• Title/Summary/Keyword: 01A29

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Taurine Transporter Activity in the Human Colon Carcinoma cell Line(HT-29) is Decreased during Cell Differentiation (인체 소장상피세포주(HT-29)의 분화단계에 따른 타우린수송체 활성의 변화)

  • 박태선
    • Journal of Nutrition and Health
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    • v.33 no.6
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    • pp.660-667
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    • 2000
  • Previous studies on the effect of age on intestinal taurine transport in animals have invariably shown a decline in the activity of the transport system with increasing age. In the present study changes in taurine transporter activity were observed during cell differentiation in the human colon carcinoma cell line HT-29 This cell line exhibits various enterocytic characteristics when differentiated and therefore has frequently been used to study the characteristcs and regulation of nutrient and drug absorption in the small intestine at the cellular level. Pre-treatment of the cells with $\beta$-alanine(10mM) reduced the taurine transport activity to 33% of the value for the control cells(p<0.05) which implies that taurine and $\beta$-alanine share a common $\beta$-amino acid transport system for their celluar uptake in the HI-29 was continued until 21 days post seeding. Kinetic studies of the taurine transporter were conducted in the HT-29 cell line with varying taurine concentration(5-60$\mu$M) in the uptake medium Both Vmax and the Michaelis-Menten constant(Km) of taurine transporter were decreased as differentiation of the HT-29 cell line was progressed ; Vmax of the taurine transporter in cells incubated for 4, 14 and 21 days post seeding was 2.79$\pm$3.4m 16.89$\pm$1.74, and 0.85$\pm$0.08 and 0.32$\pm$0.01nmol.mg protein-1 .30min-1 respectively(p<0.001) and Km was 42.3$\pm$3.4, 16.89$\pm$1.74, and 11.2$\pm$3.0$\mu$M respectively (p<0.01) These results indicate that the activity of sodium dependent active taurine transport system in the HT-29 cell line is decreased as confluent cells are differentiated. This phenomenon in cell culture system corresponds well with the earlier observation of lower intestinal taurine transport activity in suckling rats compared to that in adult animals although direct relationship of cell differentiation with in vivo aging process needs further verification.

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The change of muscle action potential and superficial temperature of spastic upper extremity in a patient with cerebral palsy by the water temperature (수온이 뇌성마비 환자의 상지 경련근의 표재 온도와 근 활동전위에 미치는 영향)

  • Seo Sam-Ki;Lee Jeong-Woo;Han Dong-Wook
    • The Journal of Korean Physical Therapy
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    • v.15 no.4
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    • pp.455-465
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    • 2003
  • The purpose of this study was to describe the alteration of muscle action potential of spastic upper extremity in a patient with cerebral palsy by the water temperature. We used seven patients with cerebral palsy. Participants classified according to each group in $29^{\circ}C,\;35^{\circ}C$ by the water temperature. All subjects participated 2 session, which at least 1 week between session. The test was measured continuously pre test, post-test by thermometer and surface EMG. The following results were obtained; 1. In changes of surface temperature, wrist flexor and extensor were significantly decreased in $29^{\circ}C$ (p<0.001) group but were significantly increased $35^{\circ}C$ group(p<0.01). 2. In changes of surface temperature, wrist flexor and extensor were significantly differenced between $29^{\circ}C$ and $35^{\circ}C$ group(p<0.001). 3. In changes of muscle action potential, wrist extension antagonist were significantly increased in $29^{\circ}C$ group(p<0.05). 4. In changes of muscle action potential, wrist flexion agonist were more significantly increased in $29^{\circ}C$ group(p<0.01). 5. In changes of muscle action potential, wrist extension antagonist were significantly differenced between $29^{\circ}C$ and $35^{\circ}C$ group(p<0.05). These results lead us to the conclusion that changes of muscle action potential of spastic upper extremity in a patient with cerebral palsy were influenced by the water temperature. Therefore, a further direction of this study will be to provide more evidence that a moderate water temperature have an effect on muscle tone in a patient with cerebral palsy.

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Study of Factors Leading to Seasonality of Reproduction in Dairy Buffaloes. II. Non-Nutritional Factors

  • Qureshi, M.S.;Habib, G.;Samad, H.A.;Lodhi, L.A.;Usmani, R.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.7
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    • pp.1025-1030
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    • 1999
  • Fifth one Nili-Ravi dairy buffaloes in their last two months of gestation, were selected in NWFP Pakistan. Rectal palpation was carried out fortnightly, until the occurrence of first estrus. Ovulation was confirmed per rectum and milk progesterone levels (MPL). Body condition score (BCS)was recorded weekly. Milk samples were collected weekly for MPL and blood samples fortnightly for metabolites analysis. Milk yield was recorded and samples were collected for milk fat. The buffaloes calving during the normal breeding season (NBS, August to January)had short (p<0.01) postpartum estrus interval of $55.95{\pm}4.90$ days versus $91.15{\pm}11.61 $ days in the buffaloes calving during the low breeding season (LBS, February to July). MPL in the LBS calvers remained lower than NBS calves (p<0.01). The incidence of silent ovulation was higher during LBS as compared to NBS (70.6% versus 29.4%). MPL showed a pattern opposite to atmospheric temperature. In NBS calvers serum glucose levels were higher (p<0.01) and magnesium levels were lower (p<0.01) than LBS calvers. Higher serum urea was found in summer and spring than that autumn and winter (p<0.05). The reproductive performance in buffaloes calving in the LBS coincided with a low BCS (p<0.01). Fat corrected milk production (FCM) was higher in NBS than LBS (p<0.01) calvers.

Effects of Dietary Education on Low-sodium Diet Adaptation (식생활교육이 저나트륨식 적응에 미치는 영향)

  • Kim, Hae Young;Kim, Juhyeon
    • Journal of the Korean Society of Food Culture
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    • v.29 no.2
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    • pp.212-221
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    • 2014
  • Korean style DASH (Dietary Approaches to Stop Hypertension) and a dietary education program for sodium reduction were developed. Reduced sodium diets (15 and 30% reductions) were developed from general diets for 3 consecutive weeks from Monday through Saturday. Subjects (19 total) were classified into two groups according to dietary education. Experimental period was from June 24 to July 23, 2012. Total sum of adaptation scores for low sodium diets significantly increased in the group that underwent dietary education compared to that without (p<0.05). After the experiment, both groups showed significantly increased values in terms of food group balance, sodium-related nutrition knowledge, attitude, and practice by paired t-test. Especially, group that underwent dietary education showed significantly higher values for attitudes by ANCOVA pre-test as a variation (p<0.01). For the results of the nutrient intake survey, group that underwent dietary education showed significantly increased values for dietary fiber (p<0.01), vitamin A (p<0.001), vitamin K (p<0.001), vitamin C (p<0.01), Folic acid (p<0.001), vitamin B12 (p<0.01), calcium (p<0.01), iron (p<0.05), and zinc (p<0.05) and significantly decreased values for sodium (p<0.05) and chloride (p<0.005). Subjects adapted to reduced sodium diets showed apparent improvements in sodium-related knowledge, attitude, practice and intake of nutrient, and these improvements were even higher in the group that underwent dietary education compared to that without. Thus, adaptation to low sodium diet combined with dietary education can improve dietary habits.

A Study on the burnout of firefighters (소방대원의 소진(Burnout)에 관한 연구)

  • Lee, In-Soo;Choi, Eun-Sook
    • The Korean Journal of Emergency Medical Services
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    • v.4 no.1
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    • pp.47-55
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    • 2000
  • The study was conducted to investigate the burnout of firefighters. The subjects were 223 firefighters in C-Do. The instruments used for this study were a survey of general characteristics(10 items), burnout(23 items). Analysis of data was done by use of mean percentage, t-test, ANOVA, stepwise multiple regression with SAS program. The results of this study are as follows; 1. Burnout according to general characteristics were showed significant difference in job-aptitude(F=6.30, P<.001), health status(F=5.92, P<.001), education(t=6.09, P<.01), working place rotation(t=2.59, P<.01), call number/week(F=3.28, P<.01), job-burden(F=4.45, P<.01), paper-job burden(F=3.87, P<.05). 2. Category score of burnout were showed the higher score in the lowed personal accomplishment(3.29). 3. Category score of burnout according to aptitude were showed difference each other. 4. Predicting factor of burnout were paper-job burden, health status, job-burden(20.99%).

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Factors Affecting Dysmenorrhea among Adolescents (청소년의 월경곤란증과 영향 요인)

  • Jung Hyang-Mi;Kim Yi-Soon
    • Child Health Nursing Research
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    • v.10 no.2
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    • pp.196-204
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    • 2004
  • Purpose: This research was to determine the characteristics of dysmenorrhea among girls, identify the factors affecting dysmenorrhea. Method: The study subjects were 1653 high school student in Busan Metropolitan city. The data were collected by a self-administered questionnaire between November 1 and November 30, 2002. Descriptive statistics, t-test, one-way ANOVA with Scheffe' test, and stepwise multiple regression analysis in SPSS program were used for data analysis. Result: Students suffering from slight dysmenorrhea made up the majority 52.9%, whereas those with serious cases represented 29.8%. Regarding the measures taken to cope with dysmenorrhea, most of the respondents took analgesics 52.8%. A high points of dysmenorrhea was associated with irregular meal habits(p<0.01), unbalanced diet(p<0.05), stress(p<0.01), and health awareness(p<0.01), first menstruation before they reached 13 (p<0.01), heavy bleeding(p<0.01), family history(p<0.01). Factors affecting the dysmennorrhea of the respondents included the extent of bleeding, family history, health awareness, age during the first menstruation, and degree of stress, accounting for 13.1% of the total variance. Conclusion: To manage dysmenorrhea among girls, the results of the research indicate that students need to have proper awareness of health and manage stress through health education. Likewise, they need to be given positive follow-up management through a holistic nursing approach.

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CONVERGENCE THEOREMS FOR GENERALIZED α-NONEXPANSIVE MAPPINGS IN UNIFORMLY HYPERBOLIC SPACES

  • J. K. Kim;Samir Dashputre;Padmavati;Rashmi Verma
    • Nonlinear Functional Analysis and Applications
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    • v.29 no.1
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    • pp.1-14
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    • 2024
  • In this paper, we establish strong and ∆-convergence theorems for new iteration process namely S-R iteration process for a generalized α-nonexpansive mappings in a uniformly convex hyperbolic space and also we show that our iteration process is faster than other iteration processes appear in the current literature's. Our results extend the corresponding results of Ullah et al. [5], Imdad et al. [16] in the setting of uniformly convex hyperbolic spaces and many more in this direction.

Depression, Health-Related Habits, Eating Habits, and Nutrient Intake of Male Youth Before and After the Outbreak of the COVID-19 Pandemic -Analysis of the 2018 and 2020 Korea National Health and Nutrition Examination Survey- (코로나19 전후 남자청년의 우울, 건강 관련 습관, 식습관 및 영양소 섭취량의 변화 -2018년, 2020년 국민건강영양조사 자료 분석-)

  • Kyung-Ae Park
    • Journal of the Korean Dietetic Association
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    • v.29 no.4
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    • pp.211-229
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    • 2023
  • This study analyzed the differences in the general characteristics, mental and health-related factors, eating habits, and nutrient intake of Korean male youths before and after the outbreak of the coronavirus disease 2019 (COVID-19). The data of a total of 1,231 subjects, from among the participants in the 2018 and 2020 Korea National Health and Nutrition Examination Survey (KNHANES), were analyzed. The 2020 group had fewer married members than the 2018 group. The subjects in the 2020 group weighed more (P<0.05), had a higher waist circumference (P<0.01), and showed greater weight gain (P<0.05) than the 2018 group subjects. The aerobic physical activity of the 2020 group was lower (P<0.01) compared to the 2018 group. The members of the 2020 group had higher depression scores (P<0.01), suicidal tendencies (P<0.01), and need for counseling for mental problems (P<0.05) than those of the 2018 group. The prevalence of high fasting blood sugar levels and diabetes was higher in the 2020 group compared to the 2018 group. The frequency of breakfast (P<0.01) and dining out (P<0.001) and the proportion of breakfast (P<0.01) and dinner (P<0.05) taken with the family or non-family members was lower in the 2020 group than in the 2018 group. The rate of dietary supplement intake of the 2020 group was higher than that of the 2018 group (P<0.01). There were significant differences in the protein (P<0.05), riboflavin (P<0.01), and carbohydrate (P<0.01) intakes between the two groups. These results suggest that male youths had a higher waist circumference and high levels of depression, and showed a decrease in frequency of breakfast intake and dining out after the out break COVID-19 pandemic. Therefore, nutrition and health promotion programs are urgently needed to improve mental health, other health-related factors and nutritional status of male adults that are associated with the COVID-19 pandemic.

Relationships between Nursing Practice Stress and Stress Coping of Nursing Students in Korea (한국간호대학생의 임상실습 스트레스와 스트레스 대처)

  • Kim, Jihyun
    • Journal of Digital Convergence
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    • v.12 no.2
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    • pp.409-415
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    • 2014
  • This study was done to identify the relationships between nursing practice stress and stress coping for the nursing students in Korea. Data were collected at 2 universities located in C city in Korea. Participants were 160 nursing students. Data were analyzed by SPSS 18.0 using descriptive statistics and Pearson's correlation coefficient. Nursing practice stress was $2.72{\pm}6.2$. Stress coping was $2.60{\pm}0.37$. Stress was correlated with wishful thinking coping strategy when doing orientation (r=0.32, p<0.01), conference(r=0.52, p<0.01), homework(r=0.29, p<0.01). Stress for practice exam was correlated with problem focused coping strategy(r=0.31, p<0.05). Stress was correlated with positive view coping strategy when face nurse(r=0.31, p<0.01) or interpersonal relations(r=0.35, p<0.01). Stress related to practice environment and class was correlated with indifference (r=0.26, p<0.01) and tension relieve strategy (r=0.28, p<0.01). There's no stress when they meet or serve direct care to patients. Students managed the stress by showing active coping pattern. It is considered that the experience gave students an excellent capacity to cope with stress. Since the degree of stress and coping pattern can differ from adaptability of situation, a research of change in clinical training stress by seasonal clinical training and coping patter is proposed.