• Food Science of Animal Resources
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• v.20 no.4
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• pp.257-263
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• 2000

본 연구는 체중 18~19kg(16~17개월령)의 측염소 3 두(♀)를 반도체로 한 후 흑염소육에 한약재를 첨가한 것을 가약 흑염소 소주로 하고, 흑염소육만 증탕한 것을 순 흑염소 소주로 하였고, 한약재만 증탕한 것을 한약재 증탕액으로 하여 4$^{\circ}C$에 30일간 저장하면서 VBN, 아미노산 무기질 조성 변화를 분석하여 다음과 같은 결과를 얻었다. VBN함량은 모든 처리구에서 저장기간이 경과하면서 증가하는 경향이었고, 가약 흑염소 소주보다 순 흑염소 소주의 VBN이높게 나타났으며(P<0.05) 처리구 모두 실험기간 동안 가식권 범위내에 있었다. 무기물 함량은 가약 흑염소소주가 순 흑염소 소주보다 높은 함량을 나타내었고, 한약재 증탕액에서 높은 함량을 나타내었던 무기물이 가약 흑염소 소주에서 높은 함량을 나타내었다. 특히 가약 흑염소 소주는 순 흑염소 소주도다 Ca, K, Mg가 2배이상 많은 함량을 나타내었다. 저장기간이 경과함에 따라 Ca, Fe는 감소하는 경향이었다. 순 흑염소 소주는 가약 흑염소소주보다 총 아미노산 함량이 높았으며, 가약 흑염소 소주는 glycine, glutamic acid, alanine, aspartic acid 순이었고, 순 흑염소 소주는 glycine, glutamic acid, alanine, aspartic acid, arginine 순이었으며, 한약재 증탕액은 gluta-mic acid와 aspartic acid 함량이 가장 높은 경향이었다.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.143-143
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• 2003

흑염소는 우리나라 고유의 재래가축으로 다른 축종에 비해 아직까지 재래의 특성이 남아있는 가축이다. 또한 흑염소는 다른 경제동물에 비해 체구가 작고 온순하여 다루기 쉽고, 번식력이 좋으며, 임신기간이 짧은 장점을 가지고 있어 생명공학이나 형질전환동물의 생산과 같은 학문연구를 위한 실험용 동물로 많이 이용되고도 있다. 가축개량의 한 방법으로 오랫동안 수정란이식 기술이 연구ㆍ이용되고 있으나 한우, 젖소, 돼지 등의 한정된 동물에게만 연구ㆍ개발 되어왔으며, 흑염소에서는 이러한 연구가 미진한 실정이다. 이에 본 실험에서는 흑염소의 체외수정란 생산기술을 개발하여 생명공학실험을 위한 기초 동물로서 유용하게 활용하며 고유의 유전자원인 흑염소의 보존을 위한 기반을 마련하고자 한다. 체외수정란생산을 위해 사용된 난소는 도축장에서 도축된 재래 흑염소의 난소를 도축 즉시 적출하여 penicillin G와 streptomycin이 함유된 3$0^{\circ}C$내외의 생리식염수에 담아 1~2 시간내에 실험실로 운반하였다. 운반된 난소는 항생제가 첨가된 생리식염수로 3~4회 세척하여 실험에 공시하였다. (중략)

• Food Science of Animal Resources
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• v.22 no.4
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• pp.301-309
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• 2002

This study was carried out to develop the breed-specific DNA markers for breed identification of Korean native goat meat using amplified fragment length polymorphism (AFLP)-PCR techniques. The genomic DNAs of Korean native goat, imported black goat and four dairy goat breeds(Saanen, Alpine, Nubian and Toggenburg) were extracted from muscle tissues or blood. Genomic DNA was digested with a particular combination of two restriction enzymes with 4 base(Mse I and Taq I) and 6 base(EcoR I and Hind III) recognition sites, ligated to restriction specific adapters and amplified using the selective primer combinations. In AFLP profiles of polyacrylamide gels, the number of scorable bands produced per primer combination varied from 36 to 74, with an average of 55.5. A total of 555 bands were produced, 149(26.8%) bands of which were polymorphic. Among the ten primer combinations, two bands with 2.01 and 1.26 kb in M13/H13 primer and one band with 1.65 kb in E35/H14 primer were found to be breed-specific AFLP markers in Korean native goat when DNA bands were compared among the goat breeds. In the E35/H14 primer combination, 2.19, 2.03, 0.96 and 0.87 kb bands detected in imported black goat, 2.13 kb band in Saanen breed and 2.08 kb band in Nubian breed were observed as breed-specific bands showing differences between goat breeds, respectively. The E35/H14 primer combination produced four DNA bands distinguished between Korean native goat and Saanen breed. The is study suggested that the breed specific AFLP bands could be used as DNA markers for the identification of Korean native goat meat from imported black goat and dairy goat meats.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.10a
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• pp.103-106
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• 2005

흑염소 육의 조단백질 함량은 약 21,1%, 조지방 함량은 약 3.7%로 단백질 함량은 우육과 유사하였으나 지방함량이 다른 축종에 비해 낮게 나타났다. 지방산 중 포화지방산은 등심부위가 47.62% 볼기부위가 41.88%였으며 불포화지방산 중 oleic acid가 등심(46.59%) 및 볼기(50,29%)였다. 흑염소 육의 콜레스테롤 함량은 35.73${\sim}$37.48 mg/100g으로 우육 및 돈육과 비해 현저하게 낮게 나타났다. 흑염소 육의 아미노산은 glutamic acid가 가장 높게 나타났으며 필수아미노산 비율은 약 50.3%이었다. 미네랄 함량은 칼륨>인>나트륨>마그네슘>칼슘>아연 >철>구리>망간 순이었다. L-carnitine 함량은 20.8${\sim}$26.0mg/100g의 범위로 우육과 돈육보다 높게 나타났다. 결국, 흑염소육은 무기질 함량도 높으며 특이적인 생리활성 영양성분인 carnitine도 타육에 비해 높은 경향을 보여서 영양학적으로 저지방 고단백질의 육류로 판단되었다.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2008.09a
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• pp.77-89
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• 2008

본 연구는 유기흑염소 생산에 기초적인 자료를 얻고자 유형이 다른 산지 초지에 방목하여 흑염소의 발육과 육질에 미치는 영향을 조사하였다. 공시축은 흑염소 40두를 4처리구(개량목초구, 유기목초구, 산야초구, 수엽류구)로 나누어 처리구당 10두씩 공시하여 2007년 5월 15일부터 2007년 11월 5일까지 174일간 농촌진흥청 축산과학원 가축유전자원시험장 방목지에서 수행하였다. 그 결과는 다음과 같다. 일당증체량은 개량목초구가 다른 시험구 보다 유의하게 높았다(p<0.05). 도체율은 개량목초구와 유기목초구가 산야초구와 수엽류구 보다 유의하게 높게 나타났다(p<0.05). 정육율은 개량목초구가 유의하게 높게 나타났다(p<0.05). 흑염소 고기의 조지방은 산야초구가 높은 경향이었다. 고기의 리놀레산과 리놀렌산은 개량목초구 보다 유기목초구, 산야초 및 수엽류구가 높은 경향이었으며, 아라키돈산은 산야초와 수엽류구가 다른 시험구 보다 높은 경향으로 나타났다. 전단력은 산야초구가 낮은 경향이었으며, 관능검사 결과 중 다즙성과 연도는 유기목초구와 산야초구가, 풍미는 산야초구와 수엽류구가 우수한 경향으로 나타났다. 이상의 결과를 종합해 보면, 산지에 방목 사육된 유기흑염소는 다소 생산성과 도체특성은 낮으나 육질 및 물리적 특성이 우수한 경향으로 나타나 고품질 유기축산물 생산으로 농가소득 증대뿐만이 아니라 소비자 요구충족에도 가능성이 높은 것으로 기대된다.

• Journal of Embryo Transfer
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• v.22 no.1
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• pp.53-61
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• 2007

This study was investigated factors affecting the pregnancy rates after transfer of pronuclear microinjected embryos for the production of transgenic Korean black goats. Embryo transfer was carried out in 343 recipient Korean black goats from September 1999 to June 2000. Estrus was induced by the insertion of intravaginal progesterone devices $CIDR^(R)$ for 2 weeks. A single injection of 400 IU equine chorionic gonadotropin was administered at 48h before $CIDR^(R)$ removal to increase the proportion of does cycling and ovulation rate. Good quality embryos were prepared by microinjection of DNA into the pronuclei of fertilized goat oocyte and cultured in vitro. Pronuclear microinjected $1{\sim}8$ cell stage embryos were surgically transferred into the oviducts of the recipient at day 4 or 5 following $CIDR^(R)$ removal, and morula to blastocyst stage embryos were surgically transferred into uterus at day 9. Pregnancy was diagnosed by transrectal ultrasound scanning at $20{\sim}30d$ and 8 weeks following embryo transfer. The pregnancy rate was affected by several factors, such as estrus induction, the number of previous transfer, transfer site, stage of CL (corpus luteum), the number of recipient CL, stage of embryos and the number of transferred embryo. The pregnancy rate was significantly higher in recipients that came into estrus naturally than recipients that induced to come into estrus with $CIDR^(R)$(59.1% vs. 36.8%; P<0.05). The pregnancy rate was higher when the embryos were transferred into the left oviduct than transferred into the right oviduct (42.9% vs. 35.3%; P<0.05). The pregnancy rate of recipients with $CH_1$ (early) stage corpus hemorrhagicum in ovary was hi틴or than recipient with $CH_3$ (late) stage hemorrhagicum (47.5% vs. 17.9%; P<0.01). Higher pregnancy rates were obtained by transfer of 1-cell stage embryos into oviduct while late blastocysts (51.6% vs. 66.7%; P<0.01) into uterus. The pregnancy rates when 3 embryos were transferred to recipients were significantly higher than when 2 embryos we.e transferred (47.6% vs. 27.0%; P<0.05). Although there were no significant difference among the group, adhesion of reproductive organs, uterine size, ovulation rate of recipients, presence of large follicle and difficulty of transfer affected pregnancy rate of recipient. Higher pregnancy rates were obtained in the recipients with $8{\sim}15m$ diameter uterine horn as compared to the recipients with <5m diameter or >20mm diameter uterine hem (38.9%, 20% vs. 18.2%), in the recipients with large follicle in the ovulated ovary ipsilaterally (53.6% vs. 37.1%) and in the transfer which was carried out easily (39.2% vs. 27.8%, 0%). In conclusion, the high rate of pregnancy was achieved following transfer of pronuclear microinjected embryos when three or four 1-cell stage embryos were transferred into oviduct with $CH_1$ stage corpus hemorrhagicum in the ovary of recipient which came into estrus naturally.

• The Korean Journal of Food And Nutrition
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• v.18 no.2
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• pp.135-139
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• 2005

This study was designed to investigate the characterization of black goat meat and bone beverage containing honey of red ginseng. The crude proteins and moisture contents of black goat meat were higher than those of pork and beef, but the crude lipid content of black goat meat was lower than that of those. The potassium content of black goat meat was lower than that of chicken, but similar to the pork and beef. The sodium content of black goat meat was lower than that of chicken, pork and beef. The result of the sensory evaluation of black goat meat and bone beverage added honey with red ginseng by 25 panels, was that the beverages added $7.5\%\;and\;8.0\%$ honey with red ginseng were better than any other beverages. Thus, the results suggest that the black goat meat bone beverage added honey with red ginseng could be one of valuable drinks.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.2
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• pp.145-149
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• 2006

This study investigated the quality characteristics of meat from goat fed fermented grape feeds including chemical composition, physical and sensory properties. The hardness of the goat meat was decreased by feeding fermented grape diets, but no significant difference in the pH was observed between control group and fermented grape diet-fed group. Major minerals in goat meat were shown to be Ca, Na, K and Mg and total amino acid content was the highest in a group fed $20\%$ fermented grape-containing diet (T2) among experimental groups. Major fatty acids in goat meat were linoleic acid, oleic acid, palmitic acid, and stearic acid while the content of oleic acid in meat was significantly increased in the group fed fermented grape diet. By increasing the amount of fermented grape in goat diet, L (lightness), a (redness) and b (yellowness) color parameters of goat meat were increased. From the sensory test, the meat from goat fed $30\%$ fermented grape-containing diet (T3) was evaluated the best, with higher overall acceptability of meat at higher content of fermented grape in goat feeds.

• Journal of Life Science
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• v.30 no.11
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• pp.1007-1011
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• 2020

This study was conducted to analyze the genetic diversity and relationships that discriminate between Korean native black goat populations (Dangjin, Jangsu, Tongyoung, and Gyeongsang National University strains) and crossbred goats. Monomorphic single nucleotide polymorphisms (SNPs) in each strain were collected, and 133 common SNPs were selected for analysis. These 133 monomorphic SNPs showed differences in the genetic structure of the Korean native black goat and crossbred goats, and results from the principal component analysis (PCA) showed that the two can be clearly separated. Furthermore, analysis of the validation population comprising 70 individuals (Korean native black goats, n = 24; crossbred goats, n = 46) with the reference population showed that Korean native black goat strains and the reference population have the same genetic structure, and the crossbred goats shared only part of the genetic structure with the reference population. The result of the PCA analysis showed that the Korean native black goat strains form one population, whereas the foreign strains form another population which is more widely dispersed than the Korean native black goat strains. Thus, the results from this study can be used as baseline data for the conservation of genetic resources of Korean native black goat communities through utilization of monomorphic SNPs and for the introduction of exotic species for further improvement in genetic diversity. This study can also help reduce unnecessary inbreeding and gene flow between native strains.

• The Science & Technology
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• v.31 no.7 s.350
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• pp.94-95
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• 1998

백혈병.빈혈 등의 치료에 사용되는 물질을 생산하는 흑염소가 세계최초로 우리나라에서 탄생되었다.한국과학기술원 생물과학과 유욱준교수팀 등은 인체 백혈구 증식인자 G-CSF를 젖으로 분비하게 될 재래종 흑염소 메디(Meddy)를 유전자 형질전환 기법으로 만들어냈다.