Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Analysis of antibacterial, anti-inflammatory, and skin-whitening effect of Centella asiatica (L.) Urban

병풀의 추출용매에 따른 항균, 항염증 활성 및 피부 미백효능 등의 분석

  • Goo, Young-Min (Gyeongnam Oriental Medicinal Herb Institute) ;
  • Kil, Young Sook (Gyeongnam Oriental Medicinal Herb Institute) ;
  • Sin, Seung Mi (Gyeongnam Oriental Medicinal Herb Institute) ;
  • Lee, Dong Yeol (Gyeongnam Oriental Medicinal Herb Institute) ;
  • Jeong, Won Min (Gyeongnam Oriental Medicinal Herb Institute) ;
  • Ko, Keunhee (Gyeongnam Oriental Medicinal Herb Institute) ;
  • Yang, Ki jeung (Gyeongnam Oriental Medicinal Herb Institute) ;
  • Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University) ;
  • Lee, Shin-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
  • 구영민 (경남한방약초연구소) ;
  • 길영숙 (경남한방약초연구소) ;
  • 신승미 (경남한방약초연구소) ;
  • 이동열 (경남한방약초연구소) ;
  • 정원민 (경남한방약초연구소) ;
  • 고건희 (경남한방약초연구소) ;
  • 양기정 (경남한방약초연구소) ;
  • 김윤희 (국립경상대학교 생물교육과 (농업생명과학연구원)) ;
  • 이신우 (국립경남과학기술대학교 생명과학대학 농학.한약자원학부)
  • Received : 2018.05.21
  • Accepted : 2018.06.22
  • Published : 2018.06.30
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Abstract

The imports of Centella asiatica L. Urban are increasing year-by-year due to the fact that its extract is a raw material used for skin wounds and in cosmetics. However, studies on the cultivation and identification of native C. asiatica species in Korea have been extremely rare. Therefore, this study was conducted in order to investigate the physiological and functional activity of Korean native C. asiatica plant cultivated in Hapcheon, Gyeongsangnam-do, Korea. As a result, the highest antibacterial and anti-inflammatory activities were examined with methanol extract while skin-whitening and wrinkle improvement were examined with water extract. Seven bacterium and one fungus were treated with 50% methanol extracts of C. asiatica and most of the bacterium showed similar or low levels of antibacterial activity compared to the control group of Omiza (Schisandra chinensis) extract, except for Streptococcus pyogenes, which showed higher antimicrobial activity than that of Omiza extract. However, neither C. asiatica and Omiza extracts showed antimicrobial activity against the fungus, C. albicans. The results of anti-inflammatory effect analyses with Raw 264.7 cells confirmed that the treatment of methanol extract reduced the level of NO by 50% or more compared to the control group. In addition, the water extract showed the highest reduction of melanin content of up to 20% more than the control group when examined with B16F10 cell line, indicating a significant skin-whitening effect. Furthermore, we were able to show the significant skin wrinkle improvement caused by C. asiatica extract with NHDF cell as an indicator, but strong cytotoxicity was also observed, suggesting that further studies are necessary.

병풀(Centella asiatica L. Urban)은 피부상처의 치료제 등의 원료로 해마다 해외 수입량은 증가하고 있으나 국내의 자생하는 Centella asiatica종의 조사 및 재배에 관한 연구는 극히 미진하다. 따라서 본 연구는 경상남도 합천의 농가에서 재배중인 국내 자생종인 병풀에 대하여 항균성, 항염증 등을 조사하고, 피부의 상처 및 노화와 관련된 미백효능과 주름개선효과 등에 관한 기초 실험을 수행한 결과 항균 및 항염증 효능은 메탄올 추출물 그리고 미백효능은 물로 추출한 경우에 가장 높은 효능을 확인 할 수 있었으며 보다 상세하게 설명하면 다음과 같다. 7종의 세균과 1종의 곰팡이에 대하여 50% 메탄올로 추출한 병풀 추출물을 처리한 결과 대부분의 세균들은 대조군으로 처리한 오미자 추출물군과 비교하여 유사하거나 낮은 수준의 유의한 항균력을 나타내었으나 S. pyogenes은 유일하게 병풀추출물에서만 항균력을 보였다. 곰팡이인 C. albicans는 병풀과 오미자 모두의 처리에 대하여 항균활성을 확인 할 수가 없었다. Raw 264.7세포를 이용한 항염증효능을 조사한 결과 50%메탄올로 추출한 추출물을 농도별로 처리한 결과 대조구에 비하여 50% 이상의 산화질소 생성량이 감소함을 확인 할 수 있었다. 한편 B16F10세포주를 이용한 미백효능을 조사한 결과에서는 물로 추출한 추출물을 처리한 결과 대조구에 비하여 멜라닌의 농도가 약 20%까지 감소하였음을 확인하였다. NHDF세포를 이용하여 병풀추출물을 처리한 결과 주름개선효능의 지표에 해당하는 MMP-1의 급격한 감소율을 나타내는 처리농도(400 ppm)를 확인하였으나 강한 세포독성도 동시에 확인이 되어 향후 추가연구가 필요한 것으로 조사되었다.

Keywords

References

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