• Nutrition Research and Practice
• /
• 제10권3호
• /
• pp.274-281
• /
• 2016

BACKGROUND/OBJECTIVES: The accumulation of amyloid-${\beta}$ ($A{\beta}$) in the brain is a hallmark of Alzheimer's disease (AD) and plays a key role in cognitive dysfunction. Perilla frutescens var. japonica extract (PFE) and its major compound, rosmarinic acid (RA), have shown antioxidant and anti-inflammatory activities. We investigated whether administration of PFE and RA contributes to cognitive improvement in an $A{\beta}_{25-35}$-injected mouse model. MATERIALS/METHODS: Male ICR mice were intracerebroventricularly injected with aggregated $A{\beta}_{25-35}$ to induce AD. $A{\beta}_{25-35}$-injected mice were fed PFE (50 mg/kg/day) or RA (0.25 mg/kg/day) for 14 days and examined for learning and memory ability through the T-maze, object recognition, and Morris water maze test. RESULTS: Our present study demonstrated that PFE and RA administration significantly enhanced cognition function and object discrimination, which were impaired by $A{\beta}_{25-35}$, in the T-maze and object recognition tests, respectively. In addition, oral administration of PFE and RA decreased the time to reach the platform and increased the number of crossings over the removed platform when compared with the $A{\beta}_{25-35}$-induced control group in the Morris water maze test. Furthermore, PFE and RA significantly decreased the levels of nitric oxide (NO) and malondialdehyde (MDA) in the brain, kidney, and liver. In particular, PFE markedly attenuated oxidative stress by inhibiting production of NO and MDA in the $A{\beta}_{25-35}$-injected mouse brain. CONCLUSIONS: These results suggest that PFE and its active compound RA have beneficial effects on cognitive improvement and may help prevent AD induced by $A{\beta}$.

• 한국산학기술학회논문지
• /
• 제22권1호
• /
• pp.585-594
• /
• 2021

본 연구는 경전 이해를 기반으로 하는 불교와 마음치유 강의에 참여한 수강생들의 스트레스가 강의를 통해 완화됨으로써 심리적 안정과 사고능력을 증진시키고, 이해력을 높이는 효과를 확인하고자 실시한 유사 실험연구이다. 스트레스는 일반적으로 자기보고식 검사를 통해 확인할 수 있으나, 본 연구에서는 보다 객관성을 확보하기 위하여 정량뇌파를 측정하여 스트레스 수준을 평가하였다. 이를 위해 서울특별시 G구에 소재한 S대학교에서 2019년 3월부터 6월까지 진행된 불교와 마음치유 강의를 수강하는 실험군과 수강하지 않는 대조군을 7명씩 표집하여 1주 차 수업 전의 사전과 15주차 수업 후의 사후 정량뇌파 차이를 Mann Whitney U검정과 Wilcoxon 부호순위검정을 통하여 검증하였다. 그 결과, 불교와 마음치유 수업을 수강하는 실험군은 대조군에 비해 Beta파, High Beta파가 높게 나타나 대조군보다 스트레스 혹은 긴장 상태에 있는 것으로 나타났다. 실험군은 Beta파의 F7, T3, T4, T5와 High Beta파의 F7, F8, T3, T4에서 유의한 차이가 발생하였고, Coherence도 좌반구 Fp1-T5, F3-P3, F3-O1, C3-O1, P3-F7, O1-F7에서 개선된 반면, 대조군은 유의한 차이가 없었다. 따라서 불교와 마음치유 강의는 스트레스 개선 효과가 있는 것으로 나타났다.

• Natural Product Sciences
• /
• 제14권2호
• /
• pp.90-94
• /
• 2008

Two sesquiterpenes (1 - 2), a tetralone (3), and two phenylpropanoids (4 - 5) were isolated from the stem bark of Magnolia obovata Thunberg (Magnoliaceae) through repeated column chromatography. Their structures were identified as ${\beta}-eudesmol$ (1), cryptomeridiol (2), 4R-4,8-dihydroxy-${\beta}-tetralone$ (3), trans-pcoumaryl aldehyde (4), and p-coumaric acid (5) on the basis of spectroscopic analysis including two dimensional NMR and mass. Compounds 1 - 3 were tested in vitro for their cytotoxic activity against the K562, HeLa, A549, and HCT116 cancer cell lines. However, compounds 1 - 3 were inactive in this assay system.

• Archives of Pharmacal Research
• /
• 제14권3호
• /
• pp.225-230
• /
• 1991

From the roots of Rubus parvifolius L., four triterpenoidal sapogenins, ursolic acid 1. $2\alpha$-hydroxyursolic acid 2. causapic acid 3. $2\alpha$, $3\beta$, $19\alpha$-trihydroxyurs-12-en-23.28-dioic acid 4 and one triterpenoidal glycoside. suavissimoside $R_1$ 5. were isolated. The structures were elucidated by spectroscopic methods and chemical transformations. Compound 4 was first isolated as free form.

• 한국버섯학회지
• /
• 제17권2호
• /
• pp.47-51
• /
• 2019

Our research on juvenile Oncorhynchus masou masou in oyster mushroom supplemented diet was studied to investigate the effect of feeding. Mixing of feed ingredients for dried oyster mushrooms, 3.5, 7.0, 10.5, 14.0 (%) was added to the amount of oyster mushroom dietary beta-glucan content of the more abundant. After the weight of feed given to salmon survey oyster mushrooms diet for 3.5 to 7.0% was similar to the formula feed and the weight of the fish ate oyster mushroom feed over 10.5% were reduced. The oyster mushroom of the experimental diets containing 3.5 to 7.0 percent hepatosomatic index and feed coefficient figure was similar to those of the formulated diets. Therefore we have juvenile cherry salmon fed diets containing 3.5 to 7.0% was considered good to eat and additional research on the immune response will be carried out was necessary.

• Tuberculosis and Respiratory Diseases
• /
• 제50권2호
• /
• pp.182-195
• /
• 2001

연구배경 : IPF에 의한 유병률과 사망률은 점차 증가하는 추세이나 좋은 치료는 없는 상태이다. 폐 섬유화 과정에서 TGF-${\beta}_1$, TNF-$\alpha$, ET-1, IFN-$\gamma$등의 사이토카인이 중요한 역할을 함이 알려져 있다. 본 실험은 파라콰트를 기관지 내로 주입하여 섬유화가 유발되는 과정의 백서의 폐 조직 내에서 ET-1과 TGF-${\beta}_1$의 발현을 살펴보고, 또한 비선택적 ET-1 receptor blocker인 Bosentan이 폐 섬유화의 치료에 효과가 있는지를 보고자 하였다. 방 법 : 웅성 7-8 주령의 백서 120 마리를 세 그룹으로 나누고 제 1그룹은 대조군으로 하여 기관지 내로 생리 식염수를 투여하였고, 제2그룹은 파라콰트를 투여하였으며, 제3그룹은 첫날 파라콰트를 투여한 후 매일 gastric gavage 방법으로 보센탄을 투여하였다. 파라콰트 혹은 생리식염수를 투여한 지 1, 3, 5, 7, 10, 14일째 각각 세 그룹의 일정 수를 희생하여 폐의 병리조직을 보고 면역세포화학염색으로 ET-1과 TGF-${\beta}_1$의 발현 율을 조사하여 분석하였다. 폐 섬유화의 정도는 H&E 염색과 Masson trichrome 염색을 하여 컴퓨터 영상분석을 시행하였고, 면역세포화학염색은 염색정도에 따라 반정량화하여 분석하였다. 결 과 : 파라콰트를 투여한 군이 대조군에 비해 콜라겐의 침착이 실험 3일째부터 현저히 증가하였고, ET-1과 TGF-${\beta}_1$의 발현이 주로 실험 초기에 증가하였다. 그러나 보센탄을 투여한 경우 콜라겐이 침착된 양에는 유의한 변화가 없었고 파라콰트군과 비교해서 ET-1과 TGF-${\beta}_1$의 발현에 뚜렷한 변화는 없었다. 결 론: 파라콰트를 투여한 경우 폐 섬유화가 증가하였다. 그리고 ET-1과 TGF-${\beta}_1$의 발현이 증가하였다. 그러나 ET-1 에 대한 receptor blocker인 보센탄이 폐 섬유화를 막지는 못하였다. 파라콰트에 의한 폐 섬유화에 ET-1이 연관성이 있으나 그 역할에 대해서는 추후 더 연구가 필요할 것으로 사료된다.

• Bulletin of the Korean Chemical Society
• /
• 제33권4호
• /
• pp.1154-1158
• /
• 2012

A room-temperature ionic liquid (RTIL) derived from 1,4-diazabicyclo[2.2.2]octane (DABCO) consisting of tetrafluoroborate anion, 1-butyl-4-(4-sulfonylbutyl)-1,4-diazoniabicyclo[2.2.2]octane hydrogen sulfate tetrafluoroborate ($[C_4DABCOC_4SO_3H][BF_4][HSO_4]$) was synthesized and catalytically evaluated in the synthesis of 14-aryl-14H-dibenzo[a,j]xanthenes by cyclocondensation reaction of ${\beta}$-naphthol and aryl aldehydes. This novel RTIL with an acidic $SO_3H$ group showed high catalytic activity with good to excellent yields of the desired products in short reaction times. Moreover, the catalyst could be recovered and reused at least three times with only slight reduction in its catalytic activity.

• Archives of Pharmacal Research
• /
• 제29권12호
• /
• pp.1074-1079
• /
• 2006

As part of our research on phytochemicals that exert protective effects against diseases related to reactive nitrogen species, we have evaluated the scavenging activity of the yellow leaves of Ginkgo biloba on $ONOO^{-}$. The methanol extract and ethyl acetate fraction obtained from yellow leaves of G. biloba evidenced a marked scavenging activity on authentic $ONOO^{-}$. Repeated column chromatography of the active ethyl acetate soluble fraction on silica gel, Sephadex LH-20, and RP-18, resulted in the purification of 15 known compounds, including sciadopitysin (1), ginkgolide B (2), bilobalide (3), isoginkgetin (4), kaempferol (5), luteolin (6), protocatechuic acid (7), bilobetin (8), amentoflavone (9), ${\beta}-sitosterol$ glucopyranoside (10), kaempferol 3-O-rhamnopyranoside (11), kaempferol 3-O-glucopyranoside (12), kaempferol $3-O-[{6^{'}-O-p-coumaroyl-{\beta}-D-glucopyranosyl(1{\rightarrow}2)-{\alpha}-L-rhamnopyranoside]$ (13), kaempferol 3-O-rutinoside (14), and 6-hydroxykynurenic acid (15). Among the compounds isolated, flavonoids (5, 6 and 11-14), protocatechuic acid (7), and 6-hydroxykynurenic acid (15) all exhibited marked scavenging activities on authentic $ONOO^{-}$. The $IC_{50}$ values of 5-7, 11-14 and 15 were as follows: $2.86{\pm}0.70,\;2.30{\pm}0.04,\;2.85{\pm}0.10,\;5.60{\pm}0.47,\;4.16{\pm}1.65,\;2.47{\pm}0.15,\;3.02{\pm}0.48,\;and\;6.24{\pm}0.27\;{\mu}M$, respectively. DL-Penicillamine ($IC_{50}=4.98{\pm}0.27\;{\mu}M$) was utilized as a positive control. However, the other compounds (1-4, 8-10) exerted no effects against $ONOO^{-}$.

• Molecules and Cells
• /
• 제23권2호
• /
• pp.207-214
• /
• 2007

lant ${\beta}$-1, 3-glucanases are involved in plant defense and in development. Very little data are available on the expression of rice glucanases both in developmental tissues and under various stresses. In this study, we cloned and characterized twenty-seven rice ${\beta}$-1, 3-glucanases (OsGlu) from at total of 71 putative glucanases. The OsGlu genes were obtained by PCR from a cDNA library and were classified into seven groups (Group I to VII) according to their DNA or amino acid sequence homology. Analysis of the expression of the twenty-seven OsGlu genes by Northern blotting revealed that they were differentially expressed in different developmental tissues as well as in response to plant hormones, biotic stress, high salt etc. OsGlu11 and 27 in Group IV were clearly expressed only in stem and leaf and were also induced strongly by SA (5 mM), ABA ($200{\mu}M$), and M. grisea. OsGlu1, 10, 11, and 14 were induced earlier and to higher levels in incompatible M. grisea interaction than in compatible one. Taken together, our findings suggest that the twenty-seven rice OsGlu gene products play diverse roles not only in plant defense but also in hormonal responses and in development.

• 한국안광학회지
• /
• 제14권1호
• /
• pp.47-56
• /
• 2009

목적: 시감도가 최적화된 투과필터의 칼라위치를 추정한다. 방법: 시감도 최적화를 위한 칼라를 Y(Yellow), G(green)과 P(Red)를 이용하여 CR-39에 담그기 법으로 $Y_{1-x}G_{x}$, $Y_{1-x}P_{x}$를 제작하였다. 투과광에서 최종 비시감도의 추정방법 모델은 파장에 따른 눈의 시감도와 렌즈의 투과율 세기의 곱($P_f$ ($P_f({\lambda}=T({\lambda}){\cdot}P({\lambda}).)$.)으로 나타낸다. 400~700 nm 영역에서 투과광의 평가는 비시감도 곡선의 면적, Peak, 폭, 최고높이 값으로 평가한다. 결과: $Y_{1-x}G_{x}$에서 x=0.04, x=0.08, x=0.10, x=0.12, x=0.14, x=0.5에서 ${\beta}=S_1/S_0{\cdot}100$ 값은 각각 76.1, 77.9, 80.7, 81.6, 80.2, 18.6을 갖는다. $Y_{1-x}P_{x}$에서 x=1.00, x=0.2, x=0.6, x=0.8에서 ${\beta}=S_1/S_0{\cdot}100$ 값은 각각 74.3, 74.0, 70.5, 33.0을 갖는다. 투과광의 면적과 최고높이를 평가한 결과는 $Y_{1-x}P_{x}$보다 $Y_{1-x}G_{x}$가 더 큰 값을 갖게 되어 비시감도 최적화에 접근하였다. 결론: 비시감도 최적화는 $Y_{1-x}G_{x}$에서 X=0.12~0.14의 값을 갖는다.