• Title/Summary/Keyword: 1,000V 이상

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Factors associated with Cognitive Decline in the Elderly in Community (일 지역사회 노인의 인지기능저하 요인)

  • Kwon, Young-Sook;Paek, Kyung-Shin
    • Journal of Digital Convergence
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    • v.12 no.2
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    • pp.587-594
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    • 2014
  • This study was carried out to look into the cognitive function of the elderly in community and investigate the factors affecting their cognitive decline provide preliminary data so as to help develop a program to maintain and promote cognitive function. With 481 senior citizens aged over 65 in J. city, a survey was conducted on their demographic characteristics, health-related characteristics and depression using structured questionnaires from September 1 through 7, 2011. The collected data were analyzed by descriptive statistics, Chi-square test and logistic regression analysis using IBM SPSS Statistics V. 20. As a result of this study, 40.1% of the subjects showed cognitive decline and the factors related to their cognitive decline turned out to be the level of education (p<.001), age (p=.000), depression and exercise (p<.05). Therefore, intervention programs on depression or exercise should be implemented intensively and in particular, various programs and educations should be provided considering individual differences according to the level of education and age.

Purification and Some Properties of Chitinase from Serratia marcescens JM (Serratia marcescens JM에 의한 Chitinase의 정제와 특성)

  • Lee, Sang Hwan;Yu, Euy-Kyung
    • Journal of the Korean Chemical Society
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    • v.40 no.1
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    • pp.72-80
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    • 1996
  • A chitinase-producing bacterium, Serratia marcescens JM, was isolated from a seashore muds. A chitinase was purified by ammonium sulfate precipitation, affinity adsorption, hydroxylapatite and sephadex G-200 column chromatography. The chitinase obtained from Serratia marcescens JM was purified 42.2 folds with the overall yield of 7.1%. The purified chitinase showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The molecular weight of the enzyme was 59,000 and the apparent kinetic parameters $K_m\;and\;V_{max}$ for the purified chitinase were 5.17 mg/mL and 39.8 unit/mL, respectively. The optimum pH and temperature of the purified chitinase were 7.0 and 50$^{\circ}C$, respectively and the purified enzyme was stable on pH 7.0 up to 50$^{\circ}C$. The enzyme were activated by $Cu^{2+},\;Ca^{2+}\;and\;Mg^{2+}$ and inhibited by $Hg^{2+}$ respectively. In addition, Cysteine increased the chitinase activity and EDTA, MIA, PCMB and SDS inhibited enzyme activities. Major cations, $MG^{2+},\;Ca^{2+},\;K^+\;and\; Na^+$ present in seawater slightly stimulated the chitinase activity.

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Effect of Sterilization by Intense Pulsed Light on Radiation-resistant Bacterium, Micrococcus roseus (방사선 저항세균 Micrococcus roseus의 광펄스 살균 효과)

  • Kim, Bora;Kim, Ae-Jin;Shin, Jung-Kue
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.248-251
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    • 2013
  • The purpose of this study was to investigate the inactivation effect of intense pulsed light (IPL) on Micrococcus roseus, an irradiation-resistant bacterium isolated from laver, and the commercial feasibility of this sterilization method on dried laver. The inactivation of M. roseus in cultivated plates increased with increasing light intensity and treatment time. Approximately 6.6 log CFU/mL reduction of the cell viability was achieved with IPL treatment for 3 min at 1,000 V of light intensity, tailing was not shown. In addition, the inactivation rate of M. roseus increased with increasing pulse number at same light intensity and treatment time. The killing efficiency for M. roseus increased with by decreasing the distance between the light source and the sample surface.

Vegetation Change and Growing Characteristics of Abies koreana Population by Altitude in Georim Valley of Mt. Jiri (지리산 거림계곡 구상나무 개체군의 고도별 식생변화와 생장특성)

  • Kim, Chang-Hwan;Jo, Min-Gi;Kim, Jong-Kab;Choi, Myung-Suk;Chung, Jae-Min;Kim, Ji-Hong;Moon, Hyun-Shik
    • Journal of agriculture & life science
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    • v.46 no.1
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    • pp.63-70
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    • 2012
  • To provide the basic information for preservation and reasonable management plan of Abies koreana population, vegetation structure and growing condition by altitude were analyzed. Six sites($400m^2$) were set up along the Georim valley in Mt. Jiri from 1,000m to 1,500m. The importance value of A. koreana in tree layer was the highest at 1,400m(site V) and 1,500m(site VI), and that of Quercus mongolica in the other site was high. In subtree layer, the importance value of A. koreana was the highest at site VI which located at 1,500m above the level of the sea. In DBH distribution, A. koreana population distributed evenly at all DBH class(below 10cm, 11-20cm, 21-30cm, above 31cm). Since 2000, the amount of tree-ring growth of A. koreana at site I, II, III, IV, V, and VI were 1.002, 0.996, 1.752, 1.850, 1.198, 0.984 mm/yr, which has decreased slowly in time for 2000, except site III.

Hydrogen Sulfide Removal in Full-scale Landfill Gas Using Leachate and Chelated Iron (침출수 및 철킬레이트를 이용한 실규모 매립가스 내 황화수소 제거)

  • Park, Jong-Hun;Kim, Sang-Hyoun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.2
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    • pp.51-56
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    • 2019
  • $H_2S$ is a detrimental impurity that must be removed for upgrading biogas to biomethane. This study investigates an economic method to mitigate $H_2S$ content, combining scrubbing and aeration. The desulfurization experiments were performed in a laboratory apparatus using EDTA-Fe or landfill leachate as the catalyst and metered mixture of 50-52% (v/v) $CH_4$, 32-33% (v/v) $CO_2$ and 500-1,000 ppmv $H_2S$ balanced by $N_2$ using the C city landfill gas. Dissolved iron concentration in the liquid medium significantly affected the oxidation efficiency of sulfide. Iron components in landfill leachate, which would be available in a biogas/landfill gas utilization facility, was compatible with an external iron chelate. More than 70% of $H_2S$ was removed in a contact time of 9 seconds at iron levels at or over 28 mM. The scrubbing-aeration process would be a feasible and easy-to-operate technology for biogas purification.

Preparation of Yogurt from Milk Added with Purple Sweet Potato (자색고구마 첨가 요구르트의 제조 및 특성)

  • 전승호;이상욱;신용서;이갑성;류일환
    • The Korean Journal of Food And Nutrition
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    • v.13 no.1
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    • pp.71-77
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    • 2000
  • New type yogurt base were prepared from milk added with skim milk powder or purple sweet potato, and fermented by lactic acid bacteria (Streptococcus thermophilus and Bifidobacterium infantis, 1:1, v/v). The yogurt proudcts were evaluated for acid production(pH, titratiable acidity), number of viable cell, viscosity, sensory properties, and color value. The composition of some organic acids was also analyzed by GC. The acid production slightly decrerased by addition with purple sweet potato. There was no significant difference in viable cell counts between control (yogurt added with only skim milk powder) and yogurt added with purple sweet potato, and viable cell counts of all samples were above 9.08 log cfu/ml. Viscosity of yogurt added with purple sweet potato(36,800∼46,000 centipoise) was higher than that of yogurt added with only skim milk powder(32,200 centipoise). The overall sensory score of yogurt added with purple sweet potato(38.6%, dry base) was the best of tested yogurt. The major organic acid of yogurt added with purple sweet potato was lactic acid. its content was 0.997∼1.203%. malic acid, succinic acid, oxalic acid, and fumaric acid were analyzed out a little. Lightness and yellowness decreased by addition with purple sweet potato but redness increased. Total color difference($\Delta$E) with yogurt addition with purple sweet potato and only skim milk powder were very high(above 11.46).

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A Study of Zn-coating Weight in Hotdip Galvanizing Process (응용아연(응용亞鉛) 도금공정(鍍金工程)에서 아연부착량(亞鉛附着量)에 관한 연구)

  • Kim, Wan-Chul;Kang, Sung-Kun;Nam, Seung-Eui
    • Journal of Surface Science and Engineering
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    • v.11 no.1
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    • pp.5-13
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    • 1978
  • This experiment intends to study Zn-coating weight through galvanixing process. A through examination has been carried out on the change of steel-composition, galvanizing-temperature, dipping-rime, Fe-loss when other factors were regular. Activation energy needed for Fe-loss was obtained and within this sample, the best condition which has influence on coating weight was found so as to reduce Fe-loss. As Fe-loss was diminidhed, the amount of product of Dross and Zn-using amount per total products could be reduced. The results of experiments are as follow. 1. Zn-coating thickness was increased by the rising Zn-bathtemperature and was the maximum at 8-minutes dipping time. 2. The coating weight was greatly influency by Si. As in other samples, the proportion of increasing was very rate after 20minutes. If the dipping time prolonged, the increasing amounts of Fe-loss are more greatly. 3. Activation Energy needed for Fe-loss was as follows Such numerical values were most equal to those of the activation energy which had influences on the growth of ${\gamma}$-phase. Sample - I = 18,500 Cal/mole Sample - III = 22,000 Cal/mole Sample - V = 42,300 Cal/mole

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Enzyme treatment for clarification of spoiled oriental melon juice (참외 저급과 주스의 청징화를 위한 효소처리 조건)

  • Jang, Se-Jin;Jo, Yong-Jun;Seo, Ji-Hyung;Kim, Ok-Mi;Jeong, Yong-Jin
    • Food Science and Preservation
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    • v.21 no.4
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    • pp.506-511
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    • 2014
  • In Gyeongsangbuk-do seongju area, hundreds of tons of spoiled oriental melon are harvested annually. Therefore, ways to prevent such spoilage are needed. This study was conducted to investigate the quality characteristics of spoiled oriental melon juice after enzyme treatment for the production of oriental melon concentrate. The treatment of the oriental melon juice with three kinds of enzymes with variable concentrations showed the following results. PECE(1), which was compounded pectinase and cellulase at 0.01% (v/v), gave the melon a 0.16 brown color and 0.01 turbidity, and the highest L value of 97.00. The detected free sugar contents were fructose, glucose and sucrose, with the amount of sucrose the highest at roughly 4,000 mg%. The mixture of different enzyme treatments resulted in a 0.15 brown color and 0.01 turbidity at 60 minutes, and the L value was high at 97.25. The enzyme treatment temperatures of $50^{\circ}C$ and $60^{\circ}C$ yielded a low-level brown color and low turbidity, but the L values were high at $60^{\circ}C$ and $70^{\circ}C$. These results showed that 0.01% (v/v) mixing enzyme, i.e., pectinase and cellulose compounded at $60^{\circ}C$ for 60 min, must be used for the production of oriental melon concentrate.

A Study on the Antioxidant and MMPs Protein Expression Inhibitive Effect of Punica granatum L. Extract and Its Stabilization with Liquid Crystal Emulsion (석류추출물의 항산화와 MMPs 단백질 발현 억제 및 액정 유화물에서의 안정화에 관한 연구)

  • Roh, Jin-Sun;Yeom, Hyeon-Ji;Oh, Min-Jeong;Lee, Jin-Young
    • Journal of Life Science
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    • v.31 no.2
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    • pp.164-174
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    • 2021
  • This study confirmed the potential of Punica granatum L. extract for functional activity verification and cosmetic development. The electron-donating ability of Punica granatum L. extract was shown 60.6% at a 1,000 ㎍/ml concentration. Its ABTS+ radical scavenging ability was shown 93.9% at a 1,000 ㎍/ml concentration. Additionally, the inhibitive effects of elastase and collagenase inhibition effects were measured as 30% and 47.2%, respectively, at a 1,000 ㎍/ml concentration. To determine the effect of Punica granatum L. extract on the proliferation of fibroblasts (CCD-986sk), cell viability was measured using a 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. As a result, survival rates of 130% or higher at a 500 ㎍/ml concentration or less were confirmed. According to the results of Western blot with Punica granatum L. extract, the expression inhibition rates of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-3 (MMP-3) were decreased by 23.2%, 81.9%, and 69.2%, respectively, at a 100 ㎍/ml concentration. Based on the results above, O/W liquid crystal cream with 0.1% Punica granatum L. extract was prepared. The stabilities were tested at 4, 25, 45, and 50℃. By checking the pH, change over time, and stability by temperature, it was confirmed that all were stable for one month. Thus, Punica granatum L. extract shows potential as a natural material for cosmetics.

An Analysis on Characteristics of Turbulence Energy Dissipation Rate from Comparison of Wind Profiler and Rawinsonde (연직바람관측장비와 레윈존데의 비교를 통한 난류 에너지 감소률의 특성 분석)

  • Kang, Woo Kyeong;Moon, Yun Seob;Jung, Ok Jin
    • Journal of the Korean earth science society
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    • v.37 no.7
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    • pp.448-464
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    • 2016
  • The purpose of this study is to optimize the parameters related to consensus coherency within the PCL 1300, the operating program of wind profiler, from a validation of wind data between rawinsonde and wind profiler at Chupungryeong ($36^{\circ}13^{\prime}$, $127^{\circ}59^{\prime}$) site in Korea. It is then to analyze the diurnal and seasonal characteristics of the turbulence energy dissipation rate (${\varepsilon}$) in clear and rainy days from March 2009 to February 2010. In comparison of the wind data between wind profiler and rawinsonde during April 22-23, 2010, it was shown in a big error more than $10ms^{-1}$ over the height of 3,000 meters in the zonal (u) and meridional (v) wind components. When removing more than $10ms^{-1}$ in each wind speed difference of u an v components between the two instruments, the correlation coefficients of these wind components were 0.92 and 0.88, respectively, and the root mean square errors were 3.07 and $1.06ms^{-1}$. Based on these results, when the data processing time and the minimum available data within the PCL 1300 program were adjusted as 30 minutes and 60%, respectively, the bias errors were small. In addition, as a result of an analysis of sensitivity to consensus coherency of u and v components within the PCL1300 program, u components were underestimated in radial coherency, instantaneous and winbarbs coherency, whereas v components were overestimated. Finally by optimizing parameters of the PCL1300 program, the diurnal and seasonal means of ${\varepsilon}$ at each height were higher in rainy days than those in clear days because of increasing in the vertical wind speed due to upward and downward motions. The mean ${\varepsilon}$ for clear and rainy days in winter was lower than those of other seasons, due to stronger horizontal wind speed in winter than those in other seasons. Consequently, when the turbulence energy dissipation rates in the vertical wind speed of more than ${\pm}10cm\;s^{-1}$ were excluded for clear and rainy days, the mean ${\varepsilon}$ in rainy days was 6-7 times higher than that in clear days, but when considering them, it was 4-5 times higher.