• Title/Summary/Keyword: 1,2-unit

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Isolation and physiological characteristics of cellulolytic bacteria (섬유소 분해세균의 분리 및 생리적인 특성)

  • Kwon, Oh-Jin;Chung, Yung-Gun
    • Applied Biological Chemistry
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    • v.37 no.4
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    • pp.226-233
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    • 1994
  • Three hundred and one cellulolytic bacterial were isolated from the 148 screening sources such as decomposed wood, soil, compost and leaf mold. Among them, strain KL-6 was found to have the highest of cellulase activity, and identified as species belonged to the genus Cellulomonas. Strain KL-6 was decompose up to 90% of the filter paper (whatman No. 1) substrate within 50 hours, and showed the colony halo formation (11 cm). The activities of CMCase (67 unit/ml), FPase (70 unit/ml) and ${\beta}-glucosidase$ (0.68 unit/ml) were obtained when this strain was cultured for 50 hrs at $30^{\circ}C$. Glucose was not found in detectable amounts at the FP medium. The optimum composition of nutrient medium for the cell growth by strain KL-6 was sucrose 0.5%, yeast extract 0.1%, $(NH_4)_2HPO_4\;0.1%$, $K_2HPO_4\;0.1%$, $MgSO_4{\cdot}7H_2O\;0.01%$, $CaCl_2\; 0.01%$, NaCl 0.6%, $CaCO_3\;0.1%$ and the optimum pH and temperature were 7.0 and $30^{\circ}C$, respectively.

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The Study of Complex RF Unit in WiBro Base Station for Wave 2 Standard (Wave 2 규격을 위한 와이브로 기지국용 일체형 복합 RF unit 연구)

  • Choi, DooHun;Moon, Yon-Tae;Kim, Do-Gyun;Choi, Young-Wan
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.9
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    • pp.1660-1668
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    • 2008
  • The WiBro was adopted to the 3G international standard. By the change of specification from Wave 1 to Wave 2, MIMO technology is applied in order to increase the speed of downlink. By MIMO the RF part of WiBro base station is increased to 2 Tx paths. Therefore, the size of RF part is bigger and material cost is increased. For reducing these demerits, the RF part which is consisted of PA, LNA, and TDD switch is designed to one complex RF unit. Also, the experimental results of the RF unit have been discussed. Since the complex RF unit is more compact than the RF part of Wave 1 base station, it can be used as the RF part of Wave 2 base station with 2T/2R MIMO.

SECTIONAL CURVATURE OF CONTACT C R-SUBMANIFOLDS OF AN ODD-DIMENSIONAL UNIT SPHERE

  • Kim, Hyang-Sook;Pak, Jin-Suk
    • Bulletin of the Korean Mathematical Society
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    • v.42 no.4
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    • pp.777-787
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    • 2005
  • In this paper we study (n + 1)-dimensional compact contact CR-submanifolds of (n - 1) contact CR-dimension immersed in an odd-dimensional unit sphere $S^{2m+1}$. Especially we provide necessary conditions in order for such a sub manifold to be the generalized Clifford surface $$S^{2n_1+1}(((2n_1+1)/(n+1))^{\frac{1}{2}})\;{\times}\;S^{2n_2+1}(((2n_2+1)/(n+1)^{\frac{1}{2}})$$ for some portion (n1, n2) of (n - 1)/2 in terms with sectional curvature.

Expression of Inulinase Gene by Yeast Constitutive Promoters (효모의 구성적 Promoter들에 의한 Inulinase 유전자의 발현)

  • 김연희;남수완
    • Journal of Life Science
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    • v.9 no.2
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    • pp.153-159
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    • 1999
  • To express constitutively the inulinase gene (INUl) of Kluyveromyces marxianus in Saccharomyces cerevisiae, three yeast promoters such as GAPDH, ADH1 and ENO1 were connected upstream of INUl. The resulting plasmids, pYIGP, pADHl-INU, and pENO-INU were introduced to S. cerevisiae SEY2102 host strain, respectively, and then each transformants were selected by staining of colonies on sucrose-agar plate. When the yeast transformants were cultivated on 2$\%$ dextrose media, the total expression levels of inulinase reached to 1.11 unit/mL, 0.88 unit/mL, and 0.69 unit/mL for respective GAPDH, ADH1, and ENO1 promoter systems. On 4% dextrose media, however, the inulinase activities were observed at 2.00 unit/mL for pYIGP, 0.71 unit/mL for pADH1-INU, and 1.40 unit/mL for pENO-INU. This result indicates that the constitutive expression of INUl was significantly affected by the initial concentration of dextrose and the promoter strength was in the order GAPDH, ENO1, and ADH1 promoter at high dextrose concentration. Taking into account the plasmid stability, however, it is suggested that the ENO1 promoter system is more suitable for the INU1 expression on high dextrose medium or in the fed-batch cultivation accumulating ethanol at high level.

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A study on the marginal fit of CAD/CAM 3-unit bridges (CAD/CAM 3-unit bridges의 변연 적합도에 관한 연구)

  • Lee, Ki-Hong;Yeo, In-Sung;Kim, Sung-Hun;Han, Jung-Suk;Lee, Jai-Bong;Yang, Jae-Ho
    • The Journal of Korean Academy of Prosthodontics
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    • v.49 no.2
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    • pp.101-105
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    • 2011
  • The purpose of this study was to assess the marginal fit of three-unit bridges produced using LAVA CAD/CAM (computer-aided design/computer-aided manufacturing) system and conventional PFG in vitro. Materials and methods: #11, 13 resin teeth were prepared on dentiform, then duplicated. Twenty resin models were fabricated, ten for PFG 3-unit bridges and ten for LAVA 3-unit bridges. Each bridge was cemented on the resin model. Marginal discrepancy was measured with stereoscopic microscope (Nikon DS-Fi 1, Nikon, Japan) at a magnification of ${\times}75$. Independent t-test was done for the statistical analysis. Results: The mean marginal discrepancy values and standard deviations of the PFG bridges was $97.1{\pm}18.7\;{\mu}m$ for incisors, $76.6{\pm}21.8\;{\mu}m$ for canines; that of the LAVA bridges was $90.4{\pm}26.7\;{\mu}m$ for incisor, $110.2{\pm}30.2\;{\mu}m$ for canines. The mean marginal discrepancy between PFG and LAVA for incisor did not show significant difference (P<.05). But for canine, the mean marginal discrepancy of PFG bridges was smaller than that of LAVA bridges (P<.05). Conclusion: The LAVA CAD/CAM 3-unit bridges and the PFG 3-unit bridges showed clinically acceptable marginal discrepancy.

Float Zone Growth of Superconduction $Bi_2Sr_2CaCu_2O_8$ Fiber with Halogen Lamp (할로겐 램프 열원을 이용한 초전도성 $Bi_2Sr_2CaCu_2O_8$ Fiber의 Float Zone 성장)

  • 김철진;정준기
    • Korean Journal of Crystallography
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    • v.7 no.2
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    • pp.147-155
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    • 1996
  • Floating Zone Image furnace with halogen lamps as heat source has been made and applied to the growth of high-Tc superconductor Bi2Sr2CaCu2O8. The manufactured crystal growth equipment is composed of holder unit for setting the halogen lamps at the focal point of the elliptical mirror, image furnace with maximum temperature of 1800℃ with 1kW halogen lamps, cooling unit, feeding unit for supplying source material to the molten zone, pulling unit for crystal growth, and the control unit in the range of 2mm/hr -40 mm/hr vertical movement and 15rpm - 12rpm rotation. Bi2Sr2CaCu2O8 fibers have been grown with 300W halogen lamps and characterized with XRD, SEM and EDS. The growth condition was air atmosphere, growth speed 3∼4mm/hr, rotation speed of upper and lower part 20∼25 rpm. The fiber was composed of 20∼25 rpm matrix phase and secondary phases such as (Sr,Ca)CuO2 and (Sr,Ca)2CuO3.

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Structure-antibiotic activity of cecropin A(1-8)-magainin 2(1-12), cecropin A(1-8)-melittin(1-12) hybrid peptides and their analogues studied by NMR spectroscopy

  • Donghoon Oh;Songyub Shin;Joohyun Kang;Hahm, Kyung-soo;Kim, Killyong;Kim, Yangmee
    • Proceedings of the Korean Biophysical Society Conference
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    • 1999.06a
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    • pp.32-32
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    • 1999
  • Cecropin A(1-8)-magainin 2(1-12) and cecropm A(1-8)-melittin(1-12) hybrid peptides were known to have potent antitumor and antibacterial activity. In particular, cecropm A(l-8)-magainin 2(1-12) has powerful antibacterial and antitumor activity with no hemolytic effect.(omitted)

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Changes of Free Sugars, Lipoxygenase Activity and Effects of Chitosan Treatment during Cultivation of Soybean Sprouts (콩나물 재배중 유리당과 Lipoxygenase Activity의 변화 및 이들에 미치는 키토산 처리의 영향)

  • Lee, You-Seok;Lee, Chong-Ouk
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.115-121
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    • 1999
  • Changes of free sugars, lipoxygenase activity and effects of chitosan treatment were analyzed during cultivation of soybean sprout. Free sugars(sucrose, raffinose and stachyose) were more rapidly decomposed in the cotyledon of soybean sprout. Contents of sucrose and raffinose in control group were decreased more rapidly than those of chitosan treated soybean sprouts (CTSS). But the decreasing rate of stachyose was higher in the CTSS. In the hypocotyl, 82% of L-2/3 activity were decreased, whereas 42% of the activity were decreased in the cotyledon after 132 hours of germination. The effect of chitosan treatment on the lipoxygenase activity was more effective on L-2/3 isozyme than L-1. After the germination period of 132 hours, L-2/3 activity of control group was 82.7unit/mg and that of CTSS was 56unit/mg.

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Physicochemical and Sensory Properties of Jinyang-ju Prepared with Glutinous Rice and Nonglutinous Rice (찹쌀과 멥쌀로 제조한 진양주의 이화학적 및 관능적 특성)

  • Park, Yun-Mi;Kim, Seon-Jae;Hwang, In-Sik;Cho, Kwang-Ho;Jung, Soon-Teck
    • Journal of the Korean Society of Food Culture
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    • v.20 no.3
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    • pp.346-351
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    • 2005
  • The physiochemical characteristics and sensory properties of Jinyang-ju was investigated on pH, total acidity, alcohol content, conductivity, dissolved solid, turbidity, reducing sugar content and sensory evaluation. Glutinous rice starter showed the enzyme activity that the ${\alpha}-amylase$ was 121.0 unit, ${\beta}-amylase$ was 40.0 unit and acidic. neutrality protease were 34.2, 23.7 unit, while the nonglutinous rice starter showed ${\alpha}-amylase$ was 156.0 unit, ${\beta}-amylase$ was 45.2 unit, acidic neutrality protease were 9.06, and 0.1 unit, respectively. The pH of the glutinous rice Jinyang-ju and nonglutinous rice Jinyang-ju showed the value of 5.33 and 4.04, total acidity of 2.30% and 2.05%, alcohol(%) of $2.58{\sim}13.5%\;and\;2.51{\sim}15.5%$, total organic acid content(mg%) of 4.35 and 7.63, free sugar of 4.98 mg% and 2.90 mg%, respectively. The sensory evaluation showed that the glutinous rice Jinyang-ju was more acceptable value than nonglutinous rice Jinyang-ju.

Transglucosylation to Stevioside by Cyclodextrin Glucanotransferase from Bacillus sp. (Bacillus속이 생산하는 Cyclodextrin Glucanotransferase에 의한 Stevioside로의 당전이반응)

  • Chun, Sung-Sook;Cho, Young-Je
    • Applied Biological Chemistry
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    • v.47 no.1
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    • pp.41-48
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    • 2004
  • Cyclodextrin glucanotransferase (CGTase) of Bacillus sp. isolated from soil was purified and its enzymological characteristics were investigated. It was found that the production of CGTase reached to the maximum when the strain was cultured in the broth containing 0.1 % albumin, 2% $NH_4Cl$, 2% soluble starch and 0.2% $NH_2PO_4$ for 72 hrs at $37^{\circ}C$. The purity of CGTase was increased by 9.7 folds through purification procedures by the following column chromatography DEAE-cellulose ion exchange chromatography and Sephadex G-100, G-150 gel filtration and its specific activity was 528.0 unit/mg. The optimum pH and temperature for the CGTase activity were 8.0 and $80^{\circ}C$, respectively. The enzyme was stable in pH $8.0{\sim}11.0$ at $60{\sim}80^{\circ}C$. The activity of purified enzyme was inhibited by $Pb^{2+},\;Hg^{2+}$ and $Zn^{2+}$. When CGTase was treated with each 20.5 unit, 41 unit, 205 unit and 410 unit to investigate the transglucosylation to stevioside by purified cyclodextrin glucanotransferase, transglucosylation rate to stevioside was 74.9%, 75.7%, 68.7% and 57.9%. Brown effect was observed above the concentration amounting to 205 unit of our CGTase.