• The Plant Pathology Journal
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• v.39 no.1
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• pp.62-74
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• 2023

Plant pathogenic Pectobacterium species cause severe soft rot/blackleg diseases in many economically important crops worldwide. Pectobacterium utilizes plant cell wall degrading enzymes (PCWDEs) as the main virulence determinants for its pathogenicity. In this study, we screened a random mutant, M29 is a transposon insertion mutation in the metC gene encoding cystathionine β-lyase that catalyzes cystathionine to homocysteine at the penultimate step in methionine biosynthesis. M29 became a methionine auxotroph and resulted in growth defects in methionine-limited conditions. Impaired growth was restored with exogenous methionine or homocysteine rather than cystathionine. The mutant exhibited reduced soft rot symptoms in Chinese cabbages and potato tubers, maintaining activities of PCWDEs and swimming motility. The mutant was unable to proliferate in both Chinese cabbages and potato tubers. The reduced virulence was partially restored by a complemented strain or 100 µM of methionine, whereas it was fully restored by the extremely high concentration (1 mM). Our transcriptomic analysis showed that genes involved in methionine biosynthesis or transporter were downregulated in the mutant. Our results demonstrate that MetC is important for methionine biosynthesis and transporter and influences its virulence through Pcc21 multiplication in plant hosts.

• Tribology and Lubricants
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• v.40 no.1
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• pp.24-27
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• 2024

A monotype valve body for a dual clutch transmission has the potential to reduce costs, weight, and manufacturing time by modularizing various parts, including those of existing solenoid packs and valve bodies, into one through the application of super-precision die casting technology. However, this approach may lead to challenges such as reduced rigidity and increased interference due to modularization and compactness, impacting both product performance due to the reduced weight as well as durability and reliability. Unlike existing products, this approach requires a high-precision thin-wall block to avoid more complicated flow line formation, interference between flow lines, and leaks, as well as a strict quality requirement standard and precise inspections including detection of internal defects. To conduct precise inspections, we built an equivalent model corresponding to a driving distance of 300,000 km. Testing involved simulating actual road loads using a real vehicle and a chassis dynamometer in the FTP-75 mode (EPA Federal Test Procedure). The aim of the study was to establish a vehicle load-based part durability model for manufacturing a mono-type valve body and to develop fundamental technology for part weight reduction through preliminary design by introducing analytical weight reduction technology based on the derived results.

• Journal of the korean academy of Pediatric Dentistry
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• v.27 no.4
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• pp.517-523
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• 2000

Nasopharyngeal closure is a sphincter mechanism between the activities of the soft palate, lateral pharyngeal wall and the posterior pharyngeal wall, which divides the oral cavity and the nasal cavity. It participates in physiological activities such as swallowing, breathing and pronunciation. In case of an error in this mechanism, it is called a nasopharyngeal incompetence. The causes of this error are defects in (1) length, function, posture of the soft palate (2) depth and width of the nasopharynx, (3) activity of the posterior and lateral pharyngeal wall. The purpose of this study is to analyze the nasopharynx of cleft palate patients using lateral cephalograms and at the same time, evaluate the degree of hypernasality of each vowels to find its relationship with nasopharyngeal incompetence. The following results were obtained: 1. The length of the soft palate was markedly short than normal. 2. The adequate ratio was smaller than the normal value. 3. As the adequate ratio decreased, when articulating vowels, anatomic mVPI increased. 4. When articulating each vowels, anatomic VPI was in proportion with the degree of hypernasality. 5. The degree of hypernasality was greater in high vowels(/i/, /u/) than low vowel(/a/). From the above results, it can be concluded that in cleft palate patients, lateral cephalograms can be used effectively in diagnosing and evaluating nasopharyngeal incompetence. The anatomic structure of the nasopharynx has close relation to the degree of hypernasality.

• Journal of the Korean Wood Science and Technology
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• v.17 no.1
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• pp.22-54
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• 1989

Seasonal semi-greenhouse type solar-drying of 2.5cm-and 5.0cm-thick lumber of Quercus aliena Blume and Quercus variailis Blume was carried out to investigate the possibility of solar-drying of wood and to decide the active solar-drying period in Korea. In the active solar-drying period obtained solar-dehumidification, semi-greenhouse type solar-, air- and kiln-drying of 2.5cm -thick lumber of oaks were carried out to analyze drying-rates. -defects, and -yield in each drying-method and to calculate daily total absorbed solar-radiation the solar dryers. The energy balance equations were set up, considering all the energy requirements, to analyze the heat efficiencies of semi-greenhouse type solar and solar-dehumidification-dryer. In a seasonal drying the drying rate of semi-greenhouse type solar-dryer was highest in summer, and greater in fall, spring, and winter in order. Solar-drying time was 45% in summer to 50% in winter of the air-drying rime, and more serious drying-defects occurred in air-drying than in solar-drying. In the active solar-drying period. April, May, and June, the average drying rate in solar-dehumidification-drying was 1.0%/day and greater than 0.8%/day in semi-greenhouse type solar-drying. In solar-dehumidification-drying the time required to dry lumber to 10% moisture content was less than 60 days, and solar-dehumidification-drying showed the highest drying-yield, 65.01%, than the other drying methods. The daily total absorbed solar radiations were 8.51MJ on the roof collector and 6.22 MJ on the south wall collector. In the energy blance 69.48% of total energy input was lost by heat conduction through walls, roof. and floor 11.68% by heat leakage, 0.33% by heating the internal structures of the solar-dryer and 5.38% by air-venting. Therefore the heat efficiency of semi-greenhouse type solar-dryer 13.13%, was lower than that of solar-dehumidification-dryer, 14.04%. Solar-drying of lumber in Korea showed the possibility to reduce the air-drying-time in every season and the efficiency of solar-dehumidification drying was higher than that of semi-greenhouse type solar-drying.

• The Korean Journal of Nuclear Medicine
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• v.25 no.1
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• pp.46-52
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• 1991

Radioaerosol inhalation imaging (RII) has been used in radionuclide pulmonary studies for the past 20 years. The method is well accepted for assessing regional ventilation because of its usefulness, easy fabrication and simple application system. To evaluate its clinical utility in the study of impaired regional ventilation in bronchial asthma, we obtained and analysed RIIs in 31 patients (16 women and 15 men; age ranging 21-76 years) with typical bronchial asthma at the Department of Radiology, Kangnam St. Mary's Hospital, Catholic University Medical college, from January, 1988 to August, 1989. Scintiscans were obtained with radioaerosol produced by a BARC(Bhabha Atomic Reserch Center, India) nebulizer with 15 mCi of $^{99m}Tc-phytate$. The scanning was peformed in anterior, posterior and lateral projections following 5-minute inhalation of radioaerosol on sitting position. The scans were analyzed and correlated with the results of pulmonary function study and the findings of chest radiography. Fifteen patients had concomitant lung perfusion image with $^{99m}Tc-MAA$. Follow-up scans were obtained in 5 patients after bronchodilator therapy. The patients were divided into (1) attack type (4 patients), (2) resistant type (5 patients), (3) remittent type (10 patients) and (4) bronchitic type (12 patients). Chest radiography showed hyperinflation, altered pulmonary vascularity, thickening of the bronchial wall and accentuation of basal interstitial markings in 26 of the 31 patients. Chest radiographs were norma! in the remaining 5 patients. Regardless of type, the findings of RII were basically the same, and characterized by the deposition of radioaerosol in the central parts or in the main respiratory air ways along with mottled nonsegmental ventilation defects in the periphery. Peripheral parenchymal defects were more extensive than that of expected findings from clinical symptoms, pulmonary function test and chest radiograph. Broomstick sign was present in 17 patients. The abnormality of RII was poorly correlated with perfusion scans. In all 5 patients treated with bronchodilators, follow-up study demonstrated a decrease in the degree of radioaerosol deposition in the central air way with improved ventilation defects. This study indicates that RII is a useful technique for the evaluation of regional ventilation abnormality and the effect of treatment with bronchodilators in patients with bronchial asthma.

• Korean Journal of Construction Engineering and Management
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• v.14 no.5
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• pp.55-64
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• 2013

The complexity of constructing medical institutions is higher than that of general buildings, and many change orders in the design and defect repairs in the construction phase are required due to strict government regulations. The priority control of constructions and impact factors of medical institutions were analyzed in this study, and difficulties in the control in the design and construction phase were identified. First, the priority management factors that were identified were as follows: architecture, facilities, and electricity. Second, 1) priority management in constructions and factors resulting in change orders and 2) priority management in constructions involving defect repair were analyzed. Third, the importance recognized by the construction managers were analyzed. The priority management in constructions and factors that were recognized by the construction manager were deducted as having low importance, although there were many change orders and defects. The work of finishing, wall building, joining, office automation and communication function, and lighting were analyzed in the design phase, and waste, the office automation and communication function, ceilings, contamination control, and plumbing were analyzed in the construction phase. The results showed that there will be a decrease in change orders and defects if the concentration of the manager was elevated and priorities were managed.

• Journal of the Korean Institute of Gas
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• v.6 no.1 s.17
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• pp.17-23
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• 2002

This paper introduces the spring-mounted odometer system which maintains the correct contact with the pipe wall and measures the distance along the pipe. The odometer wheel is designed to keep contact to the pipelines inner wall and to generate fifty rectangular pulses per one turn(159.5681mm) during pigging. The pipeline has the defects in various types such as buckles, winkles, cracks, dents, welding point and so on. Specially girth welding points which exist each 12m of the pipeline, much affects the operational environment of the odometer. The measurement error of the distance along the pipe is accumulated, for the measurement error of wheel's circumference and the pipeline inner environment. So, this paper proposes the method for the error compensation based on the analysis of the odometer's behavior around the girth welding point of pipe. The experimental results show that developed odometer system can be used for the intelligent pig with good performances.

• Journal of Periodontal and Implant Science
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• v.29 no.3
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• pp.483-509
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• 1999

Biodegradable barrier membrane has been demonstrated to have guided bone regeneration capacity on the animal study. The purpose of this study is to evaluate the effects of cultured calvarial cell inoculated on the biodegradable barrier membrane for the regeneration of the artificial bone defect. In this experiment 35 Sprague-Dawley male rats(mean BW 150gm) were used. 30 rats were divided into 3 groups. In group I, defects were covered periosteum without membrane. In group II, defects were repaired using biodegradable barrier membrane. In group III, the defects were repaired using biodegradable barrier membrane seeded with cultured calvarial cell. Every surgical procedure were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium(30mg/Kg). After anesthesia, 5 rats were sacrificed by decapitation to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. The membrane were inserted on the artificial bone defect after 3 days of culture. A single 3-mm diameter full-thickness artificial calvarial defect was made in each animal by using with bone trephine drill. After the every surgical intervention of animal, all of the animals were sacrificed at 1, 2, 3 weeks after surgery by using of perfusion technique. For obtaining histological section, tissues were fixed in 2.5% Glutaraldehyde (0.1M cacodylate buffer, pH 7.2) and Karnovsky's fixative solution, and decalcified with 0.1M disodium ethylene diaminetetraacetate for 3 weeks. Tissue embeding was performed in paraffin and cut parallel to the surface of calvaria. Section in 7${\mu}m$ thickness of tissue was done and stained with Hematoxylin-Eosin. All the specimens were observed under the light microscopy. The following results were obtained. 1 . During the whole period of experiment, fibrous connective tissue was revealed at 1week after surgery which meant rapid soft tissue recovery. The healing rate of defected area into new bone formation of the test group was observed more rapid tendency than other two groups. 2 . The sequence of healing rate of bone defected area was as follows ； test group, positive control, negative control group. 3 . During the experiment, an osteoclastic cell around preexisted bone was not found. New bone formation was originated from the periphery of the remaing bone wall, and gradually extended into central portion of the bone defect. 4 . The biodegradable barrier membrane was observed favorable biocompatibility during this experimental period without any other noticeable foreign body reaction. And mineralization in the newly formed osteoid tissue revealed relatively more rapid than other group since early stage of the healing process. Conclusively, the cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of regeneration of artificial bone defects of alveolar bone. This study thus demonstrates a tissue-engineering the approach to the repair of bone defects, which may have clinical applications in clinical fields of the dentistry including periodontics.

• Transactions of Materials Processing
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• v.18 no.6
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• pp.488-493
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• 2009

The very big springback of advanced high strength steel(AHSS) sheets invokes undesired shape defects, which can be generally eliminated by die correction or process parameter control. The springback reduction by controlling the forming process parameters is easy for the application, but limited for the bulky achievement. In this study, the effective die correction method, which obtains the modification of tool shape from the relationship between die design variable and springback, is introduced and is applied to the TWB tool of automotive side rail to show the validity and usefulness. Among the die correction trials repeatedly performed, the first trial is carried out by correcting the tool shape to the opposite direction to the springbacks of several tool sections. Next trials are done by extrapolating the springbacks of among the original tool uncorrected and the tools corrected negative amounts of the springback and by finding tool shapes without springbacks. After the angle of side wall and radius of curvature of horizontal bottom floor are chosen as design variables in the tool design of side rail, the tool shape is corrected 3 times. The accuracy of final shape within the assembly limit of 1mm and the springback reduction of 75.8% compared to the uncorrected tool are achieved.

• Journal of Welding and Joining
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• v.30 no.4
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• pp.24-30
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• 2012

Laser welding by fiber laser accompanied by a lot of spatter and humping bead. This is because the deep and narrow keyhole usually form due to high beam quality. So the weld bead is formed defects, because the plasma jet with a high vapor pressure make the molten pool on keyhole wall scattered. For such a reason, unstable behavior of keyhole is difficult to monitor laser welding by using the laser induced plasma. Mostly, fiber laser welding of thick plates most be influenced by this effect. Therefore, fiber laser welding has been difficult to apply the sole. Thus, laser welding monitoring based on plasma measurements have much difficulty in measurements and analysis of signal. In this study, influence of the plasma emission signal according to welding speed and laser power in fiber laser welding analysed by using RMS and FFT analysis. We can verify that RMS value of the plasma emission signal changes with welding parameters in fiber laser welding, and aspect ratio greater than 1, the peak of FFT frequency had been moved in accordance with welding parameter.