• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.2
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• pp.115-124
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• 1988

This study was carried out to realize the effect of gibberllic acid$(GA_3)$, 1-naphthaleneacetic acid(NAA) and indole-3-acetic acid(IAA) on the biosynthesis of vitamin C. The relation between carbohydrate metabolism and vitamin C production in soybean sprouts was also investigated. Growth, vitamin C content, protein, galactonolactone dehydrogenase(GLD), ribulose diphosphate carboxylase(RuDpCO) and RNA level in the plastid and cytoplasm were determined. The effects of protein and respiratory inhibitors on the growth and vitamin C production were also examined. The most favourable growth of soybean sprouts was observed at the level of NAA $10^{-8}M,\;IAA10^{-6}M\;and\;GA_3\;10^{-5}M$ in the single treatment, respectively, and also favourable at levels of $GA_3\;10^{-5}M+NAA\;10^{-9}M\;and\;GA_3\;10^{-5}M+IAA\;10^{-9}M$ in the case of mixed treatment. The excellent growth was observed at the level IAA $10^{-6}M$ among all the single and mixed treatments. When the soybean sprouts were treated with NAA $10^{-8}M,\;IAA\;10^{-6}M\;GA_3\;10^{-5}M,\;GA_3\;10^{-8}M+IAA\;10^{-6}M,\;and\;GA_3\;10^{-5}M+IAA\;10^{-9}M$, the maximum growth rate was observed at the level of IAA $10^{-6}M$ and the conten of vitamin C was 24.26mg% which was 1.6 times higher than that of the control. RuDpCO was inhibited by the chloramphenicol at the concentration that did not inhibit the growth but the activities of NADP-GDH, GLD and vitamin C content were not affected. These results showed that the biosynthesis of viamin C had nothing to do with the activity of chloroplastic RNA but with cytoplasm. The highest vitamin C content was found at the the level of IAA $10^{-6}M$, where the GLD activity increased up 1.8 times of the control. The concentration of IAA $10^{-6}M$ promoted the biosynthesis of RNa and protein both in chloroplast and cytoplasm, especially in the cytoplasm. Thus it suggeted that IAA affected vitamin C biosynthesis by regulating RNA level in the cytoplasm. 2,4-Dinitrophenol as an uncoupler of oxidative phosphorylation did not inhibit the vitamin C biosynthesis, however, all of the respiratory inhibitors severely inhibited the growth and vitamin C biosynthesis.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.5 no.3
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• pp.208-215
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• 2000

The estimated total material transports through the Cheju Strait using all data which investigated in 1997 and 1999 are as follows; A large amount of suspended sediments and dissovted inorganic nutrients are carried tothe South Sea through the Cheju Strait by a persistent eastward flow (Cheju Current) from the Y311ow Sea andthe East China Sea. The annual material Oanspous by the Cheju Current are as follows; 22.9${\times}$10$^6$ ton yr$^{-1}$(SS), 0.52${\times}$10$^{10}$ mol yr$^{-1}$ (NH$_4\;^+$), 6.05${\times}$10$^{10}$ mol yr$^{-1}$ (NO$_3\;^-$), 0.36${\times}$10$^{10}$ mol yr$^{-1}$ (PO$_4\;^{3-}$), 10.27${\times}$10$^{10}$ mol yr$^{-1}$ (Si(OH)$_4$). The annual suspended sediment flux per water transport in the Cheju Strait (44.48${\times}$10$^6$ ton yr$^{-1}$ Sv$^{-1}$) is about 1.7 larger than that in the Korean Strait (26.08${\times}$10$^6$ ton yr$^{-1}$ Sv$^{-1}$). The annual nitrate flux per water transport (11.60${\times}$10$^{10}$ mol yr$^{-1}$ Sv$^{-1}$) is about 1.2 larger than that in the Korean Strait (9.72${\times}$10$^{10}$ mol yr$^{-1}$ Sv$^{-1}$) and 2/3 of that by Kuroshio in the East China Sea (18.55${\times}$10$^{10}$ ton yr$^{-1}$ Sv$^{-1}$). It suggests that chemical rich Cheju Current will play a significant role in the biogeochemical processes in the South Sea where the huge land-based waste are introduced.

• Journal of Food Hygiene and Safety
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• v.13 no.4
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• pp.441-447
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• 1998

This study was performed to investigate the changes of amount of S. typhimurium during cooking processes using pork and japchae (a Korean food which is made from meat, vegetables and noodles), and to support a practical application to develop a hazard analysis critical control point (HACCP) model. The pork was purchased in a retail shop, cut ($0.5\;cm\;{\times}\;10\;cm\;{\times}\;10\;cm$, 25 g), tested for Salmonella contamination (results: negative), inoculated with S. typhimurium ($10^{7}\;CFU/g$), then treated in various conditions related to cooking. Mter thawing for 24 hours in various conditions, the number of S. typhimurium was increased to $10^{10}\;CFU/g$ at a refrigerated temperature ($4~10^{\circ}C$), and to $10^{21}\;CFU/g$ at room temperature ($22~29^{\circ}C$). Mter thawing in a microwave oven for 40 seconds, the number of S. typhimurium increased to $10^{8}\;CFU/g$. During the thawing period, the number of S. typhimurium increased over time. At the refrigerated temperature, the number of the bacteria was $10^{10}\;CFU/g$ after 24 hours, $10^{13}\;CFU/g$ after 48 hours, and $10^{20}\;CFU/g$ after 72 hours. At room temperature the number of bacteria reached $10^{11}\;CFU/g$ in 2 hours, $10^{15}\;CFU/g$ in 4 hours, $10^{16}\;CFU/g$ in 8 hours, $10^{18}\;CFU/g$ in 12 hours, and $10^{21}\;CFU/g$ in 24 hours. Mter cooking in a frying pan (150{\pm}7^{\circ}C$) for 3 minutes, the bacterial count was $10^{16}\;CFU/g$. After cooking in hot water for 20 minutes, the bacterial count was $10^{7}\;CFU/g\;at\;60^{\circ}C,\;10^{6}\;CFU/g\;at\;63^{\circ}C,\;and\;10^{4}\;CFU/g\;at\;65^{\circ}C$. The fried pork was mixed with cooked vegetables, noodles, sesame oil, sesame seeds, and seasonings to make Korean japchae. This process took $10{\pm}2$ minutes. The bacterial count in the japchae increased to $10^{7}\;CFU/g$ from the count of $10^{6}\;CFU/g$ of the fried pork before it was mixed with the other ingredients. These results indicate that the amount of S. typhimurium is effected by various different cooking processes. This study can suggest that pork should be cooked in water at over $65^{\circ}C$ for 20 minutes in order to prevent food poisoning, if the pork is contaminated with S. typhimurium. The presence of S. typhimurium in the raw pork is identified in an HA for japchae, and the primary CCP for japchae is inadequate cooking (cooking method and time/temperature). We need to standardize time-temperature-size and amount of pork in cooking japchae, because pork is usually cooked in ordinary frying pans when we make this food.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.6
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• pp.407-412
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• 2001

This study was conducted to find out the effect of slow-release nitrogen fertilizer, such as latex coated urea(LCU) and Meister10(MS10) on direct seeded rice in dry soil(DS). Junghwabyeo, and early maturity rice cultivar was grown on the plots which were treated with None-nitrogen. urea. LCU and MS10 plot. Growth characteristic, yield and yield components were investigated. Nitrogen uptake-efficiency and physico-chemical properties of soil before-after experiment were analyzed. Plant height and number of tillers $m^{-2}$ in LCU and MS10 plot at early grow stages were higher than those in urea plot. Plant height and number of tillers $m^{-2}$ grown on the plot of Ms10 plot were higher than those of LCU plot. The number of seedling $m^{-2}$ were no significant differences among None-N, urea, and MS10 plot in DS. Heading date and leaf color were higher with Urea than LCU and MS10 plot. Culm length in LCU and MS10 plot were longer compared with urea plot, but panicle length was similar among with Urea, LCU and MS10 plot. Number of panicles $m^{-2}$ was greater in order of MS10 > LCU > Urea plot. Yield were greater in order of MS10 > LCU > Urea plot. Nitrogen uptake and nitrogen efficiency were greater in order of MS10 > LCU > urea plot. After the experiment, total content of nitrogen in soil was not changed at all treatments, but pH, P and Si of soil were lower than those of before experiment at all treatments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.4
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• pp.470-476
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• 1986

In order to find out the optimum seeding date of Job's tears, this study was investigated ecological characters, yield components and yield of Jang Seong native variety at 6 seeding dates (March 20, March 30, April 10, April 20, April 30 and May 10). Emergence period and heading period were shortened as the seeding date was late, and the number of leaves on the main stem was increased as the seeding date was early. Maturing period was 63-66 days when we seeded in March 20, March 30, April 10 and April 20, and it was delayed 68-77 days when we seeded on April 3$^{\circ}$ and May 10. Culm length was longest in April 10 seeding (it was 186.6 cm), and it grew short in the early and the late seeding, but there was no significant difference among seeding dates. Stem diameter was thickest in April 10, and April 20 seeding, that is, thickened to 10.1 mm, and it was thin in the early and the late seeding. Productive tiller number per hill was increased most in April 10 and April 20 seeding, that is, increased to 6.2, and it was decreased in the early and the late seeding. 100 grain weight was heaviest in April 10 seeding; it was 10.7 g, and it was light in the early and the late seeding. Ripeness rate was highest in April 10 seeding (87.4%), and it was lowest in May 10 seeding (76.3%). Grain yield was highest in April 10 seeding (602.8kg per l0a); therefore this period is regarded as optimum seeding date. C. protein, C. fat, C. fiber and C. starch showed significant difference among seeding dates, and C. protein showed possitive correlation (r =0.9811 **) and C. fiber Negative collelation (r =-0.8937*) with seeding dates.

• Korean Journal of Agricultural Science
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• v.14 no.2
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• pp.295-300
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• 1987

This experiment was carried out to review the chemical composition and microbiological aspects of seasonal raw milk collected from the dairy plants in Kangwon, Kyunggi, Seoul, Chungbuk, Chungnam, Jeorbuk, Jeonnam, Kyungbuk and Kyungnam of Korea. The results were summarized as follows: 1. The average composition of raw milk produced during spring season in each area showed moisture 88.00%, protein 3.49%, fat 3.43%, lactose 4.37%, and ash 0.75% but in summer it showed moisture 88.30%. protein 3.28%, fat 3.32%, lactose 4.26%, and ash 0.70%. 2. The microbiological aspects of raw milk in spring season showed viable bacteria counts $3.7{\times}10^6$, coliforms $2.2{\times}10^5$, thermoduric bacteria $1.4{\times}10^5$, thermophiles $1.4{\times}10^5$, psyclrotrophic bacteria $8.2{\times}10^5$, mould and yeast $1.9{\times}10^4$ per milliliter but in summer it showed viable bactteria counts $3.7{\times}10^6$, coliforms $2.3{\times}10^5$, thermoduric bacteria $2.3{\times}10^5$, thermophiles $7.5{\times}10$, psychrotrophic bacteria $5.0{\times}10^5$, mould and yeast $1.3{\times}10^4$ per milliliter.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.2
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• pp.105-110
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• 1989

The purpose of this study was centered on investigation of the effects N, P and K application rates on root yield and their available content (catalpol, sugar, ash and extracts) of Ji-whang(Rehmannia glutinosa Lib. var. purpurea Makino), and the current application rates(N 20kg/10a, $P_2O_5\;30kg/10a$, $K_2O\;20kg/10a$) was studied for Ji-whang production in Eui-sung Gun southeastern part of Korea Peninsular. The yields of fresh and dried rhizomas were 941-1,494 kg/10a and 251-385 kg/10a, respectively. The rhizoma yield increased highly with increasing the application amount of nitrogen more than 10 kg/10a, but the effects of phosphorus and potassium were observed only at the application level of 40 kg/10a and 20 kg/10a, respectively. Catalpol contents in rhizoma increased at the plot of 20 kg/10a application of nitrogen, but the effects phosphorus and potassium on the catalpol contents were not observed. Catalpol content in dried root increased with increasing N application more than 10 kg/10a. Fructose contents increased at the plot of 20 kg/10a and more N application, and galactose contents decreased with K application. With increasing N application, ash contents decreased, but ethanol extract contents increased.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.1
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• pp.9-16
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• 1996

In order to understand microbial ecosystem in the fast china sea and Yosu coastal sea horizontal distributions of bacterial flora, Vibrio spp., coliform group, temperature, and salinity in this sea area, were studied 42 sampling stations during 6th-l4th August, 1992. From the results, salinity and temperature were $24.64\~33.78\%_{\circ}$ and $22.53\~29.18^{\circ}C$, respectively. As the open sea far from land goes on, salinity became low while temperature became high. Viable cell counts of bacteral flova, Vibrio spp., and roliform group in Yosu coastal sea water were $1.0\times10^2\~3.0\times10^4/ml,\;0.2\times10\~9.0\times10^3/ml,\;and\;0.3\times10\~3.0\times10^3/ml$ while those of these in the open sea water were $0.4\times10\~2.0\times10^3/ml,\;0.8\times10\~3.0\times10/ml,\;and\;0.9\times10\~1.3\times10/ml$, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.9
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• pp.1292-1299
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• 2011

This research aimed to determine the quality characteristics of kochujang made of sword bean chunggukjang. The effects of chunggukjang addition were compared in products fermented for 90 days. During the fermentation period of kochujang, sword bean chunggukjang was added at 0, 2, 5, 8, and 10%. The moisture content ranged from 40.24 to 42.83% (w/w). After 90 days of fermentation, sodium chloride was at around 10.2 to 10.3%, which was not much different from that of control kochujang (SBC 0) before and after fermentation. The color values were not significantly different between SBC kochujang and traditional kochujang. The microbial counts in 0, 2, 5, 8, and 10% SBC kochujang fermented for 90 days were around $5.42{\times}10^7$ to $9.59{\times}10^7$ CFU/g for aerobic viable cells, $1.14{\times}10^2$ to $9.73{\times}10^2$ CFU/g for yeast, and $8.49{\times}10^2$ to $1.25{\times}10^3$ CFU/g for Bacillus cereus. Sensory evaluation of kochujang showed that the comprehensive preference was 5.40, 5.15, 6.30, 6.10, and 6.95, respectively, for SBC 0, 2, 5, 8, and 10%. In conclusion, the quality difference between SBC and traditional kochujang was not significant, and sensory evaluation of kochujang showed that SBC 10% received the highest score.

• Food Science and Preservation
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• v.16 no.4
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• pp.567-572
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• 2009

We evaluated the microbiological quality of a facility in which freshly cut onions were prepared. The total plate counts on walls, equipment, and raw materials were ND (not detected) to $10^1$ CFU/100 $cm^2$, $10^0{\sim}10^3$ CFU/100 $cm^2$, and $10^3{\sim}10^4$ CFU/g, respectively. No coliforms were detected on walls however, coliforms were detected at concentrations of ND to $10^3$ CFU/100 $cm^2$ and $10^3{\sim}10^4$ CFU/g on equipment and raw materials, respectively. The total plate counts for falling and floating bacteria in the processing plant were ND to $10^0$CFU/plate and $10^1{\sim}10^2$ $CFU/m^3$, respectively. Pathogenic microorganisms such as Escherichia coli, Salmonella spp, Staphylococcus aureus, and Listeria monocytogenes were not detected on walls, equipment, or raw materials. Overall, the results of the study indicate that hygiene control at the fresh-cut processing plant should be improved.