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Quantitative Analysis of Bioactive Compounds in the Fruits of Crataegus pinnatifida by High-Performance Liquid Chromatography

  • Bae, Yoon-Ho;Cuong, To Dao;Lee, Jae-Hyun;Woo, Mi-Hee;Choi, Jae-Sue;Min, Byung-Sun
    • Natural Product Sciences
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    • 제18권2호
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    • pp.83-88
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    • 2012
  • In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical markers of C. pinnatifida. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254nm. All three calibration curves showed good linearity ($R^2$ > 0.998). The recoveries of three marker compounds were in the range of 94.87~111.52 %. The contents of chlorogenic acid (1), rutin (2), and hyperin (3) of the fruits of C. pinnatifida collected from 23 district markets in Korea, Japan, and China were 0.16~0.65 mg/g, 0.07~1.24 mg/g, and 0.03~0.62 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of the fruits of C. pinnatifida.

Genetic diversity and population structure of rice accessions from South Asia using SSR markers

  • Cui, Hao;Moe, Kyaw Thu;Chung, Jong-Wook;Cho, Young-Il;Lee, Gi-An;Park, Yong-Jin
    • 한국육종학회지
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    • 제42권1호
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    • pp.11-22
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    • 2010
  • The population structure of a domesticated species is influenced by the natural history of the populations of its pre-domesticated ancestors, as well as by the breeding system and complexity of breeding practices implemented by humans. In the genetic and population structure analysis of 122 South Asia collections using 29 simple sequence repeat (SSR) markers, 362 alleles were detected, with an average of 12.5 per locus. The average expected heterozygosity and polymorphism information content (PIC) for each SSR locus were 0.74 and 0.72,respectively. The model-based structure analysis revealed the presence of three clusters with the 91.8% (shared > 75%) membership, with 8.2% showing admixture. The genetic distances of Clusters 1-3 were 0.55, 0.56, and 0.68, respectively. Polymorphic information content followed the same trend (Cluster 3 had the highest value and Cluster 1 had smallest value), with genetic distances for each cluster of 0.52, 0.52, and 0.65, respectively. This result could be used for supporting rice breeding programs in South Asia countries.

단순 연소법으로 합성한 LiNi0.5Mn0.3Co0.2O2 양극 활물질의 구조 분석 및 전기화학적 특성 연구 (Structure and Electrochemical Characterization of LiNi0.5Mn0.3Co0.2O2 as the Cathode Material Synthesized by Simple-combustion Method)

  • 조성우;주정훈;류성현;류광선
    • 전기화학회지
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    • 제13권4호
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    • pp.264-269
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    • 2010
  • $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$의 리튬이온 이차전지 양극 물질로의 특성을 연구하기 위해서 단순 연소합성법을 이용하여 합성했다. 합성된 물질의 구조적 특징을 분석하기 위하여 X-선 회절분석(XRD)과 주사전자현미경 (FE-SEM)을 측정하였다. X-선 회절분석을 통하여 합성된 $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$시료가 육방정계 층상구조가 형성된 것을 확인하였다. FE-SEM을 통해 측정한 결과 $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$ 입자는 일정한 형태를 가지지 않았으며 크기는 대략 100~300 nm의 크기임을 확인할 수 있었다. 그리고 전기화학적 특성을 측정하기 위하여 충 방전 용량 측정과 CV(Cyclic Voltammetry)를 측정하였다. 2.8 V에서 4.3 V까지 충 방전 용량을 측정한 결과 ~162 mAh/g의 초기 방전 용량을 가졌다.

Serum interleukin-1beta and tumor necrosis factor-alpha in febrile seizures: is there a link?

  • Mahyar, Abolfazl;Ayazi, Parviz;Orangpour, Reza;Daneshi-Kohan, Mohammad Mahdi;Sarokhani, Mohammad Reza;Javadi, Amir;Habibi, Morteza;Talebi-Bakhshayesh, Mousa
    • Clinical and Experimental Pediatrics
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    • 제57권10호
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    • pp.440-444
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    • 2014
  • Purpose: Febrile seizures are induced by fever and are the most common type of seizures in children. Although numerous studies have been performed on febrile seizures, their pathophysiology remains unclear. Recent studies have shown that cytokines may play a role in the pathogenesis of febrile seizures. The present study was conducted to identify potential links between serum interleukin-1beta (IL-$1{\beta}$), tumor necrosis factor-alpha (TNF-${\alpha}$), and febrile seizures. Methods: Ninety-two patients with simple or complex febrile seizures (46 patients per seizure type), and 46 controls with comparable age, sex, and severity of temperature were enrolled. Results: The median concentrations of serum IL-$1{\beta}$ in the simple, complex febrile seizure, and control groups were 0.05, 0.1, and 0.67 pg/mL, respectively (P=0.001). Moreover, the median concentrations of TNF-${\alpha}$ in the simple, complex febrile seizure, and control groups were 2.5, 1, and 61.5 pg/mL, respectively (P=0.001). Furthermore, there were significant differences between the case groups in serum IL-$1{\beta}$ and TNF-${\alpha}$ levels (P<0.05). Conclusion: Unlike previous studies, our study does not support the hypothesis that increased IL-$1{\beta}$ and TNF-${\alpha}$ production is involved in the pathogenesis of febrile seizures.

Epidemiologic Analysis of Burns in Military Hospital

  • Choi, Jangkyu;Park, Sejin;Kim, Hyun Chul
    • Journal of Trauma and Injury
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    • 제30권4호
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    • pp.145-157
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    • 2017
  • Purpose: We accessed epidemioloy of 908 acute burns (7 years) in the military, of injuries and propose proper educational programs to suit community. Methods: We surveyed burn demographics, circumstances of injuries, size, result of treatment. Results: The mean age was 20.6 years. The flame burns (FB) (325, 35.8%) were most common, followed scald (SB) (305, 33.6%), contact (CB) (219, 24.1%), electric (EB) (45, 5.0%) and chemical burns (ChB) (14, 1.5%). The more occurred during winter (29.7%). SB had mean 3.9% total body surface area (TBSA). The 251 (82.3%) had superficial burns by spillage of hot water/food on lower limbs (45.6%), feet (33.8%) in summer (34.8%), treated with simple dressing (92.8%). Morbidity rate was 5.6%; post traumatic stress disease (PTSD) (0.7%). FB had large wound (9.3% TBSA). The 209 (64.3%) had superficial burns by ignition to flammable oils (31.7%) and bomb powders (29.2%) on head/neck (60.3%), hands (58.6%) in summer (31.7%), autumn (30.2%). They underwent simple dressing (83.4%) and skin graft (16.0%). Morbidity rate was 18.8%; PTSD (10.5%), inhalation injuries (4.0%), corneal injury (3.7%), amputations (0.9%), and mortality rate (1.2%). CB had small (1.1% TBSA), deep burns (78.5%) by hotpack (80.4%) on lower limbs (80.4%). The more (59.8%) underwent skin graft. EB had 6.8% TBSA. The 29 (64.4%) had superficial burns by touching to high tension cable (71.1%) on hand (71.1%), upper limbs (24.4%) in autumn (46.8%). They underwent simple dressing (71.1%) and skin graft (24.4%). They showed high morbidity rate (40.0%); loss of consciousness (13.3%), nerve injuries (11.1%), neuropathy (8.9%), amputations (2.2%), and mortality rate (2.2%). Conclusions: The cook should wear apron over the boots during work. The lighter or smoking should be strictly prohibited during work with flammable liquids or bomb powders. Don't directly apply hotpack to skin for a long time. Use insulating glove during electric work. Keep to the basic can prevent severe injury and proper education is important.

하천환경의 생물학적 평가를 위한 간이저서동물지수(SBMI)의 개발 (Development of Simple Benthic Macroinvertebrates Index (SBMI) for Biological Assessment on Stream Environment)

  • 공동수;민정기;노성유
    • 한국물환경학회지
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    • 제34권5호
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    • pp.514-536
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    • 2018
  • GPI (Group Pollution Index) using 29 indicator groups of Korean benthic macroinvertebrates was proposed in 1992, a higher category taxa-level index developed for rapid field assessment of organic water pollution. This study was performed to revise the assessment scheme of GPI based on taxonomic performance and ecological information accumulated since 1992. The original GPI was renamed SBMI (Simple Benthic Macroinvertebrates Index), and SBMI was based on saprobic valency of 26 indicator groups composed of higher category taxa (mainly family ~ phylum) excluding some genus or species-level taxa. SBMI revealed highly significant correlation with concentration of 5-day biochemical oxygen demand ($BOD_5$) (correlation coefficient r = 0.78, n = 569 sites), total suspended solids (r = 0.69), and total phosphorus (r = 0.77). Also, SBMI revealed strong correlation with Shannon-Weaver's species diversity (r = -0.85), Margalef's species richness (r = -0.85), and McNaughton's dominance (r = 0.83). Determination coefficient of SBMI to concentration of water quality items and values of community indices such as species diversity was 3 ~ 8 % and approximately 11 ~ 14 % higher than that of GPI, respectively. Correlation between SBMI and water quality factors or community indices such as species diversity did not reveal much difference compared to that of species-level indices, such as BMI (Benthic Macroinvertebrates Index) and ESB (Ecological Score of Benthic Macroinvertebrates). SMBI is a simple-qualitative index with higher category taxa easily identified, and is applicable for rapid field assessment of water environment impairment.

당뇨환자의 영양지식수준에 따른 자가관리 현황 및 웹베이스 영양관리 프로그램 개발에 관한 요구도 조사 (Needs Assessment for Web-based Self-management Program by the Nutrition Knowledge Levels of Diabetic Patients)

  • 안윤;배제헌;윤정은;김희선
    • 대한지역사회영양학회지
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    • 제16권1호
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    • pp.155-168
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    • 2011
  • This study was conducted to assess needs of self-management nutrition program for diabetic patients. The survey was conducted among 100 diabetic patients, and the mean age of the subjects was 54.2 years old. Thirty three percent of the subjects were diagnosed diabetes less than 2 years ago. The average nutrition knowledge score about diabetes was 10.2 point, and percentages of correct answers were very high in 'foods rich in fiber' (97.0%), 'relevance of exercise and insulin' (97.0%), 'quantity of insulin injection' (91.0%), and 'diabetes menu' (91.0%). The sources of nutrition information were hospitals/healthcare centers (56.1%), TV/radio (19.2%), and internet (13.1%). Sixty nine percent of the subjects have experienced nutrition education on subjects as 'menu planning skills'(22.4%), 'selecting foods' (22.4%), 'relevance of blood glucose and eating foods' (21.5%) by personal counseling (54.4%). The total score of eating behavior was higher after diagnosed diabetes (35.3) than before (30.0) (p < 0.001). The preferred topics in developing diabetes nutrition information websites were 'diabetes mellitus', 'relevance of blood glucose and foods', and 'selecting foods for diabetes'. The subjects wanted the websites developed by 'using mainly illustrations, pictures, tables' (22.8%) and 'using simple design' (19.6%). The preferred contents in developing diabetes self-management nutrition program were 'dietary life diagnosis', 'chronic disease risk diagnosis', 'calorie control by selecting foods and cooking skills', and 'dietary assessment'. In designing the program, the subjects' most wanted designs were 'be handy and simple in using' (29.3%), 'using simple design' (17.9%), and 'using mainly illustrations, pictures, tables' (15.7%).

임목재적(林木材積) 산정(算定)을 위(爲)한 Simple Random Sampling과 Two-stage P.P.S. Sampling 방법(方法)의 비교(比較) (Comparison of Simple Random Sampling and Two-stage P.P.S. Sampling Methods for Timber Volume Estimation)

  • 김재수
    • 한국산림과학회지
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    • 제65권1호
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    • pp.68-73
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    • 1984
  • Simple random sampling과 P.P.S. sampling의 효율을 비교하기 위하여 오스트리아 Salzburg 부근의 침엽수 장령림 임분에서 임목조사를 실시하였다. 축적 1:10,000의 흑백 적외선 사진을 판독하여, 조사 임지를 제지, 유령림, 너도 밤나무 장령림, 침엽수 장령림으로 구분하고, 침엽수 장령림 내에서 random sampling에 의한 99개의 표본점과 P.P.S. sampling에 의한 75개의 표본점을 흉고 단면적 제수 4인 Relascope에 의하여 야외 조사한 자료를 비교한 결과는 다음과 같다. 1) random sampling에 의한 임분 재적의 추정치는 $422.0m^3/ha$이었고, P.P.S. sampling에 의해서는 $433.5m^3/ha$이었으나 이들간의 통계적 유의성은 없었다. 2) 5 %의 허용 오차내에서는 P.P.S. sampling에 의하여는 170점, random sampling에 의하여는 237점이 필요하였다. 3) P.P.S. sampling은 random sampling에 비하여 야외 조사 시간을 17% 감소시킬 수 있었다.

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SIMPLE VALUATION IDEALS OF ORDER TWO IN 2-DIMENSIONAL REGULAR LOCAL RINGS

  • Hong, Joo-Youn;Lee, Hei-Sook;Noh, Sun-Sook
    • 대한수학회논문집
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    • 제20권3호
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    • pp.427-436
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    • 2005
  • Let (R, m) be a 2-dimensional regular local ring with algebraically closed residue field R/m. Let K be the quotient field of R and v be a prime divisor of R, i.e., a valuation of K which is birationally dominating R and residually transcendental over R. Zariski showed that there are finitely many simple v-ideals $m=P_0\;{\supset}\;P_1\;{\supset}\;{\cdotS}\;{\supset}\;P_t=P$ and all the other v-ideals are uniquely factored into a product of those simple ones. It then was also shown by Lipman that the predecessor of the smallest simple v-ideal P is either simple (P is free) or the product of two simple v-ideals (P is satellite), that the sequence of v-ideals between the maximal ideal and the smallest simple v-ideal P is saturated, and that the v-value of the maximal ideal is the m-adic order of P. Let m = (x, y) and denote the v-value difference |v(x) - v(y)| by $n_v$. In this paper, if the m-adic order of P is 2, we show that $O(P_i)\;=\;1\;for\;1\;{\leq}\;i\; {\leq}\;{\lceil}\;{\frac{b+1}{2}}{\rceil}\;and\;O(P_i)\;=2\;for\;{\lceil}\;\frac{b+3}{2}\rceil\;{\leq}\;i\;\leq\;t,\;where\;b=n_v$. We also show that $n_w\;=\;n_v$ when w is the prime divisor associated to a simple v-ideal $Q\;{\supset}\;P$ of order 2 and that w(R) = v(R) as well.

Effects of surrounding and underlying shades on the color adjustment potential of a single-shade composite used in a thin layer

  • Mariana Silva Barros;Paula Fernanda Damasceno Silva;Marcia Luciana Carregosa Santana;Rafaella Mariana Fontes Braganca;Andre Luis Faria-e-Silva
    • Restorative Dentistry and Endodontics
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    • 제48권1호
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    • pp.7.1-7.10
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    • 2023
  • Objectives: This study aimed to evaluate the surrounding and underlying shades' effect on the color adjustment potential (CAP) of a single-shade composite used in a thin layer. Materials and Methods: Cylinder specimens (1.0 mm thick) were built with the Vittra APS Unique composite, surrounded (dual specimens) or not (simple specimens) by a control composite (shade A1, A2, or A3). Simple specimens were also built only with the control composites. Each specimen's color was measured against white and black backgrounds or the simple control specimens with a spectrophotometer (CIELAB system). The whiteness index for dentistry (WID) and translucency parameters (TP00) were calculated for simple specimens. Differences (ΔE00) in color between the simple/dual specimens and the controls were calculated. The CAP was calculated based on the ratios between data from simple and dual specimens. Results: The Vittra APS Unique composite showed higher WID and TP00 values than the controls. The highest values of ΔE00 were observed among simple specimens. The color measurements of Vittra APS Unique (simple or dual) against the control specimens presented the lowest color differences. Only surrounding the single-shade composite with a shaded composite barely impacted the ΔE00. The highest CAP values were obtained using a shaded composite under simple or dual specimens. Conclusions: The CAP of Vittra APS Unique was strongly affected by the underlying shade, while surrounding this composite with a shaded one barely affected its color adjustment.