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Reduction of Highly Concentrated Perchlorate in Aqueous Medium by Newly Isolated Bacterial Strains Rhodococcus sp. YSPW01 and YSPW02 (신균주 Rhodococcus sp. YSPW01과 YSPW02를 이용한 수중 내 고농도 Perchlorate 환원 특성 연구)

  • Lee, Sang-Hoon;Hwang, Jae-Hoon;Akhil, Kabra;Lee, Dae Sung;Jeon, Byong-Hun
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.5
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    • pp.352-358
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    • 2014
  • The feasibility of perchlorate reducing-bacteria isolated from the sludge of an anaerobic digester was determined using ammonium perchlorate in aqueous medium. Growth kinetics of the two perchlorate reducing bacteria including Rhodococcus sp. YSPW01 and YSPW02 were investigated using acetate as the electron donor in batch experiment. The growth of YSPW01 and YSPW02 reached a steady-state at 26 and 9 h, respectively. The initial perchlorate concentration was completely reduced within 8 and 7 h by YSPW01 and YSPW02, respectively. The reduction rates were 2.1 and $15mg\;L^{-1}h^{-1}$ for YSPW01, and 3.2 and $15.5mg\;L^{-1}h^{-1}$ for YSPW02, at 1:1 and 5:1 ratios of acetate:perchlorate (w:w), respectively. In this study, the bacteria Rhodococcus sp. YSPW01 and YSPW02 demonstrated a potential for the perchlorate reduction, which could be further investigated for development of an efficient strategy to treat the perchlorate contaminated waters.

Antioxidant Effects of Gagam-jangwon-hwan(jiajianzhuangyuanwan) on Hydrogen Peroxide-Induced Oxidative Stress in PC 12 Cell Lines ($H_2O_2$로 유도된 산화적 스트레스에 대한 장원환가감방(壯元丸加減方)의 PC 12 cell 에서의 항산화 효과)

  • Park, Yong-Hoon;Son, Il-Hong;Lee, Sang-Won;Lim, Jung-Hyun;Kim, Tae-Heon;Lyu, Yeoung-Su;Kang, Hyung-Won
    • Journal of Oriental Neuropsychiatry
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    • v.20 no.2
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    • pp.19-29
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    • 2009
  • Objectives : Antioxidant effects of Gagam-jangwonhwan(LMK01 and 02) water extract against $H_2O_2$-induced oxidative damage and cell death were investigated in rat pheochromocytoma line PC 12. Methods : The cells were treated with LMK01 and 02 water extract and $H_2O_2$, oxidative damage-inducing materials for 24 h. The cellular viability was assessed by WST-1 assay, oxidative damages of the cells by 8-OHdG quantitation, apoptosis by Hoechst 33342 staining assay and activity of antioxidant enzymes by catalase and glutathione peroxidase assay. Results : 1. LMK01 and LMK02 water extracts improved significantly cell viability in $H_2O_2$-treated groups than $H_2O_2$-alone treated cells 2, LMK02 suppressed significantly oxidative damage in $H_2O_2$-treated groups than $H_2O_2$-alone treated cells but LMK01 didn't. Meanwhile, difference of oxidative damages in conditions treated with LMK01 or LMK02 was not significant, 3. The $H_2O_2$ induced-apoptosis in PC 12 cell lines was inhibited effectively by LMK01 and LMK02, and especially the features of apoptosis were obviously reduced in LMK02-treated cells. 4. LMK01 and LMK02 increased significantly activities of both catalase and glutathione peroxidase than those of $H_2O_2$-alone treated group and moreover, LMK02 showed significantly higher activities than those of LMK01. Conclusions : As shown, LMK01 and LMK02 suppressed $H_2O_2$-induced oxidative damage and cell death in PC 12 cell effectively. And they increased activity of major antioxidant enzymes in PC 12 cell line. Therefore, this study suggests the possibility of clinical usage over oxidative stress-induced neurodegenerative disease such as Alzheimer's disease.

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A Case Study for Efficient Blended Learning Management (효율적인 혼합형 학습 운영을 위한 사례연구)

  • Kwon, Oh-Young
    • The Journal of Korean Institute for Practical Engineering Education
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    • v.2 no.1
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    • pp.52-57
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    • 2010
  • Using the Operating Systems course that is offered by online, a blended learning mixed up with face-to-face lecture and e-learning for O.S. course has been carried out. In order to find a efficient management way of the blended learning, we build up two groups: one group named 01 takes a class which consists of two hours face-to-face lecture and one hour online study per week and the other group named 02 takes a class which consists of two hours online study and one hour face-to-face lecture. According to the result of a mid-term examination, the Cohen's d between two groups is 0.165. It means the small effect size. The 01 group has higer average and smaller variance than 02 group. However, 02 group has more students who earn high score than 01 group. In conclusion, if students can well carry out the self-regulated learning, then the blended learning mixed up with 02 group style is suitable. Otherwise, face-to-face lecture or the blended learning like 01 group style is suitable.

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Estimation of heritabilities and additive genetic correlations for reproduction traits in swine: insights for tropical commercial production systems using multiple trait animal models

  • Udomsak Noppibool;Thanathip Suwanasopee;Mauricio A. Elzo;Skorn Koonawootrittriron
    • Animal Bioscience
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    • v.36 no.12
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    • pp.1785-1795
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    • 2023
  • Objective: This study was to estimate heritabilities, additive genetic correlations, and phenotypic correlations between number of piglets born alive (NBA), litter birth weight (LTBW), number of piglets weaned (NPW) and litter weaning weight (LTWW) in different parities of Landrace (L), Yorkshire (Y), Landrace×Yorkshire (LY), and Yorkshire×Landrace (YL) sows in a commercial swine operation in Northern Thailand. Methods: Two models were utilized, a single trait repeatability model (RM) and a multiple trait animal model (MTM). The RM assumed reproductive records from different parities to be repeated values of the same trait, whereas the MTM assumed these records to be different traits. The two models accounted for the fixed effects of farrowing year-season, genetic group of the sow, heterosis, and age at first farrowing, and the random effects of sow, boar, and residual. Results: Heritability estimates from RM were 0.02±0.01 for NBA, 0.10±0.01 for LTBW, 0.04±0.01 for NPW, and 0.11±0.01 for LTWW. Heritability estimates from MTM fluctuated across parities, ranging from 0.04±0.01 in parity 2 to 0.09±0.02 in parity 4 for NBA, 0.07±0.02 in parity 2 to 0.16±0.02 in parity 3 for LTBW, 0.04±0.02 in parity 4 to 0.08±0.01 in parity 1 for NPW, and 0.16±0.02 in parity 1 to 0.20±0.02 in parity 2 for LTWW. Additive genetic correlation estimates from MTM were also variable, ranging from 0.29±0.24 between NBA in parity 1 and NBA in parity 2 to 0.99±0.05 between LTWW in parity 3 and LTWW in parity 4. Conclusion: The findings of this study highlight the advantage of using MTM for the genetic improvement of reproductive traits in swine and contribute to the development of sustainable swine breeding programs in Thailand.

A Study on Textile Design for Apparel Using CAD - Focusing on '01/02 A/W Fashion Trend - (CAD를 이용(利用)한 의류용(衣類用) 텍스타일 디자인 연구(硏究) - '01/02 A/W 유행경향(流行傾向)을 중심(中心)으로 -)

  • Shin, Kyeong-Seob
    • Journal of Fashion Business
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    • v.4 no.4
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    • pp.41-54
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    • 2000
  • The purpose of this study was to investigate various expressions in textile design using CAD and present print design of '01/02 A/W. Four steps were taken in order to achieve this purpose. The first step was to investigate prior research and to analyze textile design methods using CAD. The second step was to study '01/02 A/W fashion trend, especially textile trend. The third step was to design the print design according to textile trend and apply the designed print pattern to real model. The research results are as follows: 1. Textile design is a very important field to help making fashion industry more informative and valuable. 2. Using CAD system in textile design will be very useful and urgent in saving working time and exercising design works. 3. In '01/02 A/W hound's tooth, zigzag, herringbone will be prevalent bold geometric patterns while tartan checks, multi color stripes will be popular too. 4. Textile designs using flower, stripe, checks, dot, abstract pattern were applied to real model, the images of patterns design are as follow, flower pattern-romantic stripe-avantgarde check-cute dotclassic abstract motive-intelligent.

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2002년도 축산물등급판정 결산

  • 박종운
    • KAPE Magazine
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    • no.77
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    • pp.4-9
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    • 2003
  • [ $\blacksquare$ ] 등급판정 현황 - 소도체는 사육두수의 감소로 인해 전년대비 13.3$\%$ 감소하였으나, 등급판정대상 두수 전량(100$\%$) 판정 - 돼지도체는 사육두수의 증가와 등급거래지역의 확대로 전년대비 8.4$\%$가 증가하였고, 도축두수대비 98$\%$ 판정 - 계란등급판정 시범사업은 집하장 3개소가 늘어나 모두 4개소에서 실시 $\blacksquare$ 고급육 출하 - 한우 1등급이상 : (`01) 29.9$\%$ $\rightarrow$ (`02) 35.2$\%$ (5.3$\%$포인트 증가) - 돼지 A$\cdot$B등급 : (`01) 67.3$\%$ $\rightarrow$ (`02) 68.5$\%$ (1.2$\%$포인트 증가) $\blacksquare$ 한우 성별 출하 - 암소 : (`01) 52.8$\%$ $\rightarrow$ (`02) 48.3$\%$ (4.5$\%$포인트 감소) - 거세율 : (`01) 20.6$\%$ $\rightarrow$ (`02) 38.2$\%$ (17.6$\%$포인트 증가)

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Emulsion Stability of Cosmetic Facial Cream O/W Emulsions Prepared by Brij Type Non-ionic Emulsifie (Brij계 비이온성 혼합유화제를 이용하여 제조된 화장용크림 O/W 유화액의 유화안정성)

  • Park, Bo Ra;Lee, Seung Min;Choi, Junho;Lee, Seung Bum
    • Applied Chemistry for Engineering
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    • v.29 no.4
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    • pp.440-445
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    • 2018
  • In this study, the stability of O/W cosmetic facial cream emulsions according to HLB values was evaluated by mixing nonionic surfactants, such as Brij 78&72 and Brij 98&92. Brij 78&72 (steareth-20&steareth-2, EMS-01), saturated fatty acid, and Brij 98&92 (oleth-20&oleth-2, EMS-02), unsaturated fatty acid, were used as mixed surfactants. The stability of the O/W emulsion was evaluated by using the emulsion viscosity, particle size, particle size distribution, and zeta-potential. The viscosity of the emulsion increased with the increase of time for EMS-01 while that of EMS-02 decreased with the increase of HLB value. The particle size of both EMS-01 and EMS-02 increased with time. The emulsifier with a HLB value of 10.8, which is the most similar to the required HLB value of mineral oil, 10.5, had the smallest particle size and highest density and also showed the highest emulsion stability. The zeta-potential of both emulsions tended to increase with the HLB value. No significant changes were observed in emulsions of the HLB value of 10.8 or more. The saturated fatty acid system, EMS-01, exhibited a higher zeta-potential value than that of the unsaturated fatty acid EMS-02 and also was superior in the stability.

Biological Control of Soft Rot on Chinese Cabbage Using Beneficial Bacterial Agents in Greenhouse and Field (유용세균(Beneficial Bacterial Agents)을 이용한 배추 무름병의 생물적 방제)

  • Shrestha, Anupama;Kim, Eun-Chang;Lim, Chuen-Keun;Cho, Sae-Youll;Hur, Jang-Hyun;Park, Duck-Hwan
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.325-331
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    • 2009
  • Three beneficial bacterial agents, Lactobacillus strain KLF01, Lactococcus strain KLC02 and Paenibacillus strain KPB3 were showed clear zone against plated Pectobacterium carotovorum subsp. carotovorum (Pcc) soft rot pathogen. In greenhouse test, bio-control efficacy was more significantly effective in the treatments by KLC02 and KPB3 as 64%, 50%, 56% and 66%, 57%, 58% according to date of evaluation, respectively. In case of KLF01 control effect was relatively lower than treatments of KLC02 and KPB3 but its efficacy was still significantly observed when compared to control (only water treatment). Furthermore, KLF01, KLC02 and KPB3 showed 55%, 60% and 62% bio-control efficacy, respectively in field test from early March to late July of 2009. Thus, we suggest that these strains can be useful as bio-control agents against soft rot caused by Pcc.

Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater

  • Chen, Colin S.;Tien, Chien-Jun;Zhan, Kai-Van
    • Journal of Soil and Groundwater Environment
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    • v.19 no.5
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    • pp.9-17
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    • 2014
  • This paper reported the use of real-time polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and the culture-based method in the intrinsic bioremediation study at a petroleum contaminated site. The study showed that phenol hydroxylase gene was detected in groundwater contaminated with benzene, toluene, ethylbenzene, xylene isomers (BTEX) and methyl tert-butyl ether (MTBE). This indicated that intrinsic bioremediation occurred at the site. DGGE analyses revealed that the petroleum-hydrocarbon plume caused the variation in microbial communities. MTBE degraders including Pseudomonas sp. NKNU01, Bacillus sp. NKNU01, Klebsiella sp. NKNU01, Enterobacter sp. NKNU01, and Enterobacter sp. NKNU02 were isolated from the contaminated groundwater using the cultured-based method. Among these five strains, Enterobacter sp. NKNU02 is the most effective stain at degrading MTBE without the addition of pentane. The MTBE biodegradation experiment indicated that the isolated bacteria were affected by propane. Biodegradation of MTBE was decreased but not totally inhibited in the mixtures of BTEX. Enterobacter sp. NKNU02 degraded about 60% of MTBE in the bioreactor study. Tert-butyl alcohol (TBA), acetic acid, 2-propanol, and propenoic acid were detected using gas chromatography/mass spectrometry during MTBE degraded by the rest cells of Enterobacter sp. NKNU02. The effectiveness of bioremediation of MTBE was assessed for potential field-scale application.

A Study on the Magnetic Properties of Polycrystailine${(Fe,In,Eu)}_2O_3$ (다결정${(Fe,In,Eu)}_2O_3$계의 자기적 성질에 관한 연구)

  • 김정기;서정철;한은주;홍양기
    • Journal of the Korean Magnetics Society
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    • v.1 no.1
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    • pp.1-5
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    • 1991
  • The magnetic properties of the polycrystalline ${(Fe_{2}O_{3})}_{1-x-y}{(In_{2}O_{3})}_{x}{(Eu_{2}O_{3})}_{y}$(x=0.01, y=0.02과 x=0.02, y=0.03) have been studied by the methods of X-ray diffraction, $M\"{o}ssbauer$ effect, and magnetic hysteresis measurement. The X-ray diffraction patterns show that the samples have a same crystal structure as $\alpha-Fe_{2}O_{3}$. From the analysis of the temperature dependence of the quadrupole splitting and average half-width, it is found that the Morin transition occurs in the sample of x=0.01 and y=0.02 and the spin angle defined as the angle between the [111] crystal axis and antiferromagnetic vector, changes from about $35^{\circ}$ to the (111) plane as increasing the temperature in the sample of x=0.02 and y=O.03. The temperature dependence of magnetic hyperfine field is analyzed by using the spin-wave theory. The isomer shift values at room temperature are found to be given by about 0.35mm/s for the samples which means that the Fe ions belong to $3^{+}ion$. The temperature dependence of isomer shift was analyzed by using the Debye model.

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