• Journal of Life Science
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• v.8 no.5
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• pp.520-525
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• 1998

Effects of plant growth regulators and anti-oxidants for rapid multiplication of Cymbidium kanran were investigated. The best gelling agent was 2.5 g/1 gelrite which needed less quantity (about 28%) and half price than 9 g/1 chemi-cal agar. Undefined edible agar was only a little bit worse than chemical agar in growth, but the price was half as much as the latter. The higher concentration of BA and NAA, the deeper browning of medium that prevented from performing its functions of plant growth regulators. Polyvinylpyrrolidone (M.W. 40,000) was the most effective anti-oxidant other than ascorbic acid, aspartic acid, and rutin in protecting the browning of medium, enhancing the effect of plant growth regulators, and thus prolonging the subculture cycle. Vigorous seedlings were obtained by 0.1∼1.0 mg/1 BA,0.1 mg/1 NAA and 1 g/1 polyvinylpyrrolidone treatments. Therefore, the best result for growth and econo-mic aspects in rhizome culture of Cymbidium kanran were obtained by using MS basal medium with 2.5 g/1 gelrite, 1 g/1 polyvinylpyrrolidone, 0.1∼1.0 mg/1 BA and 0.1 mg/1 NAA.

• Applied Chemistry for Engineering
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• v.1 no.2
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• pp.197-206
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• 1990

1, 2-Disubstituted imidazolines, such as 1-behenoyl-aminoethyl-2-heneicosylimidazoline(BHI), 1-behenoylbis(aminoethyl)-2-heneicosylimidazoline(BBI), and 1-behenoyltris(aminoethyl)-2-henoicosylimidazoline(BTI) were synthesized by reacting 3 kinds of polyalkylene polyamines with behenic acid to provide the softness to imidazoline ring.1, 2-Disubstituted imidazolinium chlorides, such as 1-behenoyl-aminoethyl-1-glycidyl-2-heneicosylimidazolinium chloride(BHIC), 1-behenoylbis(aminoethyl)-1-glycidyl-2-henoicosylimidazolinium chloride(BBIC), and 1-behenoyltris(aminoethyl)-1-glycidyl-2-henoicosylimidazolinium chloride(BTIC) were prepared by quaternizing 1, 2-disubstituted imidazoline compounds. The optimum condition for the preparation of BHI, BBI, and BTI was 8 hrs at $210^{\circ}C$. In the synthesis of BHIC, the completely quaternized product was obtained by reacting BHI-epichlorohydrin with 1:1.3 mole ratio, and mole ratios higher than 1:1.5 were required in the preparation of BBIC and BTIC, BHICS, BBICS, and BTICS softners form BHIC, BBIC, and BTIC was treated to acrylic fiber. It was found that were good softners as the softening property was measured.

• Applied Biological Chemistry
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• v.40 no.6
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• pp.519-524
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• 1997

As an investigation for utilization of ascidian tunic carotenoids as a food color additives, we attempted to collect the volatile thermal degradation compounds from ascidian tunic carotenoids. Oxygenate volatile compounds were extracted by simultaneous distillation and extraction/concentration apparatus and analyzed by gas chromatography and mass spectrometery. Total 63 compounds were identified and some of them were caused by thermal degradation. They included 1,3,5-trimethylbenzene, 3,5,5-trimethyl-3-cyclohexen-1-ol, 3,5,5-trimethyl-3-cyclohexen-1-one, 1,1,2,3-tetramethyl-2-cyclohexen-5-ol, 1,1,2,3-tetramethyl-2-cyclohexen-5-one, 2,3,4,4-tetramethyl-6-hydroxy-2-cyclohexene-1-one, 1,2,3,8-tetrahydro-3,3,6-trimethyl-1-naphtol, dihydroacetinidolide, ${\beta}-ionone$, 2-(1,1,5-trimethyl-3-hydroxy-5-cyclohexen-6-yl)-1-tolylethene, 2,6-dimethyl-8-(1,1,5-trimethyl-3-hydroxy-5-cyclohexen-6-yl)-1,3,5-octatriene-7-yne. Proposed mechanism of formation of some compounds as thermal degradation products of ascidian tunic carotenoids are provided.

• Journal of the Korean Chemical Society
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• v.40 no.7
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• pp.526-530
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• 1996

3-(N-Alkylamino)-4,5-dihydro-1,2,5-thiadiazole 1,1-dioxides have been prepared by the treatment of 3-imino-1,2,5-thiadiazolidine 1,1-dioxides with alkylamines. The selected spectral properties of these heterocycles are detailed, as well as an X-ray crystallographic structure of a representative member of these compounds. 3-(N-Benzylamino)-4,5-dihydro-4-(4'-methoxyphenyl)-1,2,5-thiadiazole 1,1-dioxide crystallyzes in space group $P2_1a$, monoclinic, with $a=22.512(3)(1)\AA$, $b=9.831(2)\AA$, $c=7.194(4)\AA$, $\beta=96.65(2)$, and Z=4.

• BMB Reports
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• v.52 no.6
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• pp.403-408
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• 2019

Dorsoventral patterning of body axis in vertebrate embryo is tightly controlled by a complex regulatory network of transcription factors. Ventx1.1 is known as a transcriptional repressor to inhibit dorsal mesoderm formation and neural differentiation in Xenopus. In an attempt to identify, using chromatin immunoprecipitation (ChIP)-Seq, genome-wide binding pattern of Ventx1.1 in Xenopus gastrulae, we observed that Ventx1.1 associates with its own 5'-flanking sequence. In this study, we present evidence that Ventx1.1 binds a cis-acting Ventx1.1 response element (VRE) in its own promoter, leading to repression of its own transcription. Site-directed mutagenesis of the VRE in the Ventx1.1 promoter significantly abrogated this inhibitory autoregulation of Ventx1.1 transcription. Notably, Ventx1.1 and Xcad2, an activator of Ventx1.1 transcription, competitively co-occupied the VRE in the Ventx1.1 promoter. In support of this, mutation of the VRE down-regulated basal and Xcad2-induced levels of Ventx1.1 promoter activity. In addition, overexpression of Ventx1.1 prevented Xcad2 from binding to the Ventx1.1 promoter, and vice versa. Taken together, these results suggest that Ventx1.1 negatively regulates its own transcription in competition with Xcad2, thereby fine-tuning its own expression levels during dorsoventral patterning of Xenopus early embryo.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.2
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• pp.78-93
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• 2003

To investigate seasonal variation and structure of the microbial community in Kyeonggi Bay, abundance and carbon biomass of nano-and micrzooplankton were evaluated in relation to size fractionated chlorophyll-a concentration, through the monthly interval sampling from December 1997 to November 1998. Communities of nano-and microzooplankton were classified into 4 groups such as heterotrophic nanoflagellate(HNF), ciliates, heterotrophic dinoflagellates(HDF) and zooplankton nauplii. Abundance and carbon biomass of HNF ranged from 380 to 4,370 cells ml-1(average 1,340$\pm$130 cells ml-1) and from 0.63 to 12.4 $\mu\textrm{g}$C 1-1(average 4.35$\pm$0.58 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of ciliates ranged from 331 to 44,571 cells ml-1(average 3,526$\pm$544 cells ml-1) and from 1.3 to 119.7 $\mu\textrm{g}$C 1-1(average 13.7$\pm$3.0 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of HDF ranged from 88 to 48,461 cells 1-1(average 9,034$\pm$2,347 cells 1-1) and from 0.05 to 54.05 $\mu\textrm{g}$C 1-1(average 6.9$\pm$2.4 $\mu\textrm{g}$C 1-1), respectively. Abundance and carbon biomass of zooplankton nauplii ranged from 5 to 546 indiv. 1-1(average 83$\pm$15 indiv. 1-1) and from 0.17 to 43.2 $\mu\textrm{g}$C 1-1(average 6.3$\pm$1.2 $\mu\textrm{g}$C 1-1), respectively. Eash component of microbial biomass was not different from tidal cycle except tintinnids group. Depth integrated nano-and microzooplankton biomass ranged from 124 to 1,635 mgC m-2(average 585$\pm$110 mgC m-2) and was highest in March and May. The relative contribution of each component to the nano-and microzooplankton showed difference according to seasons. Community structure of nano-and microzooplankton was dominated by planktonic ciliate group. During the study period, carbon biomass of nano-and microzooplankton was strongly positively correlated with size fractionated chlorophylla-a. It implied that prey-predator relationship between microzooplankton and phytoplankton was important in the pelagic ecosystem of Kyeonggi Bay.

• YAKHAK HOEJI
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• v.35 no.4
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• pp.308-313
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• 1991

The synthesis of 1-[(2-phenyl-1, 3-benzodioxol-2-yl)methyl]-1H-imidazole derivatives is described starting with 2-bromoacetophenones and catechols. 1-[(2-Phenyl-1, 3-benzodioxol-2-yl)methyl]-1H-imidazole (9-$_{12}$) showed potent broad-spectrum activity, not only against Candida species but also Cr. neofortnans, S. cerevisiae and T. mentagrophytes.

• Pediatric Infection and Vaccine
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• v.19 no.2
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• pp.61-70
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• 2012

Purpose: This study was performed to compare the clinical characteristics of 2009 pandemic influenza A(H1N1) [A(H1N1) pdm09] and seasonal influenza A infection in the pediatric cancer patients. Methods: A retrospective review was performed in the pediatric cancer patients who had confirmed A(H1N1)pdm09 infection at Samsung Medical Center from August 2009 to February 2010. For the comparison, the medical records of pediatric cancer patients with seasonal influenza A from January 2000 to May 2009 were reviewed retrospectively. Results: Eighty-two A(H1N1)pdm09 infections were confirmed in the pediatric cancer patients. Ten patients (12.2%) developed complicated clinical course by lower respiratory infections or extrapulmonary infections; 4 pneumonia, 1 bronchitis, 1 pericarditis with pneumonia, 1 encephalitis with pneumonia, 2 meningitis and 1 pericarditis. Three patients received mechanical ventilator and ICU care. Three pediatric cancer patients (3.7%) died. The risk factors related to complicated A(H1N1)pdm09 infections were date of infection (44-45th week 2009) and nosocomial infection. When comparing with previous seasonal influenza A infections, more prompt and aggressive antiviral therapy was given in A(H1N1)pdm09 infections. Conclusion: The A(H1N1)pdm09 infections caused a various clinical manifestations including fatal cases in pediatric cancer patient during pandemic season. There was no significant difference in clinical course between influenza A(H1N1)pdm09 and seasonal influenza A infections except the antiviral treatment strategy.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.1
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• pp.73-78
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• 2005

It was aimed to promote Cyrtomium falcatum as a material for interior landscape by validating it indoor adaptability in the indoor environment, especially irrigation times, soil treatment and drainage level. Irrigation times were 2 times per week and 7 times per week. Soil treatment and drainage level were carried out drainage-peatmoss : vermiculite : perlite=1 : 1 : 1(D-PVP), peatmoss : vermiculite : perlite=1 : 1 : 1(PVP), drainage-saprolite : leaf mold=1 : 1(D-SL) and saprolite : leaf mold=1 : 1(SL). 1. Top of growth was better with irrigation 7 times per week than irrigation 2 times per week but indoor adaptability was decreased and shown yellowish green. 2. In case of soil treatment, growth was better with saprolite : leaf mold=1 : 1 but indoor growth adaptability was decreased than peatmoss : vermiculite : perlite=1 : 1 : 1. 3. Plant height and blade length were increased under non-drainage treatment but indoor adaptability, number of new fronds and number of sporophyll were decreased under drainage treatment, regardless of irrigation times and soil treatment. 4. Photosynthetic rate(Pn) was the highest in the drainage-peatmoss : vermiculite : perlite=1 : 1 : 1 treatment of irrigation 2 times per week and was the lowest in the saprolite : leaf mold=1 : 1 treatment of irrigation 7 times per week.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.2
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• pp.95-106
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• 1998

A study was made to estimate the economic level(Necon.) of mineral nitrogen and a cutting frequency for the dry matter production of reed canarygrass(Phalaris arundinacea L.) in uncultivated rice paddy during the harvested years in 1993~1995. Annual mineral nitrogen was applied at the levels of 0, 90, 180, 270 and 360 kg $ha^{-1}$ in 3 cuttings, 0, 120, 240, 360 and 480 kg $ha^{-1}$ in 4 cuttings, and 0, 150, 300, 450 and 600 kg $ha^{-1}$ in 5 cuttings, respectively. The results were summarized as follows; 1. The dry matter yields of all cutting frequencies in 1993 were significantly higher than in the other hay years. Mean dry matter yield were 14.40, 13.88 and 15.98 tons $ha^{-1}$ in 3, 4 and 5 cuttings, respectively. 2. Significantly higher matter yields were obtained as 15.37 and 15.80 tons $ha^{-1}$ at the level of 120 kg $ha^{-1}\;cut^{-1}$ in 3 and 4 cuttings, and 14.02~14.08 tons $ha^{-1}$ levels of 90~120 kg $ha^{-1}$ in 5 cuttings, respectively. 3. Higher efficiencies of dry matter production in response to mineral nitrogen application were recorded as 29.7 kg at level of 90 kg $ha^{-1}\;yr^{-1}$ in 3 cuttings, 19.6 kg at level of 240 kg $ha^{-1}\;yr^{-1}$ in 4 cuttings, and 20.1 kg at level of 150 kg $ha^{-1}\;yr^{-1}$ in 5 cuttings, respectively. 4. Significantly higher matter yields appeared as 5.02 tons $ha^{-1}$ at 2nd cut in 3 cuttings, 3.94~4.37 tons $ha^{-1}$ at 2nd and 3rd cut in 4 cuttings, and 3.81~3.58 tons $ha^{-1}$ at 2nd and 3rd cut in 5 cuttings, respectively. 5. The highest values of relative dry matter yield were 40.4% for 2nd cut in 3 cuttings, 34.9% for 3rd cut in 4 cuttings, and 31.5% for 2nd cut in 5 cuttings, respectively. 6. The estimated marginal dry matter yields(Ymar.) were 13.8~14.7 tons $ha^{-1}$ at ranges of economic N level of 228.5~291.9 kg $ha^{-1}\;yr^{-1}$ in 3 cuttings, 13.8~14.2 tons $ha^{-1}$ at ranges of 293.5~335.7 kg $ha^{-1}\;yr^{-1}$ in 4 cuttings, and 12.2~12.8 tons $ha^{-1}$ at ranges of 237.5~302.5 kg $ha^{-1}\;yr^{-1}$ in 5 cuttings, respectively. 7. Maximun dry matter yields(Ymax.) were 17.0 tons at the level of limiting N(Nmax.) of 558.9 kg $ha^{-1}\;yr^{-1}$ in 3 cuttings, 16.1 ton at level of limiting N of 531.4 kg $ha^{-1}\;yr^{-1}$ in 4 cuttings, and 13.9 ton at level of limiting N of 546.3 kg $ha^{-1}\;yr^{-1}$ in 5 cuttings, respectively. 8. Economic N level in all cuts were in the ranges of 42.6~123.8 kg $ha^{-1}$ in 3 cuttings, 27.3~144.1 kg $ha^{-1}$ in 4 cuttings, and 9.3~159.4 kg $ha^{-1}$ in 5 cuttings, respectively. 9. The proper cutting frequency for matter production of reed canarygrass was 3 cuttings during the h harvested years in 1993~1995, due mainly to the higher efficiency of N for the dry matter production.