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Immunoelectrophoretic analysis of major component proteins In cystic fluid of Taenia solium metacestodes (면역전기영동법에 의한 유구낭미충 낭액의 구성 단백질 분석)

  • Yoon Kong;Seung-Yull Cho;Suk-Il Kim;Shin-Yong Kang
    • Parasites, Hosts and Diseases
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    • v.30 no.3
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    • pp.209-218
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    • 1992
  • When cystic fluid of Taenia solium metacestodes (CF) was filtrated through Sephacryl S-300 Superfine, major proteins were in fractions III add IV Major protein in fraction III was Band C protein of 150 kDa and that in fraction IV was Band N protein (Choi et of., 1990). When CF was electrophoresed in 0.9% agarose gel and reacted with anti-CF rabbit serum (RACF), two main bands, a long outer and a short inner band, were precipitated, together with 8 minor bands. RACF reacted with fraction III forming the long outer band whereas RACF formed the short infer band with fraction IV in immunoelectrophoresis (IEP) The long outer precipitin band of CF fraction III was similar to antigen B in hydatid fluid (HF) of Oriol et at. (1971), while the short inner band of CF fraction IV was similar to HF antigen 5 of Caption et at. (1967) . When HF was reacted with RACF, the short inner band was immunoprecipitated without forming the long outer band. Common antigenicity between CF and HF seemed to exist in fraction IV rather than in fraction III of CF. Patient sera of neurocysticercosis reacted more frequently with fraction III than with fraction IV.

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Property of Uljin-Geumgangsong Wood (Pinus densiflora forma erecta Uyeki): Appearance Pattern of Resin Canal and Ray (울진 금강송재의 재질: 수지구 및 방사조직의 출현형태)

  • Kim, Dong-Woo;Hwang, Sung-Wook;Lee, Won-Hee
    • Journal of the Korean Wood Science and Technology
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    • v.42 no.1
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    • pp.78-87
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    • 2014
  • It was examined the key criterion of the time of property revelation as Uljin-Geumgangsong wood. For this purpose, we investigated in physical properties in pine wood (Pinus densiflora Sieb. et Zucc.) and representative Uljin-Geumgangsong wood (Pinus densiflora forma erecta Uyeki). The results are as follows; Air-dry density of Uljin-Geumgangsong wood have higher values over about 20 percent than that of pine wood. And also heartwood rate of Uljin-Geumgansong wood have higher values above 70 percent. The number of vertical resin canals per $1mm^2$ in Uljin-Geumgangsong wood was 40 percent greater than in pine wood. As the average diameters of axial resin canals were 67.8 mm and 67.3~69.9 mm in pine wood and in Uljin-Geumgangsong wood, respectively, there was no significant difference among wood species. There tends to be an increase in diameters as annual rings increase. The number of horizontal resin canals in Uljin-Geumgangsong-1 was approximately 55 percent greater than in pine wood and Uljin-Geumgangsong-2. The number of resin canals in pine wood and Uljin-Geumgangson-2 decreased while it increased in Uljin-Geumgangsong-1 as annual rings increased. No significant difference was found in the number of rays among wood species and between earlywood and latewood of same wood species. As annual rings increased the number of rays decreased in both pine wood and Uljin-Geumgangsong. The heights of rays were greater in pine wood than Uljin-Geumgangsong and no difference was found between earlywood and latewood. In conclusion, rings of tree for the time of property revelation as Uljin-Geumgangsong wood is assumed about more than 150 years.

FeO, $TiH_2$, Carbon 원료분말을 이용한 Fe-TiC 나노 복합분말 제조 및 소결

  • An, Gi-Bong;Kim, Ji-Sun
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2011.10a
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    • pp.26-26
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    • 2011
  • Fe계 TiC 합금은 미량의 합금원소를 첨가시켜 경화능, 내식성, 내마모성 성질을 개선한 특수 공구용 재료로서 현재 절삭, 내마모성, 광산, 금형재료 등의 분야에 널리 사용되고 있다. 금속과 세라믹의 복합재료인 초경합금은 비열처리용 공구강으로 WC, TiC 등의 4, 5, 6족 금속탄화물에 Co, Ni, Fe등의 철족이 결합금속으로 소결한 복합재료로 WC-Co계 초경합금이 주종을 이루고 있으나, 전략 소재로서 고가인 Co 원료를 대체하기 위한 재료로서 초경재료의 고경도와 공구강의 경제성 및 가공성의 장점을 이용한 Fe-TiC계 초경합금의 연구가 다양하게 진행되고 있다. 본 연구에서는 Fe기지에 서브마이크론 크기의 미세한 TiC 입자가 균일하게 분산된 Fe-TiC 복합분말을 경제적으로 제조하기 위해 순수한 Fe, Ti 원료분말에 비해 단가가 낮고 미세 분쇄가 용이한 FeO, $TiH_2$ 분말을 고에너지 밀링 후 반응 열처리 시키는 유사 기계화학적 공정을 시도하였다. 조성비 Fe-30wt%TiC 복합분말을 제조하기위해 마이크론(micron) 크기의 FeO, $TiH_2$, C 분말을 사용하였고, 1단계로 FeO와 C을 고에너지 밀링으로 혼합 후 반응시켜 환원시키는 공정과 2단계로 이렇게 환원된 분말과 TiH2를 고에너지 밀링으로 다시 혼합, 분쇄한 후 반응열처리 하는 두 단계 공정을 사용하였다. FeO의 환원 단계에서는 $700{\sim}1,000^{\circ}C$ 온도 범위에서 1시간 유지하였고, 고에너지 밀링 시 밀링시간, 회전속도를 변수로 두고 실험하였다. 환원된 분말은 수평관상로를 이용해 아르곤분위기에서 $1,000{\sim}1300^{\circ}C$까지 1시간 유지하여 반응열처리시켜 Fe-TiC 복합분말을 제조하였다. 준비된 복합분말을 XRD와 FE-SEM, EDS, 입도분석기 (LPSA) 등을 이용해 분말의 형태와 특성, 상, 조성, 입도, 분산도 등을 조사하였다. 제조된 Fe-TiC 나노복합분말을 방전플라즈마소결(SPS) 과 상압소결 실험을 진행하였다. Fe-TiC 복합분말 제조공정의 첫 번째 단계인 FeO의 환원반응은 $800^{\circ}C$이상의 온도에서 Fe로 환원이 진행됨을 확인하였다. 두 번째 단계인 반응열처리공정에서는 $1,000^{\circ}C$ 이상에서 TiC가 형성됨을 XRD 상분석을 통해 확인할 수 있었고, $1,100^{\circ}C$ 이상의 온도에서 반응열처리를 했을 때 XRD 분석결과와 산소 조성 분석 결과로부터 반응의 완결성과 순도에서 최적 온도 조건임을 확인하였다. 온도를 $1,300^{\circ}C$로 증가시킬 경우 반응의 완결성에 큰 변화가 없는 반면 분말입자간의 목형성이 일어나 가소결 되는 것을 FE-SEM을 통해 관찰하였다. 또한 최적조건으로 제조된 Fe-TiC 복합분말의 입도분석과 FE-SEM/EDS 관찰/분석을 시행한 결과 평균 입도 0.6 ${\mu}m$의 미세한 Fe-TiC 복합분말 내에 Fe분말 주변과 내부에 나노크기의 TiC입자가 균일하게 분산되어 존재하는 것을 확인하였다.

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The Roles of Gold Plate (140${\mu}{\textrm}{m}$) Loaded on TLD-100 Chips in the High Energy Radiation Beams (고에너지 광자선속에서 TLD-100 chip 위에 있는 금박막(140 ${\mu}{\textrm}{m}$) 역할)

  • Vahc, Young-Woo;Park, Kyung Ran.
    • Progress in Medical Physics
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    • v.6 no.2
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    • pp.51-60
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    • 1995
  • Lithium Fluoride (LiF; TLD-100) crystal chips are normally used as thermolu minescence dosimeters (abbreviated as NC-100) for estimating the absorbed dose to the skin of a patient or in a solid water phantom undergoing radiotherapy with megavoltage photon (6 and 15MV) beams. In general, investigation has revealed a reduction in the sensitivity of NC-100 chips after many runs through heating cycles. A TLD-100 chip laminated with gold plate (140${\mu}{\textrm}{m}$) on the upper surface layer of its face toward the photon beam (abbreviated as GC-100) has properties different from that of a NC-100 chip activated by incident photons and contaminant electrons with various lower energies coming from the gantry head and air. Activation of the valence band electrons of GC-100 chips by incident photons, positrons and electrons-which come from the gold plate by mainly pair production process and partly from Compton scattering-results in more enhanced signal intensity, higher response per monitor unit, as well as a good linearity with monitor units and independence of dose rate. Since the electron beams (6 and 15 MeV) do not have the probability of pair production process with gold plate, there is only a small difference (about a 3.3% increase for 15 MeV) in the signal gaps in the TL readout for electron beams between GC- and NC-100 chips. The 3.3% increase is entirely due to the buildup caused by the 140 m gold plate. The sensitivity of GC-100 chips is much more susceptible to high energy photon beams than electron one because of pair production. The interaction of high energy photon with a material of high atomic number, such as the good plate in this case, results in a considerably significant probability of pair production. The gold plate on the NC-100 chips acts as not only an intensifier of their signals but also acts as a filter of contaminant electrons in therapeutic high energy X-ray beams.

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A Grain Size Analysis of Bottom Sediments of Yeongil Bay, Korea (한국 영일만 해저퇴적물의 입도분포)

  • Park, Byong-Kwon;Song Moo-Young
    • 한국해양학회지
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    • v.7 no.2
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    • pp.74-85
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    • 1972
  • This paper studied the grain size distribution of bottom sediments of Yeongil Bay which is located at the southeastern part of the Korean Peninsula. Sixty four samples collected with snapper and dredger are analyzed by roe Tap Sieve Shaker and Pipette Method. The moment parameters are calculated with the method of Friedman(1961). Most samples are composed of sand size sediments and a few samples are composed of silt and clay. The Yeongil Bay can be divided into gravel-granule zone, sand zone, and silt-clay zone. The sediments near Yeonam- Dong and Hyongsan river are moderately sorted and others are very poorly sorted according to scheme of Friedman91962). In general, sorting values are ranged from 1.0 to 3.5. The samples near Janggigap and Masin-Dong show negative and others show positive skewness values. Skewness values are ranged from -1 to 2. All samples show the leptokurtic distribution except for the samples near Masin- dong and at the deepest place near Janggigap. Kurtosis values are ranged from -1.5 to 21.9. The samples of gravel-granule zone contain more than 50% and those of silt-clay zone contain less than 50% of CaCO$\_$3/. Four different colors, black, yellow, brown and gray, are shown in the sediments of Yeongil Bay.

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Preparation and Properties of Coimmobilized Glucose Oxidase-Catalase (Glucose Oxidase와 Catalase의 동시 고정화 제품과 성질)

  • Lee, Suk-Hee;Uhm, Tai-Boong;Cho, Sook-Ja;Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.27 no.3
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    • pp.180-186
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    • 1984
  • For the study of glucose oxidase(GOD) and catalase(CAT) coimmobilization system, the enzymes were obtained from Penicillium spp., PS-8, and the strain itself was used as an immobilizing matrix. To separate glucose oxidase and catalase after the ammonium sulfate fractionation of the culture broth, DEAF-cellulose column was used and its activity yield was 54 and 34%, respectively. Both enzymes were immobilized on the cell matrix, followed crosslinking with 2.5% glutaraldehyde for 12hr. In the determination of efficiencies of GOD and CAT of dual, mixed and soluble enzyme systems, the dual immobilized one w-as superior to those of the soluble or mixed ones. In the comparison of pH profiles, the dual and mixed types showed broader maximum pH ranges than the soluble type. Varying CAT/GOD ratio of the dual system, the higher the ratio showed the broader activity profile. In the comparison of apparent $K_m$ of GOD only and CAT/GOD=10, they were $7.1{\times}10^{-2}$ and $5.1{\times}10^{-2}M$. Their activation energies showed 3.98kcal/mole/deg for GOD only and 2.98kcal/mole/deg for CAT/GOD=10.

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The Effect of Ionic Group and MMA Contents on the Physical Properties of PU/PMMA Hybrids (PU/PMMA Hybrids의 물성에 대한 이온성기와 MMA함량의 영향)

  • Jeong, Chang Nam;Cho, Hang Kyu;Noh, Si Tae
    • Applied Chemistry for Engineering
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    • v.8 no.4
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    • pp.575-581
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    • 1997
  • UDs were synthesized from two different polyols(PTMG, PBEAG), ionic chain extender(DMPA), EDA with $H_{12}-MDI$. PU/PMMA hybrids were prepared with free radical polymerization of MMA monomer in MMA-swelled PUD. PUD particle size and film properties were investigated ionic content and polyol type. Mechanical and thermal properties of PU/PMMA hybrid film were studied in terms of PU's ionic content and the venation of PU/PMMA compositions. As DMPA content increased from 2wt% to 10wt% in PUD, particle size of PUD decreased. PUD's particle size with ester type polyol was found to be smaller then ether type polyol used. Phase separation between hard segment(HS) and soft segment(SS) with ionic contents in PU was shown by the thermal, mechanical property measurement. Although the composition of MMA was changed from 0 to 40 wt% in PU/PMMA hybrid, the particle size of the hybrid did not increase. Using the ester type polyol, tensile strength of hybrid was found to increase by 2wt% - 6wt% DPMA content, but as higher content the strength of hybrid decreased. Moreover with the ether type polyol, tensile strength of hybrid was observed to increase by 2wt% - 4wt% DMPA content, while decreasing at higher content. PU and PMMA polymer molecule being mixed in molecular level was confirmed from the pattern of $T_g$ in DSC thermogram.

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A Study on the Effects of Biodegradation for Organic Soils (유기질토에 대한 생분해처리 효과에 관한 연구)

  • Song, Yeong-U;Park, Jun-Beom;Kim, Hyeong-Seok
    • Geotechnical Engineering
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    • v.14 no.4
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    • pp.151-162
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    • 1998
  • The compressibility and the permeability of organic soil are so high that they cause many engineering problems when constructing a structure on the soil. If the organic content of the soil could be reduced by any applicable engineering technique, the engineering properties of the soils can be improved to some extent. The purpose of this study would thus be focused on how to decrease the amount of organic matters by applying aerobic biodegradation for eliminating post-construction settlement problems. To enhance the aerobic decomposition, oxygen was supplied to the soil samples prepared by the mixture of kaolinite and sawdust as organic matter. The dissolved oxygen and the organic content of the soil samples were measured, in accordance with the passage of time through the bests. As oxygen suppliers, HaOa liquid and pure oxygen gas were compared to meet the requirement of the test purposes. Newly manufactured oedometer with the diameter of 130 mm and the height of 300 mm was used for 100 days to perform the compressibility tests for the soils. Based on the results of this experiment, the oxygen gas-treated samples with nutrient settled 30% more than the samples untreated. This confirmed the efficiency of the aerobic biodegradation. $NaNO_3$ added into the soils as nutrients was proved more effective than $K_2HP0_4$. To confirm the activity of micro-organisms, sodium azide was also added to the soils.

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Effects of Aggregate Size and Steel Fiber Volume Fraction on Compressive Behaviors of High-Strength Concrete (골재크기 및 섬유혼입률에 따른 강섬유 보강 고강도 콘크리트의 압축거동)

  • Ahn, Kyung-Lim;Jang, Seok-Joon;Jang, Sang-Hyeok;Yun, Hyun-Do
    • Journal of the Korea Concrete Institute
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    • v.27 no.3
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    • pp.229-236
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    • 2015
  • This paper describes the effect of aggregate size on compressive behavior of high-strength steel fiber reinforced concrete. The Specified compression strength is 60 MPa and the range of fiber volume fraction is 0~2%. The main variable is the aggregate size, which was used for the aggregate size of 8 and 20 mm. So, ten concrete mixtures were prepared and tested to evaluate the fresh and hardened properties of SFRC at curing ages (7, 14, 28, 56 and 91 days), respectively. Items estimated in this study are the fresh properties (air contents, slump), hardened properties (compressive strength, modulus of elasticity, post-peak response and compressive toughness). As a result, the aggregate size has little effect on the compressive strength and modulus of elasticity. On the other hand, the ductile behavior was shown after post peak and the compressive toughness was increasing as decreasing the aggregate size. These effects are clearly represented in the fiber volume fraction 2%, which are the point appeared fiber ball. It is considered that the decreasing the aggregate size has effect on the fiber dispersibility.

Synthesis and Magnetic Properties of $Fe-TiO_2$ Nanocomposite Powders by Mechanical Alloying (기계적 합금화법에 의한 $Fe-TiO_2$ Nanocomposite의 합성 및 자기적 성질)

  • 홍대석;이성희;이충효;김지순;권영순
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2002.11a
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    • pp.63-63
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    • 2002
  • 현재 기계적 합금화법에서는 주로 합금을 구성하는 성분원소 분말을 불활성분위기에서 볼밀처리 함으로써 함금화를 시키거나 모합금에 산화물을 분산시켜 복합화시키는 공정을 통하여 각종 화합물, 비정질상 및 과포화고용체등의 준안정상의 합성 뿐만이 아니라 초미세조직의 생성에 관한 폭 넓은 분야의 연구가 행하여지고 있다. 한편 MA에서는 볼멀처리중 기계적 에너지의 투여에 의하여 실제 반응온도보다 낮은 온도에서 발생하는 특이한 화학반응 즉 Mechanochemical 반응을 일으키 기도 한다. 본 연구에서는 헤마타이트($Fe_20_3$)와 금속윈소인 Ti의 MA처리에 의하여 고상환원반응 을 유기시켜 $Fe-TiO_2$계 nanocomposite 분말재료를 제조하고자 한다. 특히 MA 공정에 있어서 자기 물성의 변화와 X선 회절을 통하여 고상환원반응에 의한 복합분말의 생성과정을 조사하였다. 출발원료는 $Fe_20_3$(고순도화학제,99.9%, 평균입경 0.1$\mu\textrm{m}$)와 금속원소인 Ti(99.9%, 명균업경 150$\mu\textrm{m}$)을 몰비 2:3의 조성이 되도록 하여 MA를 실시하였다. 볼멀은 고에너지 유성형 볼밀장치(독일 제, Fritsch P-5)를 이용하였으며 진공치환형 용기에 원료분말을 장입하여 2회정도 진공배기한 후 아르곤 가스를 충전하여 볼밀을 행하였다. 얻어진 분말시료에 대하여 x-선 회절장치, 전자현미경 (SEM) 및 진동시료형자력계(VSM)를 통하여 결정구조, 미셰조직 빛 자기특성을 조사하였다. $Fe_2O_3-Ti$ 혼합분말의 MA처리 에 의하여 초기단계부터 환원반응과 함께 $Fe_3TIO_{lO}$ 중간상이 관찰 되었으나 30hrs의 MA처리 후 Fe와 산화물 $TiO_2$로 모두 환원되어 $Fe-TiO_2$계 나노복합분말이 얻어짐을 알 수 있었다. 이 때 X션 회절피크의 line broadening으로부터 복합분말의 Fe 명균 결정립 크기는 24nm로 초미세 결정럽의 분말합금이었다. 포화자화값은 볼밀처리에 따라 점점 증가하여 MA 30시간에는 20.3emu/g로 포화됨을 알 수 있었다. 또한 보자력 Hc는 MA초기단계에 350e로 매우 낮으나 30시간 후에는 Hc값이 2600e로 매우 큰 값을 나타내었다. 이것은 환원반응결과 초기에 생성된 Fe의 결정립이 비교적 크고 결정결함이 적으나 볼밀처리를 30시간까지 행하면 Fe 결정렵의 미세화 빛 strain 증가로 magnetic hardening이 일어나기 때문인 것으로 사료된다.

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