• 한국축산식품학회지
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• 제31권1호
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• pp.54-60
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• 2011

This study was performed to examine the possibility of water extracts of several digestive medicinal plants (DMPEs), such as Amomum tasoko, Alpinia oxyphylla, Citrus unshiu, and Myristica fragrans, as a natural antioxidant. Total phenol contents of each extract were expressed as gallic acid equibalents (GAE) and those were significantly different among A. tasoko ($39.87{\pm}5.77$ mg GAE/g), A. oxyphylla ($30.28{\pm}3.36$ mg GAE/g), C. unshiu ($28.13{\pm}5.01$ mg GAE/g) and M. fragrans ($6.36{\pm}0.30$ mg GAE/g) (p<0.05), and extract of A. tasoko showed significantly higher antioxidative effect than butylated hydroxyanisole (BHA) on linoleic acid peroxidation at 72 h after incubation (p<0.05). Addition of extracts in pork patties did not affect the pH value and total microbes during cold storage. However, thiobarbituric acid reative substances (TBARS) of treated patties were lower in dose dependant manner than that of control as storage period increased (except patties treated with C. unshiu extract), and patties treated with 0.5% A. tasoko extract showed no significant difference with patties treated with 0.5% BHA at day 7.

• Journal of Ginseng Research
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• 제16권2호
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• pp.111-123
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• 1992

Drying characteristic data for peeled ginseng were obtained to determine dominant drying factors and fitted with five selected drying models and an empirical model. Among air temperature, relative humidity and diameter of ginseng root, drying air temperature was found to be the most influencing factor on drying rate. Drying velocity appeared faster as the drying temperature increased but its effect was less at high temperature than at low temperature. Quality change during the drying process did not occur except when relative humidity was 75fb. At high relative humidity, skin color of ginseng was turned to light brown. Approximate-Diffusion and the Empirical model for drying were in a good agreement with experimental data. The models are as follows; $.$ Approximate-Diffusion model MR = A$.$exe(-k$.$1) A = 1.72 + 0.407 In(D) - 0.0000963T3 - 0.358 In(RH) + 0.0000945 RH2 B= 1.01 + 0.0195RH - 0.O0518D2 + 0.0708 In(T) - 0.492 In(RHI-D.0000933RH2$.$Empirical model MR= Cl + Cs$.$In(t) Cl= 1.14+0.382 In(D)-0.00008477a-0.139 In(RH)+0.0000664RH2 Cs=0.440-0.0224 In(D)-0.193 In(T)+0.0000464T2-0.00000771RH2

• The Plant Pathology Journal
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• 제32권5호
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• pp.431-440
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• 2016

In 2004, bacterial spot-causing xanthomonads (BSX) were reclassified into 4 species-Xanthomonas euvesicatoria, X. vesicatoria, X. perforans, and X. gardneri. Bacterial spot disease on pepper plant in Korea is known to be caused by both X. axonopodis pv. vesicatoria and X. vesicatoria. Here, we reidentified the pathogen causing bacterial spots on pepper plant based on the new classification. Accordingly, 72 pathogenic isolates were obtained from the lesions on pepper plants at 42 different locations. All isolates were negative for pectolytic activity. Five isolates were positive for amylolytic activity. All of the Korean pepper isolates had a 32 kDa-protein unique to X. euvesicatoria and had the same band pattern of the rpoB gene as that of X. euvesicatoria and X. perforans as indicated by PCR-restriction fragment length polymorphism analysis. A phylogenetic tree of 16S rDNA sequences showed that all of the Korean pepper plant isolates fit into the same group as did all the reference strains of X. euvesicatoria and X. perforans. A phylogenetic tree of the nucleotide sequences of 3 housekeeping genes-gapA, gyrB, and lepA showed that all of the Korean pepper plant isolates fit into the same group as did all of the references strains of X. euvesicatoria. Based on the phenotypic and genotypic characteristics, we identified the pathogen as X. euvesicatoria. Neither X. vesicatoria, the known pathogen of pepper bacterial spot, nor X. perforans, the known pathogen of tomato plant, was isolated. Thus, we suggest that the pathogen causing bacterial spot disease of pepper plants in Korea is X. euvesicatoria.

• 동의생리병리학회지
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• 제20권5호
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• pp.1155-1158
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• 2006

The purpose of this study is to examine the cytotoxicity of species of Angelica (Angelicas Radix; the root of Angelica gigas Nakai, A. sinensis (Oliv.) Diels, and A. acutiloba Kitag.) on HepG2 cells. The water extracts of roots of Angelica gigas (WAG), A. sinensis (WAS), and A. acutiloba (WAA) were studied for HepG2 cell viability by a modified MTT assay in the concentrations of 5, 10, 50, 100, 250, 500 ug/ml for 24, 48, 72 h. WAG and WAS did not reduced the cell viability significantly. But WAA reduced the cell viability in the concentration of 500 ug/ml for 24 h (85.45%), 48 h (75.01%). In conclusion, WAG and WAS have not the significant cytotoxicity on HepG2 cells in the suitable dose.

• Journal of Nutrition and Health
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• 제32권5호
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• pp.570-576
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• 1999

This study was carried out to examine the effects of alcohol on serum lipid levels. Subjects of this study were 50 military officers living in Honsung, Korea, 25 of whom consumed alcohol on a regular basis and 25 of whom did not. All subjects were previously informed of the purpose of the study and the methods of food intake reporting and blood testing. The age distribution range of subjects was 30-49 years. Weight, height, and body mass index(BMI) were not significantly different between the two groups. Blood pressure was compared, and it was found that the drinker group had mean systolic(SBP) and diastolic blood pressures(DBP) 10mmHg higher than the non-drinker group. Nutrient intake levels of the two groups were not significantly different and were adequate with respect to the recommended dietary allowances. It was found that the drinker group ingested more calories, protein, fat and vitamn B1 than the non-drinker group, while the non-drinker group ingested more carbohydrate, calcium, iron, vitamin A, niacin and ascorbic acid. These differences were believed caused by increased caloric intake from alcohol and additional intake of meat served with drinks for the drinking group. Fourteen of the twenty-five drinkers(56%) had drank regularly for five to ten years. Most drinkers drank 2-3 times per week, with alcohol intake less than two bottles(720ml)of distilled liquor(Soju) at a time. Meat was often served with alcohol as reported by 72% of the drinker group, and was believed to contribute to raised intake of calories, protein and fat in this group. Serum lipid levels of both groups were classified as normal, but the components of total cholesterol, triglycerides were significantly higher in drinkers than non-drinkers. The correlation between triglyceride levels and alcohol intake at one sitting was shown to be significantly positive(r=0.57, p<0.01), so it was believed that triglyceride elevation was associated more with heavy drinking than light drinking. We can conclude that if alcohol is consumed with a normal diet, then serum cholesterol, triglyceride and total lipid levels are expected to increase.

• 한국학교ㆍ지역보건교육학회지
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• 제22권1호
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• pp.55-72
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• 2021

Objectives: The aim of this study was to construct and verify a structural equation model of advance directive intent among a Korean group in their middle-age. Methods: Data were collected between May 1 and 30, 2017, from 398 people. The endogenous and exogenous variables of the hypothetical model consisted of elderly parents' care burden, health status, attitude towards withdrawal of life sustaining treatment, advance directive efficacy, and advance directive intent. The collected data were analyzed using the SPSS/WIN 24.0 and Mplus 7.4. Results: The hypothetical model demonstrated a good fit: χ2=223.79(df=109, p<.001), CMIN/df=2.05 CFI=.96, TLI=.96, RMSEA=.05, SRMR=.06. Elderly parents' care burden and health status showed statistically significant direct effects with attitude toward withdrawal of life sustaining treatment(β=.17, p=.001; β=.21, p<.001) and advance directive efficacy(β=.11, p=.040; β=.19, p=.002), respectively. Attitude toward withdrawal of life sustaining treatment and advance directive efficacy showed statistically significant direct effects on advance directive intent(β=.15, p=.007; β=.48, p<.001). Elderly parents' care burden and health status had a significant indirect effect on advance directive intent through attitude toward withdrawal of life sustaining treatment(β=.01, p=.041; β=.05, p=.036) and advance directive efficacy(β=.06, p=.049; β=.16, p=.006), respectively. The variables accounted for 26.1% of advance directive intent of the Korean group in their middle-age. Conclusion: It is necessary to develop an advance directive education program based on variables affecting advance directive intent for individuals in their middle-age.

• Journal of Preventive Medicine and Public Health
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• 제55권5호
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• pp.485-491
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• 2022

Objectives: Tuberculosis (TB) is a major public health concern worldwide. Social contact patterns can affect the epidemiology and risk of airborne diseases such as TB. This study was designed to investigate the social contact patterns associated with TB. Methods: In this case-control study, groups of participants with and without TB were matched by age and sex. Participants reported the nature, location, frequency, and average duration of social contacts over 1 month. The duration and number of social and spatial contacts were compared between groups using the chi-square test and the t-test. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to quantify the relationship between social contact time and TB status. Data were analyzed using Stata version 11 statistical software. A p-value of <0.05 was considered to indicate statistical significance. Results: In this study, 80 patients with TB and 172 control participants were included, and a total of 3545 social contacts were registered. Social contact with family members (OR, 1.72; 95% CI, 1.10 to 2.40), contact with a person with TB (OR, 1.53; 95% CI, 1.16 to 2.01), and contact at the participant's home (OR, 1.42; 95% CI, 1.19 to 1.82) were significantly associated with TB status. Conclusions: The duration of long-term social contact, rather than the number of contacts, may be the main contact-related factor associated with TB transmission in this population. The focus of contact-tracing efforts should be on finding and treating both family members and long-term contacts in non-household settings.

• 대한지역사회영양학회지
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• 제28권4호
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• pp.317-328
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• 2023

Objectives: This study aimed to investigate the correlation between adolescents' dietary safety management competency, value recognition, efficacy, and competency of convergence using the dietary area (CUDA). Methods: Data were collected from 480 middle and high school students in Daegu, Gyeongbuk and Seoul, Gyeonggi using a self-administered five-point Likert scale questionnaire from May to July 2021. A questionnaire was used to investigate dietary safety management competency, awareness of convergence, recognition of the benefits, efficacy, and competency of CUDA. Results: We conducted factor, reliability, correlation, and regression analyses using SPSS 25. The average scores for each factor were: dietary significance (3.68); dietary safety management knowledge (3.34); food selection and cooking (3.72); nutrition management (3.38); weight management (3.28); risk dietary management (3.13); CUDA interest (2.98); convergence necessity (3.50); benefits in specialized areas (3.31); benefits in everyday life (3.48); efficacy of science and technology convergence (3.35); convergence efficacy with humanities, social science, and arts (3.31); and CUDA competency (3.41). The score for interest in CUDA was lower than that for the recognition of CUDA benefits. Significant positive correlations were observed between all factors except between risk dietary management and both nutrition and weight management (P < 0.01). Interest in CUDA and recognition of the need for convergence exhibited a positive and significant effect on all factors of the perception of CUDA benefits and efficacy. The subgroup factors of dietary safety management competency and the recognition of CUDA had a positive effect on the CUDA competency (P < 0.001, R²= 0.58). Conclusions: Strengthening dietary safety management competency and increasing the awareness of CUDA can enhance adolescents' convergence competency. Therefore, CUDA and targeted education must be actively promoted among adolescents.

• Journal of Veterinary Science
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• 제25권3호
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• pp.46.1-46.11
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• 2024

Importance: Rabies is a neglected tropical viral disease most often transmitted through the bite of an infected animal. Objective: This study assessed the level of knowledge, attitudes, and practices of the Shone Ttown community toward rabies. Methods: A survey-based cross-sectional study was conducted in Shone town, Ethiopia, from November 2022 to April 2023. Woreda was selected purposefully, while Kebeles and the study populations were selected by simple random sampling. Four hundred and sixteen respondents were interviewed using a semi-structured questionnaire. Results: All respondents had heard about rabies from different sources, with the majority hearing from informal sources (62%). Approximately 51.9%, 0.7%, and 47.4% of individuals were aware of saliva contact, rabid animal bites, and both as means of transmission, respectively. The survey showed that 64.4% of participants knew the 100% fatal nature of rabies once the clinical signs developed, and 35.6% did not. Approximately 51.4% of respondents agreed that killing stray dogs was an effective method for rabies prevention. In this study, 72.6% of the respondents had contact with pets, and 36.8% of the interviewees had vaccinated their dogs. Only the educational level (p = 0.03) was associated with knowledge of the transmission route. Age (p = 0.04) and educational level (p = 0.01) had a statistically significant association with knowledge of the risk of not vaccinating dogs. Conclusions and Relevance: A lack of formal education in the communities, low levels of education, and the majority of respondents acquiring their knowledge from unofficial sources are important contributors to the low levels of awareness.

• 한국미생물·생명공학회지
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• 제26권6호
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• pp.537-544
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• 1998

3-HV 몰분율이 증대된 P(3HB-3HV)의 고농도 생산을 위하여 재조합 phbC유전자를 모균주에 재도입시킨 형질전환 Alcaligenes eutrophus AER5의 배양공학적 연구를 수행하였다. 상기 형질전환균주를 전구물질인 valerate가 10.0g/L첨가된 최소배지에서 2단계 배양한 결과 P(3HB-3HV)내의 3-HV 몰분율이 52.2 mol%로 모균주 A. eutrophus H16의 30 mol%에 비해 현저히 증가하였다. 이와 같은 몰분율의 증가는 phbC 유전자의 산물인 PHB synthase의 증폭과 밀접한 관련이 있음을 확인하였다. 2단계 회분배양시 fructose의 보충첨가의 영향을 검토하였으며, 적정농도인 10.0g/L의 fructose를 valerate와 같이 첨가할 경우 총균체량, p(3HB-3HV)의 축적농도, 그리고 축적율은 현저히 증가한 반면, 3-HV 몰분율은 다소 감소하는 경향을 보였다. $Mg^{2+}$ 이온은 P(3HB-3HV) 축적 에 매우 민감한 영향을 미쳤으며, 적정 농도로 판단되는 0.1 g/L일 경우 P(3HB- 3HV) 축적농도는 6.1 g/L, 그리고 3-HV 몰분율은 71.3 %로 현저히 증가하였다. 또한 적정 pH 조절제의 선정을 위하여 NaOH, NaOH와 (NH$_4$)$_2$SO$_4$의 혼합액, 그리고 NH$_4$OH을 비교 검토하였다. 2단계 배양법의 번거로움을 극복하고저 1단계 간헐적 유가식배양법을 검토한 결과, 배양 72시간 후 총균체량 19.2g/L, P(3HB-3HV) 축적농도 10.4 g/L, 그리고 3-HV 몰분율 35 mol%로 2단계 회분 배양법보다 우수한 결과를 얻었다. 이는 형질전환균주를 이용한 3-HV 몰분율이 높은 P(3HB-3HV)의 고농도 생산을 위한 배양조건 확립을 위한 기초자료로 활용될 것이다.90배의 살충력을 보였다. SDS-PAGE와 Western blot 분석에서도 클론 pHLN2-72는 재조합 클론 pHLN2-80(-)보다 약간 높게 ICP가 생성이 되었었다. 이상의 결과는 과다발현에 Plac프로모터와 종결부위가 반드시 필요하며, -72 bp ICP 프로모터가 -80 bp 프로모터보다 과다발현률이 높았으며, ICP 유전자는 반드시 Plac프로모터의 전사 방향에 역방향으로 삽입이 되어야 하는 것으로 나타났다.사용함으로써 고품질의 전통주류 생산이 가능하다고 사료된다.noicacid, methylmalonic acid, 2-methyl-4-keto-pentan-2-ol 등과 지방산의 일종인 tetradecanoic acid, hexadecane, hept-adecanoic acid, octadecanoic acid 등이 확인되었다.er 7.6mm. Further rich management measure and investigation were recommended such as sapling protection, signboard construction, soil erosion controlling and regular monitoring within the community.ldom changed at level 1.will cause students to form the mathematical concepts correctly. 3. By visualizing the process of drawing the quadratic function graph, students understand the quadratic function graph structually. 4. Through the feedback, the recognition ability of the trigonometric function can be improved. 5. It is possible to change