l까지 증가시킬 수 있었다. 생산된 PHB를 분리.정제하여 IR과 $^I H-NMR$로 구조를 분석한 결과 3-hydroxybutyric acid 의 homopolymer임이 확인되었다. 또한 균주의 pink-pigment를 추출하여 absorption spectrum를 조사하여 그 특성을 규명하였다.

• 대한통합의학회지
• /
• 제5권3호
• /
• pp.91-99
• /
• 2017

Purpose: The purpose of this study was to examine the relationship among self-efficacy, career attitude maturity, and campus life satisfaction in nursing college students. Method: A total of 277 students agreed to participate in this study from 1 May 2016 to 31 May 2016. Data analysis included t-test, ANOVA, Scheffe's test, and multiple regression using the SPSS/WIN 18.0 program. Results: The results of the analysis revealed a positive correlation between campus life satisfaction and career attitude maturity (r=.316, p=.001); between campus life satisfaction and self-efficacy (r=.256, p=.001); and between self-efficacy and career attitude maturity (r=.469, p=.001). Career attitude maturity had the highest R-squared value of 10% (${\beta}=.22$) for campus life satisfaction, while peer relationships had an R-squared value of 2% (${\beta}=-.18$), residence type of 2% (${\beta}=.14$), and self-efficacy of 1% (${\beta}=.14$), for a total R-squared value of 15%. Discussion: Given these results, individual counseling is recommended to improve campus life satisfaction by helping college students to acquire the skills to foster good interpersonal relationships, self-efficacy, and a positive view of their future vocation. Furthermore, it is essential for an educational environment to support students to ensure that after graduation they become fully-fledged members of society with a sense of pride in their profession.

• 디지털융복합연구
• /
• 제14권3호
• /
• pp.391-399
• /
• 2016

본 연구는 간호대학생의 임상실습 시 폭력경험, 대처양식 및 회복탄력성이 정서반응과 임상실습스트레스에 미치는 효과를 알아보고자 실시하였다. 대상자는 간호대학생 261명이 참여하였고 2014년 12월 1일부터 12월 20일까지 자가보고식 질문지를 사용하여 조사하였다. 상관관계 분석 결과 폭력경험은 정서중심대처, 임상실습스트레스와 유의한 정적 상관관계가 있었으며, 문제중심대처는 정서중심대처, 회복탄력성과 유의한 정적 상관관계가 있었다. 정서 중심대처는 정서반응, 임상실습스트레스와 유의한 정적 상관관계가 있었으며, 회복탄력성은 정서반응과 유의한 부적상관관계가 있었고, 정서반응은 임상실습스트레스와 유의한 정적 상관관계가 있었다. 정서반응에 영향을 미치는 가장 높은 영향요인은 정서중심대처(${\beta}=.422$)였다. 임상실습스트레스에 영향을 미치는 가장 높은 영향요인은 폭력경험(${\beta}=.283$)이였다. 간호대학생의 안전한 실습환경을 위해 대인관계훈련 프로그램과 효율적인 의사소통훈련 프로그램을 개발하는 것이 필요할 것으로 제언한다.

• 대한화학회지
• /
• 제46권4호
• /
• pp.330-336
• /
• 2002

새로운 ${\alpha},{\beta}$-불포화Carboxanilide계열의 살균제를 개발을 목적으로 4-acetyl-3-methyl-N-phenyl-1,4-thiazine-2-Carboxamide(6)의 합성에 대하여 기술하였다. Dihydro-1,4-thiazine methyl ester 11을 산화하여 생성된 sulfoxide 7을 Pummerer 반응하여 $\alpha$-acetoxy dihydro-1,4-thiazine 10a를 얻었다. 반며에 Dihydro-1,4-thiazine Carboxanilide sulfoxide14는 동일한 반응조건 하에서 중간체 sulfonium ion의 carboxanilide기가 관여한 vinylogous Pummerer 반응의 전위에 의하여 중간체를 15를 통하여 acetoxymethyl dihydro-1,4-thiazine 18이 생성되었다. 1,4-Thiazine carboxanilide 6은 $\alpha$-acetoxy dihydro-1,4-thiazine 10a를 산촉매로 처리한 다음 생성된 21a를 가수분해하고 아닐린과 결합하여 합성하였다

• Journal of Yeungnam Medical Science
• /
• 제28권2호
• /
• pp.153-164
• /
• 2011

Background: It has been reported that estrogen receptor beta ($ER{\beta}$) mRNA expression was down-regulated during carcinogenesis and was inversely related to estrogen receptor alpha ($ER{\alpha}$) expression in breast cancer. The association of $ER{\beta}$ mRNA expression to tamoxifen resistance has also been reported. In this study, the expression of $ER{\alpha}$ and $ER{\beta}$ via immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) was prompted, and an attempt was made to find out the relationship between $ER{\beta}$ expression and recurrence in the hormonal therapy group, and between $ER{\beta}$ expression and known prognostic factors. Methods: Tumor specimens were obtained at surgery from 67 female breast cancer patients during the period of September 1995 to December 2000. All the specimens were frozen in liquid nitrogen and kept at $-70^{\circ}C$ until they were used. The medical records were analyzed retrospectively. The expressions of ER were analyzed using IHC and RT-PCR methods. Results: The median follow-up was at 93.0 months (range: 14-157 months). The percentage of $ER{\alpha}+/ER{\beta}+$, $ER{\alpha}+/ER{\beta}-$, $ER{\alpha}-/ER{\beta}+$, and $ER{\alpha}-/ER{\beta}$ group were 35.9% 9.4%, 47.2%, and 7.5%, respectively, in 53 patients with hormonal therapy. $ER{\beta}$ was positive in 42 (82.3%) of 51 ER-positive patients. In the hormonal therapy group, the recurrence rates of each group was 15.8%, 0%, 40.0%, and 0%, respectively. In this group, the $ER{\beta}$ expression tended to recur, but there was no clinical significance (p=0.084). Conclusion: The $ER{\beta}$ expression may be a predictive marker of a poor response to endocrine therapy in breast cancer patients, although this needs to be confirmed in additional studies.

• 대한금속재료학회지
• /
• 제49권2호
• /
• pp.121-127
• /
• 2011

The mechanical properties of the various heat treatment conditions on Ti-15Mo-3Nb-3Al-0.2Si alloy plates were examined. XRD patterns from the surface of Ti-15Mo-3Nb-3Al-0.2Si were analyzed as a solution-treated Ti alloy has the single-phase ${\beta}$ structure whereas the aged Ti alloys have the ${\beta}$ matrix embedded with ${\alpha}$ needles. High strength (~1500 MPa) with decent ductility (7%) was obtained by the Ti alloy double aged at $300^{\circ}C$ and $520^{\circ}C$ for 8 hours each. The double-aged alloy exhibits the finer structure than the single-aged alloy at $300^{\circ}C$ for 8 hours because of the higher nucleation rate of ${\alpha}$ needles at an initial low aging temperature ($320^{\circ}C$). TEM observation revealed that the fine nanostructure with ${\alpha}$ needles in the ${\beta}$ matrix ensured the excellent mechanical properties in the double aged Ti-15Mo-3Nb-3Al-0.2Si alloy. In the solution treated alloy, the yield drop, stress-serrations and the ductility minimum typically associated with dynamic strain aging can be attributed to the dynamic interaction between dislocations and oxygen atoms. The yield drop and the stress serration were not observed in aged samples because the geometrically introduced dislocations due to phase precipitates suppressed the dynamic strain aging.

• 한국수산과학회지
• /
• 제31권2호
• /
• pp.278-287
• /
• 1998

담수산 어류에 관한 비교 생화학적 연구의 일환으로서, 우리나라 특산의 냉수성 담수 어류인 천연산 열목어와 천연산 및 양식산 산천어의 표피 carotenoids를 TLC, column chromatography 및 HPLC로 분리하고 $NaBH_4$에 의한 환원반응, $I_2$에 의한 이성화반응 및 uv-visible spect-rophotometer로 동정하여 비교, 검토한 결과는 다음과 같다. 열목어 표피의 총 carotenoids 함량은 천연산이 $3.72\;mg\%$으로서 연어과의 다른 어종에 비해 함량이 높았으며 carotenoids 조성은 zeaxanthin $36.9\%$, $\beta-carotene\;14.7\%$로서 주성분을 이루고, 그 외 triol $7.8\%$, isocryptoxan-thin $7.3\%$, 4-hydroxy echinenone $5.7\%$, lutein $4.7\%$, salmoxanthin $4.5\%$ 및 astaxathin $2.2\%$의 순으로 나타났으며, canthaxanthin, tunaxanthin A, tunaxanthin B, tunaxanthin C, $\beta-cryptoxanthin$ 및 $\alpha-cryptoxanthin$이 미량성분으로 함유되어있다. 산천어 표피의 총 carotenoids 함량은 천연산 $0.82\;mg\%$인데 비하여 양식산은 $0.66\;mg\%$ 로서 천연산이 양식산에 비해 함량이 높았다. carotenoid 조성은 천연산에서 zeaxanthin $20.7\%$, isocryptoxanthin $17.0\%\;\beta-carotene$이 $15.8\%$로서 주성분을 이루고, 그 외 $\beta-cryptoxanthin\;6.7\%$, triol $6.2\%$, 4-hydroxy echinenone $6.1\%$, salmoxanthin $6.1\%$, canthaxanthin $5.9\%$, Butein $5.8\%$, $\alpha-cryptoxanthin\;4.9\%$ 및 astaxanthin $1.0\%$의 순으로 함유하며, 양식 산에 는 isocryptoxanthin $19.7\%$, $\beta-carotene\;18.0\%$, Beaxanthin $10.3\%$이 주성분을 이루며, 그 외 $\beta-cryptoxanthin\;8.9\%$, $\alpha-cryptoxanthin\;8.5\%$, lutein $8.0\%$, canthaxanthin $7.6\%$, triol $5.1\%$ 및 astaxanthin $2.0\%$의 순으로 함유하였다. 한편 천연산 산천어에는 zeaxanthin, salmoxanrhin 및 4-hydroxy echinenone의 함량이 높은 반면 양식산 산천어에는 $\alpha$-cryptoxanthin의 함량이 높아 서로 다른 경향을 보여 상이하였다. 양식산 산천어에는 천연산 산천어와는 달리, 연어과 특유의 carotenoid인 4-hydroxy echinenone과 salmoxanthin이 검출되지 않았고 또한 천연산 및 양식산 산천어는 천연산 열목어와는 달리 tunaxanthin A, tunaxanthin B 및 tunaxanthin C가 존재하지 않았다.

• 한국산학기술학회논문지
• /
• 제17권6호
• /
• pp.598-608
• /
• 2016

본 논문은 임상간호사의 환자안전역량 강화를 위한 기초자료로 제공하기 위해 환자안전역량에 영향을 미치는 요인을 파악하고자 비판적 사고성향, 문제해결능력 및 자기효능감을 독립변수로 정하여 서술적 조사연구를 시행하였으며, 중소병원에 근무하는 6개월 이상 경력의 임상간호사 373명을 대상으로 하였다. 자료분석 결과 환자안전역량에 영향을 미치는 요인은 문제해결능력(${\beta}=.403$, p<.001)이 가장 큰 것으로 나타났으며 자기효능감(${\beta}=.156$, p=.005), 환자안전사고 보고 경험(${\beta}=.137$, p=.002), 비판적 사고성향(${\beta}=.130$, p=.018) 순으로 영향을 미치는 것으로 나타났다. 이들 변수는 대상자의 환자안전역량에 대해 42.3%(adj $R^2=.423$) 설명하였다(F=20.305, p<.001). 비판적 사고성향, 문제해결능력, 자기효능감은 학부과정에서부터 강조되는 것으로 실제 의료현장에서 환자의 안전을 최일선에서 다루는 임상간호사에게는 더욱이 필요한 요인이라고 볼 수 있다. 따라서 환자안전역량 강화를 위해서는 의료기관 자체 내에서 임상간호사를 위한 비판적 사고성향, 문제해결능력 및 자기효능감을 높이기 위한 교육프로그램과 같은 다양한 전략과 활동들이 요구된다고 생각된다. 또한 환자안전을 최우선으로 생각하고 환자안전사고 발생 시 적극적인 보고를 독려하는 한편 환자안전사고 보고를 저해하는 요인들을 면밀히 사정하여 보완하여 보고율 향상과 더불어 환자안전역량 강화에도 힘써야 할 것이다.

• Biomolecules & Therapeutics
• /
• 제3권4호
• /
• pp.266-272
• /
• 1995

The analysis of membrane receptors for hormones and neurotransmitters has progressed considerably by pharmacological and biochemical means and more recently through the use of specific antibodies. To generate and characterize a moloclonal antibody against $\beta$-adrenergic receptor, a synthetic $\beta$2-adrenergic receptor peptide (Phe-Gly-Asn-Phe-Trp-Cys-Phe-Trp-Thr-Ser-lle-Asp-Val-Leu) which may comprise part of $\beta$-adrenergic receptor ligand binding pocket was coupled to Keyhole Limpet Hemocyanin (KLH) and used as an immunogen. Male BALB/C mice were immunized with this antigen and the immunized spleen was fused with myeloma SP2/0-Ag14 cells to produce monoclonal antibodies. Two clones were obtained but one of monoclonal antibodies, mAb5G09, was used throughout in this study because the other clone, mAb5All showed weak immunoreactivity against KLH as well. The mouse monoclonal antibody mAb5G09 produced in this study showed immunoreactivity to peptide-KLH conjugates and also to human A43l cells and guinea pig lung $\beta$2-adrenergic receptor as revealed by ELISA and western blot. In the course of determination of the effects of mAb5G09 on $\beta$-receptor ligand binding, it was observed that mAb5G09 specifically bound $\beta$-adrenergic radioligand [$^3$H]dihydroalprenolol (DHA) with a dissociation constant (Kd) of 60 nM. The [$^3$H]DHA binding activity of mAb5G09 had characteristics of immunoglobulins and the binding activity was not observed in the control anti-KLH monoclonal antibody. The monoclonal antibody, mAb5G09 produced in this study may provide useful models for the study of the structure of receptor binding sites.