• Title/Summary/Keyword: 12S

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Biodegradation of Saturated Hydrocarbons by Xanthomonas campestris M12 (Xanthomonas campestris M12에 의한 포화 탄화수소의 생분해)

  • Choi, Soon-Young;Lee, Myung-Hye;Hwang, Moon-Ok;Min, Kyung-Hee
    • Korean Journal of Microbiology
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    • v.32 no.2
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    • pp.172-175
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    • 1994
  • Xanthomonas campestris M12 carrying OCT plasmid which could dissimilate octane was able to utilize n-alkanes of eight to sixteen carbon atoms via the capacity of this plasmid. M12 strain could utilize terminal oxidation products of these primary, alkanes, alcohols, aldehydes and fatty acids but not hexanoic acid, adipic acid, pimelic acid and heptanal. This strain also biodegraded n-alkanes by monoterminal or diterminal oxdation of straight-chain fatty acids, and branched-chain alkane.

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The immunohistochemical studies of Herbal drug-acupuncture of Radix paeoniae lactiflorae on Gastric ulcer induced by HCI-aspirin in Rats (백작약약침(白芍藥藥鍼)이 백서(白鼠)의 위궤양(胃潰瘍)에 미치는 면역조직화학적(免疫組織化學的) 연구(硏究))

  • Park Ki-Hyun;Han Sang-Won;Park Jae-Hyun
    • Journal of Acupuncture Research
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    • v.15 no.2
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    • pp.61-79
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    • 1998
  • In order to study the immunohistochemical effects of herbal drug-acupuncture of Radix paeoniae lactiflorae on gastric ulcer induced by HCl-aspirin in rats. this experiment was done by herbal drug-acupuncture to Wisu($B_{21}$). Chung-Wan($CV_{12}$), Chok-Samni($S_{36}$) loci to measure histological features of ulcer lesion, the change of numbers of parietal cell, chief celI, gastrin and somatostatin-immunoreactive cell. The obtained results were as follows; 1. The ulcerative lesions of gastric mucosa were decreased to WiSU($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 2. In the numbers of parietal cell, the most remarkable decrease was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 3. In the numbers of chief cell, the most remarkable increase was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 4. In the numbers of gastrin-immunoreactive cell, the most remarkable decrease was observed to Wiu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 5. In the numbers of somatostatin-immunoreactive cell, the most remarkable decrease was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups.

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Mitochondrial Genome Sequences of Spirometra erinaceieuropaei and S. decipiens (Cestoidea: Diphyllobothriidae)

  • Eom, Keeseon S.;Park, Hansol;Lee, Dongmin;Choe, Seongjun;Kim, Kyu-Heon;Jeon, Hyeong-Kyu
    • Parasites, Hosts and Diseases
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    • v.53 no.4
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    • pp.455-463
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    • 2015
  • The present study was performed to compare the mitochondrial genomes between 2 Spirometra tapeworms, Spirometra erinaceieuropaei and Spirometra decipiens (Cestoidea: Diphyllobothriidae), which larval stages are important etiological agents of sparganosis in humans. For each species, the full mitochondrial genome was amplified in 8 overlapping fragments using total genomic DNA purified from a single worm as the template. The mitochondrial genomes were 13,643 bp (S. erinaceieuropaei) and 13,641 bp (S. decipiens) in length and contained 36 genes; 12 protein-coding genes, 2 ribosomal RNA (rRNA, small and large subunits), and 22 transfer RNAs (tRNAs). The 12 protein-coding genes constituted 10,083 bp (S. erinaceieuropaei) and 10,086 bp (S. decipiens) of their respective mitochondrial genomes. The tRNA genes, ranging in length from 56 to 70 bp, were identified based on putative secondary structures such as the typical cloverleaf shape. A total of 23 intergenic sequences, varying from 1 to 204 bp in size, were interspersed in S. erinaceieuropaei (total, 504 bp) and S. decipiens (total, 496 bp) mtDNA. The 12 protein-coding genes of S. erinaceieuropaei and S. decipiens differed by 12.4%, whereas the overall difference in mtDNA sequence between S. erinaceieuropaei and S. decipiens was 12.9%. Thus, from the standpoint of the mitochondrial genome, S. decipiens represents a valid species that can be distinguished from S. erinaceieuropaei.

Isolation and characterization of Bacillus subtilis NO12 from button mushroom substrates (양송이 배지로부터 분리된 Bacillus subtilis NO12의 특성)

  • Kim, Hye Soo;Park, Hyun Young;Lee, Chan-Jung;Kong, Won-Sik;Cho, Soo Jeong
    • Journal of Mushroom
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    • v.15 no.4
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    • pp.249-253
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    • 2017
  • Twelve strains of bacteria with cellulase and xylanase activities were isolated from spent mushroom substrates collected from button mushroom cultivation farm, Buye, Chungcheongnam-do in Korea. Among them, one strain, designated NO12, with higher cellulase and xylanase activities was selected by agar diffusion method. The strain NO12 was identified to be a Bacillus sp. by biochemical characteristics using Bacillus ID kit and MicroLog system. Comparative 16S rDNA gene sequence analysis showed that strain NO12 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rDNA gene sequence similarity of 99.2%. Based on its physiological properties, biochemical characteristics, and phylogenetic distinctiveness, strain NO12 was classified within the genus Bacillus, for which the name Bacillus subtilis NO12 was proposed. The cellulase and xylanase activities of B. subtilis NO12 were slightly increased according to bacterial population from exponential phase to stationary phase in the growth curve for B. subtilis NO12. The xylanase activity continuously increased from the beginning of the exponential phase and exhibited maximum activity in the middle of the exponential phase.

The Effects of TSI(Transaction Specific Investment) on Dependence, Trust, Commitment and Re-Contract Intention

  • Yang, Jeong-Seok;Lee, Sang-Youn
    • Journal of Distribution Science
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    • v.12 no.12
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    • pp.19-26
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    • 2014
  • Purpose - This paper aims to test the effects of Korean food service franchisors' and franchisees' TSI (Transaction Specific Investment) on dependence and trust toward the franchisor and re-contract intention. The study examines the effects of both franchisors' and franchisees' TSI on dependence and trust, as compared with Ganesan (1994). Research design, data, and methodology - Data were collected from 495 Korean food service franchisees and analyzed with structural equation modeling using path analysis through SPSS 18.0 and AMOS 18.0. Results - 1) The franchisor's TSI has positive effects on the franchisee's dependence and trust toward the franchisor. 2) The franchisee's TSI has a positive effect on the franchisee's dependence toward the franchisor. 3) The franchisee's dependence and trust have positive effects on commitment. 4) The franchisee's dependence, trust, and commitment have a positive effect on re-contract intention. Conclusions - The franchisor's and franchisee's TSI affect the franchisee's dependence and trust toward the franchisor. The franchisee's dependence and trust influence commitment and re-contract intention. This has managerial implications for franchisors striving to raise franchisees' re-contract intention.

Analysis of trinucleotide repetitive sequences for Korean patients with spinocerebellar ataxia types 8, 12, and 17

  • Kim, Gu-Hwan;Chung, Sun Ju;Ryu, Ho-Sung;Kim, Jaemin;Lee, Jin-Joo;Choi, Seoung Hoon;Lee, Juyeon;Lee, Beom Hee;Choi, Jin-Ho;Yoo, Han-Wook
    • Journal of Genetic Medicine
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    • v.12 no.1
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    • pp.38-43
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    • 2015
  • Purpose: Spinocerebellar ataxias (SCAs) are progressive neurodegenerative disorders with diverse modes of inheritance. There are several subtypes of SCAs. SCA 8, SCA 12, and SCA 17 are the less common forms of SCAs with limited information available on their epidemiological profiles in Korea. The purpose of this study was to investigate the prevalence of SCA8, SCA12, and SCA17 in Korea. Materials and Methods: Ninety-six unrelated Korean patients were enrolled and showed normal trinucleotide repeats through polymerase-chain reaction (PCR) for the genes ATXN1, ATXN2, ATXN3, CACNA1A, and ATXN7, which correspond to SCA1, SCA2, SCA3, SCA6, and SCA7, respectively. PCR products from patients were further analyzed by capillary electrophoresis using fluorescence labeled primers for the genes ATXN8OS, PPP2R2B, and TBP, which correspond to SCA8, SCA12, and SCA17. Results: Three patients had 104, 97, and 75 abnormal expanded repeats in the ATXN8OS gene, the causative gene for SCA8. None of the patients exhibited abnormal repeats in SCA12 and SCA17. Normal trinucleotide repeat ranges of the cohort in this study were estimated to be 17-34 copies (average, $24{\pm}4copies$) for SCA8, 7-18 copies (average, $13{\pm}3copies$) for SCA12, and 26-43 copies (average, $35{\pm}2copies$) for SCA17. Conclusion: This study demonstrated that SCA8, SCA12, and SCA17 are rare in Korean patients with SCA, and further genetic studies are warranted to enhance the mutation detection rate in the Korean SCA population.

Studies on the Dynamic Surface Tension of GL12 and Anionic Mixtures (N-Dodecanoyl, N-Methyl Glucamine(GL 12)과 음이온 계면활성제 혼합물의 Dynamic Surface Tension에 관한 연구)

  • Ahn, Ho-Jeong;Choi, Kyu-Suk
    • Applied Chemistry for Engineering
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    • v.7 no.1
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    • pp.101-108
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    • 1996
  • The dynamic surface tension of GL12 (easily biodegradable nonionic surfactant and mild to skin), LAS and SLES aqueous solutions and that of mixed surfactant systems were measured by the maximum bubble pressure method at different mixing ratios. The effects of various salt such as NaCl, CsCl and urea on the dynamic surface tension of mixed surfactant systems were also studied. The dynamic surface tension of GL12 was not influenced by the presence of salts. On the contrary, the dynamic surface tensions of anionic surfactants (LAS and SLES) were significantly affected by the salts. In the mixed surfactant systems, the effect of salt increased as the composition of anionic LAS or SLES increased in the GL12/LAS and GL12/SLES mixtures.

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The Behavior of Dopants During the Formation of T$TiSi_2$ in the Poly-Si/Single-Si Substrate with Implanted Impurities (불순물이 주입된 Poly-Si/Single-Si 기판에서 $TiSi_2$ 형성시 Dopants의 기동)

  • 최진성;황유상;강성건;김동원;문환구;심태언;이종길;백수현
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.28A no.12
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    • pp.24-30
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    • 1991
  • As a study to use Ti-silicides as interconnection material, the formation of Ti-silicides and the behavior of dopants were investigated for specimens where dopants are introduced on both single-Si substrate and poly-Si that was deposited on the single-Si. Result showed that stable C54 TiSiS12T formed above $700^{\circ}C$ and the formed TiSiS12T had bad surface roughness. And arsenics were chiefly redistributed in TiSiS12T while boron was accumulated near the interface between TiSiS11T and Si during RTA treatment.

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