• Archives of Pharmacal Research
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• v.14 no.4
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• pp.290-294
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• 1991

For the determination of peripheral COMT activity, we synthesized $[2-^{14}C]-3',4'-dihydroxyacetophenone([^{14}C]-DHAP)$, a model substrate closely related to catecholamines, which cannot be attacked by monoamine oxidase. After i.v.-injection of $[^{14}C]-DHAP$ in living animals, only 3',4'-dihydroxy-acetophenone (3',4'-DHAP) and 3'-methoxy-4'-hydroxyacetophenone (3'-MHAP) were detected in blood by thin layer radio chromatography. It could be speculated that 3',4'-DHAP was primarily O-methylated by COMT, followed by subsequent conjugations. The concentration of 3',4'-DHAP, a substrate for COMT, in blood at 5 min after injection of $[^{14}C]-DHAP$, were similar in all animals. The rate of 3'-MHAP formation can be therefore used as an indicator for peripheral COMT activity. The velocity of methylation in 15 min after i.v.-administration of $[^{14}C]-DHAP$ was $0.28\;{\mu}g/ml{\cdot}min$. From these results, 3',4'-DHAP was shown to be used as an appropriate substrate to determine the COMT activity in vivo.

• Bulletin of the Korean Chemical Society
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• v.21 no.11
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• pp.1106-1110
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• 2000

New 14-membered tetraaza macrocycles 1,8-diallyl-3,5,7,7,10,12,14,14-octamethyl-1,4,8,11-tetraazacyclotetradecane $(L^2)$ and 1,8-bis(n-propyl)-3,5,7,7,10,12,14,14-octamethyl-1,4,8,11-tetraazacyclotetradecane $(L^3)$ have been prepared by direct react ion of 2,5,5,7,9,12,12,14-octamethyl-1,4,8,11-tetraazacyclotetradecane $(L^1)with$ allyl bromide or n-propyl bromide. The nickel(II) and copper(II) complexes of $L^2andL^3have$ been prepared. The macrocycles show high copper(II) selectivity against nickel(II) ion in methanol solutions containing water. The wavelengths (ca. 505 nm) of the d-d bands for the nickel(II) complexes are extraordinarily longer than those for the complexes of $L^1and$ other related di-N-alkylated 14-membered tetraaza macrocycles. Crystal structure of $[NiL^2](ClO4)_2$ shows that the average Ni-N bond distance $(1.992\AA)$ of the complex is distinctly longer than those of other related nickel(II) complexes. Effects of the N- and C-substituents on the properties of the macrocyclic compounds are discussed.

• Korean Journal of Metals and Materials
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• v.50 no.11
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• pp.855-859
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• 2012

Samples with compositions of 80 wt% Mg-14 wt% Ni-6 wt% $Fe_2O_3$ and 80 wt% Mg-14 wt% Ni-6 wt% Ti were prepared by mechanical grinding under hydrogen (reactive mechanical grinding). Their hydrogen absorptions during reactive mechanical grinding were examined. TGA and BET analysis were employed to investigate the hydrogen storage properties of the prepared alloys. TGA analysis of the $Mg-14Ni-6Fe_2O_3$ showed an absorbed hydrogen quantity of 6.91 wt% while that of Mg-14Ni-6Ti was 2.59 wt%. BET analysis showed that the specific surface areas of $Mg-14Ni-6Fe_2O_3$ and Mg-14Ni-6Ti after reactive mechanical grinding were $264m^2/g$ and $64m^2/g$, respectively. The larger absorbed hydrogen quantity and the larger specific surface area of $Mg-14Ni-6Fe_2O_3$ after RMG than those of Mg-14Ni-6Ti after RMG showed that the effects of $Fe_2O_3$ addition are much stronger than those of Ti addition during reactive mechanical grinding.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.11a
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• pp.317-320
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• 2005

More strict radioactive regulations are applied to Korean nuclear power plants (NPPs) since ICRP-60 recommendation for radiation protection and has been enforced since 2003. In particular. carbon-14 and tritium concentrations are significantly higher at CANDU reactors compared to PWR reactors and this increases the risk of internal radiation exposure to workers at CANDU NPPs. Thus, it is necessary to estimate the exact amount of internal radiation exposure to workers fur radiological protection at CANDU reactors. In this paper, the current dosimetry method for carbon-14 is analyzed for the establishment of internal dosimetry for carbon-14 at domestic NPPs.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.4
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• pp.377-383
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• 2013

We ran a feeding trial to determine optimal dietary protein and lipid levels for growth of juvenile long snout bullhead Leiocassis longirostris Gunther. Eight experimental diets (P20L7, P20L14, P30L7, P30L14, P40L7, P40L14, P50L7 and P50L14) were formulated to contain 20%, 30%, 40% or 50% protein combined with either 7% or 14% lipid. Three replicate groups of fish (mean mass: 3.9 g/fish) were fed one of the experimental diets ad libitum for 8 weeks. Survival of fish fed the P20L14 diet was lower than that of fish fed the P40L14, P50L7 and P50L14 diets. Growth of fish fed diets containing 7% lipid increased with increasing protein level (up to 50% protein); growth of fish fed diets containing 14% lipid increased with increasing protein level (up to 30% protein). The feed efficiency of fish fed a diet with 50% protein and 7% lipid was higher than that of other groups. Whole body moisture and lipid contents were affected by dietary lipid level but not by dietary protein level. The crude lipid contents of fish fed 14% lipid diets were higher than those fed 7% lipid diets across all protein levels (other than the 50% level). Thus, under our experimental conditions, an increase in dietary protein level improved growth and feed efficiency of fish; a diet containing 50% protein with 7% lipid was optimal for growth and effective feed utilization in juvenile long snout bullhead.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.7 no.1
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• pp.53-60
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• 1996

The 8W hybrid MIC SSPA has been developed in the frequency range from 14.0 GHz to 14.5 GHz for uplink of KOREASAT's earth station. The whole system was designed of two parts with driving amplifier and high power amplifier to simplify the fabrication process. we reduced weight and volum of power amplifier through arranging the bias circuits in the same housing. The realized SSPA has a small signal gain of $26\pm1dB$within 500 MHz bandwidith, and the input and output return losses are over 7dB and 12dB respectively. The output power of 39.0 ~ 39.2dBm is achieved at the 1dB gain compression point of 14 GHz, 14.25 GHz, and 14.5 GHz. That reveals higher power than 8W of design target. The proposed SSPA manufacture techni- ques in this paper can be applied to the implementation of power amplifiers for some radars and SCPC.

• Journal of Korean society of Dental Hygiene
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• v.13 no.5
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• pp.759-767
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• 2013

Objectives : The purpose of this study was to investigate the relationship between the oral health status of elderly people and their oral health-related quality of life. Methods : The subjects were the elderly people over 65 years old in Busan. A total of 479 elderly people participated in the study from general hospital, two research institutes, eight dental clinics, six welfare institutions, sixteen senior centers, and three nursing homes. The oral health impact profile-14 (OHIP-14) was used in the routine dental checkups of the elderly people. Results : Those who had no oral symptoms had a better oral health-related quality of life. Among the subfactors of the OHIP-14, significant differences were shown in functional limitation(p=0.001), physical pain(p<0.001), emotional discomfort(p<0.001), physical disability(p=0.001), emotional disability (p=0.001), social disability(p=0.005), physical handicap(p=0.003) and total OHIP-14(p<0.001). Those who had 18 sound teeth(natural teeth) or more had a better oral health-related quality of life. Among the subfactors of the OHIP-14, significant differences were shown in functional limitation(p<0.001), physical pain(p=0.007), emotional discomfort(p=0.019), physical disability(p=0.018), Emotional disability(p=.032) and total OHIP-14 (p=0.006). Conclusions : The results revealed a close relationship between oral health status and oral health-related quality of life. The number of sound teeth(natural teeth) and frequency of toothbrushing had a more positive influence. Therefore oral health programs for the elderly people can preserve remaining teeth. Toothbrushing is the best way to improve the quality of life in the elderly people.

• BMB Reports
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• v.55 no.12
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• pp.633-638
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• 2022

Liver regeneration is a well-known systemic homeostatic phenomenon. The N⁶-methyladenosine (m⁶A) modification pathway has been associated with liver regeneration and hepatocellular carcinoma. m⁶A methyltransferases, such as methyltransferase 3 (METTL3) and methyltransferase 14 (METTL14), are involved in the hepatocyte-specific-regenerative pathway. To illustrate the role of METTL14, secreted from non-parenchymal liver cells, in the initiation phase of liver regeneration, we performed 70% partial hepatectomy (PH) in Mettl14 heterozygous (HET) and wild-type (WT) mice. Next, we analyzed the ratio of liver weight to body weight and the expression of mitogenic stimulators derived from non-parenchymal liver cells. Furthermore, we evaluated the expression of cell cycle-related genes and the hepatocyte proliferation rate via MKI67-immunostaining. During regeneration after PH, the weight ratio was lower in Mettl14 HET mice compared to WT mice. The expressions of hepatocyte growth factor (HGF) and tumor necrosis factor (TNF)-α, mitogens derived from non-parenchymal liver cells that stimulate the cell cycle, as well as the expressions of cyclin B1 and D1, which regulate the cell cycle, and the number of MKI67-positive cells, which indicate proliferative hepatocyte in the late G1-M phase, were significantly reduced in Mettl14 HET mice 72 h after PH. Our findings demonstrate that global Mettl14 mutation may interrupt the homeostasis of liver regeneration after an acute injury like PH by restraining certain mitogens, such as HGF and TNF-α, derived from sinusoidal endothelial cells, stellate cells, and Kupffer cells. These results provide new insights into the role of METTL14 in the clinical treatment strategies of liver disease.

• Journal of Practical Agriculture & Fisheries Research
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• v.14 no.1
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• pp.119-130
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• 2012

To increasing efficiency of the seed production through GA₃ treatment, treatment of GA₃ 20mg/ℓ at its fourteen leaf stage had an effect on 21% increasing seed yield at leaf lettuce (cv.'Ttugseomjeokchugmyeon') in seed production from 50.9ℓ/10a to 61.7ℓ/10a, and 'GA₃ 50mg/ℓ at its eight leaf and fourteen leaf stage had a effect on 14% increasing the seed yield of head lettuce (cv.'Urake') at seed production from 14.2ℓ/10a to 21.1ℓ/10a reducing rate of dead plant in field. Especially, it will be recommended for planting after June for seed production of head lettuce owing to decreasing rate of dead by soft rot and stem rot.

• Development and Reproduction
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• v.27 no.4
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• pp.205-211
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• 2023

INTS14/VWA9, a component of the integrator complex subunits, plays a pivotal role in regulating the fate of numerous nascent RNAs transcribed by RNA polymerase II, particularly in the biogenesis of small nuclear RNAs and enhancer RNAs. Despite its significance, a comprehensive mutation model for developmental research has been lacking. To address this gap, we aimed to investigate the expression patterns of INTS14 during zebrafish embryonic development. We generated ints14 mutant strains using the CRISPR/Cas9 system. We validated the gRNA activity by co-injecting Cas9 protein and a single guide RNA into fertilized zebrafish eggs, subsequently confirming the presence of a 6- or 9-bp deletion in the ints14 gene. In addition, we examined the two mutant alleles through PCR analysis, T7E1 assay, TA-cloning, and sequencing. For the first time, we used the CRISPR/Cas9 system to create a model in which some sequences of the ints14 gene were removed. This breakthrough opens new avenues for in-depth exploration of the role of ints14 in animal diseases. The mutant strains generated in this study can provide a valuable resource for further investigations into the specific consequences of ints14 gene deletion during zebrafish development. This research establishes a foundation for future studies exploring the molecular mechanisms underlying the functions of ints14, its interactions with other genes or proteins, and its broader implications for biological processes.