• Title/Summary/Keyword: 1-dim.analysis

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Anti-tumorigenic Effect of DIM-pPhBr and DIM-pPhF Originating from Cruciferous Vegetables in KB Human Oral Squamous Cell Carcinoma Through Apoptotic Cell Death (사람구강편명상피암세포주인 KB세포에서 십자화과채소 유래물질인 DIM-pPhBr과 DIM-pPhF의 세포사멸유도를 통한 항종양효능에 관한 연구)

  • Cho, Hyun-Tai;Choi, Eun-Sun;Cho, Nam-Pyo;Cho, Sung-Dae
    • Journal of Food Hygiene and Safety
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    • v.26 no.4
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    • pp.398-402
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    • 2011
  • Cruciferous vegetables including diindolylmethane (DIM) have been shown to have anticancer activity. Especially, DIM-pPhBr and DIM-pPhF used in this study was reported to have more effective and less toxic effects than DIM. However, there is no report presenting their anti-tumorigenic activity in oral cancer. In the present study, we examined the effects of DIM-pPhBr and DIM-pPhF on the cell proliferation and apoptosis in KB human oral cancer cells. DIM-pPhBr and DIM-pPhF decreased cell proliferation and induced apoptosis evidenced by western blot analysis, DAPI staining and sub-$G_1$ population. This provides the first evidence that DIM-pPhBr and DIM-pPhF originating from Cruciferous vegetables induce apoptotic cell death in human oral cancer cells to inhibit cancer cell proliferation.

Prediction of random-regression coefficient for daily milk yield after 305 days in milk by using the regression-coefficient estimates from the first 305 days

  • Yamazaki, Takeshi;Takeda, Hisato;Hagiya, Koichi;Yamaguchi, Satoshi;Sasaki, Osamu
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.10
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    • pp.1542-1549
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    • 2018
  • Objective: Because lactation periods in dairy cows lengthen with increasing total milk production, it is important to predict individual productivities after 305 days in milk (DIM) to determine the optimal lactation period. We therefore examined whether the random regression (RR) coefficient from 306 to 450 DIM (M2) can be predicted from those during the first 305 DIM (M1) by using a RR model. Methods: We analyzed test-day milk records from 85,690 Holstein cows in their first lactations and 131,727 cows in their later (second to fifth) lactations. Data in M1 and M2 were analyzed separately by using different single-trait RR animal models. We then performed a multiple regression analysis of the RR coefficients of M2 on those of M1 during the first and later lactations. Results: The first-order Legendre polynomials were practical covariates of RR for the milk yields of M2. All RR coefficients for the additive genetic (AG) effect and the intercept for the permanent environmental (PE) effect of M2 had moderate to strong correlations with the intercept for the AG effect of M1. The coefficients of determination for multiple regression of the combined intercepts for the AG and PE effects of M2 on the coefficients for the AG effect of M1 were moderate to high. The daily milk yields of M2 predicted by using the RR coefficients for the AG effect of M1 were highly correlated with those obtained by using the coefficients of M2. Conclusion: Milk production after 305 DIM can be predicted by using the RR coefficient estimates of the AG effect during the first 305 DIM.

Photoadaptation of Green Sulfur Photosynthetic Bacteria Chlorobium phaeobacteroides

  • Hirabayashi, Hiroki;Ohmura, Satoshi;Ishii, Takasada;Takaichi, Shinichi;Uehara, Kaku
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.364-366
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    • 2002
  • Photoadaptation of Chlorobium (Cb.) phaeobacteroides was investigated under dim and strong light intensity. Absorption spectra of these whole cellIs were different each other. The Soret band intensity and the Qy bandwidth of BChl e in c디l grown under dim light intensity were smaller and more broadened than those under strong light intensity. From HPLC analysis of the pigments, total carotenoid (Car) / bacterochorolphyll (BChl) e ratio of cell increased wi1h increase of light intensities. But camposition of BChl e hamologs almost unchanged. Cb. phaeobacteroides contains 11 kinds of Car including isorenieratene and beta-isorenieratene as major Car. The campositions of Car were different for cells grown under dim and strong light intensities. In conclusion, Cb. phaeobacteroides changes total amount and canposition of Car to adapt various light intensities, while homolog canposition of BChle unchange.

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The Effects on Somatic Cell Score and Milk Components by Days in Milk in Holstein Dairy Cows (홀스타인 젖소의 비유시기별 체세포 수와 우유 성분에 미치는 제요인)

  • Ahn, B.S.;Kie, K.S.;Suh, K.H.;Hur, T.Y.;Yeo, J.M;Lee, H.J.;Jeon, B.S.;Park, S.B.;Kim, H.S.
    • Journal of Animal Science and Technology
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    • v.46 no.6
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    • pp.925-936
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    • 2004
  • The present study was carried out to investigate effects of various factors such as sire, bovine leukemia virus(BLV) carrier/non-carrier, parity, calving month and lactation periods on somatic cell count(SCC) and milk components in dairy cows. The animals calved from January 2001 to March 2004. Milk samples were collected every 30 $\pm$ 5 days in milk(DlM), and somatic cell count and milk components were analysed by Somascope MK2/Lactoscope FTIR Bovine Leukemia Virus(BLV) was detected by ELISA method. The lactation periods were divided into five periods; (1) 30DIM, (2) 31 to 6ODIM, (3) 61 to 120DIM, (4) 121 to 180DIM, and (5) more than 180DIM. The level of SCC and milk components in all lactation periods were significantly affected by sire, parity, calving month, lactation period and BLV carrier/non-carrier. The results suggest that BLV carrier/non-carrier analysis in a herd may be necessary if milk quality is low owing to a high SCC. BLV carrier/non-carrier did not affect milk protein content for all lactation periods.

3-D Structure of a Coronal Jet Seen in Hinode, SDO, and STEREO

  • Lee, Kyoung-Sun;Innes, Davina;Moon, Yong-Jae;Shibata, Kazunari
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.89.1-89.1
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    • 2011
  • We have investigated a coronal jet near the limb on 2010 June 27 by Hinode/X-Ray Telescope (XRT), EUV Imaging Spectrograph (EIS), Solar Optical Telescope (SOT), SDO/Atmospheric Imaging Assembly (AIA), and STEREO. From EUV (AIA and EIS) and soft X-ray (XRT) images we identify the erupting jet feature in cool and hot temperatures. It is noted that there was a small loop eruption in Ca II images of the SOT before the jet eruption. Using high temporal and multi wavelength AIA images, we found that the hot jet preceded its associated cool jet. The jet also shows helical-like structures during the rising period. According to the spectroscopic analysis, the jet structure changes from blue shift to red one with time, implying the helical structure of the jet. The STEREO observation, which enables us to observe this jet on the disk, shows that there was a dim loop associated with the jet. Comparing the observations from the AIA and STEREO, the dim loop corresponds to the jet structure which implies the heated loop. Considering that the structure of its associated active region seen in STEREO is similar to that in AIA observed 5 days before, we compared the jet morphology on the limb with the magnetic fields extrapolated from a HMI vector magnetogram observed on the disk. Interestingly, the comparison shows that the open field corresponds to the jet which is seen as the dim loop in STEREO. Our observations (XRT, SDO, SOT, and STEREO) are well consistent with the numerical simulation of the emerging flux reconnection model.

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Genetic Persistency of First Lactation Milk Yield Estimated Using Random Regression Model for Indian Murrah Buffaloes

  • Geetha, E.;Chakravarty, A.K.;Vinaya Kumar, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.12
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    • pp.1696-1701
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    • 2006
  • A random regression model was applied for the first time for the analysis of test day records and to study the genetic persistency of first lactation milk yield of Indian Murrah buffaloes. Wilmink's Function was chosen to describe the shape of lactation curves. Heritabilities of test day milk yield varied from 0.33 to 0.58 in different test days. The highest heritability was found in the initial test day ($5^{th}$ day) milk yield. Genetic correlations among test day milk yields were higher in the initial test day milk yield and decreased when the test day interval was increased. The magnitude of genetic correlations between test day and 305 day milk yield varied from 0.25 to 0.99. The genetic persistencies of first lactation milk yield were estimated based on daily breeding values using two methods. $P_1$ is the genetic persistency estimated as a summation of the deviation of estimated daily breeding value on days to attain peak yield from each day after days to attain peak yield to different lactation days. $P_2$ is the genetic persistency estimated as the additional genetic yield (gained or lost) from days to attain peak yield to estimated breeding value on different lactation days relative to an average buffalo having the same yield on days to attain peak yield. The mean genetic persistency on 90, 120, 180, 240, 278 and 305 days in milk was estimated as -4.23, -21.67, -101.67, -229.57, -330.06 and -388.64, respectively by $P_1$, whereas by $P_2$ on same days in milk were estimated as -3.96 (-0.32 kg), -23.94 (-0.87 kg), -112.81 (-1.96 kg), -245.83 (-2.81 kg), -350.04 (-3.28 kg) and -407.58 (-3.40 kg) respectively. Higher magnitude of rank correlations indicated that the ranking of buffaloes based on their genetic persistency in both methods were similar for evaluation of genetic persistency of buffaloes. Based on the estimated range of genetic persistency three types of genetic persistency were identified. Genetic correlations among genetic persistency in different days in milk and between genetic persistencies on the same day in milk were very high. The genetic correlations between genetic persistency for different days in milk and estimated breeding value for 305 DIM was increased from 90 DIM to 180 DIM, and highest around 240 DIM which indicates a minimum of 240 days as an optimum first lactation length might be required for genetic evaluation of Indian Murrah buffaloes.

Study of a coronal jet observed by Hinode, SDO, and STEREO

  • Lee, Gyeong-Seon;Innes, Davina;Mun, Yong-Jae
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.1
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    • pp.35.2-35.2
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    • 2011
  • We have investigated a coronal jet near the limb on 2010 June 27 by Hinode/X-Ray Telescope (XRT), EUV Imaging Spectrograph (EIS), SDO/Atmospheric Imaging Assembly (AIA), and STEREO. From EUV (AIA and EIS) and soft X-ray (XRT) images we identify the erupting jet feature in cool and hot temperatures. Using the high temporal and multi wavelength AIA images, we found that the hot jet preceded its associated cool jet and their structures are well consistent with the numerical simulation of the emerging flux-reconnection model. From the spectroscopic analysis, we found that the jet structure changes from blue shift to red one with time, which may indicate the helical structure of the jet. The STEREO observation, which enables us to observe this jet on the disk, shows that there was a dim loop associated with the jet. On the other hand, we found that the structure of its associated active region seen in STEREO is similar to that in AIA observed 5 days before. Based on this fact, we compared the jet morphology on the limb with the magnectic fields extrapolated from a HMI vector magnetogram of this active region observed on the disk. Interestingly, the comparison shows that the open and closed magnetic field configuration correspond to the jet and the dim loop, respectively, as the Shibata's jet model predicted.

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A Study on the Accuracy of Calculating Slopes for Mountainous Landform in Korea Using GIS Software - Focused on the Contour Interval of Source Data and the Resolution - (GIS Software를 이용한 한국 산악 지형의 경사도 산출 정확도에 관한 연구 -원자료의 등고선 간격과 해상력을 중심으로-)

  • 신진민;이규석
    • Spatial Information Research
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    • v.7 no.1
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    • pp.1-12
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    • 1999
  • The DTM(Digital Terrain Model) in GIS(Geographical Information System) shows the elevation from interpolation using data points surveyed. In panoramic flat landform, pixel size, resolution of source data may not be the problem in using DTM However, in mountainous landform like Korea, appropriate resolution accuracy of source data are important factors to represent the topography concerned. In this study, the difference in contour interval of source data, the resolution after interpolation, and different data structures were compared to figure out the accuracy of slope calculation using DTM from the topographic maps of Togyusan National Park Two types of GIS softwares, Idrisi(grid) ver. 2.0 using the altitude matrices and ArcView(TIN) ver. 3.0a using TIN were used for this purpose. After the analysis the conclusions are as follows: 1) The coarser resolution, the more smoothing effect inrepresenting the topography. 2) The coarser resolution the more difference between the grid-based Idrisi and the TIN-based ArcView. 3) Based on the comparison analysis of error for 30 points from clustering, there is not much difference among 10, 20, 30 m resolution in TIM-based Airview ranging from 4.9 to 6.2n However, the coarser resolution the more error for elevation and slope in the grid-based Idrisi. ranging from 6.3 to 10.9m. 4) Both Idrisi and ArcView could net consider breaklines of lanform like hilltops, valley bottoms.

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Molecular Cloning and Characterization of the Rod Opsin Gene in Olive Flounder Paralichthys olivaceus

  • Kim, Jong-Myoung;Kim, Sung-Wan;Kim, Sung-Koo
    • Fisheries and Aquatic Sciences
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    • v.10 no.1
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    • pp.8-15
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    • 2007
  • Rhodopsin, a dim-light receptor, is a model system for the study of G protein-coupled receptors that transduce extracellular signals into cells. To study the molecular mechanisms of visual systems in fish, the rod opsin gene of olive flounder Paralichthys olivaceus was characterized. The full-length P. olivaceus opsin gene was obtained by PCR amplification of genomic DNA, as well as cDNA synthesis. A comparison of clones obtained from both methods indicated that the olive flounder rod opsin gene lacks introns. Sequence analysis of the opsin gene indicated that it contains a 1,056-bp open reading frame encoding 352 amino acids. The deduced amino acid sequence contains features of typical rod opsins, such as sites for Schiff's base formation (K296) and its counterion (E113), disulfide formation (C110 and C187), and palmitoylation (C322 and C323). An opsin sequence alignment showed the highest similarity between P. olivaceus and Solea solea (95.1%), followed by Hippoglossus hippoglossus (94.5%). An opsin phylogenetic tree revealed a close relationship between olive flounder and teleost rod opsins.

Effect of the Interval from Calving to First Insemination and Days Open on the Subsequent Reproductive Performance in Dairy Cows (젖소에서 분만 후 첫 수정 간격 및 임신 간격이 이후의 번식능력에 미치는 영향)

  • Lee, Soo-Chan;Lee, Tae-Ho;Jeong, Jae-Kwan;Kang, Hyun-Gu;Kim, Ill-Hwa
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.435-441
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    • 2013
  • This retrospective study evaluated the effects of the interval from calving to first insemination (ICFI) and days open (DO) on the reproductive performance in dairy cows. In the first analysis, data from 705 cows were grouped based on the ICFI: short (30-60 days, n=217), medium (61-90 days, n=309), and long (91-150 days, n=179). The occurrence of endometritis was greater in the long group than in short and medium groups (p<0.05). The occurrence of ovarian cysts increased with increasing ICFI (p<0.05), while body condition score (BCS) during the 5 month postpartum period was lower with increasing ICFI (p<0.01). The hazard of pregnancy by 365 days in milk (DIM) was lower (hazard ratio [HR]=0.70, p<0.0001) in the long group, but higher (HR=1.41, p<0.0001) in the short group compared with the medium group. In the second analysis, data from 436 cows were grouped based on the DO: short (30-90 days, n=154), medium (91-180 days, n=183), and long (181-360 days, n=99). The occurrence of a retained placenta was greater in the long group than in the medium group (p < 0.05). Ovarian cysts occurred more frequently in medium and long groups than in the short group (p=0.08). BCS was lower in the short group compared with medium and long groups at month 1 postpartum (p<0.05). Milk yield (kg/day) was greater in the medium group compared with the short group at months 2 to 5 postpartum (p<0.05). The hazard of first insemination by 150 DIM was lower in the long group than in the short group (HR=0.73; p<0.02). The hazard of pregnancy by 365 DIM was lower (HR=0.64, p<0.0001) in the long group than in the medium group, while the hazard did not differ between short and medium groups (p>0.05). Moreover, the culling rate was greater in the long group than in the short group (p<0.05). In conclusion, a longer ICFI and DO resulted in reduced reproductive performance in dairy cows, which was attributable to an increase in postpartum diseases, a lower BCS and a greater milk yield.