• Korean Journal of Microbiology
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• v.53 no.4
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• pp.251-256
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• 2017

Some rhizobacteria were isolated, that have copper resistance and can confer copper resistance to plants allowing growth under copper stress. Isolated strains Pseudomonas veronii MS1 and P. migulae MS2 produced 0.13 and 0.26 mmol/ml of siderophore, that is a metal-chelating agent, and also showed 64.6 and 77.9% of biosorption ability for Cu in 20 mg/L Cu solution, respectively. Copper can catalyze a formation of harmful free radicals, which may cause oxidative stress in organisms. Removal activity of 1,1-diphenyl-2-picryl hydrazyl radical and antioxidant capacity of strains MS1 and MS2 increased up to 82.6 and 78.1%, respectively compared to those of control at 24 h of incubation. They exhibited 7.10 and $6.42{\mu}mol$ ${\alpha}$-ketobutyrate mg/h of 1-aminocyclopropane-1-carboxylic acid deaminase activity, respectively, which reduced levels of stress hormone, ethylene in plants, and also produced indole-3-acetic acid and salicyclic acid that can help plant growth under abiotic stress. All these results indicated that these copper-resistant rhizobacteria could confer copper resistance and growth promotion to plants.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.4
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• pp.351-358
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• 2016

The purpose of this research was to examine the effect of the ehanol extracts of Sophora japonica L. (S. japonica). flowers, fruits and branches on skin enhancement with assessing anti-oxidative, whitening, and wrinkle enhancement effects. Results showed that l,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities were $17.68{\pm}1.59{\sim}51.40{\pm}1.04$, $27.48{\pm}0.22{\sim}50.89{\pm}0.13$ and $30.79{\pm}0.55{\sim}45.17{\pm}0.83%$, respectively, in 50~1,000 mg/L of concentrations. The capacities of inhibiting tyrosinase of ethanol extracts from S. japonica. flowers, fruits and branches were $0.27{\pm}0.12{\sim}11.38{\pm}0.57$, $0.27{\pm}0.02{\sim}0.82{\pm}0.27$ and $0.09{\pm}0.16{\sim}0.55{\pm}0.27%$, respectively. The capacities of preventing porcine pancreatic elastase (PPE) were $3.70{\pm}1.23{\sim}7.28{\pm}1.01$, $3.06{\pm}2.13{\sim}13.03{\pm}2.99$ and $6.00{\pm}0.96{\sim}9.71{\pm}0.44%$, respectively, in the case of 50~1,000 mg/L of concentrations. It is concluded that the effects of S. japonica. flowers, fruits and branches on skin improvement are varied significantly.

• The Journal of Korean Medicine
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• v.21 no.1
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• pp.77-83
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• 2000

This study investigated the effects of Siegesbeckia glabrescens, an antihypertensive remedy, on the contraction evoked by phenylephrine and KCl in isolated rat thoracic arata, and also analyzed antioxidative status in vitro. Siegesbeckia glabrescens revealed dose-dependent relaxation on phenylephrine(PE)/KCl-induced arterial contraction and more markedly on PE-induced contraction. Siegesbeckia glabrescens reduced malondialdehyde(MDA)levels, Phosphatidyl choline-liposome(PC-OOH) contents, linoleic acid-induced lipid peroxidation and exerted 1,1-diphenyl-2- picryl-hydrazyl(DPPH) radical scavenging effect, in vitro. These results indicated that Siegesbeckia glabrescens doesn't relaxe artery through a blocking α-adrenergic receptor and calcium channel mediated by voltage-operated calcium channel, and it s antioxidative effects may be involved in endothelium-dependent relaxation of arteries via vascular protective properites. (J Korean Oriental Med 2000;21(1):77-83)

• The Korea Journal of Herbology
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• v.21 no.1
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• pp.17-24
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• 2006

Objectives : This study was conducted to compare antioxidant activity of ratio-related Angelicae Radix and Astragali Radix compositions by DPPH radical scavenging activity. Methods : The species of Angelicae Radix was compared as Angelica gigas, A. sinensis and A. acutiloba. Then various compositions of Angelicae Radix with Astragali Radix were prepared with different ratio. Diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity was measured after 10, 20 and 30 minutes. Each extracts were tested by 1, 5, 10, 50, 100, 500 and 1,000 ${\mu}g/ml$ concentrations. Results : The results showed that Angelica gigas, A. sinensis and A. acutiloba scavenged DPPH radical with 66.8, 61.7 and 56.7% in 1,000 ${\mu}g/ml$ concentration, respectively. According to this result, Angelicae Radix and Astragali Hadix compositions with 100:0, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10 and 0:100 ratio were scavenged DPPH radical with 56.3, 49.9, 61.2, 55.7, 54.7, 55.0, 52.8 ,md 50.2% in 1,000 ${\mu}g/ml$ concentration, respectively. The extract of Angelicae Radix and Astragali Radix compositions with 100:0, 10:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:10 and 0:100 ratio scavenged DPPH radical with the $IC_{50}$ being 85,21, 1016.26, 656.51, 785.67 and 956.74 ${\mu}g/ml$ respectively. Conclusion : In conclusion, the extract of Angelicae Radix and Astragali Radix compositions with 1:1 ratio showed dose-dependent DPPH radical scavenging activity.

• Journal of Life Science
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• v.34 no.5
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• pp.296-303
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• 2024

Vinegar is a fermented food product created by fermenting various sugar- and starch-containing ingredients with microorganisms. It contains a variety of organic acids, sugars, amino acids, esters, and other compounds that contribute to its unique sensory properties. Vinegar is known for its potential benefits, including aiding digestion, lowering blood sugar levels, anti-obesity effects, and antioxidant properties. It is also believed to contribute to improving alkaline body conditions. This study was conducted to develop functional dried vinegar powder from naturally fermented vinegars. Unripe apple, brown rice, and black chokeberry (aronia) were fermented using Gluconacetobacter xylinus for 90-180 days. The filtrate vinegar was spray dried with 37.46% maltodextrin, 5% glucose, 1% citric acid, and 0.04% vitamin C. Analysis of the acidity, color difference, water and soluble solid content, and heat stability of dried vinegar (DV) confirmed that spray drying is a suitable method for powder production. Moreover, the DVs exhibited excellent sensory attributes and solubility. Among the DVs, aronia-DV showed the highest 1,1-diphenyl-2-picryl hydrazyl and 2,2-azobis (3- ethylbenzothiazoline-6-sulfonate) radical scavenging activity (36.7% and 75.3%) and reducing power (0.334) at 0.5 mg/ml concentration, respectively. The nitrite scavenging activity was highest in brown unripe apple-DV, followed by aronia-DV and brown rice-DV. In the anti-thrombosis activity assay, aronia-DV showed the highest prothrombin inhibition. The brown rice-DV exhibited lipid accumulation inhibitory activity in 3T3-L1 adipocytes without cell cytotoxicity. Our results suggest the potential for commercialization of dried vinegar, highlighting its diverse benefits and applications.

• Journal of Applied Biological Chemistry
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• v.58 no.1
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• pp.21-24
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• 2015

Antioxidant activities of ethanol extracts from three types of sweet potatoes with distinctive flesh color (white, orange, and purple) were investigated. Total phenolic content of purple-fleshed sweet potato (PF) was always 2- to 5-fold higher than those of white-fleshed (WF) and orange-fleshed sweet potato (OF). 2,2-diphenyl-1-picryl-hydrazyl free radical scavenging activity of 70% ethanol extracts was highest ($EC_{50}$ = 0.179 mg/mL) for PF and lowest ($EC_{50}$ = 2.127 mg/mL) for OF. And then, reducing power was also highest ($EC_{50}$ = 0.236 mg/mL) for PF and lowest ($EC_{50}$ = 1.587 mg/mL) for WF. Antioxidant activities of 70% ethanol extracts were closely related to the amount of total phenolics. These results imply that antioxidant activity of PF is 6.7 to 11.9 times higher than those of WF and OF.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1485-1495
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• 2019

The purpose of this study was to evaluate the antioxidant and anti-inflammatory effects of extracts of callus cultures, pericarp, flesh, fruit of Trapa Japonica. The toxicity of extracts from Trapa Japonica pericarp investigated using the RAW 264.7 cell showed 45.8 ± 1.5% of cell survival rate. And the results of investigation using HaCaT cells showed a 51.1 ± 1.0% cell viability at 100 ㎍/㎖ in the pericarp extract and lower cell viability at higher concentrations. The total content of polyphenol pericarp extract was 213.20 ± 15.78 mg/g, while the total content of flavonoid was 29.30 ± 3.24mg. And the total content of polyphenol callus cultures extract was 205.20 ± 18.97 mg/g, while the total content of flavonoid was 237.4 ± 7.43 mg. With a concentration level of 0.005 ~ 1000 ㎍/㎖ extract of Trapa Japonica pericarp the range of removal of 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radicals was 67.53 ± 1.5 % ~ 75.75 ± 0.5 % respectively and the range of removal of extract of Trapa Japonica callus cultures extract was also 3.1 ± 0.1 % ~ 77.32 ± 0.5 % respectively. As a result of measuring the Nitric Oxide(NO) generation amount of all Trapa Japonica extract 6.25, 12.5, 25, 50 ㎍/㎖ concentration exhibited significant (p < 0.05, p < 0.01) decreases.

• Journal of Mushroom
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• v.19 no.3
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• pp.140-149
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• 2021

Mushroom-derived polysaccharides, which are the primary bioactive constituents, are beneficial for human health. Polysaccharides have immuno-modulation, antitumor, and antioxidant properties. Additionally, they have antiviral properties and protect against chronic radiation stress. In this study, high yield water-soluble polysaccharides were obtained from Pleurotus djamor var. roseus basidiocarps. The crude polysaccharide (CP) was extracted sequentially by hot water and ethanol precipitation. The yield of the brown CPs was 5.6% dw. Diethylaminoethyl cellulose and Sepharose-6B column chromatography of CPs generated several fractions. Total glucan content was determined in all the fractions. The F1 fraction displayed the highest sugar content and was considered as a purified polysaccharide (PP). The total glucan and β-glucan content in the four fractions ranged between 76.85-2.95% and 75.08-1.46%, respectively. The yield of the PPs was 300 mg, and it was obtained as a white powder. The PPs were characterized by Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography. The FTIR spectral details confirmed the presence of a xylopentose polysaccharide. The antioxidant activity of the PPs was evaluated using in vitro 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and superoxide radical scavenging assay. The PPs showed strong DPPH free radical and superoxide anion radical scavenging activities in a dose-dependent manner. Purified PPs free of phenolics, protein, and carbohydrates were mainly responsible for the radical scavenging activity. The data suggest the potential of PPs as natural antioxidants.

• KSBB Journal
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• v.27 no.4
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• pp.222-226
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• 2012

In this study, the effect of gamma irradiation on the microbial contamination and biological activities of tuna cooking juices was investigated. Tuna cooking juice was by-produced during the canning processing, and had various functional components. But, it was shown that the tuna cooking juice was seriously contaminated. Gamma irradiation effectively reduced the microbial population in tuna cooking juice. Also, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity, tyrosinse inhibitory activity, and ACE inhibitory activity of tuna cooking juices were all increased as a result of gamma irradiation. These results suggest that wasted tuna cooking juices can be used as a functional component in the food and cosmetic industries if the irradiation technology were applied.

• KSBB Journal
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• v.26 no.6
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• pp.565-568
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• 2011

In this study, it was investigated the antioxidant activity of laminarin degraded by gamma irradiation. Because the activities of antioxidants have been attributed to various mechanisms, different assay methods have been conducted and compared. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power, and lipid peroxidation inhibitory activity of degraded laminarin were measured and compared with non-degraded. All of these results showed that the antioxidant activity of laminarin degraded by irradiation was increased depending on the absorbed dose. Therefore, gamma irradiation could be an alternative method for the preparation of degraded laminarin with higher antioxidant activity.