• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.117-124
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• 2018

The imports of Centella asiatica L. Urban are increasing year-by-year due to the fact that its extract is a raw material used for skin wounds and in cosmetics. However, studies on the cultivation and identification of native C. asiatica species in Korea have been extremely rare. Therefore, this study was conducted in order to investigate the physiological and functional activity of Korean native C. asiatica plant cultivated in Hapcheon, Gyeongsangnam-do, Korea. As a result, the highest antibacterial and anti-inflammatory activities were examined with methanol extract while skin-whitening and wrinkle improvement were examined with water extract. Seven bacterium and one fungus were treated with 50% methanol extracts of C. asiatica and most of the bacterium showed similar or low levels of antibacterial activity compared to the control group of Omiza (Schisandra chinensis) extract, except for Streptococcus pyogenes, which showed higher antimicrobial activity than that of Omiza extract. However, neither C. asiatica and Omiza extracts showed antimicrobial activity against the fungus, C. albicans. The results of anti-inflammatory effect analyses with Raw 264.7 cells confirmed that the treatment of methanol extract reduced the level of NO by 50% or more compared to the control group. In addition, the water extract showed the highest reduction of melanin content of up to 20% more than the control group when examined with B16F10 cell line, indicating a significant skin-whitening effect. Furthermore, we were able to show the significant skin wrinkle improvement caused by C. asiatica extract with NHDF cell as an indicator, but strong cytotoxicity was also observed, suggesting that further studies are necessary.

• Journal of environmental and Sanitary engineering
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• v.18 no.1
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• pp.51-57
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• 2003

Indoor air quality(IAQ) in workplace and residential environments has been concern of people. Recently, Ministry of Environment in Korea has recognized the potential risk on the healthy effect related to indoor air pollution at home. Therefore, the purpose of this study was performed to measure the indoor air pollutants of IAQ at different homes and investigate to compare the perception of IAQ recognition at home from questionnaire survey in Seoul. We estimated the IAQ of selected 6 homes based on site region and housing type. The indoor air pollutants and parameters such as temperature, relative humidity, respirable suspended particulate matter($PM_{10}$), formaldehyde(HCHO), total bacteria counts, carbon monooxide(CO) and carbon dioxide($CO_2$) were monitored for summer and winter. In monitoring results, the respirable suspended particulate matter(($PM_{10}$) and indoor airborne bacteria level of home 5 and 6 were higher than the standard of the public $150{\;}{\mu}g/m^3$ and $500{\;}{\mu}g/m^3$, the level formaldehyde(HCHO) was exceed 0.1 ppm of the standard of Korea at all monitored homes. In statistics analysis, we could find a correlation between the building age and the concentration of CO, TBC were significant at 0.01 level and Relative Humidity was significant at 0.05 level for summer. Finally, the important air pollutants of IAQ in home were HCHO and total bacteria counts(TBC). And we performed a questionnaire survey of 500 people about their awareness for the importance of IAQ in our home during same period. In results, all most response of occupant has recognized the importance of IAQ at home. Therefore, it can be concluded that the IAQ of selected 6 home studied was perceived as acceptable, it is recommended that the government related IAQ was suggested the guideline and control of IAQ problems, and the occupants need to be effort to reduce the exposure of sources to undesirable pollutants.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.10
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• pp.1312-1317
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• 2008

Physicochemical changes were investigated for the shelf-life extension of cultured wild-ginseng roots during storage with various pre-treatments with blanching, CAMICA-SD and DF-100 and treatments with citric acid and vitamin C. The pH of cultured wild-ginseng roots showed the range of $6.06{\sim}6.42$ at $10^{\circ}C$, but showed higher ranges of $6.08{\sim}6.91$ and $6.08{\sim}8.68$ at 20 and $30^{\circ}C$, respectively. Browning index (a/b) was increased with increasing storage temperature, and the index at 10 and $30^{\circ}C$ were 0.405 and 0.469 after 2 weeks, respectively. Browning index and viable cell number of CAMICA-SD pre-treatment showed little changes compared to pre-teatment with blanching or DF-100. When the cultured wild-ginseng roots were treated with 1.0% citric acid and 0.2% DF-100 after pre-treatments with CAMICA-SD, viable cell number was slightly increased to $4.9{\times}10^2CFU/g$ for 3 weeks storage at $10^{\circ}C$. The mixture of citric acid and DF-100 was also used to prevent the growth of microbiology and to reduce browning reaction, especially enzymatic browning reaction. The mixture might effectively extend shelf life of the cultured wild-ginseng roots.

• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.221-232
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• 1984

A series of experiments were carried out in the laboratory and fields to reevaluate the effects of Padan (cartap) to the brown planthopper (BPH). Nilapanata lugens. The $LD_{50}\;land\;LC_{50}$ values for the female and male BPH were determined by the topical application and seedling- dipping/root -soaking methods. The values were differed with the sex and test methods, and the BPH mortality was greatly increased with a rise in temperatures $(25-35^{\circ}C)$. In a viewpoint of honeydew excretion and offsprings produced, there was no any possibility in BPH resurgence at the sublethal exposures of Padan. The BPH mortality to Padan 4 G was greatly low in the pot tests compared with those to diazinon and carbofuran, but in the paddy fields the efficacy of Padan 4G was nearly reversed. A single application of Padan 4G at the rate of 4kg/10a dramatically suppressed the BPH populations in the paddy fields, and the control effect was much more accelerated in the drained paddy field than in the submerged paddy field.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.4
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• pp.372-381
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• 1983

A laboratory experiment was performed to investigate the effects on redox potential of submerged soil by application of CNP herbicide (2, 4, 6-Trichlorophenyl-4-Nitrophenyl ether) with or without rice straw. Two soils, sandy loam and clay loam textured, were incubated for sixty days at a constant temperature, $25^{\circ}C$. Sampling and analysis of pH, Eh and nitrogen mineralization were carried out during the incubation. The results were summarized as follows. 1. The CNP application decreased redox potential and increased soil pH. The higher the concentration of applicated CNP was, the effects on soil Eh and pH were higher. When rice straw was used with CNP, the Eh of soil remarkably decreased, but seperating the individual effects of rice straw and CNP was impossible in this study. 2. Ammonification increased as the concentration of applied CNP increased regardless of rice straw application or not. It was higher in sandy loam soil than clay loam soil. 3. Nitrification decreased as the concentration of applied CNP increased, especially in the sandy loam soil. Rice straw application reduced nitrification.

• The Korean Journal of Mycology
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• v.13 no.3
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• pp.169-177
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• 1985

The studies were carried out to obtain the basic data for maximizing the protoplast yields from the mycelia of P. ostreatus and P. sajor-caju. Some factors affecting the regeneration of the protoplast of both species and the productivity of their reversion were also examined. The maximum yields of protoplasts were obtained from four days cultured mycelia of both species on cellophan membrane placed on the surface of PSA or MCM media in a petri dish. The optimal concentration of lytic enzyme Novozym 234 for protoplast releasing was 5 mg per ml of 0.5 M phosphate buffer solution with 0.6 M sucrose or 0.6 M $MgSO_4$ at pH 6.0. The greatest number of protoplasts was released 3 hours after incubation of the mycelia of P. ostreatus and after 4 hours for the P. sajor-caju in the lytic enzyme solution. Among the osmotic stabilizer solutions tested 0.6 M sucrose and 0.6 M KCl showed the best regeneration rates of the protoplasts of both species. When 0.75 % agar solution was over-layed on the regeneration media immediately after inoculation of the protoplast the regeneration rates were greatly enhanced. The ampicillin added to the agar solution prevented bacteria from infection. The reverted isolates produced the sporophores and basidial spores just like their parents without any mutations when they were cultivated in a broad mouth bottle with sawdust substrates.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.164-170
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• 2011

Amanita hemibapha is a kind of mycorrhizal mushroom which has a relation with host plants and environmental factors. For the purpose of studying the relationship between environmental factors and mushrooms, in this study we conducted to investigate the soil physicochemical properties, the distribution of plants and the diversity of mushrooms in Gwangyang, Chungdo and Daegu. Soil texture in habitate of Amanita hemibapha was Sandy Loam and Sandy Clay Loam which contains a high rate of sand. Soil pH was approximately 5.0 in all study sites. Total nitrogen contents, C/N ratio and available phosphate($P_2O_5$) were the highest at the Chungdo. However, Gwangyang and Chungdo represent similar Cation Exchange Capacity(C.E.C) and Daegu has much less than other areas. Exchangeable cations, $Ca^{2+}$ content was higher at Gwangyang and Chungdo than Daegu and $Na^+$ content was the highest at Chungdo among three sites. Daegu has much $Mg^{2+}$ contents followed by Chungdo and Gwangyang. Interestingly, $K^+$ content listed in reverse order of $Mg^{2+}$ at Gwangyang, Chungdo and Daegu. The main woody plants in study sites are confirmed as Carpinus laxiflora, Quercus mongolica, Q. serrata and Pinus densiflora. Quercus species are found as a common species in three study sites. Lastly, 8 family and 12 species of mushrooms are emerged in Gwangyang, also 8 family and 12 species and 5 family and 10 species are found in Chungdo and Daegu, respectively. Amanita pantherina, Boletus edulis, Tylopilus felleus and Marasmius maximus, which found in study sites, are also kind of mycorrhizal mushroom same as A. hemibaph. By using correspondence analysis, Q. mongolica, Q. serrata, Q. variabilis and C. laxiflora are expected to as a host plant of A. hemibapha.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1315-1321
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• 2006

This study was conducted to investigate antioxidant activity and functional component analysis in different sections of Korean mountain ginseng (seed, leaf, stem and root). The major free amino acids were arginine, proline, $\gamma-amino-n-butyric$ acid, alanine and aspartic acid, and proline showed the highest content 22.98 mg/g in 80% MeOH extract of seed. Contents of $\gamma-amino-n-butyric$ acid and alanine were the highest in 80% MeOH extract of leaf, with 26.04 mg/g and 13.07 mg/g. Aspartic acid showed the highest content 23.42 mg/g in 80% MeOH extract of leaf. The major fatty acids were identified as palmitic acid, oleic acid and linoleic acid by GC. Content of total phenolic compounds were 737 mg% in 80% EtOH extract of seed, and 560 mg% in 80% MeOH extract of seed. The highest contents of ferulic acid and salicylic acid were $875{\mu}g/g\;and\;78{\mu}g/g$ in 80% MeOH extract of leaf, and $\rho-coumaric$ acid was $181{\mu}g/g$ in 80% MeOH extract of stem, respectively. The highest contents of ginsenoside-Rg1, Re, Rd and Rc were $14.5{\mu}g/g,\;48.78{\mu}g/g,\;27.57{\mu}g/g\;and\;4.87{\mu}g/g$ in 80% MeOH extract of leaf, respectively. Antioxidant activities by DPPH were 83.82% in water extract of leaf, 89.74% in 80% EtOH extract of leaf and 88.37% in 80% MeOH extract of leaf, and 92.81% in BHA (200 ppm). These results suggest that Korean mountain ginseng is very important as functional food material.

• Journal of Soil and Groundwater Environment
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• v.21 no.1
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• pp.49-60
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• 2016

Distribution and behavior of baseline soil CO₂ were investigated in a candidate geologic CO₂ storage site in Pohang, with measuring CO₂ concentrations and carbon isotopes in the vadose zone as well as CO₂ fluxes and concentrations through ground surface. This investigation aimed to assess the baseline CO₂ levels and to build the CO₂ monitoring system before injecting CO₂. The gas in the vadose zone was collected using a peristaltic pump from the depth of 60 cm below ground surface, and stored at gas bags. Then the gas components (CO₂, O₂, N₂, CH₄) and δ¹³C_CO2 were analyzed using GC and CRDS (cavity ringdown spectroscopy) respectively in laboratory. CO₂ fluxes and CO₂ concentrations through ground surface were measured using Li-COR in field. In result, the median of the CO₂ concentrations in the vadose zone was about 3,000 ppm, and the δ¹³C_CO2 were in the wide range between −36.9‰ and −10.6‰. The results imply that the fate of CO₂ in the vadose zone was affected by soil property and vegetations. CO₂ in sandy or loamy soils originated from the respiration of microorganisms and the decomposition of C₃ plants. In gravel areas, the CO₂ concentrations decreased while the δ¹³C_CO2 increased because of the mixing with the atmospheric gas. In addition, the relation between O₂ and CO₂, N₂, and the relation between N₂/O₂ and CO₂ implied that the gases in the vadose zone dissolved in the infiltrating precipitation or the soil moisture. The median CO₂ flux through ground surface was 2.9 g/m²/d which is lower than the reported soil CO₂ fluxes in areas with temperate climates. CO₂ fluxes measured in sandy and loamy soil areas were higher (median 5.2 g/m²/d) than those in gravel areas (2.6 g/m²/d). The relationships between CO₂ fluxes and concentrations suggested that the transport of CO₂ from the vadose zone to ground surface was dominated by diffusion in the study area. In gravel areas, the mixing with atmospheric gases was significant. Based on this study result, a soil monitoring procedure has been established for a candidate geologic CO₂ storage site. Also, this study result provides ideas for innovating soil monitoring technologies.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.4
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• pp.189-194
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• 1975

On a newly reclaimed acidic soil, investigation was made to find out the influence of lime and P application at a largeg uantity on the uptake of Zn by corn and its distribution among the leaves, under afactorial combination with two levels of Zn, lime, and P. The results are summarized as following. 1. The application of lime for neutralization of soil significantly reduced the uptake of Zn by corn. 2. Liberal doses of P (5% of P absorption coefficient of soil; 500ppm) applied before sowing did not influence the uptake of Zn. 3. The concentration of Zn in bottom leaves better reflected the Zn uptake status of corn than the upper leaves. The concentration of Zn in bottom leaves responded clearly to the treatments of lime and Zn, while those of upper leaves tended to be constant unresponding to application of lime and Zn. 4. On the present experimental condition, the yield of corn was increased with the increase of P application. But the concentration of P in plant tissue remained constant under different P levels. 5. Application of lime (calcium hydroxide) on low P plots, depressed the yield of corn significantly. It was speculated that the lime applied at large dose made the applied P less available to corn.