• Korean Journal of Ichthyology
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• v.13 no.4
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• pp.274-278
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• 2001

The morphology is described of a post larva of Pomacanthus xanthometopon of the family Pomacanthidae collected in the West Pacific ($26^{\circ}9'N$, $125^{\circ}0'W$) on May 5, 2001. The specimen is moderate to deep bodied and laterally compressed. Body depth is 66.3% of body length at 8.0 mm BL. The body is covered with small spine groups, each composed of two to four spines. The anus located at a vertical through the 8th or 9th dorsal fin spine. There is no strong spine at the angle of the preopercle that appears in the adult stage. Small pigments are spread dorsally on the head, upper trunk and over the membrane of dorsal fin, from the 1st to 11th ray.

• Korean Journal of Ichthyology
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• v.23 no.3
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• pp.217-224
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• 2011

The seven leptocephali (14.6~68.0 mm in total length) of the family Ophichthidae, from the East Sea of Korea, were identified using 12S rRNA 861-bp sequences and their morphological development were described. Our seven leptocephali were identified to Echelus uropterus based on genetic distance (d=0.000) being well consistent with that of adult E. uropterus. Our seven leptocephali of E. uropterus are characterized by morphological combinations: caudal fin present; the melanophores present in series of nine gut swellings; total myomeres 154~158; body depth in total length less than 10%; eight black horizontal bands present from anus to caudal fin base. Our morphological and molecular results suggest that E. uropterus may be an intermediate group between subfamily Ophichthinae and Myrophinae.

• The Korean Journal of Ecology
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• v.22 no.3
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• pp.163-167
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• 1999

In order to investigate territorial behaviour, Pungitius sinensis kaibarae were collected from Sacheon-river, Kangwon-do, Korea. They are reared in aquarium with designed experimental region from March to May 1996. In this study, territorial behaviour was divided into 1) pre-territorial movements of a shoal under varying water-weeds position 2) individual ranking patterns in connection with total length of fish 3) the change in size of territorial maps during the parental stage. The movements of a shoal tend to prefer regions with water-weeds and the lower half of the aquarium. Competition for territory was fierce, and fish that are longer in total length dominate smaller one in occupying territory. Both males and females developed territorial behaviour as they grew. However, male's territory was enlarged according to the hatching and dispersion of fry. At this time, aggressive tendencies reached their peak.

• Korean Journal of Ichthyology
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• v.7 no.2
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• pp.101-108
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• 1995

Four specimens of Megalaspis cordyla(Linnaeus) belonging to the family Carangidae and three specimens of Champsodon snyderi Franz belonging to the family Champsodontidae were collected for the first time from Nam-hae and Pusan, Korea. Megalaspis cordyla is similar to other genera, but differs in some morphological charac-ters : the presence of finlets, the beginning portion and shape of scutes, and the length of pec-toral fin. A new Korean name "Ko-d$\breve{u}$ng-ka-ra-ji" is proposed for the M. cordyla. Champsodon snyderi is differs from C. capensis in the number of pectoral fins and the pres-ence of scale on cheek ; from C. longipinnis in having a pale spinous dorsal fin ; from C. guen-theri and C. machaeratus in having 11 lower gill rakers, 19 soft dorsal fin rays. A new Korean name "Ak-$\hat{o}$-chi" is proposed for the C. snyderi.

• Korean journal of applied entomology
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• v.53 no.4
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• pp.457-460
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• 2014

Toxicity of several insecticides was tested against the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), and adult Gymnosoma rotundatum (Diptera: Tachinidae) in a sweet persimmon orchard. H. halys was captured using black light traps and G. rotundatum adults were collected using sticky traps lured with an aggregation pheromone, methyl-(E,E,Z)-2,4,6-decatrienoate of Plautia stali. Five insecticides, namely, bifenthrin 2 WP, buprofenzine+dinotefuran (20+15) WP, clothianidin 8 SC, dinotefuran 10 WP, and thiamethoxam 10 WP, were moderately toxic to G. rotundatum, resulting in 55.3% to 74.3% mortality. Bifenthrin 2 WP caused 93.1% mortality at 72 h within cages as a residual toxicity, and others caused varied mortalities.

• Korean Journal of Ichthyology
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• v.10 no.2
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• pp.260-267
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• 1998

Three species of the order Perciformes collected from coastal waters of Cheju Island, Korea were documented with photographs, all of which are newly known to Korea. Nemipterus bathybius Snyder, 1911 of the family Nemipteridae is distinguished by seven anal soft rays and the most upper ray at the caudal fin elongated. Cirrhitichthys aureus (Temminck et Schlegel, 1842) of the family Cirrhitidae (New Korean name: Hwangbokdom-kwa), is characterized by the five to six light black spots on the dorso-Iateral sides, 12 dorsal soft rays and a dorsal fin without spots and bands. Parapercis aurantiaca D$\ddot{o}$erlein, 1884 of the family Pinguipedidae, has several taxonomic characters. The dorsal spines and the soft rays of the dorsal fins were connected without a notch. The five to six dark yellow bands with the same size of eye length were shown on the lateral region. A new Korean name Kinsilkkoridom is proposed for the Nemipterus bathybius, and Cirrhitichthys aureus, Hwangbukdom, and Parapercis aurantiaca, Hwangssangdonggari.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.16 no.3
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• pp.129-144
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• 2003

Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Rhizoma Bletillae on the basal melanogenic activities of B16／F10 mouse melanoma cells, and on the ${\alpha}$-MSH or forskolin-induced melanogenesis. Rhizoma Bletillae alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Rhizoma Bletillae also suppressed the increase of ${\alpha}$-MSH (100 nM) or forskolin (20 ${\mu}M$)-induced melanin content and tyrosinase activity. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. Pretreatment of the cells with Rhizoma Bletillae also inhibited the increase of forskolin(20${\mu}M$) induced the amount of tyrosinase protein and tyrosinase promoter activity. The results of DOPA staining revealed that pretreatment of the cells with Rhizoma Bletillae showed less intensity than B16 melanoma cells stimulated with ${\alpha}$-MSH or forskolin. These results suggest that Rhizoma Bletillae inhibits melanogenesis and abrogates ${\alpha}$-MSH and cAMP-induced melanogenesis in B16 melanoma cells.

• Korean Journal of Ichthyology
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• v.8 no.2
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• pp.1-9
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• 1996

Egg and larval stages of the rockfish, Sebastes thompsoni and S. pachycephalus pachycephalus, are described and illustrared. The size of these eggs when released were 1.27~1.33mm in S. thompsoni and 1.39~1.52mm in S. pachycephalus pachycephalus. The size of these hatching larvae when released were 3.02~3.12mm TL (total length) in S. thompsoni and 4.83~5.17mm in S. pachycephalus pachycephalus. Notochord flexion occured 7.56~8.00mm TL (17~20 days after hatching) in S. thompsorzi and 5.43~5.56mm in S. pachycephalus pachycephalus. Preflexion and flexion larvae (averaged 3.97~7.78mm TL) of S. thompsoni were distinguished from related species by pigmentation of the dorsal and ventral midlines of the tail. Larvae of S. pachycephalus pachycephalus were distinguished from related species by pigmentation of the dorsal and ventral midlines of the trunk. Transformation occured 14.22~15.86mm TL in S. pachycephalus pachycephalus.

• Korean Journal of Pharmacognosy
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• v.28 no.4
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• pp.264-270
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• 1997

This Study was carried out develop antitumor effect of the n-butanol soluble of fraction of Perilla frutescens on human skin melanoma cells. The antitumor activity of various fractions obtained form n-butanol soluble fraction of Perilla frutescens was evaluated in human skin melanoma cells. The antitumor activity of the n-butanol soluble fraction in human skin melanoma cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, neutral red (NR) assay and sulforhordamine B protein (SRB) assay of colorimetic assay methods. The light microscopic study was carried out to observe morphological changes of cultured human skin melanoma cells. These results were obtained follows; The fractions 5 and 6 of the n-butanol soluble fraction of P frutescens were shown significant antitumor activities. The number of human skin melanoma cells were decreased and tend to form cell cluster by treatment with actions 5 and 7 of the n-butanol soluble fraction of P. frutescens. The fraction 6 of the the n-butanol soluble fraction showed the highest antitumor activity on P. frutescens. These results suggest that the fraction 6 of the n-butanol soluble fraction of P. frutescens may be a valuable choice for the studies on the treatment of human skin tumors.

• YAKHAK HOEJI
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• v.57 no.1
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• pp.24-31
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• 2013

Although statins, inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, have been shown to increase melanin synthesis, the exact mechanism of this action is not fully understood. In this study we investigated the possible involvement of intracellular $Ca^{2+}$ signal in the mechanism of stimulation of melanin synthesis induced by lovastatin in B16 cells. Lovastatin stimulated the production of melanin in a dose-dependent manner in the cells. Treatment with mevalonate, FPP and GGPP, precursors of cholesterol, did not significantly suppress the lovastatin-induced melanin production, suggesting that inhibition of cholesterol synthesis may not be involved in the mechanism of the action of lovastatin. In addition, lovastatin did not significantly alter the cAMP concentration and the stimulated production of melanin by lovastatin was not significantly changed by treatment with H89, a potent inhibitor of protein kinase A, which demonstrates that cAMP pathway may not be involved. However, lovastatin increased intracellular $Ca^{2+}$ concentration in a dose-related fashion. Treatment with EGTA, an extracellular $Ca^{2+}$ chelator did not significantly alter the lovastatin-induced intracellular $Ca^{2+}$ increase and melanin synthesis, whereas intracellular $Ca^{2+}$ reduction with BAPTA/AM and intracellular $Ca^{2+}$ release blockers (dantrolene and TMB-8) completely blunted these actions of lovastatin. Taken together, these results suggest that the intracellular $Ca^{2+}$ release may play an important role in the lovastatin-induced stimulation of melanin synthesis in B16 cells. These results further suggest that lovastatin may be useful for the treatment of hypopigmentation disorders, such as vitiligo.