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Production of Cell Mass and Monacolin K from Monascus sp. on Rice Solid Culture (Monascus 속 균주의 균체 생산 및 고체배양에 의한 Monacolin K 생산)

  • 정혁준;유대식
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.160-166
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    • 2004

  • The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$PO$_4$, 0.05% The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % $(KH_2PO_4$, 0.05% $MgSO_4{\cdot}7H_2O$, 0.2% L-asparagine, pH 4.5, and the optimal inoculum size and shaking speed were $1.5{\times}10^6$ spores/50 m1 medium and 150 rpm, respectively. On optimal conditions, 4.1 g/l of the cell mass was obtained at 28$^{\circ}C$ for 3 days. The mycelium were inoculated on 500 g of steamed rice using vinyl bag ($30.6{\times}44$ cm) and incubated at $30^{\circ}C$, 85% humidity for 21 days. Lactone form monacolin K was rapidly increased for 2 days and reached highest concentration of monacolin K (2,930 mg/kg) for 15 days, and monacolin K was decreased after 15 days.

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A New Korean Search Pattern of the Operator LIKE (연산자 LIKE의 새로운 한글 탐색 패턴)

  • Park, Sung-Chul;Roh, Eun-Hyang;Park, Young-Chul;Park, Jong-Cheol
    • Journal of KIISE:Databases
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    • v.34 no.3
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    • pp.244-260
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    • 2007

  • The operator LIKE of the database language SQL is a string pattern search operator. By providing the string pattern, the operator can identify column values that match with the string pattern. As a phonetic symbol, each Korean syllable is composed either of a leading sound and a medial sound or of a leading sound, a medial sound, and a trailing sound. As a search pattern of Korean syllables of the operator LIKE, in addition to the traditional Korean search pattern, this paper proposes a new search pattern that is based on leading sounds and medial sounds of Korean. With the new Korean search pattern, Korean syllables having specific leading sounds, specific medial sounds, or both specific leading sounds and medial sounds can be found. Formulating predicates equivalent with the new Korean search pattern by way of existing SQL operators is cumbersome and might cause the portability problem of applications depending on the underlying character set of the DBMS. This paper presents algorithms for the execution of the operator LIKE considering the new Korean search pattern based on the characters that are represented in KS X 1001, which is a Korean standard code for information interchange of Korean and Chinese.

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Role of Trehalose Synthesis in Ralstonia syzygii subsp. indonesiensis PW1001 in Inducing Hypersensitive Response on Eggplant (Solanum melongena cv. Senryo-nigou)

  • Laili, Nur;Mukaihara, Takafumi;Matsui, Hidenori;Yamamoto, Mikihiro;Noutoshi, Yoshiteru;Toyoda, Kazuhiro;Ichinose, Yuki
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.566-579
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    • 2021

  • Ralstonia syzygii subsp. indonesiensis (Rsi, former name: Ralstonia solanacearum phylotype IV) PW1001, a causal agent of potato wilt disease, induces hypersensitive response (HR) on its non-host eggplant (Solanum melongena cv. Senryo-nigou). The disaccharide trehalose is involved in abiotic and biotic stress tolerance in many organisms. We found that trehalose is required for eliciting HR on eggplant by plant pathogen Rsi PW1001. In R. solanacearum, it is known that the OtsA/OtsB pathway is the dominant trehalose synthesis pathway, and otsA and otsB encode trehalose-6-phosphate (T6P) synthase and T6P phosphatase, respectively. We generated otsA and otsB mutant strains and found that these mutant strains reduced the bacterial trehalose concentration and HR induction on eggplant leaves compared to wild-type. Trehalose functions intracellularly in Rsi PW1001 because addition of exogenous trehalose did not affect the HR level and ion leakage. Requirement of trehalose in HR induction is not common in R. solanacearum species complex because mutation of otsA in Ralstonia pseudosolanacearum (former name: Ralstonia solanacearum phylotype I) RS1002 did not affect HR on the leaves of its non-host tobacco and wild eggplant Solanum torvum. Further, we also found that each otsA and otsB mutant had reduced ability to grow in a medium containing NaCl and sucrose, indicating that trehalose also has an important role in osmotic stress tolerance.

  • https://doi.org/10.5423/PPJ.OA.06.2021.0087 인용 PDF KSCI